(Circular) (Six-base) MAPSORT of: pFN21AE9413.seq Check: 5262 from: 1 to: 5821 pFN21AE9413 5821 bp With 182 enzymes: SgfI * December 28, 2011 15:24 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2933 2933 Size: 5821 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3631 278 Size: 53 83 186 936 2095 2468 Fragments arranged by size: 2468 2095 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2859 2973 3090 3164 1937 Size: 922 114 117 74 4594 Fragments arranged by size: 4594 922 117 114 74 Acc65I G'GTAC_C Cuts at: 3142 3142 Size: 5821 AclI AA'CG_TT Cuts at: 1563 3948 4321 1563 Size: 2385 373 3063 Fragments arranged by size: 3063 2385 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3884 5268 5536 1632 Size: 2252 1384 268 1917 Fragments arranged by size: 2252 1917 1384 268 AfeI AGC'GCT Cuts at: 2888 2888 Size: 5821 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5624 AflIII A'CryG_T Cuts at: 1203 2649 3093 4720 5697 1203 Size: 1446 444 1627 977 1327 Fragments arranged by size: 1627 1446 1327 977 444 AgeI A'CCGG_T Cuts at: 4652 4652 Size: 5821 AhdI GACnn_n'nnGTC Cuts at: 2077 4550 2077 Size: 2473 3348 AleI CACnn'nnGTG Cuts at: 1166 2600 1166 Size: 1434 4387 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3032 1184 Size: 1848 3973 AlwNI CAG_nnn'CTG Cuts at: 2925 3564 5136 2925 Size: 639 1572 3610 Fragments arranged by size: 3610 1572 639 ApaI G_GGCC'C Cuts at: 2499 2542 2499 Size: 43 5778 ApaLI G'TGCA_C Cuts at: 2690 3878 5034 2690 Size: 1188 1156 3477 Fragments arranged by size: 3477 1188 1156 ApoI r'AATT_y Cuts at: 1493 2993 3130 3356 3508 5617 1493 Size: 1500 137 226 152 2109 1697 Fragments arranged by size: 2109 1697 1500 226 152 137 AseI AT'TA_AT Cuts at: 160 1032 4375 160 Size: 872 3343 1606 Fragments arranged by size: 3343 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5821 AvaI C'yCGr_G Cuts at: 1870 1943 2074 3146 1870 Size: 73 131 1072 4545 Fragments arranged by size: 4545 1072 131 73 BamHI G'GATC_C Cuts at: 3060 3151 3518 3060 Size: 91 367 5363 Fragments arranged by size: 5363 367 91 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2527 2745 3142 4597 618 Size: 325 217 627 740 218 397 1455 1842 Fragments arranged by size: 1842 1455 740 627 397 325 218 217 BanII G_rGCy'C Cuts at: 1391 2499 2542 2571 3140 1391 Size: 1108 43 29 569 4072 Fragments arranged by size: 4072 1108 569 43 29 BbeI G_GCGC'C Cuts at: 2749 2749 Size: 5821 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2950 928 Size: 971 1051 3799 Fragments arranged by size: 3799 1051 971 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4012 4046 1903 Size: 34 2075 34 3678 Fragments arranged by size: 3678 2075 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3713 4923 3713 Size: 1210 4611 BclI T'GATC_A Cuts at: 1548 2454 1548 Size: 906 4915 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2231 4432 136 Size: 107 122 71 631 605 559 2201 1525 Fragments arranged by size: 2201 1525 631 605 559 122 107 71 BglII A'GATC_T Cuts at: 5816 5816 Size: 5821 BlpI GC'TnA_GC Cuts at: 3238 3238 Size: 5821 Bme1580I G_kGCm'C Cuts at: 1790 2499 2542 2694 3882 5038 1790 Size: 709 43 152 1188 1156 2573 Fragments arranged by size: 2573 1188 1156 709 152 43 BmgBI CAC'GTC Cuts at: 1318 2022 1318 Size: 704 5117 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2080 3552 4510 Size: 416 53 83 399 24 663 1472 958 Cuts at: 4510 442 Size: 1753 Fragments arranged by size: 1753 1472 958 663 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5821 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2381 3001 4481 1239 Size: 1142 620 1480 2579 Fragments arranged by size: 2579 1480 1142 620 Bpu10I CC'TnA_GC Cuts at: 3054 3054 Size: 5821 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2048 2800 2933 3946 4811 5109 2048 Size: 752 133 1013 865 298 2760 Fragments arranged by size: 2760 1013 865 752 298 133 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2470 4484 882 Size: 617 971 2014 2219 Fragments arranged by size: 2219 2014 971 617 BsaAI yAC'GTr Cuts at: 493 1204 2590 3094 3612 493 Size: 711 1386 504 518 2702 Fragments arranged by size: 2702 1386 711 518 504 BsaBI GATnn'nnATC Cuts at: 5815 5815 Size: 5821 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2631 2746 3628 4010 Size: 53 83 186 936 1098 115 882 382 Cuts at: 4010 4645 275 Size: 635 1451 Fragments arranged by size: 1451 1098 936 882 635 382 186 115 83 53 BsaWI w'CCGG_w Cuts at: 4254 4652 4926 5073 5484 4254 Size: 398 274 147 411 4591 Fragments arranged by size: 4591 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5596 Fragments arranged by size: 5596 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5821 BseYI C'CCAG_C Cuts at: 2554 5024 5520 2554 Size: 2470 496 2855 Fragments arranged by size: 2855 2470 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5248 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3271 4032 5060 1540 Size: 275 186 1270 761 1028 2301 Fragments arranged by size: 2301 1270 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2571 2694 3882 3967 5038 2571 Size: 123 1188 85 1071 3354 Fragments arranged by size: 3354 1188 1071 123 85 BsmI GAATG_Cn' Cuts at: 3332 3425 3332 Size: 93 5728 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2499 2542 2571 2694 3140 3882 3967 Size: 399 709 43 29 123 446 742 85 Cuts at: 3967 5038 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 709 446 399 123 85 43 29 BspEI T'CCGG_A Cuts at: 5484 5484 Size: 5821 BspHI T'CATG_A Cuts at: 3710 5440 3710 Size: 1730 4091 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2052 2223 2724 2933 3176 844 Size: 1208 171 501 209 243 3489 Fragments arranged by size: 3489 1208 501 243 209 171 BsrBI CCG'CTC Cuts at: 1394 1823 3058 3708 1394 Size: 429 1235 650 3507 Fragments arranged by size: 3507 1235 650 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3106 4316 4490 65 Size: 1183 1858 1210 174 1396 Fragments arranged by size: 1858 1396 1210 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2191 2530 4465 4652 1700 Size: 110 381 339 1935 187 2869 Fragments arranged by size: 2869 1935 381 339 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5821 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5821 BssSI C'ACGA_G Cuts at: 3881 4893 3881 Size: 1012 4809 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5821 BstBI TT'CG_AA Cuts at: 4625 4625 Size: 5821 BstEII G'GTnAC_C Cuts at: 1184 2593 1184 Size: 1409 4412 BstYI r'GATC_y Cuts at: 1972 2106 3060 3151 3518 3903 3920 5372 5458 Size: 134 954 91 367 385 17 1452 86 Cuts at: 5458 5470 5816 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 954 385 367 346 134 91 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2102 2102 Size: 5821 BtgI C'CryG_G Cuts at: 513 1065 2004 2139 513 Size: 552 939 135 4195 Fragments arranged by size: 4195 939 552 135 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2443 2473 487 Size: 683 56 1217 30 3835 Fragments arranged by size: 3835 1217 683 56 30 BtsI GCAGTG_nn' Cuts at: 3337 4131 4151 3337 Size: 794 20 5007 Fragments arranged by size: 5007 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5821 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5786 DraI TTT'AAA Cuts at: 3125 3125 Size: 5821 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4828 809 Size: 507 3512 1802 Fragments arranged by size: 3512 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2115 2205 2223 Size: 54 1425 50 275 18 285 90 18 Cuts at: 2223 2370 2607 3268 4157 5786 8 Size: 147 237 661 889 1629 43 Fragments arranged by size: 1629 1425 889 661 285 275 237 147 90 54 50 43 18 18 EagI C'GGCC_G Cuts at: 1537 1812 3268 1537 Size: 275 1456 4090 Fragments arranged by size: 4090 1456 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2825 3751 1353 Size: 1472 926 3423 Fragments arranged by size: 3423 1472 926 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4408 4782 4928 387 Size: 1064 138 2819 374 146 1280 Fragments arranged by size: 2819 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2569 2569 Size: 5821 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2381 3001 3884 4481 5268 5536 1239 Size: 393 749 620 883 597 787 268 1524 Fragments arranged by size: 1524 883 787 749 620 597 393 268 EcoO109I rG'GnC_Cy Cuts at: 2495 2496 2539 2495 Size: 1 43 5777 Fragments arranged by size: 5777 43 1 EcoRI G'AATT_C Cuts at: 3130 3508 3130 Size: 378 5443 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2974 2974 Size: 5821 FspI TGC'GCA Cuts at: 4327 4327 Size: 5821 HaeII r_GCGC'y Cuts at: 1678 2422 2749 2890 4968 1678 Size: 744 327 141 2078 2531 Fragments arranged by size: 2531 2078 744 327 141 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2063 2095 4462 4494 4536 Size: 32 135 32 491 32 2367 32 42 Cuts at: 4536 4568 1373 Size: 32 2626 Fragments arranged by size: 2626 2367 491 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3165 3411 669 Size: 988 281 1227 246 3079 Fragments arranged by size: 3079 1227 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5821 HpaI GTT'AAC Cuts at: 3411 3411 Size: 5821 KasI G'GCGC_C Cuts at: 2745 2745 Size: 5821 KpnI G_GTAC'C Cuts at: 3146 3146 Size: 5821 MfeI C'AATT_G Cuts at: 3420 3420 Size: 5821 MluI A'CGCG_T Cuts at: 5697 5697 Size: 5821 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2265 2682 4935 5119 1305 Size: 960 417 2253 184 2007 Fragments arranged by size: 2253 2007 960 417 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2117 2225 2372 10 Size: 54 1425 343 285 108 147 3459 Fragments arranged by size: 3459 1425 343 285 147 108 54 MslI CAynn'nnrTG Cuts at: 518 1166 2123 2177 2294 2390 2600 3779 4138 Size: 648 957 54 117 96 210 1179 359 Cuts at: 4138 4297 518 Size: 159 2042 Fragments arranged by size: 2042 1179 957 648 359 210 159 117 96 54 MspA1I CmG'CkG Cuts at: 1606 2576 2755 3237 3914 5062 5307 1606 Size: 970 179 482 677 1148 245 2120 Fragments arranged by size: 2120 1148 970 677 482 245 179 NaeI GCC'GGC Cuts at: 2193 2532 2193 Size: 339 5482 NarI GG'CG_CC Cuts at: 2746 2746 Size: 5821 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4330 Fragments arranged by size: 4330 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5821 NgoMIV G'CCGG_C Cuts at: 2191 2530 2191 Size: 339 5482 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5821 NotI GC'GGCC_GC Cuts at: 3268 3268 Size: 5821 NspI r_CATG'y Cuts at: 1132 1229 2269 2653 3179 4724 1132 Size: 97 1040 384 526 1545 2229 Fragments arranged by size: 2229 1545 1040 526 384 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5821 PciI A'CATG_T Cuts at: 2649 4720 2649 Size: 2071 3750 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5821 PfoI T'CCnGG_A Cuts at: 2358 2358 Size: 5821 PmeI GTTT'AAAC Cuts at: 3125 3125 Size: 5821 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3890 3922 5403 5435 3890 Size: 32 1481 32 4276 Fragments arranged by size: 4276 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5821 PsiI TTA'TAA Cuts at: 3391 3391 Size: 5821 PspOMI G'GGCC_C Cuts at: 2495 2538 2495 Size: 43 5778 PstI C_TGCA'G Cuts at: 830 2754 3173 830 Size: 1924 419 3478 Fragments arranged by size: 3478 1924 419 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5821 RsrII CG'GwC_CG Cuts at: 2668 2668 Size: 5821 SacI G_AGCT'C Cuts at: 2571 2571 Size: 5821 SalI G'TCGA_C Cuts at: 1936 3163 1936 Size: 1227 4594 SbfI CC_TGCA'GG Cuts at: 3173 3173 Size: 5821 ScaI AGT'ACT Cuts at: 4069 5781 4069 Size: 1712 4109 SfcI C'TryA_G Cuts at: 826 1045 2750 2860 3169 4304 4985 5176 826 Size: 219 1705 110 309 1135 681 191 1471 Fragments arranged by size: 1705 1471 1135 681 309 219 191 110 SfiI GGCCn_nnn'nGGCC Cuts at: 2231 2231 Size: 5821 SfoI GGC'GCC Cuts at: 2747 2747 Size: 5821 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5821 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5821 SmaI CCC'GGG Cuts at: 1872 3148 1872 Size: 1276 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2027 2779 2948 3925 4826 5088 Size: 197 926 84 752 169 977 901 262 Cuts at: 5088 820 Size: 1553 Fragments arranged by size: 1553 977 926 901 752 262 197 169 84 SnaBI TAC'GTA Cuts at: 493 3612 493 Size: 3119 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5821 SphI G_CATG'C Cuts at: 1132 2269 3179 1132 Size: 1137 910 3774 Fragments arranged by size: 3774 1137 910 SspI AAT'ATT Cuts at: 5 52 3745 5 Size: 47 3693 2081 Fragments arranged by size: 3693 2081 47 StuI AGG'CCT Cuts at: 1517 2798 3005 1517 Size: 1281 207 4333 Fragments arranged by size: 4333 1281 207 StyI C'CwwG_G Cuts at: 513 1065 2004 2112 2448 2598 2793 3260 513 Size: 552 939 108 336 150 195 467 3074 Fragments arranged by size: 3074 939 552 467 336 195 150 108 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2099 2461 3848 3865 4018 4203 4648 Size: 1255 519 362 1387 17 153 185 445 Cuts at: 4648 325 Size: 1498 Fragments arranged by size: 1498 1387 1255 519 445 362 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4067 5779 96 Size: 274 80 33 51 1443 2090 1712 138 Fragments arranged by size: 2090 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5781 5813 5781 Size: 32 5789 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5821 XbaI T'CTAG_A Cuts at: 3157 3157 Size: 5821 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5821 XmaI C'CCGG_G Cuts at: 1870 3146 1870 Size: 1276 4545 XmnI GAAnn'nnTTC Cuts at: 3950 3950 Size: 5821 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3629 276 Size: 53 83 186 936 2095 2468 Fragments arranged by size: 2468 2095 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PspOMI PstI PvuI RsrII SacI SalI SbfI ScaI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI AscI AvrII BaeI BbvCI BfrBI BplI BsiWI BsmBI BstXI BstZ17I DraIII EcoNI EcoRV FseI FspAI NruI NsiI PacI PmlI PpuMI PspXI PsrI PvuII SacII SanDI SapI SexAI SrfI SwaI XcmI