(Circular) (Six-base) MAPSORT of: pFN21AE1652.seq Check: 6741 from: 1 to: 5212 pFN21AE1652 5212 bp With 182 enzymes: SgfI * August 16, 2010 14:48 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3022 278 Size: 53 83 186 936 1486 2468 Fragments arranged by size: 2468 1486 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2555 1937 Size: 618 4594 Acc65I G'GTAC_C Cuts at: 2533 2533 Size: 5212 AclI AA'CG_TT Cuts at: 1563 3339 3712 1563 Size: 1776 373 3063 Fragments arranged by size: 3063 1776 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2099 2445 3275 4659 4927 1632 Size: 467 346 830 1384 268 1917 Fragments arranged by size: 1917 1384 830 467 346 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5015 AflIII A'CryG_T Cuts at: 1203 4111 5088 1203 Size: 2908 977 1327 Fragments arranged by size: 2908 1327 977 AgeI A'CCGG_T Cuts at: 4043 4043 Size: 5212 AhdI GACnn_n'nnGTC Cuts at: 3941 3941 Size: 5212 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5212 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5212 AlwNI CAG_nnn'CTG Cuts at: 2168 2955 4527 2168 Size: 787 1572 2853 Fragments arranged by size: 2853 1572 787 ApaLI G'TGCA_C Cuts at: 3269 4425 3269 Size: 1156 4056 ApoI r'AATT_y Cuts at: 1493 2521 2747 2899 5008 1493 Size: 1028 226 152 2109 1697 Fragments arranged by size: 2109 1697 1028 226 152 AseI AT'TA_AT Cuts at: 160 1032 2085 3766 160 Size: 872 1053 1681 1606 Fragments arranged by size: 1681 1606 1053 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5212 AvaI C'yCGr_G Cuts at: 1870 1943 2336 2537 1870 Size: 73 393 201 4545 Fragments arranged by size: 4545 393 201 73 BamHI G'GATC_C Cuts at: 2221 2542 2909 2221 Size: 321 367 4524 Fragments arranged by size: 4524 367 321 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2533 3988 618 Size: 325 217 627 746 1455 1842 Fragments arranged by size: 1842 1455 746 627 325 217 BanII G_rGCy'C Cuts at: 1391 2343 2531 1391 Size: 952 188 4072 Fragments arranged by size: 4072 952 188 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4241 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3403 3437 1903 Size: 34 1466 34 3678 Fragments arranged by size: 3678 1466 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2202 3104 4314 2202 Size: 902 1210 3100 Fragments arranged by size: 3100 1210 902 BclI T'GATC_A Cuts at: 1548 1548 Size: 5212 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3823 136 Size: 107 122 71 631 605 2151 1525 Fragments arranged by size: 2151 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5207 5207 Size: 5212 BlpI GC'TnA_GC Cuts at: 2629 2629 Size: 5212 Bme1580I G_kGCm'C Cuts at: 1790 3273 4429 1790 Size: 1483 1156 2573 Fragments arranged by size: 2573 1483 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5212 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2085 2156 2943 Size: 416 53 83 399 24 668 71 787 Cuts at: 2943 3901 442 Size: 958 1753 Fragments arranged by size: 1753 958 787 668 416 399 83 71 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5212 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2031 2031 Size: 5212 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2001 3872 1239 Size: 762 1871 2579 Fragments arranged by size: 2579 1871 762 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2225 2414 3337 4202 4500 2225 Size: 189 923 865 298 2937 Fragments arranged by size: 2937 923 865 298 189 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2433 3875 882 Size: 617 934 1442 2219 Fragments arranged by size: 2219 1442 934 617 BsaAI yAC'GTr Cuts at: 493 1204 3003 493 Size: 711 1799 2702 Fragments arranged by size: 2702 1799 711 BsaBI GATnn'nnATC Cuts at: 2094 5206 2094 Size: 3112 2100 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3019 3401 4036 275 Size: 53 83 186 936 1486 382 635 1451 Fragments arranged by size: 1486 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3645 4043 4317 4464 4875 3645 Size: 398 274 147 411 3982 Fragments arranged by size: 3982 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 4987 Fragments arranged by size: 4987 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2419 1186 Size: 1233 3979 BseYI C'CCAG_C Cuts at: 4415 4911 4415 Size: 496 4716 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 4639 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2662 3423 4451 1540 Size: 275 186 661 761 1028 2301 Fragments arranged by size: 2301 1028 761 661 275 186 BsiHKAI G_wGCw'C Cuts at: 3273 3358 4429 3273 Size: 85 1071 4056 Fragments arranged by size: 4056 1071 85 BsmI GAATG_Cn' Cuts at: 2723 2816 2723 Size: 93 5119 BsmBI CGTCTCn'nnnn_ Cuts at: 2016 2016 Size: 5212 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2343 2531 3273 3358 4429 1391 Size: 399 553 188 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 553 399 188 85 BspEI T'CCGG_A Cuts at: 4875 4875 Size: 5212 BspHI T'CATG_A Cuts at: 3101 4831 3101 Size: 1730 3482 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2567 844 Size: 1723 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3099 1394 Size: 429 1276 3507 Fragments arranged by size: 3507 1276 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2291 2421 3707 3881 65 Size: 1183 1043 130 1286 174 1396 Fragments arranged by size: 1396 1286 1183 1043 174 130 BsrFI r'CCGG_y Cuts at: 1700 1810 3856 4043 1700 Size: 110 2046 187 2869 Fragments arranged by size: 2869 2046 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5212 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5212 BssSI C'ACGA_G Cuts at: 3272 4284 3272 Size: 1012 4200 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5212 BstBI TT'CG_AA Cuts at: 4016 4016 Size: 5212 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5212 BstXI CCAn_nnnn'nTGG Cuts at: 2233 2233 Size: 5212 BstYI r'GATC_y Cuts at: 1972 2221 2320 2542 2909 3294 3311 4763 4849 Size: 249 99 222 367 385 17 1452 86 Cuts at: 4849 4861 5207 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 385 367 346 249 222 99 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 4660 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4473 Fragments arranged by size: 4473 683 56 BtsI GCAGTG_nn' Cuts at: 2484 2728 3522 3542 2484 Size: 244 794 20 4154 Fragments arranged by size: 4154 794 244 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5212 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5177 DraI TTT'AAA Cuts at: 2516 2516 Size: 5212 DraIII CAC_nnn'GTG Cuts at: 2147 2147 Size: 5212 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4219 809 Size: 507 2903 1802 Fragments arranged by size: 2903 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2329 2659 3548 Size: 54 1425 50 275 18 499 330 889 Cuts at: 3548 5177 8 Size: 1629 43 Fragments arranged by size: 1629 1425 889 499 330 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2659 1537 Size: 275 847 4090 Fragments arranged by size: 4090 847 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3142 1353 Size: 1789 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3799 4173 4319 387 Size: 1064 138 2210 374 146 1280 Fragments arranged by size: 2210 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2001 2099 2445 3275 3872 4659 4927 Size: 393 369 98 346 830 597 787 268 Cuts at: 4927 1239 Size: 1524 Fragments arranged by size: 1524 830 787 597 393 369 346 268 98 EcoO109I rG'GnC_Cy Cuts at: 2382 2382 Size: 5212 EcoRI G'AATT_C Cuts at: 2521 2899 2521 Size: 378 4834 FspI TGC'GCA Cuts at: 3718 3718 Size: 5212 HaeII r_GCGC'y Cuts at: 1678 4359 1678 Size: 2681 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2305 2337 3853 3885 3927 Size: 32 135 32 733 32 1516 32 42 Cuts at: 3927 3959 1373 Size: 32 2626 Fragments arranged by size: 2626 1516 733 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2556 2802 669 Size: 988 281 618 246 3079 Fragments arranged by size: 3079 988 618 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5212 HpaI GTT'AAC Cuts at: 2802 2802 Size: 5212 KpnI G_GTAC'C Cuts at: 2537 2537 Size: 5212 MfeI C'AATT_G Cuts at: 2811 2811 Size: 5212 MluI A'CGCG_T Cuts at: 5088 5088 Size: 5212 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2257 4326 4510 1305 Size: 952 2069 184 2007 Fragments arranged by size: 2069 2007 952 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2331 10 Size: 54 1425 343 499 2891 Fragments arranged by size: 2891 1425 499 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3170 3529 3688 518 Size: 648 2004 359 159 2042 Fragments arranged by size: 2042 2004 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2628 3305 4453 4698 1606 Size: 1022 677 1148 245 2120 Fragments arranged by size: 2120 1148 1022 677 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 4660 NdeI CA'TA_TG Cuts at: 387 387 Size: 5212 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5212 NotI GC'GGCC_GC Cuts at: 2659 2659 Size: 5212 NspI r_CATG'y Cuts at: 1132 1229 2187 2570 4115 1132 Size: 97 958 383 1545 2229 Fragments arranged by size: 2229 1545 958 383 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5212 PciI A'CATG_T Cuts at: 4111 4111 Size: 5212 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5212 PfoI T'CCnGG_A Cuts at: 2017 2409 2017 Size: 392 4820 PmeI GTTT'AAAC Cuts at: 2516 2516 Size: 5212 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3281 3313 4794 4826 3281 Size: 32 1481 32 3667 Fragments arranged by size: 3667 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2382 2382 Size: 5212 PshAI GACnn'nnGTC Cuts at: 1613 2162 1613 Size: 549 4663 PsiI TTA'TAA Cuts at: 2782 2782 Size: 5212 PstI C_TGCA'G Cuts at: 830 2564 830 Size: 1734 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5212 SalI G'TCGA_C Cuts at: 1936 2554 1936 Size: 618 4594 SbfI CC_TGCA'GG Cuts at: 2564 2564 Size: 5212 ScaI AGT'ACT Cuts at: 3460 5172 3460 Size: 1712 3500 SfcI C'TryA_G Cuts at: 826 1045 2560 3695 4376 4567 826 Size: 219 1515 1135 681 191 1471 Fragments arranged by size: 1515 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5212 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5212 SmaI CCC'GGG Cuts at: 1872 2539 1872 Size: 667 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2204 2393 3316 4217 4479 820 Size: 197 926 261 189 923 901 262 1553 Fragments arranged by size: 1553 926 923 901 262 261 197 189 SnaBI TAC'GTA Cuts at: 493 3003 493 Size: 2510 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5212 SphI G_CATG'C Cuts at: 1132 2570 1132 Size: 1438 3774 SspI AAT'ATT Cuts at: 5 52 3136 5 Size: 47 3084 2081 Fragments arranged by size: 3084 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5212 StyI C'CwwG_G Cuts at: 513 1065 2651 513 Size: 552 1586 3074 Fragments arranged by size: 3074 1586 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3239 3256 3409 3594 4039 325 Size: 1255 1659 17 153 185 445 1498 Fragments arranged by size: 1659 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3458 5170 96 Size: 274 80 33 51 1443 1481 1712 138 Fragments arranged by size: 1712 1481 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5172 5204 5172 Size: 32 5180 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5212 XbaI T'CTAG_A Cuts at: 2548 2548 Size: 5212 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5212 XmaI C'CCGG_G Cuts at: 1870 2537 1870 Size: 667 4545 XmnI GAAnn'nnTTC Cuts at: 2351 3341 2351 Size: 990 4222 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3020 276 Size: 53 83 186 936 1486 2468 Fragments arranged by size: 2468 1486 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI Bpu10I BsiWI BstZ17I Bsu36I EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI