Restriction map for
pFN21AE6471
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# NO CUT ENZYMES ( 142 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcsI AcyI AflII AflIII
AgeI AhdI Alw44I AlwI AocI
ApaLI ApoI AscI AseI AsnI
Asp700I Asp718I AspEI AspI AviII
AvrII BamHI BbeI BbrPI BbvCI
BciVI BclI BfrI BglII BlnI
BlpI Bpu1102I BsaAI BsaBI BsaHI
BseAI BsiWI BsmBI BspDI BspEI
BspHI BspLU11I BssHII BssSI Bst1107I
BstBI BstEII BstPI BstUI BstXI
BstYI BstZ17I Bsu36I CelII ClaI
CpoI DpnII DraIII DrdI Eam1105I
EarI Eco81I EcoNI EcoO65I EcoRI
EcoRV FbaI FspI HgaI Hin1I
HincII HindII HindIII HpaI KasI
KpnI Ksp632I KspI MamI MboI
MfeI MflI MluI MroI MunI
MvnI NarI NdeI NdeII NheI
NotI NruI NspV PacI PaeR7I
PflFI PflMI PinAI PmaCI PmeI
PmlI PpuMI PshAI PshBI Psp1406I
PvuI RcaI RsrII SacI SacII
SalI SapI Sau3AI SbfI ScaI
SexAI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI Sse8387I SspI
StuI SwaI TfiI Tth111I Van91I
XbaI XcmI XhoI XhoII XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 33 enzymes) #
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Aor51HI 935 AGCGCT AGC^GCT
AspHI 356 GTGCTC G[AT]GC[AT]^C
AvaII 1188 GGACC G^G[AT]CC
BcgI 105 CGACTCCGGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BsiEI 493 CGGCCG CG[GA][CT]^CG
BsiHKAI 356 GTGCTC G[AT]GC[AT]^C
Bsp1407I 1034 TGTACA T^GTACA
BsrBI 61 GAGCGG CCGCTC(-3/-3)
BsrDI 698 CATTGC GCAATG(2/0)
BsrFI 1003 GCCGGC [GA]^CCGG[CT]
BsrGI 1034 TGTACA T^GTACA
BsrI 17 ACTGG ACTGG(1/-1)
BstF5I 582 GGATG GGATG(2/0)
Cfr10I 1003 GCCGGC [GA]^CCGG[CT]
DraI 433 TTTAAA TTT^AAA
EagI 493 CGGCCG C^GGCCG
EclXI 493 CGGCCG C^GGCCG
Eco47III 935 AGCGCT AGC^GCT
Eco52I 493 CGGCCG C^GGCCG
EcoT22I 342 ATGCAT ATGCA^T
FokI 582 GGATG GGATG(9/13)
FseI 1002 GGCCGGCC GGCCGG^CC
HaeII 935 AGCGCT [GA]GCGC^[CT]
MaeII 1177 ACGT A^CGT
NaeI 1003 GCCGGC GCC^GGC
NgoMIV 1003 GCCGGC G^CCGGC
NsiI 342 ATGCAT ATGCA^T
PstI 1256 CTGCAG CTGCA^G
PvuII 1195 CAGCTG CAG^CTG
SmlI 453 CTCAAG C^T[CT][GA]AG
SspBI 1034 TGTACA T^GTACA
TaqI 803 TCGA T^CGA
TthHB8I 803 TCGA T^CGA
###################################
# TWO CUT ENZYMES ( 30 enzymes) #
###################################
AluI 5 AGCT AG^CT
1196 AGCT
AlwNI 236 CAGTTGCTG CAG[ACGT][ACGT][ACGT]^CTG
1051 CAGGCACTG
ApaI 546 GGGCCC GGGCC^C
1211 GGGCCC
AvaI 36 CCCGAG C^[CT]CG[GA]G
1287 CTCGGG
BalI 884 TGGCCA TGG^CCA
1352 TGGCCA
BanI 112 GGTGCC G^G[CT][GA]CC
1225 GGCACC
BcnI 420 CCCGG CC^[GC]GG
673 CCGGG
BglI 1310 GCCCTTGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1345 GCCTTAGTGGC
BsaWI 109 TCCGGT [AT]^CCGG[AT]
526 TCCGGT
BsmI 344 GCATTC GAATGC(1/-1)
1065 GCATTC
BsoBI 36 CCCGAG C^[CT]CG[GA]G
1287 CTCGGG
BstAPI 1050 GCAGGCACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1172 GCAGGACGTGC
CfoI 873 GCGC GCG^C
936 GCGC
DdeI 352 CTCAG C^T[ACGT]AG
1347 CTTAG
FauI 125 CCCGC CCCGC(4/6)
1208 GCGGG
HhaI 873 GCGC GCG^C
936 GCGC
HinP1I 873 GCGC G^CGC
936 GCGC
HinfI 106 GACTC G^A[ACGT]TC
416 GAGTC
MluNI 884 TGGCCA TGG^CCA
1352 TGGCCA
MscI 884 TGGCCA TGG^CCA
1352 TGGCCA
MseI 434 TTAA T^TAA
1411 TTAA
MspA1I 1195 CAGCTG C[AC]G^C[GT]G
1221 CAGCGG
NciI 420 CCCGG CC^[GC]GG
673 CCGGG
NspI 340 GCATGC [GA]CATG^[CT]
986 GCATGC
PleI 106 GACTC GAGTC(4/5)
416 GAGTC
SfaNI 1181 GCATC GCATC(5/9)
1268 GCATC
SfcI 906 CTACAG C^T[GA][CT]AG
1256 CTGCAG
SphI 340 GCATGC GCATG^C
986 GCATGC
Tru9I 434 TTAA T^TAA
1411 TTAA
Tsp509I 575 AATT ^AATT
1409 AATT
#########################################
# 3 OR MORE CUT ENZYMES ( 53 enzymes) #
#########################################
AciI AfaI BanII BbsI BbvI
BfaI BmyI BpmI BpuAI BsaI
BsaJI BseRI BsgI BsiYI BslI
BsmAI BsmFI Bsp1286I BspMI BssKI
BstNI Cac8I Cfr13I DraII DsaI
EaeI EcoO109I EcoRII EcoT14I Fnu4HI
HaeIII HapII HpaII HphI ItaI
MaeI MaeIII MboII MnlI MslI
MspI MvaI MwoI NcoI NlaIII
NlaIV RsaI Sau96I ScrFI StyI
TseI Tsp45I TspRI