################################## # NO CUT ENZYMES ( 164 enzymes) # ################################## AccI AccII AccIII AclI AflII AflIII AgeI AhdI Alw44I AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp700I AspEI AspHI AspI AvaI AviII AvrII BalI BamHI BbeI BbsI BbvCI BclI BcnI BfrI BglI BglII BlnI BlpI Bpu1102I BpuAI BsaBI BsaI BsaWI BseAI BsiEI BsiHKAI BsiWI BsmI BsoBI Bsp1407I BspDI BspEI BspLU11I BspMI BsrBI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstZ17I Bsu36I CelII CfoI Cfr10I ClaI CpoI DraI DraIII DrdI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoO65I EcoRI EcoRV EcoT14I EcoT22I FauI FbaI FseI FspI HaeII HapII HgaI HhaI HinP1I HincII HindII HindIII HpaI HpaII KasI KspI MaeIII MamI MfeI MluI MluNI MroI MscI MseI MslI MspI MunI MvnI NaeI NarI NciI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PflMI PinAI PmeI PshAI PshBI Psp1406I PvuI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI StyI SwaI Tru9I Tsp45I Tth111I Van91I XbaI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 48 enzymes) # ################################### AatII 681 GACGTC GACGT^C Acc65I 460 GGTACC G^GTACC AciI 234 CCGC CCGC(-3/-1) AcsI 611 AAATTC [GA]^AATT[CT] AcyI 681 GACGTC G[GA]^CG[CT]C AfaI 461 GTAC GT^AC AlwI 93 GATCC GGATC(4/5) AlwNI 337 CAGCTGCTG CAG[ACGT][ACGT][ACGT]^CTG ApoI 611 AAATTC [GA]^AATT[CT] Asp718I 460 GGTACC G^GTACC BanI 460 GGTACC G^G[CT][GA]CC BanII 254 GGGCTC G[GA]GC[CT]^C BbrPI 526 CACGTG CAC^GTG BcgI 606 GCATGAAATTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BciVI 311 GGATAC GTATCC(6/5) BfaI 661 CTAG C^TAG BmyI 254 GGGCTC G[AGT]GC[ACT]^C BpmI 84 CTGGAG CTGGAG(16/14) BsaAI 526 CACGTG [CT]AC^GT[GA] BsaHI 681 GACGTC G[GA]^CG[CT]C BseRI 124 CTCCTC GAGGAG(10/8) BsgI 132 CTGCAC GTGCAG(16/14) BsmAI 679 GAGAC GTCTC(1/5) BsmBI 679 GAGACG CGTCTC(1/5) Bsp1286I 254 GGGCTC G[AGT]GC[ACT]^C BspHI 767 TCATGA T^CATGA BsrDI 295 GCAATG GCAATG(2/0) BstXI 267 CCAACCTTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG BstYI 92 AGATCC [GA]^GATC[CT] DsaI 307 CCACGG C^C[GA][CT]GG EaeI 259 CGGCCA [CT]^GGCC[GA] EcoNI 142 CCTAAAGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG Hin1I 681 GACGTC G[GA]^CG[CT]C KpnI 460 GGTACC GGTAC^C MaeI 661 CTAG C^TAG MflI 92 AGATCC [GA]^GATC[CT] MspA1I 337 CAGCTG C[AC]G^C[GT]G MwoI 388 GCTCGAAATGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC NspI 443 ACATGC [GA]CATG^[CT] PleI 321 GACTC GAGTC(4/5) PmaCI 526 CACGTG CAC^GTG PmlI 526 CACGTG CAC^GTG PstI 61 CTGCAG CTGCA^G PvuII 337 CAGCTG CAG^CTG RcaI 767 TCATGA T^CATGA RsaI 461 GTAC GT^AC SfaNI 624 GATGC GCATC(5/9) XhoII 92 AGATCC [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 14 enzymes) # ################################### BsrI 83 ACTGG ACTGG(1/-1) 750 CCAGT DdeI 12 CTCAG C^T[ACGT]AG 335 CTCAG DpnII 93 GATC ^GATC 121 GATC DraII 52 GGGACCC [GA]G^G[ACGT]CC[CT] 596 GGGACCC EcoO109I 52 GGGACCC [GA]G^G[ACGT]CC[CT] 596 GGGACCC HphI 621 GGTGA GGTGA(8/7) 324 TCACC MboI 93 GATC ^GATC 121 GATC NdeII 93 GATC ^GATC 121 GATC PpuMI 52 GGGACCC [GA]G^G[AT]CC[CT] 596 GGGACCC Sau3AI 93 GATC ^GATC 121 GATC TaqI 104 TCGA T^CGA 390 TCGA TfiI 162 GATTC G^A[AT]TC 564 GATTC TspRI 656 CAGTG CA[GC]TG[ACGT][ACGT]^ 807 CACTG TthHB8I 104 TCGA T^CGA 390 TCGA ######################################### # 3 OR MORE CUT ENZYMES ( 32 enzymes) # ######################################### AluI AvaII BbvI BsaJI BsiYI BslI BsmFI BssKI BstF5I BstNI Cac8I Cfr13I EarI EcoRII Fnu4HI FokI HaeIII HinfI ItaI Ksp632I MaeII MboII MnlI MvaI NlaIII NlaIV Sau96I ScrFI SfcI TseI Tsp509I XcmI