################################## # NO CUT ENZYMES ( 161 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcsI AflII AflIII AgeI AhdI AocI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspI AviII AvrII BalI BamHI BbrPI BbsI BbvCI BcgI BciVI BclI BfrI BglII BlnI BlpI Bpu1102I BpuAI BsaAI BsaBI BsaI BsaWI BseAI BseRI BsgI BsiEI BsiWI BsmI BspEI BspHI BspLU11I BspMI BsrBI BsrDI BsrFI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstYI BstZ17I Bsu36I CelII Cfr10I CpoI DraI DraIII DrdI DsaI EagI Eam1105I EarI EclXI Eco52I Eco81I EcoNI EcoO65I EcoRI EcoRV EcoT22I FbaI FseI FspI HgaI HincII HindII HindIII HinfI HpaI KpnI Ksp632I KspI MamI MfeI MflI MluI MluNI MroI MscI MslI MunI MvnI NaeI NcoI NdeI NgoMIV NotI NruI NsiI NspV PacI PaeR7I PflFI PflMI PinAI PleI PmaCI PmeI PmlI PshAI PshBI Psp1406I PstI PvuI PvuII RcaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfcI SfiI SfuI SgrAI SmaI SmlI SnaBI SpeI Sse8387I SspI StuI SwaI TfiI Tsp45I Tsp509I TspRI Tth111I Van91I XbaI XcmI XhoI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 50 enzymes) # ################################### AcyI 487 GGCGCC G[GA]^CG[CT]C Alw44I 355 GTGCAC G^TGCAC AlwI 11 GGATC GGATC(4/5) AlwNI 392 CAGCGGCTG CAG[ACGT][ACGT][ACGT]^CTG Aor51HI 580 AGCGCT AGC^GCT ApaI 504 GGGCCC GGGCC^C ApaLI 355 GTGCAC G^TGCAC AspHI 355 GTGCAC G[AT]GC[AT]^C AvaI 501 CTCGGG C^[CT]CG[GA]G BanII 504 GGGCCC G[GA]GC[CT]^C BbeI 487 GGCGCC GGCGC^C BcnI 517 CCGGG CC^[GC]GG BfaI 584 CTAG C^TAG BglI 388 GCCACAGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BsaHI 487 GGCGCC G[GA]^CG[CT]C BsiHKAI 355 GTGCAC G[AT]GC[AT]^C BsmBI 482 GAGACG CGTCTC(1/5) BsmFI 417 GGGAC GGGAC(10/14) BsoBI 501 CTCGGG C^[CT]CG[GA]G BspDI 307 ATCGAT AT^CGAT BsrI 122 ACTGG ACTGG(1/-1) BstXI 20 CCAGGCTGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG ClaI 307 ATCGAT AT^CGAT DpnII 12 GATC ^GATC Eco47III 580 AGCGCT AGC^GCT EcoT14I 413 CCAAGG C^C[AT][AT]GG HapII 517 CCGG C^CGG Hin1I 487 GGCGCC G[GA]^CG[CT]C HpaII 517 CCGG C^CGG KasI 487 GGCGCC G^GCGCC MaeI 584 CTAG C^TAG MaeII 380 ACGT A^CGT MaeIII 341 GTTAC ^GT[ACGT]AC MboI 12 GATC ^GATC MseI 95 TTAA T^TAA MspI 517 CCGG C^CGG NarI 487 GGCGCC GG^CGCC NciI 517 CCGGG CC^[GC]GG NdeII 12 GATC ^GATC NheI 583 GCTAGC G^CTAGC NspI 455 GCATGC [GA]CATG^[CT] PpuMI 461 AGGACCT [GA]G^G[AT]CC[CT] Sau3AI 12 GATC ^GATC SfaNI 346 GATGC GCATC(5/9) SfoI 487 GGCGCC GGC^GCC SphI 455 GCATGC GCATG^C StyI 413 CCAAGG C^C[AT][AT]GG TaqI 308 TCGA T^CGA Tru9I 95 TTAA T^TAA TthHB8I 308 TCGA T^CGA ################################### # TWO CUT ENZYMES ( 22 enzymes) # ################################### AluI 448 AGCT AG^CT 589 AGCT AvaII 321 GGTCC G^G[AT]CC 462 GGACC BmyI 355 GTGCAC G[AGT]GC[ACT]^C 504 GGGCCC BpmI 18 CTCCAG CTGGAG(16/14) 249 CTCCAG Bsp1286I 355 GTGCAC G[AGT]GC[ACT]^C 504 GGGCCC Bsp1407I 260 TGTACA T^GTACA 273 TGTACA BsrGI 260 TGTACA T^GTACA 273 TGTACA BstF5I 277 CATCC GGATG(2/0) 113 GGATG CfoI 488 GCGC GCG^C 581 GCGC DraII 461 AGGACCT [GA]G^G[ACGT]CC[CT] 504 GGGCCCC EaeI 383 TGGCCG [CT]^GGCC[GA] 546 CGGCCA EcoO109I 461 AGGACCT [GA]G^G[ACGT]CC[CT] 504 GGGCCCC FauI 369 GCGGG CCCGC(4/6) 528 GCGGG FokI 277 CATCC GGATG(9/13) 113 GGATG HaeII 487 GGCGCC [GA]GCGC^[CT] 580 AGCGCT HhaI 488 GCGC GCG^C 581 GCGC HinP1I 488 GCGC G^CGC 581 GCGC HphI 143 TCACC GGTGA(8/7) 467 TCACC MboII 170 TCTTC GAAGA(8/7) 289 TCTTC MspA1I 392 CAGCGG C[AC]G^C[GT]G 534 CAGCGG NlaIII 8 CATG CATG^ 456 CATG SspBI 260 TGTACA T^GTACA 273 TGTACA ######################################### # 3 OR MORE CUT ENZYMES ( 25 enzymes) # ######################################### AciI AfaI BanI BbvI BsaJI BsiYI BslI BsmAI BssKI BstNI Cac8I Cfr13I DdeI EcoRII Fnu4HI HaeIII ItaI MnlI MvaI MwoI NlaIV RsaI Sau96I ScrFI TseI