################################## # NO CUT ENZYMES ( 122 enzymes) # ################################## AatII Acc65I AccIII AclI AflII AgeI AscI AseI AsnI Asp700I Asp718I AspI AviII AvrII BbrPI BbvCI BcgI BciVI BcnI BfaI BfrI BglII BlnI BlpI Bpu1102I BsaBI BsaWI BseAI BseRI BsgI BsiEI BsiWI BsmBI BsmI Bsp1407I BspDI BspEI BspHI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstXI BstZ17I CelII ClaI DraI DraIII DrdI EagI EclXI Eco52I EcoNI EcoRI EcoRV EcoT22I FseI FspI HincII HindII HindIII HpaI KpnI KspI MaeI MamI MfeI MluI MroI MseI MunI NciI NcoI NdeI NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI PvuII RcaI SacII SalI SapI SbfI ScaI SexAI SfuI SgrAI SmaI SnaBI SpeI Sse8387I SspBI SspI SwaI TaqI Tru9I Tth111I TthHB8I Van91I XbaI XcmI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 46 enzymes) # ################################### AcsI 988 AAATTC [GA]^AATT[CT] AfaI 177 GTAC GT^AC AhdI 67 GACTCGGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Alw44I 685 GTGCAC G^TGCAC AlwNI 915 CAGCACCTG CAG[ACGT][ACGT][ACGT]^CTG AocI 96 CCTCAGG CC^T[ACGT]AGG Aor51HI 881 AGCGCT AGC^GCT ApaLI 685 GTGCAC G^TGCAC ApoI 988 AAATTC [GA]^AATT[CT] AspEI 67 GACTCGGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AvaI 69 CTCGGG C^[CT]CG[GA]G BamHI 1055 GGATCC G^GATCC BbeI 740 GGCGCC GGCGC^C BbsI 952 GTCTTC GAAGAC(2/6) BclI 449 TGATCA T^GATCA BglI 220 GCCACCCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BpuAI 952 GTCTTC GAAGAC(2/6) BsaI 459 GGTCTC GGTCTC(1/5) BsmAI 460 GTCTC GTCTC(1/5) BsoBI 69 CTCGGG C^[CT]CG[GA]G BspLU11I 644 ACATGT A^CATGT BsrBI 1051 GAGCGG CCGCTC(-3/-3) BsrDI 1094 GCAATG GCAATG(2/0) BstEII 588 GGTCACC G^GT[ACGT]ACC BstPI 588 GGTCACC G^GT[ACGT]ACC Bsu36I 96 CCTCAGG CC^T[ACGT]AGG CpoI 662 CGGTCCG CG^G[AT]CCG DsaI 134 CCGTGG C^C[GA][CT]GG Eam1105I 67 GACTCGGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EarI 814 CTCTTC CTCTTC(1/4) Eco47III 881 AGCGCT AGC^GCT Eco81I 96 CCTCAGG CC^T[ACGT]AGG EcoO65I 588 GGTCACC G^GT[ACGT]ACC FbaI 449 TGATCA T^GATCA KasI 740 GGCGCC G^GCGCC Ksp632I 814 CTCTTC CTCTTC(1/4) NarI 740 GGCGCC GG^CGCC PleI 67 GACTC GAGTC(4/5) PstI 745 CTGCAG CTGCA^G RsaI 177 GTAC GT^AC RsrII 662 CGGTCCG CG^G[AT]CCG SacI 562 GAGCTC GAGCT^C SfiI 219 GGCCACCCTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SfoI 740 GGCGCC GGC^GCC SphI 260 GCATGC GCATG^C Tsp509I 989 AATT ^AATT ################################### # TWO CUT ENZYMES ( 27 enzymes) # ################################### AcyI 625 GGCGTC G[GA]^CG[CT]C 740 GGCGCC AflIII 644 ACATGT A^C[GA][CT]GT 1088 ACGTGT ApaI 490 GGGCCC GGGCC^C 533 GGGCCC AspHI 562 GAGCTC G[AT]GC[AT]^C 685 GTGCAC BanI 522 GGTGCC G^G[CT][GA]CC 740 GGCGCC BpmI 355 CTGGAG CTGGAG(16/14) 975 CTGGAG BsaAI 583 TACGTG [CT]AC^GT[GA] 1087 TACGTG BsaHI 625 GGCGTC G[GA]^CG[CT]C 740 GGCGCC BsiHKAI 562 GAGCTC G[AT]GC[AT]^C 685 GTGCAC BsrFI 186 GCCGGC [GA]^CCGG[CT] 525 GCCGGC BstYI 101 GGATCT [GA]^GATC[CT] 1055 GGATCC Cfr10I 186 GCCGGC [GA]^CCGG[CT] 525 GCCGGC HapII 187 CCGG C^CGG 526 CCGG Hin1I 625 GGCGTC G[GA]^CG[CT]C 740 GGCGCC HpaII 187 CCGG C^CGG 526 CCGG MflI 101 GGATCT [GA]^GATC[CT] 1055 GGATCC MspA1I 569 CCGCTG C[AC]G^C[GT]G 748 CAGCGG MspI 187 CCGG C^CGG 526 CCGG NaeI 186 GCCGGC GCC^GGC 525 GCCGGC NgoMIV 186 GCCGGC G^CCGGC 525 GCCGGC NspI 260 GCATGC [GA]CATG^[CT] 644 ACATGT SfcI 745 CTGCAG C^T[GA][CT]AG 855 CTACAG StuI 791 AGGCCT AGG^CCT 998 AGGCCT TfiI 980 GAATC G^A[AT]TC 1067 GAATC Tsp45I 277 GTCAC ^GT[GC]AC 589 GTCAC TspRI 156 CACTG CA[GC]TG[ACGT][ACGT]^ 541 CAGTG XhoII 101 GGATCT [GA]^GATC[CT] 1055 GGATCC ######################################### # 3 OR MORE CUT ENZYMES ( 63 enzymes) # ######################################### AccI AccII AciI AluI AlwI AvaII BalI BanII BbvI BmyI BsaJI BsiYI BslI BsmFI Bsp1286I BspMI BsrI BssKI BstF5I BstNI BstUI Cac8I CfoI Cfr13I DdeI DpnII DraII EaeI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HgaI HhaI HinP1I HinfI HphI ItaI MaeII MaeIII MboI MboII MluNI MnlI MscI MslI MvaI MvnI MwoI NdeII NlaIII NlaIV Sau3AI Sau96I ScrFI SfaNI SmlI StyI TseI