(Circular) (Six-base) MAPSORT of: pFN21AE6471.seq Check: 7412 from: 1 to: 6127 pFN21AE6471 6127 bp With 182 enzymes: SgfI * March 18, 2015 14:52 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3095 3095 Size: 6127 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3937 278 Size: 53 83 186 936 2401 2468 Fragments arranged by size: 2468 2401 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3470 1937 Size: 1533 4594 Acc65I G'GTAC_C Cuts at: 3448 3448 Size: 6127 AclI AA'CG_TT Cuts at: 1563 4254 4627 1563 Size: 2691 373 3063 Fragments arranged by size: 3063 2691 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 4190 5574 5842 1632 Size: 2558 1384 268 1917 Fragments arranged by size: 2558 1917 1384 268 AfeI AGC'GCT Cuts at: 2942 2942 Size: 6127 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5930 AflIII A'CryG_T Cuts at: 1203 5026 6003 1203 Size: 3823 977 1327 Fragments arranged by size: 3823 1327 977 AgeI A'CCGG_T Cuts at: 4958 4958 Size: 6127 AhdI GACnn_n'nnGTC Cuts at: 4856 4856 Size: 6127 AleI CACnn'nnGTG Cuts at: 1166 2297 2396 3393 1166 Size: 1131 99 997 3900 Fragments arranged by size: 3900 1131 997 99 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2210 3113 1184 Size: 1026 903 4198 Fragments arranged by size: 4198 1026 903 AlwNI CAG_nnn'CTG Cuts at: 2246 3061 3870 5442 2246 Size: 815 809 1572 2931 Fragments arranged by size: 2931 1572 815 809 ApaI G_GGCC'C Cuts at: 2555 3220 2555 Size: 665 5462 ApaLI G'TGCA_C Cuts at: 4184 5340 4184 Size: 1156 4971 ApoI r'AATT_y Cuts at: 1493 3436 3662 3814 5923 1493 Size: 1943 226 152 2109 1697 Fragments arranged by size: 2109 1943 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4681 160 Size: 872 3649 1606 Fragments arranged by size: 3649 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6127 AvaI C'yCGr_G Cuts at: 1870 1943 2041 3292 3452 1870 Size: 73 98 1251 160 4545 Fragments arranged by size: 4545 1251 160 98 73 BamHI G'GATC_C Cuts at: 3457 3824 3457 Size: 367 5760 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2117 3230 3448 4903 618 Size: 325 217 627 330 1113 218 1455 1842 Fragments arranged by size: 1842 1455 1113 627 330 325 218 217 BanII G_rGCy'C Cuts at: 1391 2131 2467 2555 3220 3446 1391 Size: 740 336 88 665 226 4072 Fragments arranged by size: 4072 740 665 336 226 88 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2090 2325 2332 2617 2795 928 Size: 971 191 235 7 285 178 4260 Fragments arranged by size: 4260 971 285 235 191 178 7 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2099 2133 4318 4352 1903 Size: 34 162 34 2185 34 3678 Fragments arranged by size: 3678 2185 162 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4019 5229 4019 Size: 1210 4917 BclI T'GATC_A Cuts at: 1548 1548 Size: 6127 BfrBI ATG'CAT Cuts at: 2349 2349 Size: 6127 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3321 3356 4738 Size: 107 122 71 631 605 1649 35 1382 Cuts at: 4738 136 Size: 1525 Fragments arranged by size: 1649 1525 1382 631 605 122 107 71 35 BglII A'GATC_T Cuts at: 6122 6122 Size: 6127 BlpI GC'TnA_GC Cuts at: 3544 3544 Size: 6127 Bme1580I G_kGCm'C Cuts at: 1790 2555 3220 4188 5344 1790 Size: 765 665 968 1156 2573 Fragments arranged by size: 2573 1156 968 765 665 BmgBI CAC'GTC Cuts at: 1318 3183 1318 Size: 1865 4262 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2032 3858 4816 Size: 416 53 83 399 24 615 1826 958 Cuts at: 4816 442 Size: 1753 Fragments arranged by size: 1826 1753 958 615 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6127 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2329 2954 3182 4787 1239 Size: 1090 625 228 1605 2579 Fragments arranged by size: 2579 1605 1090 625 228 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2443 4252 5117 5415 2443 Size: 1809 865 298 3155 Fragments arranged by size: 3155 1809 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2051 2056 2648 4790 882 Size: 617 552 5 592 2142 2219 Fragments arranged by size: 2219 2142 617 592 552 5 BsaAI yAC'GTr Cuts at: 493 1204 3918 493 Size: 711 2714 2702 Fragments arranged by size: 2714 2702 711 BsaBI GATnn'nnATC Cuts at: 6121 6121 Size: 6127 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3934 4316 4951 275 Size: 53 83 186 936 2401 382 635 1451 Fragments arranged by size: 2401 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2114 2531 4560 4958 5232 5379 5790 2114 Size: 417 2029 398 274 147 411 2451 Fragments arranged by size: 2451 2029 417 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2435 2465 2513 2543 1178 Size: 30 165 30 1032 30 48 30 4762 Fragments arranged by size: 4762 1032 165 48 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2251 2951 3133 3278 1186 Size: 1065 700 182 145 4035 Fragments arranged by size: 4035 1065 700 182 145 BseYI C'CCAG_C Cuts at: 2796 2995 3329 5330 5826 2796 Size: 199 334 2001 496 3097 Fragments arranged by size: 3097 2001 496 334 199 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2768 3073 3150 1163 Size: 573 1032 305 77 4140 Fragments arranged by size: 4140 1032 573 305 77 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2501 3577 4338 5366 1540 Size: 275 186 500 1076 761 1028 2301 Fragments arranged by size: 2301 1076 1028 761 500 275 186 BsiHKAI G_wGCw'C Cuts at: 2365 4188 4273 5344 2365 Size: 1823 85 1071 3148 Fragments arranged by size: 3148 1823 1071 85 BsmI GAATG_Cn' Cuts at: 2349 3070 3638 3731 2349 Size: 721 568 93 4745 Fragments arranged by size: 4745 721 568 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2131 2365 2467 2555 3220 3446 4188 Size: 399 341 234 102 88 665 226 742 Cuts at: 4188 4273 5344 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 665 399 341 234 226 102 88 85 BspEI T'CCGG_A Cuts at: 5790 5790 Size: 6127 BspHI T'CATG_A Cuts at: 4016 5746 4016 Size: 1730 4397 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2420 2850 3095 3482 844 Size: 1576 430 245 387 3489 Fragments arranged by size: 3489 1576 430 387 245 BsrBI CCG'CTC Cuts at: 1394 1823 2068 4014 1394 Size: 429 245 1946 3507 Fragments arranged by size: 3507 1946 429 245 BsrDI GCAATG_nn' Cuts at: 65 1248 2702 4622 4796 65 Size: 1183 1454 1920 174 1396 Fragments arranged by size: 1920 1454 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3008 4771 4958 1700 Size: 110 1198 1763 187 2869 Fragments arranged by size: 2869 1763 1198 187 110 BsrGI T'GTAC_A Cuts at: 96 3039 96 Size: 2943 3184 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6127 BssSI C'ACGA_G Cuts at: 4187 5199 4187 Size: 1012 5115 BstAPI GCAn_nnn'nTGC Cuts at: 794 3061 3183 794 Size: 2267 122 3738 Fragments arranged by size: 3738 2267 122 BstBI TT'CG_AA Cuts at: 4931 4931 Size: 6127 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6127 BstYI r'GATC_y Cuts at: 1972 3457 3824 4209 4226 5678 5764 5776 6122 Size: 1485 367 385 17 1452 86 12 346 Cuts at: 6122 1972 Size: 1977 Fragments arranged by size: 1977 1485 1452 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2556 2660 2892 3003 3123 513 Size: 552 939 552 104 232 111 120 3517 Fragments arranged by size: 3517 939 552 552 232 120 111 104 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2328 3262 487 Size: 683 56 1102 934 3352 Fragments arranged by size: 3352 1102 934 683 56 BtsI GCAGTG_nn' Cuts at: 3059 3092 3165 3270 3643 4437 4457 3059 Size: 33 73 105 373 794 20 4729 Fragments arranged by size: 4729 794 373 105 73 33 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6127 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 3154 3189 529 Size: 35 2590 35 3467 Fragments arranged by size: 3467 2590 35 35 DraI TTT'AAA Cuts at: 2440 3431 2440 Size: 991 5136 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5134 809 Size: 507 3818 1802 Fragments arranged by size: 3818 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2498 2889 3006 Size: 54 1425 50 275 18 668 391 117 Cuts at: 3006 3357 3574 4463 6092 8 Size: 351 217 889 1629 43 Fragments arranged by size: 1629 1425 889 668 391 351 275 217 117 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2498 3574 1537 Size: 275 686 1076 4090 Fragments arranged by size: 4090 1076 686 275 EarI CTCTTCn'nnn_ Cuts at: 1353 4057 1353 Size: 2704 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2114 4714 5088 5234 387 Size: 1064 138 525 2600 374 146 1280 Fragments arranged by size: 2600 1280 1064 525 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2329 2954 3182 4190 4787 5574 5842 Size: 393 697 625 228 1008 597 787 268 Cuts at: 5842 1239 Size: 1524 Fragments arranged by size: 1524 1008 787 697 625 597 393 268 228 EcoO109I rG'GnC_Cy Cuts at: 2552 2695 3216 2552 Size: 143 521 5463 Fragments arranged by size: 5463 521 143 EcoRI G'AATT_C Cuts at: 3436 3814 3436 Size: 378 5749 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2349 2349 Size: 6127 FseI GG_CCGG'CC Cuts at: 3012 3012 Size: 6127 FspI TGC'GCA Cuts at: 4633 4633 Size: 6127 HaeII r_GCGC'y Cuts at: 1678 2944 5274 1678 Size: 1266 2330 2531 Fragments arranged by size: 2531 2330 1266 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2018 2050 4768 4800 4842 Size: 32 135 32 446 32 2718 32 42 Cuts at: 4842 4874 1373 Size: 32 2626 Fragments arranged by size: 2718 2626 446 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3471 3717 669 Size: 988 281 1533 246 3079 Fragments arranged by size: 3079 1533 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6127 HpaI GTT'AAC Cuts at: 3717 3717 Size: 6127 KpnI G_GTAC'C Cuts at: 3452 3452 Size: 6127 MfeI C'AATT_G Cuts at: 3726 3726 Size: 6127 MluI A'CGCG_T Cuts at: 6003 6003 Size: 6127 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2104 2261 5241 5425 1305 Size: 799 157 2980 184 2007 Fragments arranged by size: 2980 2007 799 184 157 MscI TGG'CCA Cuts at: 10 64 1489 1832 2891 3359 10 Size: 54 1425 343 1059 468 2778 Fragments arranged by size: 2778 1425 1059 468 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2192 2297 2396 3393 4085 4444 4603 Size: 648 1026 105 99 997 692 359 159 Cuts at: 4603 518 Size: 2042 Fragments arranged by size: 2042 1026 997 692 648 359 159 105 99 MspA1I CmG'CkG Cuts at: 1606 3202 3228 3543 4220 5368 5613 1606 Size: 1596 26 315 677 1148 245 2120 Fragments arranged by size: 2120 1596 1148 677 315 245 26 NaeI GCC'GGC Cuts at: 3010 3010 Size: 6127 NcoI C'CATG_G Cuts at: 513 1065 2004 2660 2892 3123 513 Size: 552 939 656 232 231 3517 Fragments arranged by size: 3517 939 656 552 232 231 NdeI CA'TA_TG Cuts at: 387 387 Size: 6127 NgoMIV G'CCGG_C Cuts at: 3008 3008 Size: 6127 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6127 NotI GC'GGCC_GC Cuts at: 3574 3574 Size: 6127 NsiI A_TGCA'T Cuts at: 2351 2351 Size: 6127 NspI r_CATG'y Cuts at: 1132 1229 2349 2995 3485 5030 1132 Size: 97 1120 646 490 1545 2229 Fragments arranged by size: 2229 1545 1120 646 490 97 PasI CC'CwG_GG Cuts at: 1348 2802 3019 1348 Size: 1454 217 4456 Fragments arranged by size: 4456 1454 217 PciI A'CATG_T Cuts at: 5026 5026 Size: 6127 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6127 PmeI GTTT'AAAC Cuts at: 3431 3431 Size: 6127 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4196 4228 5709 5741 4196 Size: 32 1481 32 4582 Fragments arranged by size: 4582 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6127 PsiI TTA'TAA Cuts at: 3697 3697 Size: 6127 PspOMI G'GGCC_C Cuts at: 2551 3216 2551 Size: 665 5462 PstI C_TGCA'G Cuts at: 830 3265 3479 830 Size: 2435 214 3478 Fragments arranged by size: 3478 2435 214 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6127 PvuII CAG'CTG Cuts at: 3202 3202 Size: 6127 SalI G'TCGA_C Cuts at: 1936 3469 1936 Size: 1533 4594 SbfI CC_TGCA'GG Cuts at: 3479 3479 Size: 6127 ScaI AGT'ACT Cuts at: 4375 6087 4375 Size: 1712 4415 SfcI C'TryA_G Cuts at: 826 1045 2911 3261 3475 4610 5291 5482 826 Size: 219 1866 350 214 1135 681 191 1471 Fragments arranged by size: 1866 1471 1135 681 350 219 214 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6127 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6127 SmaI CCC'GGG Cuts at: 1872 3454 1872 Size: 1582 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2458 4231 5132 5394 820 Size: 197 926 515 1773 901 262 1553 Fragments arranged by size: 1773 1553 926 901 515 262 197 SnaBI TAC'GTA Cuts at: 493 3918 493 Size: 3425 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6127 SphI G_CATG'C Cuts at: 1132 2349 2995 3485 1132 Size: 1217 646 490 3774 Fragments arranged by size: 3774 1217 646 490 SspI AAT'ATT Cuts at: 5 52 4051 5 Size: 47 3999 2081 Fragments arranged by size: 3999 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6127 StyI C'CwwG_G Cuts at: 513 1065 2004 2224 2660 2892 2958 3111 3123 Size: 552 939 220 436 232 66 153 12 Cuts at: 3123 3566 513 Size: 443 3074 Fragments arranged by size: 3074 939 552 443 436 232 220 153 66 12 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3116 3326 4154 4171 4324 4509 4954 Size: 1255 1536 210 828 17 153 185 445 Cuts at: 4954 325 Size: 1498 Fragments arranged by size: 1536 1498 1255 828 445 210 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2684 2907 3039 Size: 274 80 33 51 1443 707 223 132 Cuts at: 3039 4373 6085 96 Size: 1334 1712 138 Fragments arranged by size: 1712 1443 1334 707 274 223 138 132 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2435 2467 6087 6119 2435 Size: 32 3620 32 2443 Fragments arranged by size: 3620 2443 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6127 XbaI T'CTAG_A Cuts at: 3463 3463 Size: 6127 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6127 XmaI C'CCGG_G Cuts at: 1870 3452 1870 Size: 1582 4545 XmnI GAAnn'nnTTC Cuts at: 4256 4256 Size: 6127 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3935 276 Size: 53 83 186 936 2401 2468 Fragments arranged by size: 2468 2401 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FseI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PspOMI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI AscI AvrII BaeI BbeI BbvCI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FspAI KasI NarI NruI PacI PfoI PmlI PpuMI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI