Restriction map for
pFN21AE5752
(Linear) (Six-base) MAPSORT of: KIEE5752.seq Check: 8743 from: 1 to: 2082
KIEE5752.orf
With 182 enzymes: SgfI *
December 5, 2014 13:09 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 233 2082
Size: 233 1849
AccI GT'mk_AC
Cuts at: 0 1007 1148 2082
Size: 1007 141 934
Fragments arranged by size:
1007 934 141
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 856 1411 1450 1507 2082
Size: 856 555 39 57 575
Fragments arranged by size:
856 575 555 57 39
AflII C'TTAA_G
Cuts at: 0 319 2082
Size: 319 1763
AleI CACnn'nnGTG
Cuts at: 0 1183 2082
Size: 1183 899
AlwNI CAG_nnn'CTG
Cuts at: 0 569 1815 2082
Size: 569 1246 267
Fragments arranged by size:
1246 569 267
ApaLI G'TGCA_C
Cuts at: 0 262 1414 2082
Size: 262 1152 668
Fragments arranged by size:
1152 668 262
ApoI r'AATT_y
Cuts at: 0 856 2082
Size: 856 1226
AvaI C'yCGr_G
Cuts at: 0 679 2047 2082
Size: 679 1368 35
Fragments arranged by size:
1368 679 35
BanI G'GyrC_C
Cuts at: 0 1037 2082
Size: 1037 1045
BanII G_rGCy'C
Cuts at: 0 105 190 666 1201 2082
Size: 105 85 476 535 881
Fragments arranged by size:
881 535 476 105 85
BbeI G_GCGC'C
Cuts at: 0 1041 2082
Size: 1041 1041
BbsI GAAGACnn'nnnn_
Cuts at: 0 460 528 1351 2082
Size: 460 68 823 731
Fragments arranged by size:
823 731 460 68
BbvCI CC'TCA_GC
Cuts at: 0 94 1621 2082
Size: 94 1527 461
Fragments arranged by size:
1527 461 94
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1620 1654 2082
Size: 1620 34 428
Fragments arranged by size:
1620 428 34
BglI GCCn_nnn'nGGC
Cuts at: 0 1228 1588 1786 2082
Size: 1228 360 198 296
Fragments arranged by size:
1228 360 296 198
BglII A'GATC_T
Cuts at: 0 69 2082
Size: 69 2013
Bme1580I G_kGCm'C
Cuts at: 0 266 802 1418 2082
Size: 266 536 616 664
Fragments arranged by size:
664 616 536 266
BmrI ACTGGGnnnn_n'
Cuts at: 0 1602 2082
Size: 1602 480
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 18 1029 1813 2082
Size: 18 1011 784 269
Fragments arranged by size:
1011 784 269 18
Bpu10I CC'TnA_GC
Cuts at: 0 94 1621 2082
Size: 94 1527 461
Fragments arranged by size:
1527 461 94
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 31 1354 1897 2082
Size: 31 1323 543 185
Fragments arranged by size:
1323 543 185 31
BsaI GGTCTCn'nnnn_
Cuts at: 0 151 1096 2082
Size: 151 945 986
Fragments arranged by size:
986 945 151
BsaBI GATnn'nnATC
Cuts at: 0 74 1405 1558 2082
Size: 74 1331 153 524
Fragments arranged by size:
1331 524 153 74
BsaHI Gr'CG_yC
Cuts at: 0 1038 2082
Size: 1038 1044
BsaWI w'CCGG_w
Cuts at: 0 282 1313 2082
Size: 282 1031 769
Fragments arranged by size:
1031 769 282
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 112 142 2082
Size: 112 30 1940
Fragments arranged by size:
1940 112 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 133 457 460 1141 1810 1855 1894 2082
Size: 133 324 3 681 669 45 39 188
Fragments arranged by size:
681 669 324 188 133 45 39 3
BseYI C'CCAG_C
Cuts at: 0 1896 2082
Size: 1896 186
BsiEI CG_ry'CG
Cuts at: 0 476 2082
Size: 476 1606
BsiHKAI G_wGCw'C
Cuts at: 0 266 666 1370 1418 2082
Size: 266 400 704 48 664
Fragments arranged by size:
704 664 400 266 48
BsmI GAATG_Cn'
Cuts at: 0 954 1224 1652 2082
Size: 954 270 428 430
Fragments arranged by size:
954 430 428 270
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1294 1987 2082
Size: 1294 693 95
Fragments arranged by size:
1294 693 95
Bsp1286I G_dGCh'C
Cuts at: 0 105 190 266 666 802 1201 1370 1418
Size: 105 85 76 400 136 399 169 48
Cuts at: 1418 2082
Size: 664
Fragments arranged by size:
664 400 399 169 136 105 85 76
48
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 233 2082
Size: 233 1849
BsrDI GCAATG_nn'
Cuts at: 0 351 1197 2082
Size: 351 846 885
Fragments arranged by size:
885 846 351
BsrFI r'CCGG_y
Cuts at: 0 196 2082
Size: 196 1886
BsrGI T'GTAC_A
Cuts at: 0 923 1556 2082
Size: 923 633 526
Fragments arranged by size:
923 633 526
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1269 2082
Size: 1269 813
BstEII G'GTnAC_C
Cuts at: 0 1284 1602 2082
Size: 1284 318 480
Fragments arranged by size:
1284 480 318
BstXI CCAn_nnnn'nTGG
Cuts at: 0 168 780 1185 1296 2082
Size: 168 612 405 111 786
Fragments arranged by size:
786 612 405 168 111
BstYI r'GATC_y
Cuts at: 0 69 1811 2082
Size: 69 1742 271
Fragments arranged by size:
1742 271 69
BtgI C'CryG_G
Cuts at: 0 152 306 1583 2082
Size: 152 154 1277 499
Fragments arranged by size:
1277 499 154 152
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 507 1640 2082
Size: 507 1133 442
Fragments arranged by size:
1133 507 442
ClaI AT'CG_AT
Cuts at: 0 1835 2082
Size: 1835 247
DrdI GACnn_nn'nnGTC
Cuts at: 0 1003 2082
Size: 1003 1079
EaeI y'GGCC_r
Cuts at: 0 2 198 407 1580 1694 1847 2082
Size: 2 196 209 1173 114 153 235
Fragments arranged by size:
1173 235 209 196 153 114 2
EcoICRI GAG'CTC
Cuts at: 0 664 2082
Size: 664 1418
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 18 856 1029 1411 1450 1507 1813 2082
Size: 18 838 173 382 39 57 306 269
Fragments arranged by size:
838 382 306 269 173 57 39 18
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1738 2082
Size: 1738 344
EcoO109I rG'GnC_Cy
Cuts at: 0 38 566 1758 1788 1872 1901 2082
Size: 38 528 1192 30 84 29 181
Fragments arranged by size:
1192 528 181 84 38 30 29
EcoRI G'AATT_C
Cuts at: 0 856 2082
Size: 856 1226
FspI TGC'GCA
Cuts at: 0 241 2082
Size: 241 1841
HaeII r_GCGC'y
Cuts at: 0 398 1041 2082
Size: 398 643 1041
Fragments arranged by size:
1041 643 398
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 109 112 141 144 571 603 745 777
Size: 109 3 29 3 427 32 142 32
Cuts at: 777 1796 1828 2082
Size: 1019 32 254
Fragments arranged by size:
1019 427 254 142 109 32 32 32
29 3 3
KasI G'GCGC_C
Cuts at: 0 1037 2082
Size: 1037 1045
MscI TGG'CCA
Cuts at: 0 4 409 1849 2082
Size: 4 405 1440 233
Fragments arranged by size:
1440 405 233 4
MslI CAynn'nnrTG
Cuts at: 0 947 1183 1411 1963 2082
Size: 947 236 228 552 119
Fragments arranged by size:
947 552 236 228 119
MspA1I CmG'CkG
Cuts at: 0 509 1896 2082
Size: 509 1387 186
Fragments arranged by size:
1387 509 186
NarI GG'CG_CC
Cuts at: 0 1038 2082
Size: 1038 1044
NcoI C'CATG_G
Cuts at: 0 152 2082
Size: 152 1930
NspI r_CATG'y
Cuts at: 0 262 1272 2082
Size: 262 1010 810
Fragments arranged by size:
1010 810 262
PasI CC'CwG_GG
Cuts at: 0 1513 2082
Size: 1513 569
PflMI CCAn_nnn'nTGG
Cuts at: 0 1033 1614 2082
Size: 1033 581 468
Fragments arranged by size:
1033 581 468
PfoI T'CCnGG_A
Cuts at: 0 34 614 1045 1448 2082
Size: 34 580 431 403 634
Fragments arranged by size:
634 580 431 403 34
PpuMI rG'GwC_Cy
Cuts at: 0 38 1872 1901 2082
Size: 38 1834 29 181
Fragments arranged by size:
1834 181 38 29
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 1258 1290 2082
Size: 1258 32 792
Fragments arranged by size:
1258 792 32
PstI C_TGCA'G
Cuts at: 0 140 587 1922 2082
Size: 140 447 1335 160
Fragments arranged by size:
1335 447 160 140
PvuI CG_AT'CG
Cuts at: 0 476 2082
Size: 476 1606
PvuII CAG'CTG
Cuts at: 0 1896 2082
Size: 1896 186
SacI G_AGCT'C
Cuts at: 0 666 2082
Size: 666 1416
SexAI A'CCwGG_T
Cuts at: 0 1924 2082
Size: 1924 158
SfcI C'TryA_G
Cuts at: 0 136 583 1918 2082
Size: 136 447 1335 164
Fragments arranged by size:
1335 447 164 136
SfoI GGC'GCC
Cuts at: 0 1039 2082
Size: 1039 1043
SmaI CCC'GGG
Cuts at: 0 2049 2082
Size: 2049 33
SmlI C'TyrA_G
Cuts at: 0 46 319 679 1369 1876 2082
Size: 46 273 360 690 507 206
Fragments arranged by size:
690 507 360 273 206 46
StyI C'CwwG_G
Cuts at: 0 152 1289 1868 2082
Size: 152 1137 579 214
Fragments arranged by size:
1137 579 214 152
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 31 80 1150 1621 1975 2082
Size: 31 49 1070 471 354 107
Fragments arranged by size:
1070 471 354 107 49 31
TatI w'GTAC_w
Cuts at: 0 500 923 1052 1265 1556 1949 2082
Size: 500 423 129 213 291 393 133
Fragments arranged by size:
500 423 393 291 213 133 129
Tth111I GACn'n_nGTC
Cuts at: 0 126 2082
Size: 126 1956
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1788 2008 2082
Size: 1788 220 74
Fragments arranged by size:
1788 220 74
XhoI C'TCGA_G
Cuts at: 0 679 2082
Size: 679 1403
XmaI C'CCGG_G
Cuts at: 0 2047 2082
Size: 2047 35
Enzymes that do cut:
AarI AccI AcuI AflII AleI AlwNI ApaLI ApoI
AvaI BanI BanII BbeI BbsI BbvCI BcgI BglI
BglII Bme1580I BmrI BpmI Bpu10I BpuEI BsaI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspMI BsrDI BsrFI BsrGI BstAPI BstEII
BstXI BstYI BtgI BtgZI ClaI DrdI EaeI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I KasI
MscI MslI MspA1I NarI NcoI NspI PasI PflMI
PfoI PpuMI PsrI PstI PvuI PvuII SacI SexAI
SfcI SfoI SmaI SmlI StyI TaqII TatI Tth111I
XcmI XhoI XmaI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AflIII AgeI AhdI AlfI
AloI ApaI AscI AseI AsiSI AvrII BaeI BamHI
BciVI BclI BfrBI BlpI BmgBI BmtI BplI BsaAI
BsgI BsiWI BspEI BspHI BsrBI BssHII BssSI BstBI
BstZ17I Bsu36I BtsI CspCI DraI DraIII EagI EarI
EciI EcoRV FalI FseI FspAI HincII HindIII HpaI
KpnI MfeI MluI MmeI NaeI NdeI NgoMIV NheI
NotI NruI NsiI PacI PciI PmeI PmlI PpiI
PshAI PsiI PspOMI PspXI RsrII SacII SalI SanDI
SapI SbfI ScaI SfiI SgfI SgrAI SnaBI SpeI
SphI SrfI SspI StuI SwaI TstI XbaI XmnI
ZraI