(Circular) (Six-base) MAPSORT of: pFN21AE1997.seq Check: 6401 from: 1 to: 7141 pFN21AE1997 7141 bp With 182 enzymes: SgfI * January 31, 2011 17:47 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2065 2065 Size: 7141 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4283 4951 278 Size: 53 83 186 936 2747 668 2468 Fragments arranged by size: 2747 2468 936 668 186 83 53 AccI GT'mk_AC Cuts at: 1937 3146 4484 1937 Size: 1209 1338 4594 Fragments arranged by size: 4594 1338 1209 Acc65I G'GTAC_C Cuts at: 4462 4462 Size: 7141 AclI AA'CG_TT Cuts at: 1563 5268 5641 1563 Size: 3705 373 3063 Fragments arranged by size: 3705 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 4371 5204 6588 6856 1632 Size: 2739 833 1384 268 1917 Fragments arranged by size: 2739 1917 1384 833 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6944 AflIII A'CryG_T Cuts at: 1203 6040 7017 1203 Size: 4837 977 1327 Fragments arranged by size: 4837 1327 977 AgeI A'CCGG_T Cuts at: 5972 5972 Size: 7141 AhdI GACnn_n'nnGTC Cuts at: 5870 5870 Size: 7141 AleI CACnn'nnGTG Cuts at: 1166 2324 1166 Size: 1158 5983 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 7141 AlwNI CAG_nnn'CTG Cuts at: 2626 3713 4884 6456 2626 Size: 1087 1171 1572 3311 Fragments arranged by size: 3311 1572 1171 1087 ApaI G_GGCC'C Cuts at: 2088 2088 Size: 7141 ApaLI G'TGCA_C Cuts at: 5198 6354 5198 Size: 1156 5985 ApoI r'AATT_y Cuts at: 1493 2542 3804 4450 4676 4828 6937 1493 Size: 1049 1262 646 226 152 2109 1697 Fragments arranged by size: 2109 1697 1262 1049 646 226 152 AseI AT'TA_AT Cuts at: 160 1032 3252 5695 160 Size: 872 2220 2443 1606 Fragments arranged by size: 2443 2220 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7141 AvaI C'yCGr_G Cuts at: 1870 1943 4466 1870 Size: 73 2523 4545 Fragments arranged by size: 4545 2523 73 BamHI G'GATC_C Cuts at: 2265 4471 4838 2265 Size: 2206 367 4568 Fragments arranged by size: 4568 2206 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2038 2093 2135 3320 4271 Size: 325 217 627 251 55 42 1185 951 Cuts at: 4271 4462 5917 618 Size: 191 1455 1842 Fragments arranged by size: 1842 1455 1185 951 627 325 251 217 191 55 42 BanII G_rGCy'C Cuts at: 1391 2088 3223 4460 1391 Size: 697 1135 1237 4072 Fragments arranged by size: 4072 1237 1135 697 BbeI G_GCGC'C Cuts at: 2042 2097 2042 Size: 55 7086 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2420 3412 3542 4344 928 Size: 971 521 992 130 802 3725 Fragments arranged by size: 3725 992 971 802 521 130 BbvCI CC'TCA_GC Cuts at: 2600 2600 Size: 7141 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2041 2075 5332 5366 1903 Size: 34 104 34 3257 34 3678 Fragments arranged by size: 3678 3257 104 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 5033 6243 5033 Size: 1210 5931 BclI T'GATC_A Cuts at: 1548 3526 1548 Size: 1978 5163 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2282 4378 5752 Size: 107 122 71 631 605 610 2096 1374 Cuts at: 5752 136 Size: 1525 Fragments arranged by size: 2096 1525 1374 631 610 605 122 107 71 BglII A'GATC_T Cuts at: 7136 7136 Size: 7141 BlpI GC'TnA_GC Cuts at: 3101 4558 3101 Size: 1457 5684 Bme1580I G_kGCm'C Cuts at: 1790 2088 2138 4274 5202 6358 1790 Size: 298 50 2136 928 1156 2573 Fragments arranged by size: 2573 2136 1156 928 298 50 BmgBI CAC'GTC Cuts at: 1318 2695 3786 1318 Size: 1377 1091 4673 Fragments arranged by size: 4673 1377 1091 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2024 3184 3723 Size: 416 53 83 399 24 607 1160 539 Cuts at: 3723 4171 4872 5830 442 Size: 448 701 958 1753 Fragments arranged by size: 1753 1160 958 701 607 539 448 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7141 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2044 2603 4408 5801 1239 Size: 805 559 1805 1393 2579 Fragments arranged by size: 2579 1805 1393 805 559 Bpu10I CC'TnA_GC Cuts at: 2600 2600 Size: 7141 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 5266 6131 6429 5266 Size: 865 298 5978 Fragments arranged by size: 5978 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2099 2798 5804 882 Size: 617 600 699 3006 2219 Fragments arranged by size: 3006 2219 699 617 600 BsaAI yAC'GTr Cuts at: 493 1204 2236 3043 4932 493 Size: 711 1032 807 1889 2702 Fragments arranged by size: 2702 1889 1032 807 711 BsaBI GATnn'nnATC Cuts at: 2870 3128 3612 4006 7135 2870 Size: 258 484 394 3129 2876 Fragments arranged by size: 3129 2876 484 394 258 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2039 2094 4280 4948 Size: 53 83 186 936 506 55 2186 668 Cuts at: 4948 5330 5965 275 Size: 382 635 1451 Fragments arranged by size: 2186 1451 936 668 635 506 382 186 83 55 53 BsaWI w'CCGG_w Cuts at: 3261 5574 5972 6246 6393 6804 3261 Size: 2313 398 274 147 411 3598 Fragments arranged by size: 3598 2313 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6916 Fragments arranged by size: 6916 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2041 2210 2429 4066 1186 Size: 855 169 219 1637 4261 Fragments arranged by size: 4261 1637 855 219 169 BseYI C'CCAG_C Cuts at: 4235 6344 6840 4235 Size: 2109 496 4536 Fragments arranged by size: 4536 2109 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2258 1163 Size: 573 522 6046 Fragments arranged by size: 6046 573 522 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4591 5352 6380 1540 Size: 275 186 2590 761 1028 2301 Fragments arranged by size: 2590 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 4119 5202 5287 6358 4119 Size: 1083 85 1071 4902 Fragments arranged by size: 4902 1083 1071 85 BsmI GAATG_Cn' Cuts at: 2591 4652 4745 2591 Size: 2061 93 4987 Fragments arranged by size: 4987 2061 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2088 2138 3223 4119 4274 4460 5202 Size: 399 298 50 1085 896 155 186 742 Cuts at: 5202 5287 6358 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1085 1071 896 742 399 298 186 155 85 50 BspEI T'CCGG_A Cuts at: 3261 6804 3261 Size: 3543 3598 BspHI T'CATG_A Cuts at: 3090 5030 6760 3090 Size: 1940 1730 3471 Fragments arranged by size: 3471 1940 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2065 2634 4496 844 Size: 1221 569 1862 3489 Fragments arranged by size: 3489 1862 1221 569 BsrBI CCG'CTC Cuts at: 1394 1823 5028 1394 Size: 429 3205 3507 Fragments arranged by size: 3507 3205 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2415 3030 3297 5636 5810 65 Size: 1183 1167 615 267 2339 174 1396 Fragments arranged by size: 2339 1396 1183 1167 615 267 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2202 2462 3921 5785 5972 1700 Size: 110 392 260 1459 1864 187 2869 Fragments arranged by size: 2869 1864 1459 392 260 187 110 BsrGI T'GTAC_A Cuts at: 96 2763 2952 96 Size: 2667 189 4285 Fragments arranged by size: 4285 2667 189 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7141 BssSI C'ACGA_G Cuts at: 3164 5201 6213 3164 Size: 2037 1012 4092 Fragments arranged by size: 4092 2037 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 3014 794 Size: 2220 4921 BstBI TT'CG_AA Cuts at: 5945 5945 Size: 7141 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7141 BstXI CCAn_nnnn'nTGG Cuts at: 2455 3703 2455 Size: 1248 5893 BstYI r'GATC_y Cuts at: 1972 2241 2265 4471 4838 5223 5240 6692 6778 Size: 269 24 2206 367 385 17 1452 86 Cuts at: 6778 6790 7136 1972 Size: 12 346 1977 Fragments arranged by size: 2206 1977 1452 385 367 346 269 86 24 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2088 2130 3150 4376 513 Size: 552 939 84 42 1020 1226 3278 Fragments arranged by size: 3278 1226 1020 939 552 84 42 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6402 Fragments arranged by size: 6402 683 56 BtsI GCAGTG_nn' Cuts at: 2042 3012 3112 4657 5451 5471 2042 Size: 970 100 1545 794 20 3712 Fragments arranged by size: 3712 1545 970 794 100 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7141 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7106 DraI TTT'AAA Cuts at: 4445 4445 Size: 7141 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3567 6148 809 Size: 507 2251 2581 1802 Fragments arranged by size: 2581 2251 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2283 4222 4588 Size: 54 1425 50 275 18 453 1939 366 Cuts at: 4588 5477 7106 8 Size: 889 1629 43 Fragments arranged by size: 1939 1629 1425 889 453 366 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4588 1537 Size: 275 2776 4090 Fragments arranged by size: 4090 2776 275 EarI CTCTTCn'nnn_ Cuts at: 1353 5071 1353 Size: 3718 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2117 5728 6102 6248 387 Size: 1064 138 528 3611 374 146 1280 Fragments arranged by size: 3611 1280 1064 528 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2044 2603 4371 4408 5204 5801 6588 Size: 393 412 559 1768 37 796 597 787 Cuts at: 6588 6856 1239 Size: 268 1524 Fragments arranged by size: 1768 1524 796 787 597 559 412 393 268 37 EcoNI CCTnn'n_nnAGG Cuts at: 3242 3242 Size: 7141 EcoO109I rG'GnC_Cy Cuts at: 2084 2084 Size: 7141 EcoRI G'AATT_C Cuts at: 2542 4450 4828 2542 Size: 1908 378 4855 Fragments arranged by size: 4855 1908 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2311 2311 Size: 7141 FspI TGC'GCA Cuts at: 5647 5647 Size: 7141 HaeII r_GCGC'y Cuts at: 1678 2042 2097 4144 6288 1678 Size: 364 55 2047 2144 2531 Fragments arranged by size: 2531 2144 2047 364 55 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2381 2413 3309 3341 5782 Size: 32 135 32 809 32 896 32 2441 Cuts at: 5782 5814 5856 5888 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 2441 896 809 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 4485 4731 669 Size: 988 281 2547 246 3079 Fragments arranged by size: 3079 2547 988 281 246 HindIII A'AGCT_T Cuts at: 748 3623 3677 4028 748 Size: 2875 54 351 3861 Fragments arranged by size: 3861 2875 351 54 HpaI GTT'AAC Cuts at: 4731 4731 Size: 7141 KasI G'GCGC_C Cuts at: 2038 2093 2038 Size: 55 7086 KpnI G_GTAC'C Cuts at: 4466 4466 Size: 7141 MfeI C'AATT_G Cuts at: 4740 4740 Size: 7141 MluI A'CGCG_T Cuts at: 7017 7017 Size: 7141 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2335 6255 6439 1305 Size: 1030 3920 184 2007 Fragments arranged by size: 3920 2007 1030 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2285 10 Size: 54 1425 343 453 4866 Fragments arranged by size: 4866 1425 453 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2324 2558 2697 2813 3095 3134 3140 Size: 648 1158 234 139 116 282 39 6 Cuts at: 3140 3701 5099 5458 5617 518 Size: 561 1398 359 159 2042 Fragments arranged by size: 2042 1398 1158 648 561 359 282 234 159 139 116 39 6 MspA1I CmG'CkG Cuts at: 1606 2651 2978 4557 5234 6382 6627 1606 Size: 1045 327 1579 677 1148 245 2120 Fragments arranged by size: 2120 1579 1148 1045 677 327 245 NaeI GCC'GGC Cuts at: 2204 2464 3923 2204 Size: 260 1459 5422 Fragments arranged by size: 5422 1459 260 NarI GG'CG_CC Cuts at: 2039 2094 2039 Size: 55 7086 NcoI C'CATG_G Cuts at: 513 1065 2004 2130 513 Size: 552 939 126 5524 Fragments arranged by size: 5524 939 552 126 NdeI CA'TA_TG Cuts at: 387 387 Size: 7141 NgoMIV G'CCGG_C Cuts at: 2202 2462 3921 2202 Size: 260 1459 5422 Fragments arranged by size: 5422 1459 260 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7141 NotI GC'GGCC_GC Cuts at: 4588 4588 Size: 7141 NspI r_CATG'y Cuts at: 1132 1229 3695 4499 6044 1132 Size: 97 2466 804 1545 2229 Fragments arranged by size: 2466 2229 1545 804 97 PasI CC'CwG_GG Cuts at: 1348 3223 1348 Size: 1875 5266 PciI A'CATG_T Cuts at: 6040 6040 Size: 7141 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 7141 PfoI T'CCnGG_A Cuts at: 2176 2176 Size: 7141 PmeI GTTT'AAAC Cuts at: 4445 4445 Size: 7141 PmlI CAC'GTG Cuts at: 2236 2236 Size: 7141 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5210 5242 6723 6755 5210 Size: 32 1481 32 5596 Fragments arranged by size: 5596 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 3245 1613 Size: 1632 5509 PsiI TTA'TAA Cuts at: 2348 4711 2348 Size: 2363 4778 PspOMI G'GGCC_C Cuts at: 2084 2084 Size: 7141 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2756 2788 2756 Size: 32 7109 PstI C_TGCA'G Cuts at: 830 2076 4493 830 Size: 1246 2417 3478 Fragments arranged by size: 3478 2417 1246 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7141 PvuII CAG'CTG Cuts at: 2651 2978 2651 Size: 327 6814 SalI G'TCGA_C Cuts at: 1936 4483 1936 Size: 2547 4594 SbfI CC_TGCA'GG Cuts at: 2076 4493 2076 Size: 2417 4724 ScaI AGT'ACT Cuts at: 3086 3727 5389 7101 3086 Size: 641 1662 1712 3126 Fragments arranged by size: 3126 1712 1662 641 SfcI C'TryA_G Cuts at: 826 1045 2072 4292 4489 5624 6305 6496 826 Size: 219 1027 2220 197 1135 681 191 1471 Fragments arranged by size: 2220 1471 1135 1027 681 219 197 191 SfiI GGCCn_nnn'nGGCC Cuts at: 2282 2282 Size: 7141 SfoI GGC'GCC Cuts at: 2040 2095 2040 Size: 55 7086 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7141 SgrAI Cr'CCGG_yG Cuts at: 1700 3921 1700 Size: 2221 4920 SmaI CCC'GGG Cuts at: 1872 4468 1872 Size: 2596 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 5245 6146 6408 820 Size: 197 926 3302 901 262 1553 Fragments arranged by size: 3302 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3043 4932 493 Size: 2550 1889 2702 Fragments arranged by size: 2702 2550 1889 SpeI A'CTAG_T Cuts at: 152 152 Size: 7141 SphI G_CATG'C Cuts at: 1132 4499 1132 Size: 3367 3774 SspI AAT'ATT Cuts at: 5 52 5065 5 Size: 47 5013 2081 Fragments arranged by size: 5013 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 7141 StyI C'CwwG_G Cuts at: 513 1065 2004 2130 3903 4580 513 Size: 552 939 126 1773 677 3074 Fragments arranged by size: 3074 1773 939 677 552 126 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2461 2927 3128 3215 4289 5168 5185 Size: 1255 881 466 201 87 1074 879 17 Cuts at: 5185 5338 5523 5968 325 Size: 153 185 445 1498 Fragments arranged by size: 1498 1255 1074 881 879 466 445 201 185 153 87 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2763 2952 3084 Size: 274 80 33 51 1443 786 189 132 Cuts at: 3084 3725 5387 7099 96 Size: 641 1662 1712 138 Fragments arranged by size: 1712 1662 1443 786 641 274 189 138 132 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3968 4000 7101 7133 3968 Size: 32 3101 32 3976 Fragments arranged by size: 3976 3101 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 3870 1316 Size: 2554 4587 XbaI T'CTAG_A Cuts at: 4477 4477 Size: 7141 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2011 2019 4383 2011 Size: 8 2364 4769 Fragments arranged by size: 4769 2364 8 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7141 XmaI C'CCGG_G Cuts at: 1870 4466 1870 Size: 2596 4545 XmnI GAAnn'nnTTC Cuts at: 2985 5270 2985 Size: 2285 4856 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4281 4949 276 Size: 53 83 186 936 2747 668 2468 Fragments arranged by size: 2747 2468 936 668 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PshAI PsiI PspOMI PsrI PstI PvuI PvuII SalI SbfI ScaI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoRV FseI FspAI NruI NsiI PacI PpuMI PspXI RsrII SacI SacII SanDI SapI SexAI SrfI SwaI