(Circular) (Six-base) MAPSORT of: pFN21AE4363.seq Check: 8178 from: 1 to: 5848 pFN21AE4363 5848 bp With 182 enzymes: SgfI * March 28, 2012 13:31 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2028 3089 2028 Size: 1061 4787 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3658 278 Size: 53 83 186 936 2122 2468 Fragments arranged by size: 2468 2122 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3191 1937 Size: 1254 4594 Acc65I G'GTAC_C Cuts at: 2716 3169 2716 Size: 453 5395 AclI AA'CG_TT Cuts at: 1563 3975 4348 1563 Size: 2412 373 3063 Fragments arranged by size: 3063 2412 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3911 5295 5563 1632 Size: 2279 1384 268 1917 Fragments arranged by size: 2279 1917 1384 268 AfeI AGC'GCT Cuts at: 2759 2851 2759 Size: 92 5756 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5651 AflIII A'CryG_T Cuts at: 1203 2188 4747 5724 1203 Size: 985 2559 977 1327 Fragments arranged by size: 2559 1327 985 977 AgeI A'CCGG_T Cuts at: 4679 4679 Size: 5848 AhdI GACnn_n'nnGTC Cuts at: 4577 4577 Size: 5848 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5848 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2164 2363 2432 1184 Size: 980 199 69 4600 Fragments arranged by size: 4600 980 199 69 AlwNI CAG_nnn'CTG Cuts at: 2032 2069 2749 3043 3063 3591 5163 2032 Size: 37 680 294 20 528 1572 2717 Fragments arranged by size: 2717 1572 680 528 294 37 20 ApaI G_GGCC'C Cuts at: 2111 2557 2111 Size: 446 5402 ApaLI G'TGCA_C Cuts at: 3905 5061 3905 Size: 1156 4692 ApoI r'AATT_y Cuts at: 1493 3157 3383 3535 5644 1493 Size: 1664 226 152 2109 1697 Fragments arranged by size: 2109 1697 1664 226 152 AseI AT'TA_AT Cuts at: 160 1032 4402 160 Size: 872 3370 1606 Fragments arranged by size: 3370 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5848 AvaI C'yCGr_G Cuts at: 1870 1943 2549 3092 3173 1870 Size: 73 606 543 81 4545 Fragments arranged by size: 4545 606 543 81 73 AvrII C'CTAG_G Cuts at: 2158 2158 Size: 5848 BamHI G'GATC_C Cuts at: 2655 3178 3545 2655 Size: 523 367 4958 Fragments arranged by size: 4958 523 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2121 2716 3052 3169 4624 Size: 325 217 627 334 595 336 117 1455 Cuts at: 4624 618 Size: 1842 Fragments arranged by size: 1842 1455 627 595 336 334 325 217 117 BanII G_rGCy'C Cuts at: 1391 2111 2158 2557 2644 3167 1391 Size: 720 47 399 87 523 4072 Fragments arranged by size: 4072 720 523 399 87 47 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4877 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4039 4073 1903 Size: 34 2102 34 3678 Fragments arranged by size: 3678 2102 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3140 3740 4950 3140 Size: 600 1210 4038 Fragments arranged by size: 4038 1210 600 BclI T'GATC_A Cuts at: 1548 2646 1548 Size: 1098 4750 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2258 2561 4459 Size: 107 122 71 631 605 586 303 1898 Cuts at: 4459 136 Size: 1525 Fragments arranged by size: 1898 1525 631 605 586 303 122 107 71 BglII A'GATC_T Cuts at: 5843 5843 Size: 5848 BlpI GC'TnA_GC Cuts at: 2754 3265 2754 Size: 511 5337 Bme1580I G_kGCm'C Cuts at: 1790 2104 2111 2557 3057 3909 5065 1790 Size: 314 7 446 500 852 1156 2573 Fragments arranged by size: 2573 1156 852 500 446 314 7 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5848 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2329 3579 4537 Size: 416 53 83 399 24 912 1250 958 Cuts at: 4537 442 Size: 1753 Fragments arranged by size: 1753 1250 958 912 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 2687 1056 Size: 1631 4217 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2779 2779 Size: 5848 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2153 2312 2543 2954 3107 4508 1239 Size: 914 159 231 411 153 1401 2579 Fragments arranged by size: 2579 1401 914 411 231 159 153 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3973 4838 5136 3973 Size: 865 298 4685 Fragments arranged by size: 4685 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4511 882 Size: 617 3012 2219 Fragments arranged by size: 3012 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3639 493 Size: 711 2435 2702 Fragments arranged by size: 2702 2435 711 BsaBI GATnn'nnATC Cuts at: 5842 5842 Size: 5848 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3655 4037 4672 275 Size: 53 83 186 936 2122 382 635 1451 Fragments arranged by size: 2122 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2829 4281 4679 4953 5100 5511 2829 Size: 1452 398 274 147 411 3166 Fragments arranged by size: 3166 1452 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2285 2315 1178 Size: 30 165 30 882 30 4711 Fragments arranged by size: 4711 882 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5848 BseYI C'CCAG_C Cuts at: 2788 2805 3036 3063 5051 5547 2788 Size: 17 231 27 1988 496 3089 Fragments arranged by size: 3089 1988 496 231 27 17 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2170 1163 Size: 573 434 4841 Fragments arranged by size: 4841 573 434 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3298 4059 5087 1540 Size: 275 186 1297 761 1028 2301 Fragments arranged by size: 2301 1297 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2837 3909 3994 5065 2837 Size: 1072 85 1071 3620 Fragments arranged by size: 3620 1072 1071 85 BsmI GAATG_Cn' Cuts at: 3359 3452 3359 Size: 93 5755 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2104 2111 2158 2557 2644 2837 3057 Size: 399 314 7 47 399 87 193 220 Cuts at: 3057 3167 3909 3994 5065 1391 Size: 110 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 399 314 220 193 110 87 85 47 7 BspEI T'CCGG_A Cuts at: 5511 5511 Size: 5848 BspHI T'CATG_A Cuts at: 3737 5467 3737 Size: 1730 4118 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2028 3089 3203 844 Size: 1184 1061 114 3489 Fragments arranged by size: 3489 1184 1061 114 BsrBI CCG'CTC Cuts at: 1394 1823 3735 1394 Size: 429 1912 3507 Fragments arranged by size: 3507 1912 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2141 2164 2970 4343 4517 65 Size: 1183 893 23 806 1373 174 1396 Fragments arranged by size: 1396 1373 1183 893 806 174 23 BsrFI r'CCGG_y Cuts at: 1700 1810 4492 4679 1700 Size: 110 2682 187 2869 Fragments arranged by size: 2869 2682 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5848 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5848 BssSI C'ACGA_G Cuts at: 3908 4920 3908 Size: 1012 4836 BstAPI GCAn_nnn'nTGC Cuts at: 794 2032 2143 794 Size: 1238 111 4499 Fragments arranged by size: 4499 1238 111 BstBI TT'CG_AA Cuts at: 4652 4652 Size: 5848 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5848 BstYI r'GATC_y Cuts at: 1972 2587 2655 3178 3545 3930 3947 5399 5485 Size: 615 68 523 367 385 17 1452 86 Cuts at: 5485 5497 5843 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 615 523 385 367 346 86 68 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2838 513 Size: 552 939 834 3523 Fragments arranged by size: 3523 939 834 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5109 Fragments arranged by size: 5109 683 56 BtsI GCAGTG_nn' Cuts at: 2033 2067 2153 2700 3364 4158 4178 2033 Size: 34 86 547 664 794 20 3703 Fragments arranged by size: 3703 794 664 547 86 34 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5848 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5813 DraI TTT'AAA Cuts at: 3152 3152 Size: 5848 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2531 4855 809 Size: 507 1215 2324 1802 Fragments arranged by size: 2324 1802 1215 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2250 3295 4184 Size: 54 1425 50 275 18 420 1045 889 Cuts at: 4184 5813 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1045 889 420 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3295 1537 Size: 275 1483 4090 Fragments arranged by size: 4090 1483 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3778 1353 Size: 2425 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4435 4809 4955 387 Size: 1064 138 2846 374 146 1280 Fragments arranged by size: 2846 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2153 2312 2543 2954 3107 3911 4508 Size: 393 521 159 231 411 153 804 597 Cuts at: 4508 5295 5563 1239 Size: 787 268 1524 Fragments arranged by size: 1524 804 787 597 521 411 393 268 231 159 153 EcoNI CCTnn'n_nnAGG Cuts at: 2294 2294 Size: 5848 EcoO109I rG'GnC_Cy Cuts at: 2107 2108 2500 2553 2554 3088 2107 Size: 1 392 53 1 534 4867 Fragments arranged by size: 4867 534 392 53 1 1 EcoRI G'AATT_C Cuts at: 3157 3535 3157 Size: 378 5470 FspI TGC'GCA Cuts at: 4354 4354 Size: 5848 HaeII r_GCGC'y Cuts at: 1678 2761 2853 4995 1678 Size: 1083 92 2142 2531 Fragments arranged by size: 2531 2142 1083 92 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4489 4521 4563 4595 1373 Size: 32 135 32 2917 32 42 32 2626 Fragments arranged by size: 2917 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3192 3438 669 Size: 988 281 1254 246 3079 Fragments arranged by size: 3079 1254 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5848 HpaI GTT'AAC Cuts at: 3438 3438 Size: 5848 KpnI G_GTAC'C Cuts at: 2720 3173 2720 Size: 453 5395 MfeI C'AATT_G Cuts at: 3447 3447 Size: 5848 MluI A'CGCG_T Cuts at: 5724 5724 Size: 5848 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4962 5146 1305 Size: 3657 184 2007 Fragments arranged by size: 3657 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2252 10 Size: 54 1425 343 420 3606 Fragments arranged by size: 3606 1425 420 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2146 2414 2915 3806 4165 4324 518 Size: 648 980 268 501 891 359 159 2042 Fragments arranged by size: 2042 980 891 648 501 359 268 159 MspA1I CmG'CkG Cuts at: 1606 2809 3040 3264 3941 5089 5334 1606 Size: 1203 231 224 677 1148 245 2120 Fragments arranged by size: 2120 1203 1148 677 245 231 224 NcoI C'CATG_G Cuts at: 513 1065 2004 2838 513 Size: 552 939 834 3523 Fragments arranged by size: 3523 939 834 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5848 NheI G'CTAG_C Cuts at: 1052 2683 1052 Size: 1631 4217 NotI GC'GGCC_GC Cuts at: 3295 3295 Size: 5848 NspI r_CATG'y Cuts at: 1132 1229 2192 2800 2914 3206 4751 1132 Size: 97 963 608 114 292 1545 2229 Fragments arranged by size: 2229 1545 963 608 292 114 97 PasI CC'CwG_GG Cuts at: 1348 2104 3057 1348 Size: 756 953 4139 Fragments arranged by size: 4139 953 756 PciI A'CATG_T Cuts at: 2188 4747 2188 Size: 2559 3289 PflMI CCAn_nnn'nTGG Cuts at: 1108 2131 2749 3063 1108 Size: 1023 618 314 3893 Fragments arranged by size: 3893 1023 618 314 PfoI T'CCnGG_A Cuts at: 2268 2289 2268 Size: 21 5827 PmeI GTTT'AAAC Cuts at: 3152 3152 Size: 5848 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3917 3949 5430 5462 3917 Size: 32 1481 32 4303 Fragments arranged by size: 4303 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2500 2500 Size: 5848 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5848 PsiI TTA'TAA Cuts at: 3418 3418 Size: 5848 PspOMI G'GGCC_C Cuts at: 2107 2553 2107 Size: 446 5402 PstI C_TGCA'G Cuts at: 830 2028 2639 3200 830 Size: 1198 611 561 3478 Fragments arranged by size: 3478 1198 611 561 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5848 PvuII CAG'CTG Cuts at: 2809 3040 2809 Size: 231 5617 SalI G'TCGA_C Cuts at: 1936 3190 1936 Size: 1254 4594 SanDI GG'GwC_CC Cuts at: 2500 2500 Size: 5848 SbfI CC_TGCA'GG Cuts at: 3200 3200 Size: 5848 ScaI AGT'ACT Cuts at: 4096 5808 4096 Size: 1712 4136 SfcI C'TryA_G Cuts at: 826 1045 2024 2171 2635 3196 4331 5012 5203 Size: 219 979 147 464 561 1135 681 191 Cuts at: 5203 826 Size: 1471 Fragments arranged by size: 1471 1135 979 681 561 464 219 191 147 SfiI GGCCn_nnn'nGGCC Cuts at: 2258 2258 Size: 5848 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5848 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5848 SmaI CCC'GGG Cuts at: 1872 2551 3175 1872 Size: 679 624 4545 Fragments arranged by size: 4545 679 624 SmlI C'TyrA_G Cuts at: 820 1017 1943 3952 4853 5115 820 Size: 197 926 2009 901 262 1553 Fragments arranged by size: 2009 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3639 493 Size: 3146 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5848 SphI G_CATG'C Cuts at: 1132 2800 3206 1132 Size: 1668 406 3774 Fragments arranged by size: 3774 1668 406 SspI AAT'ATT Cuts at: 5 52 3772 5 Size: 47 3720 2081 Fragments arranged by size: 3720 2081 47 StuI AGG'CCT Cuts at: 1517 2569 2975 1517 Size: 1052 406 4390 Fragments arranged by size: 4390 1052 406 StyI C'CwwG_G Cuts at: 513 1065 2004 2091 2158 2253 2673 2838 2858 Size: 552 939 87 67 95 420 165 20 Cuts at: 2858 3287 513 Size: 429 3074 Fragments arranged by size: 3074 939 552 429 420 165 95 87 67 20 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2939 3875 3892 4045 4230 4675 325 Size: 1255 1359 936 17 153 185 445 1498 Fragments arranged by size: 1498 1359 1255 936 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4094 5806 96 Size: 274 80 33 51 1443 2117 1712 138 Fragments arranged by size: 2117 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2285 2317 5808 5840 2285 Size: 32 3491 32 2293 Fragments arranged by size: 3491 2293 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2228 3109 1316 Size: 912 881 4055 Fragments arranged by size: 4055 912 881 XbaI T'CTAG_A Cuts at: 3184 3184 Size: 5848 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2089 2567 2978 2089 Size: 478 411 4959 Fragments arranged by size: 4959 478 411 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5848 XmaI C'CCGG_G Cuts at: 1870 2549 3173 1870 Size: 679 624 4545 Fragments arranged by size: 4545 679 624 XmnI GAAnn'nnTTC Cuts at: 3977 3977 Size: 5848 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3656 276 Size: 53 83 186 936 2122 2468 Fragments arranged by size: 2468 2122 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SalI SanDI SbfI ScaI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI AscI BaeI BbeI BbvCI BfrBI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspXI PsrI RsrII SacI SacII SapI SexAI SfoI SrfI SwaI