(Circular) (Six-base) MAPSORT of: pFN21AE6668.seq Check: 1266 from: 1 to: 5593 pFN21AE6668 5593 bp With 182 enzymes: SgfI * March 18, 2015 14:53 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2690 3403 278 Size: 53 83 186 936 1154 713 2468 Fragments arranged by size: 2468 1154 936 713 186 83 53 AccI GT'mk_AC Cuts at: 1937 2936 1937 Size: 999 4594 Acc65I G'GTAC_C Cuts at: 2465 2914 2465 Size: 449 5144 AclI AA'CG_TT Cuts at: 1563 3720 4093 1563 Size: 2157 373 3063 Fragments arranged by size: 3063 2157 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3656 5040 5308 1632 Size: 2024 1384 268 1917 Fragments arranged by size: 2024 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5396 AflIII A'CryG_T Cuts at: 1203 4492 5469 1203 Size: 3289 977 1327 Fragments arranged by size: 3289 1327 977 AgeI A'CCGG_T Cuts at: 4424 4424 Size: 5593 AhdI GACnn_n'nnGTC Cuts at: 4322 4322 Size: 5593 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5593 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5593 AlwNI CAG_nnn'CTG Cuts at: 2347 3336 4908 2347 Size: 989 1572 3032 Fragments arranged by size: 3032 1572 989 ApaLI G'TGCA_C Cuts at: 3650 4806 3650 Size: 1156 4437 ApoI r'AATT_y Cuts at: 1493 2616 2902 3128 3280 5389 1493 Size: 1123 286 226 152 2109 1697 Fragments arranged by size: 2109 1697 1123 286 226 152 AseI AT'TA_AT Cuts at: 160 1032 4147 160 Size: 872 3115 1606 Fragments arranged by size: 3115 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5593 AvaI C'yCGr_G Cuts at: 1870 1943 2918 1870 Size: 73 975 4545 Fragments arranged by size: 4545 975 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2449 2482 2449 Size: 33 5560 BamHI G'GATC_C Cuts at: 2923 3290 2923 Size: 367 5226 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2465 2914 4369 618 Size: 325 217 627 678 449 1455 1842 Fragments arranged by size: 1842 1455 678 627 449 325 217 BanII G_rGCy'C Cuts at: 1391 2263 2912 1391 Size: 872 649 4072 Fragments arranged by size: 4072 872 649 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4622 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2600 2634 2872 2906 3784 3818 1903 Size: 34 663 34 238 34 878 34 3678 Fragments arranged by size: 3678 878 663 238 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2310 3485 4695 2310 Size: 1175 1210 3208 Fragments arranged by size: 3208 1210 1175 BclI T'GATC_A Cuts at: 1548 1548 Size: 5593 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4204 136 Size: 107 122 71 631 605 2532 1525 Fragments arranged by size: 2532 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5588 5588 Size: 5593 BlpI GC'TnA_GC Cuts at: 3010 3010 Size: 5593 Bme1580I G_kGCm'C Cuts at: 1790 3654 4810 1790 Size: 1864 1156 2573 Fragments arranged by size: 2573 1864 1156 BmgBI CAC'GTC Cuts at: 1318 2206 1318 Size: 888 4705 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3324 4282 442 Size: 416 53 83 399 24 1907 958 1753 Fragments arranged by size: 1907 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5593 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2110 4253 1239 Size: 871 2143 2579 Fragments arranged by size: 2579 2143 871 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3718 4583 4881 3718 Size: 865 298 4430 Fragments arranged by size: 4430 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4256 882 Size: 617 2757 2219 Fragments arranged by size: 2757 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2533 3384 493 Size: 711 1329 851 2702 Fragments arranged by size: 2702 1329 851 711 BsaBI GATnn'nnATC Cuts at: 5587 5587 Size: 5593 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2687 3400 3782 4417 Size: 53 83 186 936 1154 713 382 635 Cuts at: 4417 275 Size: 1451 Fragments arranged by size: 1451 1154 936 713 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4026 4424 4698 4845 5256 4026 Size: 398 274 147 411 4363 Fragments arranged by size: 4363 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5368 Fragments arranged by size: 5368 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2120 1186 Size: 934 4659 BseYI C'CCAG_C Cuts at: 4796 5292 4796 Size: 496 5097 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2122 1163 Size: 573 386 4634 Fragments arranged by size: 4634 573 386 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3043 3804 4832 1540 Size: 275 186 1042 761 1028 2301 Fragments arranged by size: 2301 1042 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3654 3739 4810 3654 Size: 85 1071 4437 Fragments arranged by size: 4437 1071 85 BsmI GAATG_Cn' Cuts at: 3104 3197 3104 Size: 93 5500 BsmBI CGTCTCn'nnnn_ Cuts at: 2678 2678 Size: 5593 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2263 2912 3654 3739 4810 1391 Size: 399 473 649 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 649 473 399 85 BspEI T'CCGG_A Cuts at: 5256 5256 Size: 5593 BspHI T'CATG_A Cuts at: 2772 3482 5212 2772 Size: 710 1730 3153 Fragments arranged by size: 3153 1730 710 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2948 844 Size: 2104 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3480 1394 Size: 429 1657 3507 Fragments arranged by size: 3507 1657 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2307 4088 4262 65 Size: 1183 1059 1781 174 1396 Fragments arranged by size: 1781 1396 1183 1059 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4237 4424 1700 Size: 110 2427 187 2869 Fragments arranged by size: 2869 2427 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5593 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5593 BssSI C'ACGA_G Cuts at: 3653 4665 3653 Size: 1012 4581 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5593 BstBI TT'CG_AA Cuts at: 4397 4397 Size: 5593 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5593 BstXI CCAn_nnnn'nTGG Cuts at: 2279 2279 Size: 5593 BstYI r'GATC_y Cuts at: 1972 2097 2923 3290 3675 3692 5144 5230 5242 Size: 125 826 367 385 17 1452 86 12 Cuts at: 5242 5588 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 826 385 367 346 125 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2312 513 Size: 552 939 308 3794 Fragments arranged by size: 3794 939 552 308 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4854 Fragments arranged by size: 4854 683 56 BtsI GCAGTG_nn' Cuts at: 3109 3903 3923 3109 Size: 794 20 4779 Fragments arranged by size: 4779 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5593 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5558 DraI TTT'AAA Cuts at: 2897 2897 Size: 5593 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4600 809 Size: 507 3284 1802 Fragments arranged by size: 3284 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2264 3040 3929 Size: 54 1425 50 275 18 434 776 889 Cuts at: 3929 5558 8 Size: 1629 43 Fragments arranged by size: 1629 1425 889 776 434 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3040 1537 Size: 275 1228 4090 Fragments arranged by size: 4090 1228 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2469 2579 2585 3523 1353 Size: 1116 110 6 938 3423 Fragments arranged by size: 3423 1116 938 110 6 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4180 4554 4700 387 Size: 1064 138 2591 374 146 1280 Fragments arranged by size: 2591 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2110 3656 4253 5040 5308 1239 Size: 393 478 1546 597 787 268 1524 Fragments arranged by size: 1546 1524 787 597 478 393 268 EcoNI CCTnn'n_nnAGG Cuts at: 2151 2151 Size: 5593 EcoO109I rG'GnC_Cy Cuts at: 2058 2602 2058 Size: 544 5049 EcoRI G'AATT_C Cuts at: 2902 3280 2902 Size: 378 5215 FspI TGC'GCA Cuts at: 4099 4099 Size: 5593 HaeII r_GCGC'y Cuts at: 1678 4740 1678 Size: 3062 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2214 2246 4234 4266 4308 Size: 32 135 32 642 32 1988 32 42 Cuts at: 4308 4340 1373 Size: 32 2626 Fragments arranged by size: 2626 1988 642 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2937 3183 669 Size: 988 281 999 246 3079 Fragments arranged by size: 3079 999 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5593 HpaI GTT'AAC Cuts at: 3183 3183 Size: 5593 KpnI G_GTAC'C Cuts at: 2469 2918 2469 Size: 449 5144 MfeI C'AATT_G Cuts at: 3192 3192 Size: 5593 MluI A'CGCG_T Cuts at: 5469 5469 Size: 5593 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2257 4707 4891 1305 Size: 952 2450 184 2007 Fragments arranged by size: 2450 2007 952 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3771 Fragments arranged by size: 3771 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3551 3910 4069 518 Size: 648 2385 359 159 2042 Fragments arranged by size: 2385 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2344 3009 3686 4834 5079 1606 Size: 738 665 677 1148 245 2120 Fragments arranged by size: 2120 1148 738 677 665 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4102 Fragments arranged by size: 4102 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5593 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5593 NotI GC'GGCC_GC Cuts at: 3040 3040 Size: 5593 NspI r_CATG'y Cuts at: 1132 1229 2452 2951 4496 1132 Size: 97 1223 499 1545 2229 Fragments arranged by size: 2229 1545 1223 499 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5593 PciI A'CATG_T Cuts at: 4492 4492 Size: 5593 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5593 PfoI T'CCnGG_A Cuts at: 2679 2679 Size: 5593 PmeI GTTT'AAAC Cuts at: 2897 2897 Size: 5593 PmlI CAC'GTG Cuts at: 2533 2533 Size: 5593 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3662 3694 5175 5207 3662 Size: 32 1481 32 4048 Fragments arranged by size: 4048 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2058 2602 2058 Size: 544 5049 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5593 PsiI TTA'TAA Cuts at: 3163 3163 Size: 5593 PstI C_TGCA'G Cuts at: 830 2070 2945 830 Size: 1240 875 3478 Fragments arranged by size: 3478 1240 875 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5593 PvuII CAG'CTG Cuts at: 2344 2344 Size: 5593 SalI G'TCGA_C Cuts at: 1936 2935 1936 Size: 999 4594 SanDI GG'GwC_CC Cuts at: 2058 2602 2058 Size: 544 5049 SbfI CC_TGCA'GG Cuts at: 2945 2945 Size: 5593 ScaI AGT'ACT Cuts at: 3841 5553 3841 Size: 1712 3881 SfcI C'TryA_G Cuts at: 826 1045 2066 2730 2845 2941 4076 4757 4948 Size: 219 1021 664 115 96 1135 681 191 Cuts at: 4948 826 Size: 1471 Fragments arranged by size: 1471 1135 1021 681 664 219 191 115 96 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5593 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5593 SmaI CCC'GGG Cuts at: 1872 2920 1872 Size: 1048 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3697 4598 4860 820 Size: 197 926 1754 901 262 1553 Fragments arranged by size: 1754 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3384 493 Size: 2891 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5593 SphI G_CATG'C Cuts at: 1132 2951 1132 Size: 1819 3774 SspI AAT'ATT Cuts at: 5 52 3517 5 Size: 47 3465 2081 Fragments arranged by size: 3465 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5593 StyI C'CwwG_G Cuts at: 513 1065 2004 3032 513 Size: 552 939 1028 3074 Fragments arranged by size: 3074 1028 939 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3620 3637 3790 3975 4420 325 Size: 1255 2040 17 153 185 445 1498 Fragments arranged by size: 2040 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3839 5551 96 Size: 274 80 33 51 1443 1862 1712 138 Fragments arranged by size: 1862 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2634 2666 5553 5585 2634 Size: 32 2887 32 2642 Fragments arranged by size: 2887 2642 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5593 XbaI T'CTAG_A Cuts at: 2929 2929 Size: 5593 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2358 2410 2746 2358 Size: 52 336 5205 Fragments arranged by size: 5205 336 52 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5593 XmaI C'CCGG_G Cuts at: 1870 2918 1870 Size: 1048 4545 XmnI GAAnn'nnTTC Cuts at: 3722 3722 Size: 5593 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2688 3401 276 Size: 53 83 186 936 1154 713 2468 Fragments arranged by size: 2468 1154 936 713 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI SanDI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BbeI BbvCI BfrBI BplI Bpu10I BsiWI BstZ17I Bsu36I DraIII EcoICRI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PspOMI PspXI PsrI RsrII SacI SacII SapI SexAI SfiI SfoI SrfI SwaI