Restriction map for
pFN21AE6471
(Linear) (Six-base) MAPSORT of: KIEE6471.seq Check: 3623 from: 1 to: 1422
KIEE6471.orf
With 182 enzymes: SgfI *
March 16, 2015 11:32 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1090 1422
Size: 1090 332
AfeI AGC'GCT
Cuts at: 0 937 1422
Size: 937 485
AleI CACnn'nnGTG
Cuts at: 0 292 391 1388 1422
Size: 292 99 997 34
Fragments arranged by size:
997 292 99 34
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 205 1108 1422
Size: 205 903 314
Fragments arranged by size:
903 314 205
AlwNI CAG_nnn'CTG
Cuts at: 0 241 1056 1422
Size: 241 815 366
Fragments arranged by size:
815 366 241
ApaI G_GGCC'C
Cuts at: 0 550 1215 1422
Size: 550 665 207
Fragments arranged by size:
665 550 207
AvaI C'yCGr_G
Cuts at: 0 36 1287 1422
Size: 36 1251 135
Fragments arranged by size:
1251 135 36
BanI G'GyrC_C
Cuts at: 0 112 1225 1422
Size: 112 1113 197
Fragments arranged by size:
1113 197 112
BanII G_rGCy'C
Cuts at: 0 126 462 550 1215 1422
Size: 126 336 88 665 207
Fragments arranged by size:
665 336 207 126 88
BbsI GAAGACnn'nnnn_
Cuts at: 0 85 320 327 612 790 1422
Size: 85 235 7 285 178 632
Fragments arranged by size:
632 285 235 178 85 7
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 94 128 1422
Size: 94 34 1294
Fragments arranged by size:
1294 94 34
BfrBI ATG'CAT
Cuts at: 0 344 1422
Size: 344 1078
BglI GCCn_nnn'nGGC
Cuts at: 0 1316 1351 1422
Size: 1316 35 71
Fragments arranged by size:
1316 71 35
Bme1580I G_kGCm'C
Cuts at: 0 550 1215 1422
Size: 550 665 207
Fragments arranged by size:
665 550 207
BmgBI CAC'GTC
Cuts at: 0 1178 1422
Size: 1178 244
BmrI ACTGGGnnnn_n'
Cuts at: 0 27 1422
Size: 27 1395
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 324 949 1177 1422
Size: 324 625 228 245
Fragments arranged by size:
625 324 245 228
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 438 1422
Size: 438 984
BsaI GGTCTCn'nnnn_
Cuts at: 0 46 51 643 1422
Size: 46 5 592 779
Fragments arranged by size:
779 592 46 5
BsaWI w'CCGG_w
Cuts at: 0 109 526 1422
Size: 109 417 896
Fragments arranged by size:
896 417 109
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 430 460 508 538 1422
Size: 430 30 48 30 884
Fragments arranged by size:
884 430 48 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 246 946 1128 1273 1422
Size: 246 700 182 145 149
Fragments arranged by size:
700 246 182 149 145
BseYI C'CCAG_C
Cuts at: 0 791 990 1324 1422
Size: 791 199 334 98
Fragments arranged by size:
791 334 199 98
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 763 1068 1145 1422
Size: 763 305 77 277
Fragments arranged by size:
763 305 277 77
BsiEI CG_ry'CG
Cuts at: 0 496 1422
Size: 496 926
BsiHKAI G_wGCw'C
Cuts at: 0 360 1422
Size: 360 1062
BsmI GAATG_Cn'
Cuts at: 0 344 1065 1422
Size: 344 721 357
Fragments arranged by size:
721 357 344
Bsp1286I G_dGCh'C
Cuts at: 0 126 360 462 550 1215 1422
Size: 126 234 102 88 665 207
Fragments arranged by size:
665 234 207 126 102 88
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 415 845 1090 1422
Size: 415 430 245 332
Fragments arranged by size:
430 415 332 245
BsrBI CCG'CTC
Cuts at: 0 63 1422
Size: 63 1359
BsrDI GCAATG_nn'
Cuts at: 0 697 1422
Size: 697 725
BsrFI r'CCGG_y
Cuts at: 0 1003 1422
Size: 1003 419
BsrGI T'GTAC_A
Cuts at: 0 1034 1422
Size: 1034 388
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1056 1178 1422
Size: 1056 122 244
Fragments arranged by size:
1056 244 122
BtgI C'CryG_G
Cuts at: 0 551 655 887 998 1118 1422
Size: 551 104 232 111 120 304
Fragments arranged by size:
551 304 232 120 111 104
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 323 1257 1422
Size: 323 934 165
Fragments arranged by size:
934 323 165
BtsI GCAGTG_nn'
Cuts at: 0 1054 1087 1160 1265 1422
Size: 1054 33 73 105 157
Fragments arranged by size:
1054 157 105 73 33
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1149 1184 1422
Size: 1149 35 238
Fragments arranged by size:
1149 238 35
DraI TTT'AAA
Cuts at: 0 435 1422
Size: 435 987
EaeI y'GGCC_r
Cuts at: 0 493 884 1001 1352 1422
Size: 493 391 117 351 70
Fragments arranged by size:
493 391 351 117 70
EagI C'GGCC_G
Cuts at: 0 493 1422
Size: 493 929
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 109 1422
Size: 109 1313
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 324 949 1177 1422
Size: 324 625 228 245
Fragments arranged by size:
625 324 245 228
EcoO109I rG'GnC_Cy
Cuts at: 0 547 690 1211 1422
Size: 547 143 521 211
Fragments arranged by size:
547 521 211 143
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 344 1422
Size: 344 1078
FseI GG_CCGG'CC
Cuts at: 0 1007 1422
Size: 1007 415
HaeII r_GCGC'y
Cuts at: 0 939 1422
Size: 939 483
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 13 45 1422
Size: 13 32 1377
Fragments arranged by size:
1377 32 13
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 99 256 1422
Size: 99 157 1166
Fragments arranged by size:
1166 157 99
MscI TGG'CCA
Cuts at: 0 886 1354 1422
Size: 886 468 68
Fragments arranged by size:
886 468 68
MslI CAynn'nnrTG
Cuts at: 0 187 292 391 1388 1422
Size: 187 105 99 997 34
Fragments arranged by size:
997 187 105 99 34
MspA1I CmG'CkG
Cuts at: 0 1197 1223 1422
Size: 1197 26 199
Fragments arranged by size:
1197 199 26
NaeI GCC'GGC
Cuts at: 0 1005 1422
Size: 1005 417
NcoI C'CATG_G
Cuts at: 0 655 887 1118 1422
Size: 655 232 231 304
Fragments arranged by size:
655 304 232 231
NgoMIV G'CCGG_C
Cuts at: 0 1003 1422
Size: 1003 419
NsiI A_TGCA'T
Cuts at: 0 346 1422
Size: 346 1076
NspI r_CATG'y
Cuts at: 0 344 990 1422
Size: 344 646 432
Fragments arranged by size:
646 432 344
PasI CC'CwG_GG
Cuts at: 0 797 1014 1422
Size: 797 217 408
Fragments arranged by size:
797 408 217
PspOMI G'GGCC_C
Cuts at: 0 546 1211 1422
Size: 546 665 211
Fragments arranged by size:
665 546 211
PstI C_TGCA'G
Cuts at: 0 1260 1422
Size: 1260 162
PvuII CAG'CTG
Cuts at: 0 1197 1422
Size: 1197 225
SfcI C'TryA_G
Cuts at: 0 906 1256 1422
Size: 906 350 166
Fragments arranged by size:
906 350 166
SmlI C'TyrA_G
Cuts at: 0 453 1422
Size: 453 969
SphI G_CATG'C
Cuts at: 0 344 990 1422
Size: 344 646 432
Fragments arranged by size:
646 432 344
StyI C'CwwG_G
Cuts at: 0 219 655 887 953 1106 1118 1422
Size: 219 436 232 66 153 12 304
Fragments arranged by size:
436 304 232 219 153 66 12
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1111 1321 1422
Size: 1111 210 101
Fragments arranged by size:
1111 210 101
TatI w'GTAC_w
Cuts at: 0 679 902 1034 1422
Size: 679 223 132 388
Fragments arranged by size:
679 388 223 132
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 430 462 1422
Size: 430 32 960
Fragments arranged by size:
960 430 32
Enzymes that do cut:
AarI AfeI AleI AlfI AlwNI ApaI AvaI BanI
BanII BbsI BcgI BfrBI BglI Bme1580I BmgBI BmrI
BpmI BpuEI BsaI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspMI BsrBI BsrDI BsrFI
BsrGI BstAPI BtgI BtgZI BtsI CspCI DraI EaeI
EagI EciI Eco57MI EcoO109I FalI FseI HaeII Hin4I
MmeI MscI MslI MspA1I NaeI NcoI NgoMIV NsiI
NspI PasI PspOMI PstI PvuII SfcI SmlI SphI
StyI TaqII TatI TstI
Enzymes that do not cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AloI ApaLI ApoI AscI AseI AsiSI AvrII
BaeI BamHI BbeI BbvCI BciVI BclI BglII BlpI
BmtI BplI Bpu10I BsaAI BsaBI BsaHI BsiWI BsmBI
BspEI BspHI BssHII BssSI BstBI BstEII BstXI BstYI
BstZ17I Bsu36I ClaI DraIII DrdI EarI EcoICRI EcoNI
EcoRI EcoRV FspI FspAI HincII HindIII HpaI KasI
KpnI MfeI MluI NarI NdeI NheI NotI NruI
PacI PciI PflMI PfoI PmeI PmlI PpiI PpuMI
PshAI PsiI PspXI PsrI PvuI RsrII SacI SacII
SalI SanDI SapI SbfI ScaI SexAI SfiI SfoI
SgfI SgrAI SmaI SnaBI SpeI SrfI SspI StuI
SwaI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI