################################## # NO CUT ENZYMES ( 101 enzymes) # ################################## AatII Acc65I AccIII AclI AflIII AgeI AhdI AocI Aor51HI ApaI AscI AseI AsnI Asp700I Asp718I AspEI AvrII BamHI BbrPI BciVI BclI BfaI BlnI BlpI Bpu1102I BsaAI BseAI BsgI BsiWI BspEI BspHI BspLU11I BsrBI BssHII BssSI Bst1107I BstBI BstZ17I Bsu36I CelII CpoI DraI DraIII EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoRV EcoT22I FbaI FseI HincII HindII HindIII HpaI KpnI Ksp632I KspI MaeI MfeI MluI MroI MunI NaeI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I RcaI RsrII SacII SalI SapI SbfI ScaI SfiI SfuI SgrAI SnaBI SpeI SphI Sse8387I SspI StuI SwaI XbaI XmnI ################################### # ONE CUT ENZYMES ( 42 enzymes) # ################################### AcsI 856 GAATTC [GA]^AATT[CT] AcyI 1037 GGCGCC G[GA]^CG[CT]C AflII 319 CTTAAG C^TTAAG ApoI 856 GAATTC [GA]^AATT[CT] AspI 123 GACATCGTC GAC[ACGT]^[ACGT][ACGT]GTC AviII 239 TGCGCA TGC^GCA BanI 1037 GGCGCC G^G[CT][GA]CC BbeI 1037 GGCGCC GGCGC^C BcgI 1631 GCAGCTACTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BfrI 319 CTTAAG C^TTAAG BglII 69 AGATCT A^GATCT BsaHI 1037 GGCGCC G[GA]^CG[CT]C BsiEI 473 CGATCG CG[GA][CT]^CG BspDI 1834 ATCGAT AT^CGAT BspMI 242 GCAGGT ACCTGC(4/8) BsrFI 196 ACCGGC [GA]^CCGG[CT] BstAPI 1263 GCAGTACATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC Cfr10I 196 ACCGGC [GA]^CCGG[CT] ClaI 1834 ATCGAT AT^CGAT DrdI 997 GACTCCTGGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EcoNI 1734 CCTCTCAAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoRI 856 GAATTC G^AATTC FspI 239 TGCGCA TGC^GCA Hin1I 1037 GGCGCC G[GA]^CG[CT]C KasI 1037 GGCGCC G^GCGCC MaeII 1412 ACGT A^CGT NarI 1037 GGCGCC GG^CGCC NcoI 152 CCATGG C^CATGG PaeR7I 679 CTCGAG C^TCGAG PflFI 123 GACATCGTC GAC[ACGT]^[ACGT][ACGT]GTC PvuI 473 CGATCG CGAT^CG PvuII 1894 CAGCTG CAG^CTG SacI 662 GAGCTC GAGCT^C SexAI 1924 ACCAGGT A^CC[AT]GGT SfoI 1037 GGCGCC GGC^GCC SmaI 2047 CCCGGG CCC^GGG TfiI 1252 GATTC G^A[AT]TC Tsp509I 857 AATT ^AATT Tth111I 123 GACATCGTC GAC[ACGT]^[ACGT][ACGT]GTC XhoI 679 CTCGAG C^TCGAG XmaCI 2047 CCCGGG C^CCGGG XmaI 2047 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 30 enzymes) # ################################### AccI 1006 GTCTAC GT^[AC][GT]AC 1147 GTAGAC Alw44I 262 GTGCAC G^TGCAC 1414 GTGCAC AlwNI 564 CAGGGCCTG CAG[ACGT][ACGT][ACGT]^CTG 1810 CAGATCCTG ApaLI 262 GTGCAC G^TGCAC 1414 GTGCAC AvaI 679 CTCGAG C^[CT]CG[GA]G 2047 CCCGGG BbvCI 93 CCTCAGC CCTCAGC(-5/-2) 1620 CCTCAGC BsaI 157 GAGACC GGTCTC(1/5) 1102 GAGACC BsaWI 282 ACCGGA [AT]^CCGG[AT] 1313 TCCGGT BsmBI 1981 CGTCTC CGTCTC(1/5) 1300 GAGACG BsoBI 679 CTCGAG C^[CT]CG[GA]G 2047 CCCGGG Bsp1407I 923 TGTACA T^GTACA 1556 TGTACA BsrDI 344 GCAATG GCAATG(2/0) 1190 GCAATG BsrGI 923 TGTACA T^GTACA 1556 TGTACA BstEII 1284 GGTCACC G^GT[ACGT]ACC 1602 GGTCACC BstPI 1284 GGTCACC G^GT[ACGT]ACC 1602 GGTCACC BstYI 69 AGATCT [GA]^GATC[CT] 1811 AGATCC EcoO65I 1284 GGTCACC G^GT[ACGT]ACC 1602 GGTCACC FauI 1305 GCGGG CCCGC(4/6) 1855 GCGGG HaeII 394 AGCGCC [GA]GCGC^[CT] 1037 GGCGCC HgaI 1302 GACGC GACGC(5/10) 1980 GCGTC MaeIII 1285 GTCAC ^GT[ACGT]AC 1603 GTCAC MflI 69 AGATCT [GA]^GATC[CT] 1811 AGATCC MspA1I 507 CCGCTG C[AC]G^C[GT]G 1894 CAGCTG NspI 258 GCATGT [GA]CATG^[CT] 1268 ACATGC PflMI 1027 CCAGAGCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1608 CCAGTTCGTGG SspBI 923 TGTACA T^GTACA 1556 TGTACA Tsp45I 1285 GTCAC ^GT[GC]AC 1603 GTCAC Van91I 1027 CCAGAGCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1608 CCAGTTCGTGG XcmI 1781 CCAAGAAGGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 2001 CCAGGTGAAGGGTGG XhoII 69 AGATCT [GA]^GATC[CT] 1811 AGATCC ######################################### # 3 OR MORE CUT ENZYMES ( 85 enzymes) # ######################################### AccII AciI AfaI AluI AlwI AspHI AvaII BalI BanII BbsI BbvI BcnI BglI BmyI BpmI BpuAI BsaBI BsaJI BseRI BsiHKAI BsiYI BslI BsmAI BsmFI BsmI Bsp1286I BsrI BssKI BstF5I BstNI BstUI BstXI Cac8I CfoI Cfr13I DdeI DpnII DraII DsaI EaeI EcoO109I EcoRII EcoT14I Fnu4HI FokI HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI MamI MboI MboII MluNI MnlI MscI MseI MslI MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV PleI PpuMI PstI RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI StyI TaqI Tru9I TseI TspRI TthHB8I