(Circular) (Six-base) MAPSORT of: pFN21AE3958.seq Check: 7150 from: 1 to: 6043 pFN21AE3958 6043 bp With 182 enzymes: SgfI * March 28, 2012 11:52 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2054 2054 Size: 6043 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3853 278 Size: 53 83 186 936 2317 2468 Fragments arranged by size: 2468 2317 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2073 3386 1937 Size: 136 1313 4594 Fragments arranged by size: 4594 1313 136 Acc65I G'GTAC_C Cuts at: 3364 3364 Size: 6043 AclI AA'CG_TT Cuts at: 1563 4170 4543 1563 Size: 2607 373 3063 Fragments arranged by size: 3063 2607 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2234 3155 4106 5490 5758 1632 Size: 602 921 951 1384 268 1917 Fragments arranged by size: 1917 1384 951 921 602 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5846 AflIII A'CryG_T Cuts at: 1203 4942 5919 1203 Size: 3739 977 1327 Fragments arranged by size: 3739 1327 977 AgeI A'CCGG_T Cuts at: 4874 4874 Size: 6043 AhdI GACnn_n'nnGTC Cuts at: 4772 4772 Size: 6043 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6043 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6043 AlwNI CAG_nnn'CTG Cuts at: 2106 2250 3786 5358 2106 Size: 144 1536 1572 2791 Fragments arranged by size: 2791 1572 1536 144 ApaLI G'TGCA_C Cuts at: 4100 5256 4100 Size: 1156 4887 ApoI r'AATT_y Cuts at: 1493 2218 2868 2953 3352 3578 3730 5839 1493 Size: 725 650 85 399 226 152 2109 1697 Fragments arranged by size: 2109 1697 725 650 399 226 152 85 AseI AT'TA_AT Cuts at: 160 1032 4597 160 Size: 872 3565 1606 Fragments arranged by size: 3565 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6043 AvaI C'yCGr_G Cuts at: 1870 1943 3368 1870 Size: 73 1425 4545 Fragments arranged by size: 4545 1425 73 BamHI G'GATC_C Cuts at: 3373 3740 3373 Size: 367 5676 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3364 4819 618 Size: 325 217 627 1577 1455 1842 Fragments arranged by size: 1842 1577 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2267 2374 2814 3055 3362 1391 Size: 876 107 440 241 307 4072 Fragments arranged by size: 4072 876 440 307 241 107 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2989 928 Size: 971 1090 3982 Fragments arranged by size: 3982 1090 971 BbvCI CC'TCA_GC Cuts at: 2403 2403 Size: 6043 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4234 4268 1903 Size: 34 2297 34 3678 Fragments arranged by size: 3678 2297 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3935 5145 3935 Size: 1210 4833 BclI T'GATC_A Cuts at: 1548 2538 1548 Size: 990 5053 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4654 136 Size: 107 122 71 631 605 2982 1525 Fragments arranged by size: 2982 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2963 6038 2963 Size: 3075 2968 BlpI GC'TnA_GC Cuts at: 3460 3460 Size: 6043 Bme1580I G_kGCm'C Cuts at: 1790 2100 2533 4104 5260 1790 Size: 310 433 1571 1156 2573 Fragments arranged by size: 2573 1571 1156 433 310 BmgBI CAC'GTC Cuts at: 1318 2942 1318 Size: 1624 4419 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3028 3058 3167 Size: 416 53 83 399 24 1611 30 109 Cuts at: 3167 3774 4732 442 Size: 607 958 1753 Fragments arranged by size: 1753 1611 958 607 416 399 109 83 53 30 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6043 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2527 2624 2723 4703 1239 Size: 1288 97 99 1980 2579 Fragments arranged by size: 2579 1980 1288 99 97 Bpu10I CC'TnA_GC Cuts at: 2261 2403 2261 Size: 142 5901 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4168 5033 5331 4168 Size: 865 298 4880 Fragments arranged by size: 4880 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4706 882 Size: 617 3207 2219 Fragments arranged by size: 3207 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3834 493 Size: 711 2630 2702 Fragments arranged by size: 2702 2630 711 BsaBI GATnn'nnATC Cuts at: 6037 6037 Size: 6043 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3850 4232 4867 275 Size: 53 83 186 936 2317 382 635 1451 Fragments arranged by size: 2317 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2566 4476 4874 5148 5295 5706 2566 Size: 1910 398 274 147 411 2903 Fragments arranged by size: 2903 1910 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2742 2772 2979 3009 1178 Size: 30 165 30 1339 30 207 30 4212 Fragments arranged by size: 4212 1339 207 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2324 2498 1186 Size: 1138 174 4731 Fragments arranged by size: 4731 1138 174 BseYI C'CCAG_C Cuts at: 2793 3289 5246 5742 2793 Size: 496 1957 496 3094 Fragments arranged by size: 3094 1957 496 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2768 1163 Size: 573 1032 4438 Fragments arranged by size: 4438 1032 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3493 4254 5282 1540 Size: 275 186 1492 761 1028 2301 Fragments arranged by size: 2301 1492 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2267 2351 2367 2814 4104 4189 5260 2267 Size: 84 16 447 1290 85 1071 3050 Fragments arranged by size: 3050 1290 1071 447 85 84 16 BsmI GAATG_Cn' Cuts at: 3554 3647 3554 Size: 93 5950 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2100 2267 2351 2367 2374 2533 2814 Size: 399 310 167 84 16 7 159 281 Cuts at: 2814 3055 3362 4104 4189 5260 1391 Size: 241 307 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 310 307 281 241 167 159 85 84 16 7 BspEI T'CCGG_A Cuts at: 5706 5706 Size: 6043 BspHI T'CATG_A Cuts at: 3932 5662 3932 Size: 1730 4313 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2054 3398 844 Size: 1210 1344 3489 Fragments arranged by size: 3489 1344 1210 BsrBI CCG'CTC Cuts at: 1394 1823 3930 1394 Size: 429 2107 3507 Fragments arranged by size: 3507 2107 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2229 2521 4538 4712 65 Size: 1183 981 292 2017 174 1396 Fragments arranged by size: 2017 1396 1183 981 292 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4687 4874 1700 Size: 110 2877 187 2869 Fragments arranged by size: 2877 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 2841 96 Size: 2745 3298 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6043 BssSI C'ACGA_G Cuts at: 4103 5115 4103 Size: 1012 5031 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6043 BstBI TT'CG_AA Cuts at: 4847 4847 Size: 6043 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6043 BstYI r'GATC_y Cuts at: 1972 2963 3373 3740 4125 4142 5594 5680 5692 Size: 991 410 367 385 17 1452 86 12 Cuts at: 5692 6038 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 991 410 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 4552 Fragments arranged by size: 4552 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5304 Fragments arranged by size: 5304 683 56 BtsI GCAGTG_nn' Cuts at: 2814 3559 4353 4373 2814 Size: 745 794 20 4484 Fragments arranged by size: 4484 794 745 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6043 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6008 DraI TTT'AAA Cuts at: 3347 3347 Size: 6043 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5050 809 Size: 507 3734 1802 Fragments arranged by size: 3734 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3334 3490 4379 Size: 54 1425 50 275 18 1504 156 889 Cuts at: 4379 6008 8 Size: 1629 43 Fragments arranged by size: 1629 1504 1425 889 275 156 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3490 1537 Size: 275 1678 4090 Fragments arranged by size: 4090 1678 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2388 3973 1353 Size: 1035 1585 3423 Fragments arranged by size: 3423 1585 1035 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4630 5004 5150 387 Size: 1064 138 3041 374 146 1280 Fragments arranged by size: 3041 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2265 2812 2265 Size: 547 5496 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2234 2527 2624 2723 3155 4106 4703 Size: 393 602 293 97 99 432 951 597 Cuts at: 4703 5490 5758 1239 Size: 787 268 1524 Fragments arranged by size: 1524 951 787 602 597 432 393 293 268 99 97 EcoNI CCTnn'n_nnAGG Cuts at: 3079 3079 Size: 6043 EcoO109I rG'GnC_Cy Cuts at: 2112 2286 2822 2112 Size: 174 536 5333 Fragments arranged by size: 5333 536 174 EcoRI G'AATT_C Cuts at: 2953 3352 3730 2953 Size: 399 378 5266 Fragments arranged by size: 5266 399 378 FspI TGC'GCA Cuts at: 4549 4549 Size: 6043 HaeII r_GCGC'y Cuts at: 1678 5190 1678 Size: 3512 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2979 3011 4684 4716 4758 Size: 32 135 32 1407 32 1673 32 42 Cuts at: 4758 4790 1373 Size: 32 2626 Fragments arranged by size: 2626 1673 1407 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3103 3387 3633 669 Size: 988 281 1165 284 246 3079 Fragments arranged by size: 3079 1165 988 284 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6043 HpaI GTT'AAC Cuts at: 3103 3633 3103 Size: 530 5513 KpnI G_GTAC'C Cuts at: 3368 3368 Size: 6043 MfeI C'AATT_G Cuts at: 3642 3642 Size: 6043 MluI A'CGCG_T Cuts at: 5919 5919 Size: 6043 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2376 2909 5157 5341 1305 Size: 1071 533 2248 184 2007 Fragments arranged by size: 2248 2007 1071 533 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3336 10 Size: 54 1425 343 1504 2717 Fragments arranged by size: 2717 1504 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 4001 4360 4519 518 Size: 648 2835 359 159 2042 Fragments arranged by size: 2835 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2402 3293 3459 4136 5284 5529 1606 Size: 796 891 166 677 1148 245 2120 Fragments arranged by size: 2120 1148 891 796 677 245 166 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4552 Fragments arranged by size: 4552 939 552 NdeI CA'TA_TG Cuts at: 387 3278 387 Size: 2891 3152 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6043 NotI GC'GGCC_GC Cuts at: 3490 3490 Size: 6043 NspI r_CATG'y Cuts at: 1132 1229 2027 2169 2791 3401 4946 1132 Size: 97 798 142 622 610 1545 2229 Fragments arranged by size: 2229 1545 798 622 610 142 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6043 PciI A'CATG_T Cuts at: 4942 4942 Size: 6043 PflMI CCAn_nnn'nTGG Cuts at: 1108 2250 2746 1108 Size: 1142 496 4405 Fragments arranged by size: 4405 1142 496 PfoI T'CCnGG_A Cuts at: 2739 3074 2739 Size: 335 5708 PmeI GTTT'AAAC Cuts at: 3347 3347 Size: 6043 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4112 4144 5625 5657 4112 Size: 32 1481 32 4498 Fragments arranged by size: 4498 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2286 2822 2286 Size: 536 5507 PshAI GACnn'nnGTC Cuts at: 1613 2328 1613 Size: 715 5328 PsiI TTA'TAA Cuts at: 3613 3613 Size: 6043 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2600 2632 2600 Size: 32 6011 PstI C_TGCA'G Cuts at: 830 2821 3395 830 Size: 1991 574 3478 Fragments arranged by size: 3478 1991 574 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6043 PvuII CAG'CTG Cuts at: 2402 3293 2402 Size: 891 5152 SacI G_AGCT'C Cuts at: 2267 2814 2267 Size: 547 5496 SalI G'TCGA_C Cuts at: 1936 3385 1936 Size: 1449 4594 SanDI GG'GwC_CC Cuts at: 2286 2286 Size: 6043 SbfI CC_TGCA'GG Cuts at: 3395 3395 Size: 6043 ScaI AGT'ACT Cuts at: 4291 6003 4291 Size: 1712 4331 SexAI A'CCwGG_T Cuts at: 2067 2067 Size: 6043 SfcI C'TryA_G Cuts at: 826 1045 2107 2817 3391 4526 5207 5398 826 Size: 219 1062 710 574 1135 681 191 1471 Fragments arranged by size: 1471 1135 1062 710 681 574 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6043 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6043 SmaI CCC'GGG Cuts at: 1872 3370 1872 Size: 1498 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4147 5048 5310 820 Size: 197 926 2204 901 262 1553 Fragments arranged by size: 2204 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3834 493 Size: 3341 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6043 SphI G_CATG'C Cuts at: 1132 2169 3401 1132 Size: 1037 1232 3774 Fragments arranged by size: 3774 1232 1037 SspI AAT'ATT Cuts at: 5 52 2206 3967 5 Size: 47 2154 1761 2081 Fragments arranged by size: 2154 2081 1761 47 StuI AGG'CCT Cuts at: 1517 3319 1517 Size: 1802 4241 StyI C'CwwG_G Cuts at: 513 1065 2004 3482 513 Size: 552 939 1478 3074 Fragments arranged by size: 3074 1478 939 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4070 4087 4240 4425 4870 325 Size: 1255 2490 17 153 185 445 1498 Fragments arranged by size: 2490 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2841 4289 6001 Size: 274 80 33 51 1443 864 1448 1712 Cuts at: 6001 96 Size: 138 Fragments arranged by size: 1712 1448 1443 864 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2013 2045 2742 2774 6003 6035 2013 Size: 32 697 32 3229 32 2021 Fragments arranged by size: 3229 2021 697 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2078 1316 Size: 762 5281 XbaI T'CTAG_A Cuts at: 3379 3379 Size: 6043 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2833 2833 Size: 6043 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6043 XmaI C'CCGG_G Cuts at: 1870 3368 1870 Size: 1498 4545 XmnI GAAnn'nnTTC Cuts at: 2309 4172 2309 Size: 1863 4180 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3851 276 Size: 53 83 186 936 2317 2468 Fragments arranged by size: 2468 2317 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PsrI PstI PvuI PvuII SacI SalI SanDI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspOMI PspXI RsrII SacII SapI SfiI SfoI SrfI SwaI