Restriction map for
pFN21AE3958
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# NO CUT ENZYMES ( 140 enzymes) #
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AatII Acc65I AccII AccIII AclI
AcyI AflII AflIII AgeI AhdI
Alw44I AocI Aor51HI ApaI ApaLI
AscI AseI AsnI Asp718I AspEI
AvaI AviII AvrII BamHI BanI
BbeI BbrPI BcgI BciVI BfaI
BfrI BglI BlnI BlpI Bpu1102I
BsaAI BsaBI BsaHI BsaI BseAI
BsiEI BsiWI BsmBI BsmI BsoBI
BspDI BspEI BspHI BspLU11I BsrBI
BsrFI BssHII BssSI Bst1107I BstAPI
BstBI BstEII BstPI BstUI BstXI
BstZ17I Bsu36I CelII CfoI Cfr10I
ClaI CpoI DraI DraIII DrdI
DsaI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoO65I EcoRV EcoT14I
EcoT22I FauI FseI FspI HaeII
HhaI Hin1I HinP1I HindIII KasI
KpnI KspI MaeI MamI MfeI
MluI MroI MslI MunI MvnI
NaeI NarI NcoI NgoMIV NheI
NotI NruI NsiI NspV PacI
PaeR7I PinAI PmaCI PmeI PmlI
PshBI Psp1406I PvuI RcaI RsrII
SacII SalI SapI SbfI ScaI
SfiI SfoI SfuI SgrAI SmaI
SmlI SnaBI SpeI Sse8387I StyI
SwaI XbaI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 51 enzymes) #
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AccI 67 GTAGAC GT^[AC][GT]AC
AciI 849 CCGC CCGC(-3/-1)
AfaI 837 GTAC GT^AC
AlwI 657 GGATC GGATC(4/5)
Asp700I 300 GAAGATGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 70 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BalI 1329 TGGCCA TGG^CCA
BbsI 991 GTCTTC GAAGAC(2/6)
BbvCI 397 GCTGAGG CCTCAGC(-5/-2)
BclI 533 TGATCA T^GATCA
BglII 958 AGATCT A^GATCT
BpuAI 991 GTCTTC GAAGAC(2/6)
BsaJI 986 CCAGGG C^C[ACGT][ACGT]GG
BsaWI 561 TCCGGT [AT]^CCGG[AT]
BsgI 778 CTGCAC GTGCAG(16/14)
BsmFI 280 GGGAC GGGAC(10/14)
Bsp1407I 836 TGTACA T^GTACA
BspMI 40 ACCTGC ACCTGC(4/8)
BsrGI 836 TGTACA T^GTACA
BstYI 958 AGATCT [GA]^GATC[CT]
EaeI 1329 TGGCCA [CT]^GGCC[GA]
EarI 388 GAAGAG CTCTTC(1/4)
EcoNI 1070 CCTGGATAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 948 GAATTC G^AATTC
FbaI 533 TGATCA T^GATCA
HgaI 44 GCGTC GACGC(5/10)
HincII 1096 GTTAAC GT[CT]^[GA]AC
HindII 1096 GTTAAC GT[CT]^[GA]AC
HpaI 1096 GTTAAC GTT^AAC
Ksp632I 388 GAAGAG CTCTTC(1/4)
MaeII 936 ACGT A^CGT
MflI 958 AGATCT [GA]^GATC[CT]
MluNI 1329 TGGCCA TGG^CCA
MscI 1329 TGGCCA TGG^CCA
NdeI 1272 CATATG CA^TATG
PflFI 70 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshAI 319 GACAAGAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI 812 CTGCAG CTGCA^G
RsaI 837 GTAC GT^AC
SexAI 62 ACCTGGT A^CC[AT]GGT
SfaNI 558 GCATC GCATC(5/9)
SphI 160 GCATGC GCATG^C
SspBI 836 TGTACA T^GTACA
SspI 199 AATATT AAT^ATT
StuI 1312 AGGCCT AGG^CCT
TaqI 386 TCGA T^CGA
Tth111I 70 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 386 TCGA T^CGA
XcmI 821 CCATCACTGGACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 958 AGATCT [GA]^GATC[CT]
XmnI 300 GAAGATGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 13 enzymes) #
###################################
AlwNI 96 CAGCTTCTG CAG[ACGT][ACGT][ACGT]^CTG
240 CAGTCTCTG
BcnI 408 CCGGG CC^[GC]GG
1203 CCGGG
BseRI 328 CTCCTC GAGGAG(10/8)
478 GAGGAG
BsrDI 225 CATTGC GCAATG(2/0)
509 GCAATG
MspA1I 395 CAGCTG C[AC]G^C[GT]G
1286 CAGCTG
NciI 408 CCGGG CC^[GC]GG
1203 CCGGG
PflMI 239 CCAGTCTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
735 CCAGGATTTGG
PpuMI 280 GGGACCC [GA]G^G[AT]CC[CT]
816 AGGACCC
PvuII 395 CAGCTG CAG^CTG
1286 CAGCTG
SacI 258 GAGCTC GAGCT^C
805 GAGCTC
SfcI 102 CTGTAG C^T[GA][CT]AG
812 CTGCAG
TfiI 450 GATTC G^A[AT]TC
798 GAATC
Van91I 239 CCAGTCTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
735 CCAGGATTTGG
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# 3 OR MORE CUT ENZYMES ( 54 enzymes) #
#########################################
AcsI AluI ApoI AspHI AvaII
BanII BbvI BmyI BpmI BsiHKAI
BsiYI BslI BsmAI Bsp1286I BsrI
BssKI BstF5I BstNI Cac8I Cfr13I
DdeI DpnII DraII EcoO109I EcoRII
Fnu4HI FokI HaeIII HapII HinfI
HpaII HphI ItaI MaeIII MboI
MboII MnlI MseI MspI MvaI
MwoI NdeII NlaIII NlaIV NspI
PleI Sau3AI Sau96I ScrFI Tru9I
TseI Tsp45I Tsp509I TspRI