Restriction map for
pFN21AE1997
(Linear) (Six-base) MAPSORT of: KIEE1997.seq Check: 3844 from: 1 to: 2436
KIEE1997.orf
With 182 enzymes: SgfI *
July 15, 2010 18:52 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 60 2436
Size: 60 2376
AatII G_ACGT'C
Cuts at: 0 2278 2436
Size: 2278 158
AccI GT'mk_AC
Cuts at: 0 1141 2436
Size: 1141 1295
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2366 2436
Size: 2366 70
AleI CACnn'nnGTG
Cuts at: 0 319 2436
Size: 319 2117
AlwNI CAG_nnn'CTG
Cuts at: 0 621 1708 2436
Size: 621 1087 728
Fragments arranged by size:
1087 728 621
ApaI G_GGCC'C
Cuts at: 0 83 2436
Size: 83 2353
ApoI r'AATT_y
Cuts at: 0 537 1799 2436
Size: 537 1262 637
Fragments arranged by size:
1262 637 537
AseI AT'TA_AT
Cuts at: 0 1247 2436
Size: 1247 1189
BamHI G'GATC_C
Cuts at: 0 260 2436
Size: 260 2176
BanI G'GyrC_C
Cuts at: 0 33 88 130 1315 2266 2436
Size: 33 55 42 1185 951 170
Fragments arranged by size:
1185 951 170 55 42 33
BanII G_rGCy'C
Cuts at: 0 83 1218 2436
Size: 83 1135 1218
Fragments arranged by size:
1218 1135 83
BbeI G_GCGC'C
Cuts at: 0 37 92 2436
Size: 37 55 2344
Fragments arranged by size:
2344 55 37
BbsI GAAGACnn'nnnn_
Cuts at: 0 415 1407 1537 2339 2436
Size: 415 992 130 802 97
Fragments arranged by size:
992 802 415 130 97
BbvCI CC'TCA_GC
Cuts at: 0 595 2436
Size: 595 1841
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 36 70 2436
Size: 36 34 2366
Fragments arranged by size:
2366 36 34
BclI T'GATC_A
Cuts at: 0 1521 2436
Size: 1521 915
BglI GCCn_nnn'nGGC
Cuts at: 0 277 2373 2436
Size: 277 2096 63
Fragments arranged by size:
2096 277 63
BlpI GC'TnA_GC
Cuts at: 0 1096 2436
Size: 1096 1340
Bme1580I G_kGCm'C
Cuts at: 0 83 133 2269 2436
Size: 83 50 2136 167
Fragments arranged by size:
2136 167 83 50
BmgBI CAC'GTC
Cuts at: 0 690 1781 2436
Size: 690 1091 655
Fragments arranged by size:
1091 690 655
BmrI ACTGGGnnnn_n'
Cuts at: 0 19 1179 1718 2166 2436
Size: 19 1160 539 448 270
Fragments arranged by size:
1160 539 448 270 19
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 39 598 2403 2436
Size: 39 559 1805 33
Fragments arranged by size:
1805 559 39 33
Bpu10I CC'TnA_GC
Cuts at: 0 595 2436
Size: 595 1841
BsaI GGTCTCn'nnnn_
Cuts at: 0 94 793 2436
Size: 94 699 1643
Fragments arranged by size:
1643 699 94
BsaAI yAC'GTr
Cuts at: 0 231 1038 2436
Size: 231 807 1398
Fragments arranged by size:
1398 807 231
BsaBI GATnn'nnATC
Cuts at: 0 865 1123 1607 2001 2436
Size: 865 258 484 394 435
Fragments arranged by size:
865 484 435 394 258
BsaHI Gr'CG_yC
Cuts at: 0 34 89 2275 2436
Size: 34 55 2186 161
Fragments arranged by size:
2186 161 55 34
BsaWI w'CCGG_w
Cuts at: 0 1256 2436
Size: 1256 1180
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 36 205 424 2061 2436
Size: 36 169 219 1637 375
Fragments arranged by size:
1637 375 219 169 36
BseYI C'CCAG_C
Cuts at: 0 2230 2436
Size: 2230 206
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 253 2436
Size: 253 2183
BsiHKAI G_wGCw'C
Cuts at: 0 2114 2436
Size: 2114 322
BsmI GAATG_Cn'
Cuts at: 0 586 2436
Size: 586 1850
Bsp1286I G_dGCh'C
Cuts at: 0 83 133 1218 2114 2269 2436
Size: 83 50 1085 896 155 167
Fragments arranged by size:
1085 896 167 155 83 50
BspEI T'CCGG_A
Cuts at: 0 1256 2436
Size: 1256 1180
BspHI T'CATG_A
Cuts at: 0 1085 2436
Size: 1085 1351
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 60 629 2436
Size: 60 569 1807
Fragments arranged by size:
1807 569 60
BsrDI GCAATG_nn'
Cuts at: 0 410 1025 1292 2436
Size: 410 615 267 1144
Fragments arranged by size:
1144 615 410 267
BsrFI r'CCGG_y
Cuts at: 0 197 457 1916 2436
Size: 197 260 1459 520
Fragments arranged by size:
1459 520 260 197
BsrGI T'GTAC_A
Cuts at: 0 758 947 2436
Size: 758 189 1489
Fragments arranged by size:
1489 758 189
BssSI C'ACGA_G
Cuts at: 0 1159 2436
Size: 1159 1277
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1009 2436
Size: 1009 1427
BstXI CCAn_nnnn'nTGG
Cuts at: 0 450 1698 2436
Size: 450 1248 738
Fragments arranged by size:
1248 738 450
BstYI r'GATC_y
Cuts at: 0 236 260 2436
Size: 236 24 2176
Fragments arranged by size:
2176 236 24
BtgI C'CryG_G
Cuts at: 0 83 125 1145 2371 2436
Size: 83 42 1020 1226 65
Fragments arranged by size:
1226 1020 83 65 42
BtsI GCAGTG_nn'
Cuts at: 0 37 1007 1107 2436
Size: 37 970 100 1329
Fragments arranged by size:
1329 970 100 37
DrdI GACnn_nn'nnGTC
Cuts at: 0 1562 2436
Size: 1562 874
EaeI y'GGCC_r
Cuts at: 0 278 2217 2436
Size: 278 1939 219
Fragments arranged by size:
1939 278 219
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 112 2436
Size: 112 2324
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 39 598 2366 2403 2436
Size: 39 559 1768 37 33
Fragments arranged by size:
1768 559 39 37 33
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1237 2436
Size: 1237 1199
EcoO109I rG'GnC_Cy
Cuts at: 0 79 2436
Size: 79 2357
EcoRI G'AATT_C
Cuts at: 0 537 2436
Size: 537 1899
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 306 2436
Size: 306 2130
HaeII r_GCGC'y
Cuts at: 0 37 92 2139 2436
Size: 37 55 2047 297
Fragments arranged by size:
2047 297 55 37
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 376 408 1304 1336 2436
Size: 376 32 896 32 1100
Fragments arranged by size:
1100 896 376 32 32
HindIII A'AGCT_T
Cuts at: 0 1618 1672 2023 2436
Size: 1618 54 351 413
Fragments arranged by size:
1618 413 351 54
KasI G'GCGC_C
Cuts at: 0 33 88 2436
Size: 33 55 2348
Fragments arranged by size:
2348 55 33
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 330 2436
Size: 330 2106
MscI TGG'CCA
Cuts at: 0 280 2436
Size: 280 2156
MslI CAynn'nnrTG
Cuts at: 0 319 553 692 808 1090 1129 1135 1696
Size: 319 234 139 116 282 39 6 561
Cuts at: 1696 2436
Size: 740
Fragments arranged by size:
740 561 319 282 234 139 116 39
6
MspA1I CmG'CkG
Cuts at: 0 646 973 2436
Size: 646 327 1463
Fragments arranged by size:
1463 646 327
NaeI GCC'GGC
Cuts at: 0 199 459 1918 2436
Size: 199 260 1459 518
Fragments arranged by size:
1459 518 260 199
NarI GG'CG_CC
Cuts at: 0 34 89 2436
Size: 34 55 2347
Fragments arranged by size:
2347 55 34
NcoI C'CATG_G
Cuts at: 0 125 2436
Size: 125 2311
NgoMIV G'CCGG_C
Cuts at: 0 197 457 1916 2436
Size: 197 260 1459 520
Fragments arranged by size:
1459 520 260 197
NspI r_CATG'y
Cuts at: 0 1690 2436
Size: 1690 746
PasI CC'CwG_GG
Cuts at: 0 1218 2436
Size: 1218 1218
PfoI T'CCnGG_A
Cuts at: 0 171 2436
Size: 171 2265
PmlI CAC'GTG
Cuts at: 0 231 2436
Size: 231 2205
PshAI GACnn'nnGTC
Cuts at: 0 1240 2436
Size: 1240 1196
PsiI TTA'TAA
Cuts at: 0 343 2436
Size: 343 2093
PspOMI G'GGCC_C
Cuts at: 0 79 2436
Size: 79 2357
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 751 783 2436
Size: 751 32 1653
Fragments arranged by size:
1653 751 32
PstI C_TGCA'G
Cuts at: 0 71 2436
Size: 71 2365
PvuII CAG'CTG
Cuts at: 0 646 973 2436
Size: 646 327 1463
Fragments arranged by size:
1463 646 327
SbfI CC_TGCA'GG
Cuts at: 0 71 2436
Size: 71 2365
ScaI AGT'ACT
Cuts at: 0 1081 1722 2436
Size: 1081 641 714
Fragments arranged by size:
1081 714 641
SfcI C'TryA_G
Cuts at: 0 67 2287 2436
Size: 67 2220 149
Fragments arranged by size:
2220 149 67
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 277 2436
Size: 277 2159
SfoI GGC'GCC
Cuts at: 0 35 90 2436
Size: 35 55 2346
Fragments arranged by size:
2346 55 35
SgrAI Cr'CCGG_yG
Cuts at: 0 1916 2436
Size: 1916 520
SnaBI TAC'GTA
Cuts at: 0 1038 2436
Size: 1038 1398
StyI C'CwwG_G
Cuts at: 0 125 1898 2436
Size: 125 1773 538
Fragments arranged by size:
1773 538 125
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 456 922 1123 1210 2284 2436
Size: 456 466 201 87 1074 152
Fragments arranged by size:
1074 466 456 201 152 87
TatI w'GTAC_w
Cuts at: 0 758 947 1079 1720 2436
Size: 758 189 132 641 716
Fragments arranged by size:
758 716 641 189 132
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1963 1995 2436
Size: 1963 32 441
Fragments arranged by size:
1963 441 32
Tth111I GACn'n_nGTC
Cuts at: 0 1865 2436
Size: 1865 571
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 14 2378 2436
Size: 14 2364 58
Fragments arranged by size:
2364 58 14
XmnI GAAnn'nnTTC
Cuts at: 0 980 2436
Size: 980 1456
ZraI GAC'GTC
Cuts at: 0 2276 2436
Size: 2276 160
Enzymes that do cut:
AarI AatII AccI AcuI AleI AlwNI ApaI ApoI
AseI BamHI BanI BanII BbeI BbsI BbvCI BcgI
BclI BglI BlpI Bme1580I BmgBI BmrI BpmI Bpu10I
BsaI BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsgI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrDI BsrFI
BsrGI BssSI BstAPI BstXI BstYI BtgI BtsI DrdI
EaeI EciI Eco57MI EcoNI EcoO109I EcoRI FalI HaeII
Hin4I HindIII KasI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NspI PasI PfoI PmlI PshAI
PsiI PspOMI PsrI PstI PvuII SbfI ScaI SfcI
SfiI SfoI SgrAI SnaBI StyI TaqII TatI TstI
Tth111I XcmI XmnI ZraI
Enzymes that do not cut:
Acc65I AclI AfeI AflII AflIII AgeI AhdI AlfI
AloI ApaLI AscI AsiSI AvaI AvrII BaeI BciVI
BfrBI BglII BmtI BplI BpuEI BsaXI BsiEI BsiWI
BsmBI BsrBI BssHII BstBI BstEII BstZ17I Bsu36I BtgZI
ClaI CspCI DraI DraIII EagI EarI EcoICRI EcoRV
FseI FspI FspAI HincII HpaI KpnI MfeI MluI
NdeI NheI NotI NruI NsiI PacI PciI PflMI
PmeI PpiI PpuMI PspXI PvuI RsrII SacI SacII
SalI SanDI SapI SexAI SgfI SmaI SmlI SpeI
SphI SrfI SspI StuI SwaI XbaI XhoI XmaI