Restriction map for
pFN21AE5752
(Circular) (Six-base) MAPSORT of: pFN21AE5752.seq Check: 5757 from: 1 to: 6787
pFN21AE5752 6787 bp
With 182 enzymes: SgfI *
December 8, 2014 13:43 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2238 2238
Size: 6787
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4597 278
Size: 53 83 186 936 3061 2468
Fragments arranged by size:
3061 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3012 3153 4130 1937
Size: 1075 141 977 4594
Fragments arranged by size:
4594 1075 977 141
Acc65I G'GTAC_C
Cuts at: 4108 4108
Size: 6787
AclI AA'CG_TT
Cuts at: 1563 4914 5287 1563
Size: 3351 373 3063
Fragments arranged by size:
3351 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2861 3416 3455 3512 4850 6234 6502 1632
Size: 1229 555 39 57 1338 1384 268 1917
Fragments arranged by size:
1917 1384 1338 1229 555 268 57 39
AflII C'TTAA_G
Cuts at: 820 1017 2324 820
Size: 197 1307 5283
Fragments arranged by size:
5283 1307 197
AflIII A'CryG_T
Cuts at: 1203 5686 6663 1203
Size: 4483 977 1327
Fragments arranged by size:
4483 1327 977
AgeI A'CCGG_T
Cuts at: 5618 5618
Size: 6787
AhdI GACnn_n'nnGTC
Cuts at: 5516 5516
Size: 6787
AleI CACnn'nnGTG
Cuts at: 1166 3188 1166
Size: 2022 4765
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6787
AlwNI CAG_nnn'CTG
Cuts at: 2574 3820 4530 6102 2574
Size: 1246 710 1572 3259
Fragments arranged by size:
3259 1572 1246 710
ApaLI G'TGCA_C
Cuts at: 2267 3419 4844 6000 2267
Size: 1152 1425 1156 3054
Fragments arranged by size:
3054 1425 1156 1152
ApoI r'AATT_y
Cuts at: 1493 2861 4096 4322 4474 6583 1493
Size: 1368 1235 226 152 2109 1697
Fragments arranged by size:
2109 1697 1368 1235 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5341 160
Size: 872 4309 1606
Fragments arranged by size:
4309 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6787
AvaI C'yCGr_G
Cuts at: 1870 1943 2684 4052 4112 1870
Size: 73 741 1368 60 4545
Fragments arranged by size:
4545 1368 741 73 60
BamHI G'GATC_C
Cuts at: 4117 4484 4117
Size: 367 6420
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3042 4108 5563 618
Size: 325 217 627 1255 1066 1455 1842
Fragments arranged by size:
1842 1455 1255 1066 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2110 2195 2671 3206 4106 1391
Size: 719 85 476 535 900 4072
Fragments arranged by size:
4072 900 719 535 476 85
BbeI G_GCGC'C
Cuts at: 3046 3046
Size: 6787
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2465 2533 3356 928
Size: 971 566 68 823 4359
Fragments arranged by size:
4359 971 823 566 68
BbvCI CC'TCA_GC
Cuts at: 2099 3626 2099
Size: 1527 5260
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3625 3659 4978 5012 1903
Size: 34 1688 34 1319 34 3678
Fragments arranged by size:
3678 1688 1319 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4679 5889 4679
Size: 1210 5577
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6787
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3233 3593 3791
Size: 107 122 71 631 605 1561 360 198
Cuts at: 3791 5398 136
Size: 1607 1525
Fragments arranged by size:
1607 1561 1525 631 605 360 198 122
107 71
BglII A'GATC_T
Cuts at: 2074 6782 2074
Size: 4708 2079
BlpI GC'TnA_GC
Cuts at: 4204 4204
Size: 6787
Bme1580I G_kGCm'C
Cuts at: 1790 2271 2807 3423 4848 6004 1790
Size: 481 536 616 1425 1156 2573
Fragments arranged by size:
2573 1425 1156 616 536 481
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6787
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3607 4518 5476
Size: 416 53 83 399 24 2190 911 958
Cuts at: 5476 442
Size: 1753
Fragments arranged by size:
2190 1753 958 911 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6787
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2023 3034 3818 5447 1239
Size: 784 1011 784 1629 2579
Fragments arranged by size:
2579 1629 1011 784 784
Bpu10I CC'TnA_GC
Cuts at: 2099 3626 2099
Size: 1527 5260
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2036 3359 3902 4912 5777 6075 2036
Size: 1323 543 1010 865 298 2748
Fragments arranged by size:
2748 1323 1010 865 543 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2156 3101 5450 882
Size: 617 657 945 2349 2219
Fragments arranged by size:
2349 2219 945 657 617
BsaAI yAC'GTr
Cuts at: 493 1204 4578 493
Size: 711 3374 2702
Fragments arranged by size:
3374 2702 711
BsaBI GATnn'nnATC
Cuts at: 2079 3410 3563 6781 2079
Size: 1331 153 3218 2085
Fragments arranged by size:
3218 2085 1331 153
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3043 4594 4976 5611
Size: 53 83 186 936 1510 1551 382 635
Cuts at: 5611 275
Size: 1451
Fragments arranged by size:
1551 1510 1451 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2287 3318 5220 5618 5892 6039 6450 2287
Size: 1031 1902 398 274 147 411 2624
Fragments arranged by size:
2624 1902 1031 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2117 2147 1178
Size: 30 165 30 714 30 5818
Fragments arranged by size:
5818 714 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2138 2462 2465 3146 3815 3860 3899 1186
Size: 952 324 3 681 669 45 39 4074
Fragments arranged by size:
4074 952 681 669 324 45 39 3
BseYI C'CCAG_C
Cuts at: 3901 5990 6486 3901
Size: 2089 496 4202
Fragments arranged by size:
4202 2089 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 6214
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2481 4237 4998 6026 1540
Size: 275 186 480 1756 761 1028 2301
Fragments arranged by size:
2301 1756 1028 761 480 275 186
BsiHKAI G_wGCw'C
Cuts at: 2271 2671 3375 3423 4848 4933 6004 2271
Size: 400 704 48 1425 85 1071 3054
Fragments arranged by size:
3054 1425 1071 704 400 85 48
BsmI GAATG_Cn'
Cuts at: 2959 3229 3657 4298 4391 2959
Size: 270 428 641 93 5355
Fragments arranged by size:
5355 641 428 270 93
BsmBI CGTCTCn'nnnn_
Cuts at: 3299 3992 3299
Size: 693 6094
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2110 2195 2271 2671 2807 3206 3375
Size: 399 320 85 76 400 136 399 169
Cuts at: 3375 3423 4106 4848 4933 6004 1391
Size: 48 683 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 683 400 399 399 320
169 136 85 85 76 48
BspEI T'CCGG_A
Cuts at: 6450 6450
Size: 6787
BspHI T'CATG_A
Cuts at: 4676 6406 4676
Size: 1730 5057
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2238 4142 844
Size: 1394 1904 3489
Fragments arranged by size:
3489 1904 1394
BsrBI CCG'CTC
Cuts at: 1394 1823 4674 1394
Size: 429 2851 3507
Fragments arranged by size:
3507 2851 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2356 3202 5282 5456 65
Size: 1183 1108 846 2080 174 1396
Fragments arranged by size:
2080 1396 1183 1108 846 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2201 5431 5618 1700
Size: 110 391 3230 187 2869
Fragments arranged by size:
3230 2869 391 187 110
BsrGI T'GTAC_A
Cuts at: 96 2928 3561 96
Size: 2832 633 3322
Fragments arranged by size:
3322 2832 633
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6787
BssSI C'ACGA_G
Cuts at: 4847 5859 4847
Size: 1012 5775
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3274 794
Size: 2480 4307
BstBI TT'CG_AA
Cuts at: 5591 5591
Size: 6787
BstEII G'GTnAC_C
Cuts at: 1184 3289 3607 1184
Size: 2105 318 4364
Fragments arranged by size:
4364 2105 318
BstXI CCAn_nnnn'nTGG
Cuts at: 2173 2785 3190 3301 2173
Size: 612 405 111 5659
Fragments arranged by size:
5659 612 405 111
BstYI r'GATC_y
Cuts at: 1972 2074 3816 4117 4484 4869 4886 6338 6424
Size: 102 1742 301 367 385 17 1452 86
Cuts at: 6424 6436 6782 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1742 1452 385 367 346 301 102
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2157 2311 3588 513
Size: 552 939 153 154 1277 3712
Fragments arranged by size:
3712 1277 939 552 154 153
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2512 3645 487
Size: 683 56 1286 1133 3629
Fragments arranged by size:
3629 1286 1133 683 56
BtsI GCAGTG_nn'
Cuts at: 4303 5097 5117 4303
Size: 794 20 5973
Fragments arranged by size:
5973 794 20
ClaI AT'CG_AT
Cuts at: 1554 3840 1554
Size: 2286 4501
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6752
DraI TTT'AAA
Cuts at: 4091 4091
Size: 6787
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3008 5794 809
Size: 507 1692 2786 1802
Fragments arranged by size:
2786 1802 1692 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2007 2203 2412
Size: 54 1425 50 275 18 177 196 209
Cuts at: 2412 3585 3699 3852 4234 5123 6752 8
Size: 1173 114 153 382 889 1629 43
Fragments arranged by size:
1629 1425 1173 889 382 275 209 196
177 153 114 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4234 1537
Size: 275 2422 4090
Fragments arranged by size:
4090 2422 275
EarI CTCTTCn'nnn_
Cuts at: 1353 4717 1353
Size: 3364 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5374 5748 5894 387
Size: 1064 138 3785 374 146 1280
Fragments arranged by size:
3785 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2669 2669
Size: 6787
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2023 2861 3034 3416 3455 3512 3818
Size: 393 391 838 173 382 39 57 306
Cuts at: 3818 4850 5447 6234 6502 1239
Size: 1032 597 787 268 1524
Fragments arranged by size:
1524 1032 838 787 597 393 391 382
306 268 173 57 39
EcoNI CCTnn'n_nnAGG
Cuts at: 3743 3743
Size: 6787
EcoO109I rG'GnC_Cy
Cuts at: 2043 2571 3763 3793 3877 3906 2043
Size: 528 1192 30 84 29 4924
Fragments arranged by size:
4924 1192 528 84 30 29
EcoRI G'AATT_C
Cuts at: 2861 4096 4474 2861
Size: 1235 378 5174
Fragments arranged by size:
5174 1235 378
FspI TGC'GCA
Cuts at: 2246 5293 2246
Size: 3047 3740
HaeII r_GCGC'y
Cuts at: 1678 2403 3046 5934 1678
Size: 725 643 2888 2531
Fragments arranged by size:
2888 2531 725 643
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2114 2117 2146 2149 2576
Size: 32 135 32 542 3 29 3 427
Cuts at: 2576 2608 2750 2782 3801 3833 5428 5460 5502
Size: 32 142 32 1019 32 1595 32 42
Cuts at: 5502 5534 1373
Size: 32 2626
Fragments arranged by size:
2626 1595 1019 542 427 142 135 42
32 32 32 32 32 32 32 29
3 3
HincII GTy'rAC
Cuts at: 669 1657 1938 4131 4377 669
Size: 988 281 2193 246 3079
Fragments arranged by size:
3079 2193 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6787
HpaI GTT'AAC
Cuts at: 4377 4377
Size: 6787
KasI G'GCGC_C
Cuts at: 3042 3042
Size: 6787
KpnI G_GTAC'C
Cuts at: 4112 4112
Size: 6787
MfeI C'AATT_G
Cuts at: 4386 4386
Size: 6787
MluI A'CGCG_T
Cuts at: 6663 6663
Size: 6787
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5901 6085 1305
Size: 4596 184 2007
Fragments arranged by size:
4596 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2009 2414 3854 10
Size: 54 1425 343 177 405 1440 2943
Fragments arranged by size:
2943 1440 1425 405 343 177 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2952 3188 3416 3968 4745 5104 5263
Size: 648 1786 236 228 552 777 359 159
Cuts at: 5263 518
Size: 2042
Fragments arranged by size:
2042 1786 777 648 552 359 236 228
159
MspA1I CmG'CkG
Cuts at: 1606 2514 3901 4203 4880 6028 6273 1606
Size: 908 1387 302 677 1148 245 2120
Fragments arranged by size:
2120 1387 1148 908 677 302 245
NarI GG'CG_CC
Cuts at: 3043 3043
Size: 6787
NcoI C'CATG_G
Cuts at: 513 1065 2004 2157 513
Size: 552 939 153 5143
Fragments arranged by size:
5143 939 552 153
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6787
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6787
NotI GC'GGCC_GC
Cuts at: 4234 4234
Size: 6787
NspI r_CATG'y
Cuts at: 1132 1229 2267 3277 4145 5690 1132
Size: 97 1038 1010 868 1545 2229
Fragments arranged by size:
2229 1545 1038 1010 868 97
PasI CC'CwG_GG
Cuts at: 1348 3518 1348
Size: 2170 4617
PciI A'CATG_T
Cuts at: 5686 5686
Size: 6787
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3038 3619 1108
Size: 1930 581 4276
Fragments arranged by size:
4276 1930 581
PfoI T'CCnGG_A
Cuts at: 2039 2619 3050 3453 2039
Size: 580 431 403 5373
Fragments arranged by size:
5373 580 431 403
PmeI GTTT'AAAC
Cuts at: 4091 4091
Size: 6787
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4856 4888 6369 6401 4856
Size: 32 1481 32 5242
Fragments arranged by size:
5242 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2043 3877 3906 2043
Size: 1834 29 4924
Fragments arranged by size:
4924 1834 29
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6787
PsiI TTA'TAA
Cuts at: 4357 4357
Size: 6787
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 3263 3295 3263
Size: 32 6755
PstI C_TGCA'G
Cuts at: 830 2145 2592 3927 4139 830
Size: 1315 447 1335 212 3478
Fragments arranged by size:
3478 1335 1315 447 212
PvuI CG_AT'CG
Cuts at: 2001 2481 2001
Size: 480 6307
PvuII CAG'CTG
Cuts at: 3901 3901
Size: 6787
SacI G_AGCT'C
Cuts at: 2671 2671
Size: 6787
SalI G'TCGA_C
Cuts at: 1936 4129 1936
Size: 2193 4594
SbfI CC_TGCA'GG
Cuts at: 4139 4139
Size: 6787
ScaI AGT'ACT
Cuts at: 5035 6747 5035
Size: 1712 5075
SexAI A'CCwGG_T
Cuts at: 3929 3929
Size: 6787
SfcI C'TryA_G
Cuts at: 826 1045 2141 2588 3923 4135 5270 5951 6142
Size: 219 1096 447 1335 212 1135 681 191
Cuts at: 6142 826
Size: 1471
Fragments arranged by size:
1471 1335 1135 1096 681 447 219 212
191
SfoI GGC'GCC
Cuts at: 3044 3044
Size: 6787
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6787
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6787
SmaI CCC'GGG
Cuts at: 1872 4054 4114 1872
Size: 2182 60 4545
Fragments arranged by size:
4545 2182 60
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2051 2324 2684 3374 3881 4891
Size: 197 926 108 273 360 690 507 1010
Cuts at: 4891 5792 6054 820
Size: 901 262 1553
Fragments arranged by size:
1553 1010 926 901 690 507 360 273
262 197 108
SnaBI TAC'GTA
Cuts at: 493 4578 493
Size: 4085 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6787
SphI G_CATG'C
Cuts at: 1132 4145 1132
Size: 3013 3774
SspI AAT'ATT
Cuts at: 5 52 4711 5
Size: 47 4659 2081
Fragments arranged by size:
4659 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6787
StyI C'CwwG_G
Cuts at: 513 1065 2004 2157 3294 3873 4226 513
Size: 552 939 153 1137 579 353 3074
Fragments arranged by size:
3074 1137 939 579 552 353 153
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2036 2085 3155 3626 3980 4814 4831
Size: 1255 456 49 1070 471 354 834 17
Cuts at: 4831 4984 5169 5614 325
Size: 153 185 445 1498
Fragments arranged by size:
1498 1255 1070 834 471 456 445 354
185 153 49 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2505 2928 3057
Size: 274 80 33 51 1443 528 423 129
Cuts at: 3057 3270 3561 3954 5033 6745 96
Size: 213 291 393 1079 1712 138
Fragments arranged by size:
1712 1443 1079 528 423 393 291 274
213 138 129 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6747 6779 6747
Size: 32 6755
Tth111I GACn'n_nGTC
Cuts at: 1316 2131 1316
Size: 815 5972
XbaI T'CTAG_A
Cuts at: 4123 4123
Size: 6787
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3793 4013 3793
Size: 220 6567
XhoI C'TCGA_G
Cuts at: 1943 2684 1943
Size: 741 6046
XmaI C'CCGG_G
Cuts at: 1870 4052 4112 1870
Size: 2182 60 4545
Fragments arranged by size:
4545 2182 60
XmnI GAAnn'nnTTC
Cuts at: 4916 4916
Size: 6787
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4595 276
Size: 53 83 186 936 3061 2468
Fragments arranged by size:
3061 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NarI
NcoI NdeI NheI NotI NspI PasI PciI PflMI
PfoI PmeI PpiI PpuMI PshAI PsiI PsrI PstI
PvuI PvuII SacI SalI SbfI ScaI SexAI SfcI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BfrBI BplI
BsiWI BstZ17I Bsu36I DraIII EcoRV FalI FseI FspAI
NaeI NgoMIV NruI NsiI PacI PmlI PspOMI PspXI
RsrII SacII SanDI SapI SfiI SrfI SwaI