################################## # NO CUT ENZYMES ( 200 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcyI AflII AflIII AgeI AhdI Alw44I AlwI AlwNI AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AspI AvaI AviII AvrII BalI BamHI BanI BanII BbeI BbrPI BbvCI BcgI BclI BcnI BfaI BfrI BglI BglII BlnI BlpI BmyI BpmI Bpu1102I BsaAI BsaHI BsaI BsaWI BseAI BseRI BsiEI BsiHKAI BsiWI BsmBI BsmFI BsmI BsoBI Bsp1286I Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstXI BstYI BstZ17I Bsu36I Cac8I CelII CfoI Cfr10I ClaI CpoI DpnII DraI DraII DraIII DrdI DsaI EaeI EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRV EcoT14I EcoT22I FauI FbaI FseI FspI HaeII HgaI HhaI Hin1I HinP1I HincII HindII HpaI KasI KpnI Ksp632I KspI MaeI MaeII MboI MfeI MflI MluI MluNI MroI MscI MspA1I MunI MvnI NaeI NarI NciI NcoI NdeI NdeII NgoMIV NheI NlaIV NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI PvuII RcaI RsrII SacI SacII SalI SapI Sau3AI SbfI SexAI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI StyI SwaI Tsp45I Tth111I Van91I XbaI XcmI XhoI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 23 enzymes) # ################################### BbsI 377 GTCTTC GAAGAC(2/6) BbvI 145 GCTGC GCAGC(8/12) BpuAI 377 GTCTTC GAAGAC(2/6) BsaBI 472 GATGTGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsgI 31 GTGCAG GTGCAG(16/14) BsiYI 717 CCTGGCACTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BslI 717 CCTGGCACTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BsrDI 644 CATTGC GCAATG(2/0) HaeIII 231 GGCC GG^CC HapII 80 CCGG C^CGG HindIII 334 AAGCTT A^AGCTT HpaII 80 CCGG C^CGG HphI 737 TCACC GGTGA(8/7) MamI 472 GATGTGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MnlI 691 CCTC CCTC(7/6) MspI 80 CCGG C^CGG MwoI 702 GCTCTACGGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC PleI 44 GAGTC GAGTC(4/5) ScaI 10 AGTACT AGT^ACT SfcI 287 CTATAG C^T[GA][CT]AG TaqI 207 TCGA T^CGA TseI 145 GCTGC G^C[AT]GC TthHB8I 207 TCGA T^CGA ################################### # TWO CUT ENZYMES ( 17 enzymes) # ################################### AciI 117 CCGC CCGC(-3/-1) 64 GCGG AluI 335 AGCT AG^CT 524 AGCT AvaII 222 GGACC G^G[AT]CC 255 GGTCC BciVI 634 GGATAC GTATCC(6/5) 678 GTATCC BsmAI 325 GAGAC GTCTC(1/5) 440 GAGAC BsrI 723 ACTGG ACTGG(1/-1) 225 CCAGT DdeI 27 CTTAG C^T[ACGT]AG 103 CTGAG EcoRI 21 GAATTC G^AATTC 51 GAATTC Fnu4HI 64 GCGGC GC^[ACGT]GC 145 GCTGC ItaI 64 GCGGC GC^[ACGT]GC 145 GCTGC MaeIII 3 GTTAC ^GT[ACGT]AC 622 GTAAC MboII 378 TCTTC GAAGA(8/7) 672 TCTTC MseI 262 TTAA T^TAA 394 TTAA MslI 478 CATCTCAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 722 CACTGGGATG NlaIII 312 CATG CATG^ 541 CATG TfiI 317 GATTC G^A[AT]TC 696 GATTC Tru9I 262 TTAA T^TAA 394 TTAA ######################################### # 3 OR MORE CUT ENZYMES ( 18 enzymes) # ######################################### AcsI AfaI ApoI BsaJI BssKI BstF5I BstNI Cfr13I EcoRII FokI HinfI MvaI RsaI Sau96I ScrFI SfaNI Tsp509I TspRI