(Linear) (Six-base) MAPSORT of: KIEE1973.seq Check: 5901 from: 1 to: 1623 KIEE1973.orf With 182 enzymes: SgfI * July 15, 2010 18:52 .. AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1513 1623 Size: 1513 110 AflIII A'CryG_T Cuts at: 0 192 1623 Size: 192 1431 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 1041 1199 1623 Size: 1041 158 424 Fragments arranged by size: 1041 424 158 ApoI r'AATT_y Cuts at: 0 400 1423 1623 Size: 400 1023 200 Fragments arranged by size: 1023 400 200 AvaI C'yCGr_G Cuts at: 0 219 1623 Size: 219 1404 BamHI G'GATC_C Cuts at: 0 771 1623 Size: 771 852 BbsI GAAGACnn'nnnn_ Cuts at: 0 969 1317 1623 Size: 969 348 306 Fragments arranged by size: 969 348 306 BclI T'GATC_A Cuts at: 0 992 1623 Size: 992 631 BfrBI ATG'CAT Cuts at: 0 1238 1623 Size: 1238 385 BmgBI CAC'GTC Cuts at: 0 73 975 1623 Size: 73 902 648 Fragments arranged by size: 902 648 73 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 508 797 1283 1623 Size: 508 289 486 340 Fragments arranged by size: 508 486 340 289 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1512 1623 Size: 1512 111 BsaWI w'CCGG_w Cuts at: 0 642 1593 1623 Size: 642 951 30 Fragments arranged by size: 951 642 30 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 1256 1259 1286 1289 1623 Size: 1256 3 27 3 334 Fragments arranged by size: 1256 334 27 3 3 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 1186 1280 1623 Size: 1186 94 343 Fragments arranged by size: 1186 343 94 BsiHKAI G_wGCw'C Cuts at: 0 1178 1623 Size: 1178 445 BsmI GAATG_Cn' Cuts at: 0 1156 1623 Size: 1156 467 BsmBI CGTCTCn'nnnn_ Cuts at: 0 1580 1623 Size: 1580 43 Bsp1286I G_dGCh'C Cuts at: 0 1178 1623 Size: 1178 445 BsrDI GCAATG_nn' Cuts at: 0 117 1623 Size: 117 1506 BssSI C'ACGA_G Cuts at: 0 123 1623 Size: 123 1500 BstXI CCAn_nnnn'nTGG Cuts at: 0 1209 1623 Size: 1209 414 BstYI r'GATC_y Cuts at: 0 280 771 1623 Size: 280 491 852 Fragments arranged by size: 852 491 280 BtsI GCAGTG_nn' Cuts at: 0 50 183 683 1623 Size: 50 133 500 940 Fragments arranged by size: 940 500 133 50 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 0 159 194 1623 Size: 159 35 1429 Fragments arranged by size: 1429 159 35 EaeI y'GGCC_r Cuts at: 0 256 899 1623 Size: 256 643 724 Fragments arranged by size: 724 643 256 EarI CTCTTCn'nnn_ Cuts at: 0 722 782 1086 1623 Size: 722 60 304 537 Fragments arranged by size: 722 537 304 60 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 508 797 1283 1513 1623 Size: 508 289 486 230 110 Fragments arranged by size: 508 486 289 230 110 EcoRV GAT'ATC Cuts at: 0 963 1623 Size: 963 660 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 265 297 977 1009 1623 Size: 265 32 680 32 614 Fragments arranged by size: 680 614 265 32 32 HincII GTy'rAC Cuts at: 0 447 1059 1623 Size: 447 612 564 Fragments arranged by size: 612 564 447 HindIII A'AGCT_T Cuts at: 0 661 927 1468 1623 Size: 661 266 541 155 Fragments arranged by size: 661 541 266 155 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 1190 1623 Size: 1190 433 MscI TGG'CCA Cuts at: 0 258 901 1623 Size: 258 643 722 Fragments arranged by size: 722 643 258 MslI CAynn'nnrTG Cuts at: 0 85 239 572 937 1623 Size: 85 154 333 365 686 Fragments arranged by size: 686 365 333 154 85 MspA1I CmG'CkG Cuts at: 0 353 1623 Size: 353 1270 NdeI CA'TA_TG Cuts at: 0 1122 1623 Size: 1122 501 NsiI A_TGCA'T Cuts at: 0 1240 1623 Size: 1240 383 NspI r_CATG'y Cuts at: 0 196 832 1623 Size: 196 636 791 Fragments arranged by size: 791 636 196 PasI CC'CwG_GG Cuts at: 0 1202 1623 Size: 1202 421 PciI A'CATG_T Cuts at: 0 192 1623 Size: 192 1431 PflMI CCAn_nnn'nTGG Cuts at: 0 164 1623 Size: 164 1459 PsiI TTA'TAA Cuts at: 0 1523 1623 Size: 1523 100 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 0 1534 1566 1623 Size: 1534 32 57 Fragments arranged by size: 1534 57 32 PstI C_TGCA'G Cuts at: 0 57 1623 Size: 57 1566 PvuII CAG'CTG Cuts at: 0 353 1623 Size: 353 1270 SfcI C'TryA_G Cuts at: 0 53 1623 Size: 53 1570 SmlI C'TyrA_G Cuts at: 0 219 1527 1623 Size: 219 1308 96 Fragments arranged by size: 1308 219 96 SpeI A'CTAG_T Cuts at: 0 376 1623 Size: 376 1247 SphI G_CATG'C Cuts at: 0 832 1623 Size: 832 791 SspI AAT'ATT Cuts at: 0 858 1623 Size: 858 765 StyI C'CwwG_G Cuts at: 0 208 1623 Size: 208 1415 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 21 1623 Size: 21 1602 TatI w'GTAC_w Cuts at: 0 64 1623 Size: 64 1559 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 821 1623 Size: 821 802 XhoI C'TCGA_G Cuts at: 0 219 1623 Size: 219 1404 Enzymes that do cut: AcuI AflIII AlfI ApoI AvaI BamHI BbsI BclI BfrBI BmgBI BpmI BpuEI BsaWI BsaXI BseRI BsiHKAI BsmI BsmBI Bsp1286I BsrDI BssSI BstXI BstYI BtsI CspCI EaeI EarI Eco57MI EcoRV Hin4I HincII HindIII MmeI MscI MslI MspA1I NdeI NsiI NspI PasI PciI PflMI PsiI PsrI PstI PvuII SfcI SmlI SpeI SphI SspI StyI TaqII TatI XcmI XhoI Enzymes that do not cut: AarI AatII AccI Acc65I AclI AfeI AflII AgeI AhdI AleI AloI AlwNI ApaI ApaLI AscI AseI AsiSI AvrII BaeI BanI BanII BbeI BbvCI BcgI BciVI BglI BglII BlpI Bme1580I BmrI BmtI BplI Bpu10I BsaI BsaAI BsaBI BsaHI BseYI BsgI BsiEI BsiWI BspEI BspHI BspMI BsrBI BsrFI BsrGI BssHII BstAPI BstBI BstEII BstZ17I Bsu36I BtgI BtgZI ClaI DraI DraIII DrdI EagI EciI EcoICRI EcoNI EcoO109I EcoRI FalI FseI FspI FspAI HaeII HpaI KasI KpnI MfeI MluI NaeI NarI NcoI NgoMIV NheI NotI NruI PacI PfoI PmeI PmlI PpiI PpuMI PshAI PspOMI PspXI PvuI RsrII SacI SacII SalI SanDI SapI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI SrfI StuI SwaI TstI Tth111I XbaI XmaI XmnI ZraI