(Circular) (Six-base) MAPSORT of: pFN21AE1878.seq Check: 5219 from: 1 to: 5899 pFN21AE1878 5899 bp With 182 enzymes: SgfI * August 16, 2010 14:50 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3709 278 Size: 53 83 186 936 2173 2468 Fragments arranged by size: 2468 2173 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3242 1937 Size: 1305 4594 Acc65I G'GTAC_C Cuts at: 3220 3220 Size: 5899 AclI AA'CG_TT Cuts at: 1563 4026 4399 1563 Size: 2463 373 3063 Fragments arranged by size: 3063 2463 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2036 3962 5346 5614 1632 Size: 404 1926 1384 268 1917 Fragments arranged by size: 1926 1917 1384 404 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5702 AflIII A'CryG_T Cuts at: 1203 2019 4798 5775 1203 Size: 816 2779 977 1327 Fragments arranged by size: 2779 1327 977 816 AgeI A'CCGG_T Cuts at: 4730 4730 Size: 5899 AhdI GACnn_n'nnGTC Cuts at: 2359 4628 2359 Size: 2269 3630 AleI CACnn'nnGTG Cuts at: 1166 2260 1166 Size: 1094 4805 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5899 AlwNI CAG_nnn'CTG Cuts at: 2075 2085 3642 5214 2075 Size: 10 1557 1572 2760 Fragments arranged by size: 2760 1572 1557 10 ApaI G_GGCC'C Cuts at: 2269 2269 Size: 5899 ApaLI G'TGCA_C Cuts at: 3956 5112 3956 Size: 1156 4743 ApoI r'AATT_y Cuts at: 1493 2424 2474 2549 3208 3434 3586 5695 1493 Size: 931 50 75 659 226 152 2109 1697 Fragments arranged by size: 2109 1697 931 659 226 152 75 50 AseI AT'TA_AT Cuts at: 160 1032 4453 160 Size: 872 3421 1606 Fragments arranged by size: 3421 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5899 AvaI C'yCGr_G Cuts at: 1870 1943 2860 3224 1870 Size: 73 917 364 4545 Fragments arranged by size: 4545 917 364 73 BamHI G'GATC_C Cuts at: 3229 3596 3229 Size: 367 5532 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2151 2161 2315 3220 4675 Size: 325 217 627 364 10 154 905 1455 Cuts at: 4675 618 Size: 1842 Fragments arranged by size: 1842 1455 905 627 364 325 217 154 10 BanII G_rGCy'C Cuts at: 1391 2269 2373 3218 1391 Size: 878 104 845 4072 Fragments arranged by size: 4072 878 845 104 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4928 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4090 4124 1903 Size: 34 2153 34 3678 Fragments arranged by size: 3678 2153 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3791 5001 3791 Size: 1210 4689 BclI T'GATC_A Cuts at: 1548 2718 1548 Size: 1170 4729 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4510 136 Size: 107 122 71 631 605 2838 1525 Fragments arranged by size: 2838 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5894 5894 Size: 5899 BlpI GC'TnA_GC Cuts at: 3316 3316 Size: 5899 Bme1580I G_kGCm'C Cuts at: 1790 2156 2166 2269 3960 5116 1790 Size: 366 10 103 1691 1156 2573 Fragments arranged by size: 2573 1691 1156 366 103 10 BmgBI CAC'GTC Cuts at: 1318 2497 1318 Size: 1179 4720 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3630 4588 442 Size: 416 53 83 399 24 2213 958 1753 Fragments arranged by size: 2213 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5899 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4559 1239 Size: 3320 2579 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2717 4024 4889 5187 2717 Size: 1307 865 298 3429 Fragments arranged by size: 3429 1307 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2699 4562 882 Size: 617 1200 1863 2219 Fragments arranged by size: 2219 1863 1200 617 BsaAI yAC'GTr Cuts at: 493 1204 2020 3690 493 Size: 711 816 1670 2702 Fragments arranged by size: 2702 1670 816 711 BsaBI GATnn'nnATC Cuts at: 5893 5893 Size: 5899 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3706 4088 4723 275 Size: 53 83 186 936 2173 382 635 1451 Fragments arranged by size: 2173 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4332 4730 5004 5151 5562 4332 Size: 398 274 147 411 4669 Fragments arranged by size: 4669 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2856 2886 1178 Size: 30 165 30 1453 30 4191 Fragments arranged by size: 4191 1453 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3028 1186 Size: 1842 4057 BseYI C'CCAG_C Cuts at: 5102 5598 5102 Size: 496 5403 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2311 1163 Size: 573 575 4751 Fragments arranged by size: 4751 575 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3349 4110 5138 1540 Size: 275 186 1348 761 1028 2301 Fragments arranged by size: 2301 1348 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2281 2373 3960 4045 5116 2281 Size: 92 1587 85 1071 3064 Fragments arranged by size: 3064 1587 1071 92 85 BsmI GAATG_Cn' Cuts at: 3410 3503 3410 Size: 93 5806 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2156 2166 2269 2281 2373 3218 3960 Size: 399 366 10 103 12 92 845 742 Cuts at: 3960 4045 5116 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 845 742 399 366 103 92 85 12 10 BspEI T'CCGG_A Cuts at: 5562 5562 Size: 5899 BspHI T'CATG_A Cuts at: 3788 5518 3788 Size: 1730 4169 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3254 844 Size: 2410 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3786 1394 Size: 429 1963 3507 Fragments arranged by size: 3507 1963 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4394 4568 65 Size: 1183 3146 174 1396 Fragments arranged by size: 3146 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2088 2795 4543 4730 1700 Size: 110 278 707 1748 187 2869 Fragments arranged by size: 2869 1748 707 278 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5899 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5899 BssSI C'ACGA_G Cuts at: 3959 4971 3959 Size: 1012 4887 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5899 BstBI TT'CG_AA Cuts at: 4703 4703 Size: 5899 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5899 BstYI r'GATC_y Cuts at: 1972 3229 3596 3981 3998 5450 5536 5548 5894 Size: 1257 367 385 17 1452 86 12 346 Cuts at: 5894 1972 Size: 1977 Fragments arranged by size: 1977 1452 1257 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2147 2147 Size: 5899 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 5347 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5160 Fragments arranged by size: 5160 683 56 BtsI GCAGTG_nn' Cuts at: 3415 4209 4229 3415 Size: 794 20 5085 Fragments arranged by size: 5085 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5899 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5864 DraI TTT'AAA Cuts at: 2977 3203 2977 Size: 226 5673 DraIII CAC_nnn'GTG Cuts at: 2593 2593 Size: 5899 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4906 809 Size: 507 3590 1802 Fragments arranged by size: 3590 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2829 3346 4235 Size: 54 1425 50 275 18 999 517 889 Cuts at: 4235 5864 8 Size: 1629 43 Fragments arranged by size: 1629 1425 999 889 517 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3346 1537 Size: 275 1534 4090 Fragments arranged by size: 4090 1534 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2244 2529 2697 3829 1353 Size: 891 285 168 1132 3423 Fragments arranged by size: 3423 1132 891 285 168 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4486 4860 5006 387 Size: 1064 138 2897 374 146 1280 Fragments arranged by size: 2897 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2371 2371 Size: 5899 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2036 3962 4559 5346 5614 1239 Size: 393 404 1926 597 787 268 1524 Fragments arranged by size: 1926 1524 787 597 404 393 268 EcoRI G'AATT_C Cuts at: 2474 3208 3586 2474 Size: 734 378 4787 Fragments arranged by size: 4787 734 378 FspI TGC'GCA Cuts at: 4405 4405 Size: 5899 HaeII r_GCGC'y Cuts at: 1678 2856 5046 1678 Size: 1178 2190 2531 Fragments arranged by size: 2531 2190 1178 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2345 2377 2754 2786 2926 Size: 32 135 32 773 32 377 32 140 Cuts at: 2926 2958 3170 3202 4540 4572 4614 4646 1373 Size: 32 212 32 1338 32 42 32 2626 Fragments arranged by size: 2626 1338 773 377 212 140 135 42 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2439 3243 3489 669 Size: 988 281 501 804 246 3079 Fragments arranged by size: 3079 988 804 501 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5899 HpaI GTT'AAC Cuts at: 3489 3489 Size: 5899 KpnI G_GTAC'C Cuts at: 3224 3224 Size: 5899 MfeI C'AATT_G Cuts at: 3498 3498 Size: 5899 MluI A'CGCG_T Cuts at: 5775 5775 Size: 5899 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5013 5197 1305 Size: 3708 184 2007 Fragments arranged by size: 3708 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4077 Fragments arranged by size: 4077 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2217 2260 2714 2924 3857 4216 4375 Size: 648 1051 43 454 210 933 359 159 Cuts at: 4375 518 Size: 2042 Fragments arranged by size: 2042 1051 933 648 454 359 210 159 43 MspA1I CmG'CkG Cuts at: 1606 2622 3315 3992 5140 5385 1606 Size: 1016 693 677 1148 245 2120 Fragments arranged by size: 2120 1148 1016 693 677 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5347 NdeI CA'TA_TG Cuts at: 387 387 Size: 5899 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5899 NotI GC'GGCC_GC Cuts at: 3346 3346 Size: 5899 NspI r_CATG'y Cuts at: 1132 1229 3257 4802 1132 Size: 97 2028 1545 2229 Fragments arranged by size: 2229 2028 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5899 PciI A'CATG_T Cuts at: 4798 4798 Size: 5899 PflMI CCAn_nnn'nTGG Cuts at: 1108 2820 1108 Size: 1712 4187 PmeI GTTT'AAAC Cuts at: 3203 3203 Size: 5899 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3968 4000 5481 5513 3968 Size: 32 1481 32 4354 Fragments arranged by size: 4354 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5899 PsiI TTA'TAA Cuts at: 3469 3469 Size: 5899 PspOMI G'GGCC_C Cuts at: 2265 2265 Size: 5899 PstI C_TGCA'G Cuts at: 830 3251 830 Size: 2421 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5899 SacI G_AGCT'C Cuts at: 2373 2373 Size: 5899 SalI G'TCGA_C Cuts at: 1936 3241 1936 Size: 1305 4594 SbfI CC_TGCA'GG Cuts at: 3251 3251 Size: 5899 ScaI AGT'ACT Cuts at: 4147 5859 4147 Size: 1712 4187 SfcI C'TryA_G Cuts at: 826 1045 2139 3247 4382 5063 5254 826 Size: 219 1094 1108 1135 681 191 1471 Fragments arranged by size: 1471 1135 1108 1094 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5899 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5899 SmaI CCC'GGG Cuts at: 1872 2862 3226 1872 Size: 990 364 4545 Fragments arranged by size: 4545 990 364 SmlI C'TyrA_G Cuts at: 820 1017 1943 2732 4003 4904 5166 820 Size: 197 926 789 1271 901 262 1553 Fragments arranged by size: 1553 1271 926 901 789 262 197 SnaBI TAC'GTA Cuts at: 493 3690 493 Size: 3197 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5899 SphI G_CATG'C Cuts at: 1132 3257 1132 Size: 2125 3774 SspI AAT'ATT Cuts at: 5 52 2897 3823 5 Size: 47 2845 926 2081 Fragments arranged by size: 2845 2081 926 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5899 StyI C'CwwG_G Cuts at: 513 1065 3195 3338 513 Size: 552 2130 143 3074 Fragments arranged by size: 3074 2130 552 143 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2855 2891 3926 3943 4096 4281 4726 Size: 1255 1275 36 1035 17 153 185 445 Cuts at: 4726 325 Size: 1498 Fragments arranged by size: 1498 1275 1255 1035 445 185 153 36 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4145 5857 96 Size: 274 80 33 51 1443 2168 1712 138 Fragments arranged by size: 2168 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2856 2888 5859 5891 2856 Size: 32 2971 32 2864 Fragments arranged by size: 2971 2864 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5899 XbaI T'CTAG_A Cuts at: 3235 3235 Size: 5899 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5899 XmaI C'CCGG_G Cuts at: 1870 2860 3224 1870 Size: 990 364 4545 Fragments arranged by size: 4545 990 364 XmnI GAAnn'nnTTC Cuts at: 2341 4028 2341 Size: 1687 4212 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3707 276 Size: 53 83 186 936 2173 2468 Fragments arranged by size: 2468 2173 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PspOMI PstI PvuI SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I EcoNI EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspXI PsrI PvuII RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI