(Circular) (Six-base) MAPSORT of: pFN21AE6573.seq Check: 508 from: 1 to: 5308 pFN21AE6573 5308 bp With 182 enzymes: SgfI * March 18, 2015 14:53 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3118 278 Size: 53 83 186 936 1582 2468 Fragments arranged by size: 2468 1582 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2651 1937 Size: 714 4594 Acc65I G'GTAC_C Cuts at: 2629 2629 Size: 5308 AclI AA'CG_TT Cuts at: 1563 3435 3808 1563 Size: 1872 373 3063 Fragments arranged by size: 3063 1872 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2440 3371 4755 5023 1632 Size: 808 931 1384 268 1917 Fragments arranged by size: 1917 1384 931 808 268 AfeI AGC'GCT Cuts at: 2587 2587 Size: 5308 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5111 AflIII A'CryG_T Cuts at: 1203 4207 5184 1203 Size: 3004 977 1327 Fragments arranged by size: 3004 1327 977 AgeI A'CCGG_T Cuts at: 4139 4139 Size: 5308 AhdI GACnn_n'nnGTC Cuts at: 4037 4037 Size: 5308 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5308 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5308 AlwNI CAG_nnn'CTG Cuts at: 2402 3051 4623 2402 Size: 649 1572 3087 Fragments arranged by size: 3087 1572 649 ApaI G_GGCC'C Cuts at: 2513 2513 Size: 5308 ApaLI G'TGCA_C Cuts at: 2360 3365 4521 2360 Size: 1005 1156 3147 Fragments arranged by size: 3147 1156 1005 ApoI r'AATT_y Cuts at: 1493 2617 2843 2995 5104 1493 Size: 1124 226 152 2109 1697 Fragments arranged by size: 2109 1697 1124 226 152 AseI AT'TA_AT Cuts at: 160 1032 3862 160 Size: 872 2830 1606 Fragments arranged by size: 2830 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5308 AvaI C'yCGr_G Cuts at: 1870 1943 2506 2633 1870 Size: 73 563 127 4545 Fragments arranged by size: 4545 563 127 73 BamHI G'GATC_C Cuts at: 2638 3005 2638 Size: 367 4941 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2048 2429 2492 2600 2629 Size: 325 217 627 261 381 63 108 29 Cuts at: 2629 4084 618 Size: 1455 1842 Fragments arranged by size: 1842 1455 627 381 325 261 217 108 63 29 BanII G_rGCy'C Cuts at: 1391 2513 2627 1391 Size: 1122 114 4072 Fragments arranged by size: 4072 1122 114 BbeI G_GCGC'C Cuts at: 2496 2496 Size: 5308 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4337 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3499 3533 1903 Size: 34 1562 34 3678 Fragments arranged by size: 3678 1562 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3200 4410 3200 Size: 1210 4098 BclI T'GATC_A Cuts at: 1548 1548 Size: 5308 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2399 3919 136 Size: 107 122 71 631 605 727 1520 1525 Fragments arranged by size: 1525 1520 727 631 605 122 107 71 BglII A'GATC_T Cuts at: 5303 5303 Size: 5308 BlpI GC'TnA_GC Cuts at: 2725 2725 Size: 5308 Bme1580I G_kGCm'C Cuts at: 1790 2364 2513 3369 4525 1790 Size: 574 149 856 1156 2573 Fragments arranged by size: 2573 1156 856 574 149 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5308 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3039 3997 442 Size: 416 53 83 399 24 1622 958 1753 Fragments arranged by size: 1753 1622 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 2592 1056 Size: 1536 3772 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2008 2239 3968 1239 Size: 769 231 1729 2579 Fragments arranged by size: 2579 1729 769 231 Bpu10I CC'TnA_GC Cuts at: 2596 2596 Size: 5308 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3433 4298 4596 3433 Size: 865 298 4145 Fragments arranged by size: 4145 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3971 882 Size: 617 2472 2219 Fragments arranged by size: 2472 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3099 493 Size: 711 1895 2702 Fragments arranged by size: 2702 1895 711 BsaBI GATnn'nnATC Cuts at: 5302 5302 Size: 5308 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2493 3115 3497 4132 Size: 53 83 186 936 960 622 382 635 Cuts at: 4132 275 Size: 1451 Fragments arranged by size: 1451 960 936 635 622 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3741 4139 4413 4560 4971 3741 Size: 398 274 147 411 4078 Fragments arranged by size: 4078 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5083 Fragments arranged by size: 5083 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5308 BseYI C'CCAG_C Cuts at: 4511 5007 4511 Size: 496 4812 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 4735 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2758 3519 4547 1540 Size: 275 186 757 761 1028 2301 Fragments arranged by size: 2301 1028 761 757 275 186 BsiHKAI G_wGCw'C Cuts at: 2364 3369 3454 4525 2364 Size: 1005 85 1071 3147 Fragments arranged by size: 3147 1071 1005 85 BsmI GAATG_Cn' Cuts at: 2819 2912 2819 Size: 93 5215 BsmBI CGTCTCn'nnnn_ Cuts at: 2481 2481 Size: 5308 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2364 2513 2627 3369 3454 4525 1391 Size: 399 574 149 114 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 574 399 149 114 85 BspEI T'CCGG_A Cuts at: 4971 4971 Size: 5308 BspHI T'CATG_A Cuts at: 3197 4927 3197 Size: 1730 3578 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2663 844 Size: 1819 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3195 1394 Size: 429 1372 3507 Fragments arranged by size: 3507 1372 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3803 3977 65 Size: 1183 2555 174 1396 Fragments arranged by size: 2555 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3952 4139 1700 Size: 110 2142 187 2869 Fragments arranged by size: 2869 2142 187 110 BsrGI T'GTAC_A Cuts at: 96 2265 2278 96 Size: 2169 13 3126 Fragments arranged by size: 3126 2169 13 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5308 BssSI C'ACGA_G Cuts at: 3368 4380 3368 Size: 1012 4296 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5308 BstBI TT'CG_AA Cuts at: 4112 4112 Size: 5308 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5308 BstXI CCAn_nnnn'nTGG Cuts at: 2032 2032 Size: 5308 BstYI r'GATC_y Cuts at: 1972 2638 3005 3390 3407 4859 4945 4957 5303 Size: 666 367 385 17 1452 86 12 346 Cuts at: 5303 1972 Size: 1977 Fragments arranged by size: 1977 1452 666 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 3817 Fragments arranged by size: 3817 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4569 Fragments arranged by size: 4569 683 56 BtsI GCAGTG_nn' Cuts at: 2824 3618 3638 2824 Size: 794 20 4494 Fragments arranged by size: 4494 794 20 ClaI AT'CG_AT Cuts at: 1554 2313 1554 Size: 759 4549 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5273 DraI TTT'AAA Cuts at: 2612 2612 Size: 5308 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4315 809 Size: 507 2999 1802 Fragments arranged by size: 2999 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2388 2551 2755 Size: 54 1425 50 275 18 558 163 204 Cuts at: 2755 3644 5273 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 558 275 204 163 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2755 1537 Size: 275 943 4090 Fragments arranged by size: 4090 943 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3238 1353 Size: 1885 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3895 4269 4415 387 Size: 1064 138 2306 374 146 1280 Fragments arranged by size: 2306 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2008 2239 2440 3371 3968 4755 5023 Size: 393 376 231 201 931 597 787 268 Cuts at: 5023 1239 Size: 1524 Fragments arranged by size: 1524 931 787 597 393 376 268 231 201 EcoO109I rG'GnC_Cy Cuts at: 2467 2510 2467 Size: 43 5265 EcoRI G'AATT_C Cuts at: 2617 2995 2617 Size: 378 4930 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2395 2395 Size: 5308 FspI TGC'GCA Cuts at: 3814 3814 Size: 5308 HaeII r_GCGC'y Cuts at: 1678 2496 2589 4455 1678 Size: 818 93 1866 2531 Fragments arranged by size: 2531 1866 818 93 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3949 3981 4023 4055 1373 Size: 32 135 32 2377 32 42 32 2626 Fragments arranged by size: 2626 2377 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2652 2898 669 Size: 988 281 714 246 3079 Fragments arranged by size: 3079 988 714 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5308 HpaI GTT'AAC Cuts at: 2898 2898 Size: 5308 KasI G'GCGC_C Cuts at: 2492 2492 Size: 5308 KpnI G_GTAC'C Cuts at: 2633 2633 Size: 5308 MfeI C'AATT_G Cuts at: 2907 2907 Size: 5308 MluI A'CGCG_T Cuts at: 5184 5184 Size: 5308 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4422 4606 1305 Size: 3117 184 2007 Fragments arranged by size: 3117 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3486 Fragments arranged by size: 3486 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3266 3625 3784 518 Size: 648 2100 359 159 2042 Fragments arranged by size: 2100 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2399 2541 2724 3401 4549 4794 1606 Size: 793 142 183 677 1148 245 2120 Fragments arranged by size: 2120 1148 793 677 245 183 142 NarI GG'CG_CC Cuts at: 2493 2493 Size: 5308 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 3817 Fragments arranged by size: 3817 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5308 NheI G'CTAG_C Cuts at: 1052 2588 1052 Size: 1536 3772 NotI GC'GGCC_GC Cuts at: 2755 2755 Size: 5308 NspI r_CATG'y Cuts at: 1132 1229 2464 2666 4211 1132 Size: 97 1235 202 1545 2229 Fragments arranged by size: 2229 1545 1235 202 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5308 PciI A'CATG_T Cuts at: 4207 4207 Size: 5308 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5308 PfoI T'CCnGG_A Cuts at: 2567 2567 Size: 5308 PmeI GTTT'AAAC Cuts at: 2612 2612 Size: 5308 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3377 3409 4890 4922 3377 Size: 32 1481 32 3763 Fragments arranged by size: 3763 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2467 2467 Size: 5308 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5308 PsiI TTA'TAA Cuts at: 2878 2878 Size: 5308 PspOMI G'GGCC_C Cuts at: 2509 2509 Size: 5308 PstI C_TGCA'G Cuts at: 830 2660 830 Size: 1830 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5308 SalI G'TCGA_C Cuts at: 1936 2650 1936 Size: 714 4594 SbfI CC_TGCA'GG Cuts at: 2660 2660 Size: 5308 ScaI AGT'ACT Cuts at: 3556 5268 3556 Size: 1712 3596 SfcI C'TryA_G Cuts at: 826 1045 2656 3791 4472 4663 826 Size: 219 1611 1135 681 191 1471 Fragments arranged by size: 1611 1471 1135 681 219 191 SfoI GGC'GCC Cuts at: 2494 2494 Size: 5308 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5308 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5308 SmaI CCC'GGG Cuts at: 1872 2635 1872 Size: 763 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3412 4313 4575 820 Size: 197 926 1469 901 262 1553 Fragments arranged by size: 1553 1469 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3099 493 Size: 2606 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5308 SphI G_CATG'C Cuts at: 1132 2464 2666 1132 Size: 1332 202 3774 Fragments arranged by size: 3774 1332 202 SspI AAT'ATT Cuts at: 5 52 3232 5 Size: 47 3180 2081 Fragments arranged by size: 3180 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5308 StyI C'CwwG_G Cuts at: 513 1065 2004 2418 2747 513 Size: 552 939 414 329 3074 Fragments arranged by size: 3074 939 552 414 329 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2355 3335 3352 3505 3690 4135 325 Size: 1255 775 980 17 153 185 445 1498 Fragments arranged by size: 1498 1255 980 775 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2124 2265 2278 Size: 274 80 33 51 1443 147 141 13 Cuts at: 2278 3554 5266 96 Size: 1276 1712 138 Fragments arranged by size: 1712 1443 1276 274 147 141 138 80 51 33 13 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5268 5300 5268 Size: 32 5276 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5308 XbaI T'CTAG_A Cuts at: 2644 2644 Size: 5308 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5308 XmaI C'CCGG_G Cuts at: 1870 2633 1870 Size: 763 4545 XmnI GAAnn'nnTTC Cuts at: 3437 3437 Size: 5308 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3116 276 Size: 53 83 186 936 1582 2468 Fragments arranged by size: 2468 1582 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI AscI AvrII BaeI BbvCI BfrBI BplI BsiWI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FseI FspAI NaeI NgoMIV NruI NsiI PacI PmlI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SexAI SfiI SrfI SwaI XcmI