################################## # NO CUT ENZYMES ( 126 enzymes) # ################################## AatII AccI AccIII AclI AcsI AcyI AflII AgeI AhdI Alw44I AocI ApaLI ApoI AscI AseI AsnI Asp700I AspEI AviII BbeI BbrPI BbsI BbvCI BcgI BfrI BglII BpuAI BsaAI BsaBI BsaHI BsaI BseAI BseRI BsiEI BsiWI BsmBI BsmI Bsp1407I BspDI BspEI BspHI BsrBI BsrFI BsrGI BssHII BssSI Bst1107I BstBI BstEII BstPI BstXI BstZ17I Bsu36I Cfr10I ClaI CpoI DraI DraIII EagI Eam1105I EarI EclXI Eco52I Eco81I EcoO65I EcoRI EcoRV EcoT22I FseI FspI HgaI Hin1I HincII HindII HindIII HpaI HphI KasI Ksp632I KspI MamI MboII MfeI MluI MroI MunI NaeI NarI NdeI NgoMIV NotI NruI NsiI NspV PacI PaeR7I PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RcaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfoI SfuI SgrAI SmlI SnaBI SpeI Sse8387I SspBI SspI SwaI XbaI XhoI XmnI ################################### # ONE CUT ENZYMES ( 40 enzymes) # ################################### Acc65I 711 GGTACC G^GTACC AfaI 712 GTAC GT^AC AflIII 183 ACATGT A^C[GA][CT]GT Asp718I 711 GGTACC G^GTACC AspHI 828 GTGCTC G[AT]GC[AT]^C AvrII 153 CCTAGG C^CTAGG BalI 245 TGGCCA TGG^CCA BamHI 650 GGATCC G^GATCC BciVI 1124 GTATCC GTATCC(6/5) BclI 641 TGATCA T^GATCA BlnI 153 CCTAGG C^CTAGG BlpI 748 GCTGAGC GC^T[ACGT]AGC Bpu1102I 748 GCTGAGC GC^T[ACGT]AGC BsaWI 824 TCCGGT [AT]^CCGG[AT] BsgI 144 GTGCAG GTGCAG(16/14) BsiHKAI 828 GTGCTC G[AT]GC[AT]^C BsmAI 884 GAGAC GTCTC(1/5) BspLU11I 183 ACATGT A^CATGT CelII 748 GCTGAGC GC^T[ACGT]AGC DrdI 520 GACAAGCTCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC DsaI 833 CCATGG C^C[GA][CT]GG EaeI 245 TGGCCA [CT]^GGCC[GA] EcoNI 285 CCTGGAGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG FbaI 641 TGATCA T^GATCA KpnI 711 GGTACC GGTAC^C MaeII 302 ACGT A^CGT MluNI 245 TGGCCA TGG^CCA MscI 245 TGGCCA TGG^CCA MseI 236 TTAA T^TAA NcoI 833 CCATGG C^CATGG NheI 678 GCTAGC G^CTAGC PpuMI 494 GGGACCC [GA]G^G[AT]CC[CT] RsaI 712 GTAC GT^AC SfiI 246 GGCCAAGGAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SmaI 544 CCCGGG CCC^GGG SphI 791 GCATGC GCATG^C TfiI 663 GATTC G^A[AT]TC Tru9I 236 TTAA T^TAA XmaCI 544 CCCGGG C^CCGGG XmaI 544 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 30 enzymes) # ################################### AccII 706 CGCG CG^CG 881 CGCG Aor51HI 752 AGCGCT AGC^GCT 844 AGCGCT ApaI 102 GGGCCC GGGCC^C 548 GGGCCC AspI 220 GACATCGTC GAC[ACGT]^[ACGT][ACGT]GTC 1101 GACCATGTC AvaI 544 CCCGGG C^[CT]CG[GA]G 1087 CTCGGG BfaI 154 CTAG C^TAG 679 CTAG BglI 247 GCCAAGGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 550 GCCCTCCAGGC BsoBI 544 CCCGGG C^[CT]CG[GA]G 1087 CTCGGG BspMI 14 ACCTGC ACCTGC(4/8) 1075 ACCTGC BstAPI 21 GCAGTGGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 132 GCAAGCATTGC BstUI 706 CGCG CG^CG 881 CGCG BstYI 582 GGATCT [GA]^GATC[CT] 650 GGATCC Eco47III 752 AGCGCT AGC^GCT 844 AGCGCT FauI 492 GCGGG CCCGC(4/6) 724 GCGGG HaeII 752 AGCGCT [GA]GCGC^[CT] 844 AGCGCT MaeI 154 CTAG C^TAG 679 CTAG MaeIII 9 GTCAC ^GT[ACGT]AC 913 GTGAC MflI 582 GGATCT [GA]^GATC[CT] 650 GGATCC MspA1I 802 CAGCTG C[AC]G^C[GT]G 1033 CAGCTG MvnI 706 CGCG CG^CG 881 CGCG PflFI 220 GACATCGTC GAC[ACGT]^[ACGT][ACGT]GTC 1101 GACCATGTC PstI 19 CTGCAG CTGCA^G 630 CTGCAG PvuII 802 CAGCTG CAG^CTG 1033 CAGCTG StuI 562 AGGCCT AGG^CCT 968 AGGCCT TaqI 371 TCGA T^CGA 773 TCGA Tsp45I 9 GTCAC ^GT[GC]AC 913 GTGAC Tsp509I 599 AATT ^AATT 1010 AATT Tth111I 220 GACATCGTC GAC[ACGT]^[ACGT][ACGT]GTC 1101 GACCATGTC TthHB8I 371 TCGA T^CGA 773 TCGA XhoII 582 GGATCT [GA]^GATC[CT] 650 GGATCC ######################################### # 3 OR MORE CUT ENZYMES ( 62 enzymes) # ######################################### AciI AluI AlwI AlwNI AvaII BanI BanII BbvI BcnI BmyI BpmI BsaJI BsiYI BslI BsmFI Bsp1286I BsrDI BsrI BssKI BstF5I BstNI Cac8I CfoI Cfr13I DdeI DpnII DraII EcoO109I EcoRII EcoT14I Fnu4HI FokI HaeIII HapII HhaI HinP1I HinfI HpaII ItaI MboI MnlI MslI MspI MvaI MwoI NciI NdeII NlaIII NlaIV NspI PflMI PleI Sau3AI Sau96I ScrFI SfaNI SfcI StyI TseI TspRI Van91I XcmI