################################## # NO CUT ENZYMES ( 146 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcyI AflII AgeI Alw44I Aor51HI ApaLI AscI AseI AsnI Asp718I AspI AviII AvrII BalI BamHI BbeI BbrPI BbsI BbvCI BcgI BciVI BfrI BglI BglII BlnI BlpI BpmI Bpu1102I BpuAI BsaBI BsaHI BsaWI BseAI BsiEI BsiWI BsmBI BsmI Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrDI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstXI BstYI BstZ17I CelII ClaI CpoI DraII DrdI DsaI EagI EclXI Eco47III Eco52I EcoNI EcoO109I EcoO65I EcoRV EcoT14I EcoT22I FseI FspI HgaI Hin1I HindIII HpaI KasI KpnI KspI MamI MfeI MflI MluI MluNI MroI MscI MunI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI PvuII RcaI RsrII SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SnaBI SpeI SphI Sse8387I SspBI StuI StyI SwaI TaqI Tth111I TthHB8I XbaI XcmI XhoI XhoII ################################### # ONE CUT ENZYMES ( 40 enzymes) # ################################### AccII 865 CGCG CG^CG AflIII 14 ACGTGT A^C[GA][CT]GT AhdI 349 GACAAAATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AlwI 937 GATCC GGATC(4/5) AocI 141 CCTGAGG CC^T[ACGT]AGG ApaI 260 GGGCCC GGGCC^C Asp700I 332 GAAATGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspEI 349 GACAAAATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AvaI 855 CCCGGG C^[CT]CG[GA]G BclI 713 TGATCA T^GATCA BsaAI 13 TACGTG [CT]AC^GT[GA] BsaI 700 GAGACC GGTCTC(1/5) BseRI 1032 CTCCTC GAGGAG(10/8) BsgI 321 CTGCAC GTGCAG(16/14) BsoBI 855 CCCGGG C^[CT]CG[GA]G BstUI 865 CGCG CG^CG Bsu36I 141 CCTGAGG CC^T[ACGT]AGG DraI 970 TTTAAA TTT^AAA DraIII 583 CACGATGTG CAC[ACGT][ACGT][ACGT]^GTG EaeI 824 CGGCCA [CT]^GGCC[GA] Eam1105I 349 GACAAAATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco81I 141 CCTGAGG CC^T[ACGT]AGG EcoRI 469 GAATTC G^AATTC FbaI 713 TGATCA T^GATCA HaeII 847 AGCGCC [GA]GCGC^[CT] HincII 432 GTCAAC GT[CT]^[GA]AC HindII 432 GTCAAC GT[CT]^[GA]AC MspA1I 615 CCGCTG C[AC]G^C[GT]G MvnI 865 CGCG CG^CG PflMI 809 CCATGCTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG SacI 364 GAGCTC GAGCT^C SfcI 134 CTGTAG C^T[GA][CT]AG SmaI 855 CCCGGG CCC^GGG SmlI 727 CTCAAG C^T[CT][GA]AG SspI 890 AATATT AAT^ATT TspRI 1073 CACTG CA[GC]TG[ACGT][ACGT]^ Van91I 809 CCATGCTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XmaCI 855 CCCGGG C^CCGGG XmaI 855 CCCGGG C^CCGGG XmnI 332 GAAATGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 27 enzymes) # ################################### AlwNI 65 CAGGGGCTG CAG[ACGT][ACGT][ACGT]^CTG 75 CAGAAGCTG AspHI 272 GTGCTC G[AT]GC[AT]^C 364 GAGCTC AvaII 231 GGTCC G^G[AT]CC 563 GGACC BanII 260 GGGCCC G[GA]GC[CT]^C 364 GAGCTC BbvI 70 GCTGC GCAGC(8/12) 80 GCTGC BcnI 855 CCCGG CC^[GC]GG 856 CCGGG BsiHKAI 272 GTGCTC G[AT]GC[AT]^C 364 GAGCTC BsiYI 768 CCCGTGTAAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 809 CCATGCTGTGG BslI 768 CCCGTGTAAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 809 CCATGCTGTGG BsmAI 247 GAGAC GTCTC(1/5) 700 GAGAC BsmFI 562 GGGAC GGGAC(10/14) 232 GTCCC BsrFI 83 GCCGGT [GA]^CCGG[CT] 790 ACCGGC BstF5I 254 GGATG GGATG(2/0) 1103 GGATG CfoI 6 GCGC GCG^C 848 GCGC Cfr10I 83 GCCGGT [GA]^CCGG[CT] 790 ACCGGC DpnII 714 GATC ^GATC 937 GATC FokI 254 GGATG GGATG(9/13) 1103 GGATG HhaI 6 GCGC GCG^C 848 GCGC HinP1I 6 GCGC G^CGC 848 GCGC HphI 41 TCACC GGTGA(8/7) 473 TCACC MboI 714 GATC ^GATC 937 GATC NciI 855 CCCGG CC^[GC]GG 856 CCGGG NdeII 714 GATC ^GATC 937 GATC PleI 217 GACTC GAGTC(4/5) 304 GACTC Sau3AI 714 GATC ^GATC 937 GATC TfiI 498 GATTC G^A[AT]TC 514 GATTC TseI 70 GCTGC G^C[AT]GC 80 GCTGC ######################################### # 3 OR MORE CUT ENZYMES ( 45 enzymes) # ######################################### AciI AcsI AfaI AluI ApoI BanI BfaI BmyI BsaJI Bsp1286I BsrI BssKI BstNI Cac8I Cfr13I DdeI EarI EcoRII FauI Fnu4HI HaeIII HapII HinfI HpaII ItaI Ksp632I MaeI MaeII MaeIII MboII MnlI MseI MslI MspI MvaI MwoI NlaIII NlaIV RsaI Sau96I ScrFI SfaNI Tru9I Tsp45I Tsp509I