################################## # NO CUT ENZYMES ( 92 enzymes) # ################################## Acc65I AclI AflII AflIII AgeI AhdI Alw44I AocI Aor51HI ApaLI AscI Asp718I AspEI AvaI AviII AvrII BciVI BfrI BglII BlnI BsiEI BsiWI BsmBI BsoBI BspDI BspLU11I BsrBI BssHII Bst1107I BstBI BstEII BstPI BstZ17I Bsu36I ClaI CpoI DraI DraIII EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoO65I EcoRV EcoT22I FseI FspI HincII HindII HpaI KpnI Ksp632I KspI MfeI MluI MunI NdeI NheI NotI NruI NsiI NspV PacI PaeR7I PflMI PinAI PmeI PpuMI Psp1406I PvuI RsrII SacI SacII SalI SapI SexAI SfuI SmaI SmlI SpeI SphI SspI StuI SwaI Van91I XbaI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 55 enzymes) # ################################### AatII 2274 GACGTC GACGT^C AccI 1140 GTCTAC GT^[AC][GT]AC AccII 114 CGCG CG^CG AccIII 1256 TCCGGA T^CCGGA ApaI 79 GGGCCC GGGCC^C AseI 1246 ATTAAT AT^TAAT AsnI 1246 ATTAAT AT^TAAT Asp700I 976 GAAATGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspHI 2110 GTGCTC G[AT]GC[AT]^C AspI 1862 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC BalI 278 TGGCCA TGG^CCA BamHI 260 GGATCC G^GATCC BbrPI 229 CACGTG CAC^GTG BbvCI 594 CCTCAGC CCTCAGC(-5/-2) BcgI 47 CGATGCTGTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BclI 1521 TGATCA T^GATCA BlpI 1095 GCTGAGC GC^T[ACGT]AGC Bpu1102I 1095 GCTGAGC GC^T[ACGT]AGC BsaWI 1256 TCCGGA [AT]^CCGG[AT] BseAI 1256 TCCGGA T^CCGGA BsgI 232 GTGCAG GTGCAG(16/14) BsiHKAI 2110 GTGCTC G[AT]GC[AT]^C BsmI 586 GCATTC GAATGC(1/-1) BspEI 1256 TCCGGA T^CCGGA BspHI 1085 TCATGA T^CATGA BssSI 1159 CACGAG CACGAG(-5/-1) BstAPI 1003 GCAATCACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstUI 114 CGCG CG^CG CelII 1095 GCTGAGC GC^T[ACGT]AGC DraII 78 GGGGCCC [GA]G^G[ACGT]CC[CT] DrdI 1556 GACATTGGGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EcoNI 1233 CCTGACTCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO109I 78 GGGGCCC [GA]G^G[ACGT]CC[CT] EcoRI 537 GAATTC G^AATTC FbaI 1521 TGATCA T^GATCA MluNI 278 TGGCCA TGG^CCA MroI 1256 TCCGGA T^CCGGA MscI 278 TGGCCA TGG^CCA MvnI 114 CGCG CG^CG NcoI 125 CCATGG C^CATGG NspI 1686 ACATGC [GA]CATG^[CT] PflFI 1862 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC PmaCI 229 CACGTG CAC^GTG PmlI 229 CACGTG CAC^GTG PshAI 1236 GACTCAGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PshBI 1246 ATTAAT AT^TAAT PstI 67 CTGCAG CTGCA^G RcaI 1085 TCATGA T^CATGA SbfI 66 CCTGCAGG CCTGCA^GG SfiI 270 GGCCTCCCTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SgrAI 1915 CGCCGGCG C[GA]^CCGG[CT]G SnaBI 1036 TACGTA TAC^GTA Sse8387I 66 CCTGCAGG CCTGCA^GG Tth111I 1862 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC XmnI 976 GAAATGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 33 enzymes) # ################################### AcsI 537 GAATTC [GA]^AATT[CT] 1799 AAATTC AlwNI 616 CAGAACCTG CAG[ACGT][ACGT][ACGT]^CTG 1703 CAGAGACTG ApoI 537 GAATTC [GA]^AATT[CT] 1799 AAATTC BanII 79 GGGCCC G[GA]GC[CT]^C 1214 GAGCCC BbeI 33 GGCGCC GGCGC^C 88 GGCGCC BcnI 172 CCCGG CC^[GC]GG 2099 CCGGG BfaI 149 CTAG C^TAG 361 CTAG BglI 271 GCCTCCCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 2367 GCCACCACGGC BsaAI 229 CACGTG [CT]AC^GT[GA] 1036 TACGTA BsaI 100 GAGACC GGTCTC(1/5) 799 GAGACC Bsp1407I 758 TGTACA T^GTACA 947 TGTACA BspMI 620 ACCTGC ACCTGC(4/8) 69 GCAGGT BsrGI 758 TGTACA T^GTACA 947 TGTACA BstXI 443 CCATCGTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1691 CCATTGAGATGG BstYI 236 AGATCC [GA]^GATC[CT] 260 GGATCC EaeI 278 TGGCCA [CT]^GGCC[GA] 2217 TGGCCG EcoT14I 125 CCATGG C^C[AT][AT]GG 1898 CCAAGG HgaI 180 GACGC GACGC(5/10) 2089 GACGC KasI 33 GGCGCC G^GCGCC 88 GGCGCC MaeI 149 CTAG C^TAG 361 CTAG MflI 236 AGATCC [GA]^GATC[CT] 260 GGATCC MspA1I 644 CAGCTG C[AC]G^C[GT]G 971 CAGCTG NarI 33 GGCGCC GG^CGCC 88 GGCGCC NciI 172 CCCGG CC^[GC]GG 2099 CCGGG PvuII 644 CAGCTG CAG^CTG 971 CAGCTG ScaI 1079 AGTACT AGT^ACT 1720 AGTACT SfcI 67 CTGCAG C^T[GA][CT]AG 2287 CTACAG SfoI 33 GGCGCC GGC^GCC 88 GGCGCC SspBI 758 TGTACA T^GTACA 947 TGTACA StyI 125 CCATGG C^C[AT][AT]GG 1898 CCAAGG Tsp45I 1565 GTCAC ^GT[GC]AC 1777 GTCAC XcmI 7 CCACTGGGCAACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 2371 CCACGGCTGGTCTGG XhoII 236 AGATCC [GA]^GATC[CT] 260 GGATCC ######################################### # 3 OR MORE CUT ENZYMES ( 78 enzymes) # ######################################### AciI AcyI AfaI AluI AlwI AvaII BanI BbsI BbvI BmyI BpmI BpuAI BsaBI BsaHI BsaJI BseRI BsiYI BslI BsmAI BsmFI Bsp1286I BsrDI BsrFI BsrI BssKI BstF5I BstNI Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DsaI EcoRII FauI Fnu4HI FokI HaeII HaeIII HapII HhaI Hin1I HinP1I HindIII HinfI HpaII HphI ItaI MaeII MaeIII MamI MboI MboII MnlI MseI MslI MspI MvaI MwoI NaeI NdeII NgoMIV NlaIII NlaIV PleI RsaI Sau3AI Sau96I ScrFI SfaNI TaqI TfiI Tru9I TseI Tsp509I TspRI TthHB8I