################################## # NO CUT ENZYMES ( 179 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AciI AclI AcsI AcyI AfaI AflII AflIII AgeI AhdI Alw44I AocI Aor51HI ApaI ApaLI ApoI AscI Asp718I AspEI AspHI AspI AviII AvrII BanI BbeI BbrPI BbsI BbvCI BcgI BclI BfaI BfrI BglI BglII BlnI BlpI Bpu1102I BpuAI BsaAI BsaHI BsaJI BsaWI BseAI BsgI BsiEI BsiHKAI BsiWI BsmI Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstF5I BstPI BstUI BstZ17I Bsu36I Cac8I CelII CfoI Cfr10I ClaI CpoI DdeI DraI DrdI DsaI EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I EcoRI EcoRV EcoT14I EcoT22I FauI FbaI FokI FseI FspI HaeII HhaI Hin1I HinP1I HincII HindII HindIII HpaI HphI KasI KpnI Ksp632I KspI MaeI MaeII MfeI MluI MroI MslI MspA1I MunI MvnI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI Psp1406I PstI PvuI PvuII RcaI RsaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfaNI SfcI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI StuI StyI SwaI TaqI Tth111I TthHB8I Van91I XbaI XcmI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 50 enzymes) # ################################### AluI 293 AGCT AG^CT AlwNI 158 CAGTCACTG CAG[ACGT][ACGT][ACGT]^CTG AseI 79 ATTAAT AT^TAAT AsnI 79 ATTAAT AT^TAAT Asp700I 342 GAAACCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AvaI 331 CCCGAG C^[CT]CG[GA]G BalI 324 TGGCCA TGG^CCA BamHI 216 GGATCC G^GATCC BanII 334 GAGCCC G[GA]GC[CT]^C BbvI 291 GCAGC GCAGC(8/12) BciVI 186 GTATCC GTATCC(6/5) BcnI 405 CCCGG CC^[GC]GG BmyI 334 GAGCCC G[AGT]GC[ACT]^C BpmI 11 CTCCAG CTGGAG(16/14) BsaBI 85 GATTTGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaI 434 GAGACC GGTCTC(1/5) BseRI 423 CTCCTC GAGGAG(10/8) BsiYI 64 CCTATTACTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BslI 64 CCTATTACTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BsmBI 17 GAGACG CGTCTC(1/5) BsmFI 73 GGGAC GGGAC(10/14) BsoBI 331 CCCGAG C^[CT]CG[GA]G Bsp1286I 334 GAGCCC G[AGT]GC[ACT]^C BstNI 13 CCAGG CC^[AT]GG BstXI 221 CCATTTATTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG DraII 376 AGGACCT [GA]G^G[ACGT]CC[CT] DraIII 137 CACGAAGTG CAC[ACGT][ACGT][ACGT]^GTG EaeI 324 TGGCCA [CT]^GGCC[GA] EcoO109I 376 AGGACCT [GA]G^G[ACGT]CC[CT] EcoRII 13 CCAGG ^CC[AT]GG Fnu4HI 291 GCAGC GC^[ACGT]GC HapII 406 CCGG C^CGG HgaI 19 GACGC GACGC(5/10) HpaII 406 CCGG C^CGG ItaI 291 GCAGC GC^[ACGT]GC MamI 85 GATTTGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MboII 306 GAAGA GAAGA(8/7) MluNI 324 TGGCCA TGG^CCA MscI 324 TGGCCA TGG^CCA MspI 406 CCGG C^CGG MvaI 13 CCAGG CC^[AT]GG NciI 405 CCCGG CC^[GC]GG NspI 178 ACATGC [GA]CATG^[CT] PleI 391 GAGTC GAGTC(4/5) PpuMI 376 AGGACCT [GA]G^G[AT]CC[CT] PshAI 153 GACCCCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PshBI 79 ATTAAT AT^TAAT TseI 291 GCAGC G^C[AT]GC Tsp509I 491 AATT ^AATT XmnI 342 GAAACCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 14 enzymes) # ################################### AvaII 104 GGACC G^G[AT]CC 377 GGACC BsmAI 17 GAGAC GTCTC(1/5) 434 GAGAC BsrDI 287 CATTGC GCAATG(2/0) 417 CATTGC BssKI 13 CCAGG ^CC[ACGT]GG 405 CCCGG BstYI 216 GGATCC [GA]^GATC[CT] 315 GGATCT MflI 216 GGATCC [GA]^GATC[CT] 315 GGATCT MseI 80 TTAA T^TAA 493 TTAA MwoI 282 GCCCACATTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC 294 GCTAAAAGAGC NlaIII 179 CATG CATG^ 312 CATG NlaIV 104 GGACCC GG[ACGT]^[ACGT]CC 216 GGATCC ScrFI 13 CCAGG CC^[ACGT]GG 405 CCCGG SmlI 199 CTTGAG C^T[CT][GA]AG 388 CTTGAG Tru9I 80 TTAA T^TAA 493 TTAA XhoII 216 GGATCC [GA]^GATC[CT] 315 GGATCT ######################################### # 3 OR MORE CUT ENZYMES ( 15 enzymes) # ######################################### AlwI BsrI Cfr13I DpnII HaeIII HinfI MaeIII MboI MnlI NdeII Sau3AI Sau96I TfiI Tsp45I TspRI