(Linear) (Six-base) MAPSORT of: KIEE4363.seq Check: 3911 from: 1 to: 1143 KIEE4363.orf With 182 enzymes: SgfI * March 22, 2012 17:49 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 23 1084 1143 Size: 23 1061 59 Fragments arranged by size: 1061 59 23 Acc65I G'GTAC_C Cuts at: 0 711 1143 Size: 711 432 AfeI AGC'GCT Cuts at: 0 754 846 1143 Size: 754 92 297 Fragments arranged by size: 754 297 92 AflIII A'CryG_T Cuts at: 0 183 1143 Size: 183 960 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 159 358 427 1143 Size: 159 199 69 716 Fragments arranged by size: 716 199 159 69 AlwNI CAG_nnn'CTG Cuts at: 0 27 64 744 1038 1058 1143 Size: 27 37 680 294 20 85 Fragments arranged by size: 680 294 85 37 27 20 ApaI G_GGCC'C Cuts at: 0 106 552 1143 Size: 106 446 591 Fragments arranged by size: 591 446 106 AvaI C'yCGr_G Cuts at: 0 544 1087 1143 Size: 544 543 56 Fragments arranged by size: 544 543 56 AvrII C'CTAG_G Cuts at: 0 153 1143 Size: 153 990 BamHI G'GATC_C Cuts at: 0 650 1143 Size: 650 493 BanI G'GyrC_C Cuts at: 0 116 711 1047 1143 Size: 116 595 336 96 Fragments arranged by size: 595 336 116 96 BanII G_rGCy'C Cuts at: 0 106 153 552 639 1143 Size: 106 47 399 87 504 Fragments arranged by size: 504 399 106 87 47 BciVI GTATCCnnnnn_n' Cuts at: 0 1135 1143 Size: 1135 8 BclI T'GATC_A Cuts at: 0 641 1143 Size: 641 502 BglI GCCn_nnn'nGGC Cuts at: 0 253 556 1143 Size: 253 303 587 Fragments arranged by size: 587 303 253 BlpI GC'TnA_GC Cuts at: 0 749 1143 Size: 749 394 Bme1580I G_kGCm'C Cuts at: 0 99 106 552 1052 1143 Size: 99 7 446 500 91 Fragments arranged by size: 500 446 99 91 7 BmrI ACTGGGnnnn_n' Cuts at: 0 324 1143 Size: 324 819 BmtI G_CTAG'C Cuts at: 0 682 1143 Size: 682 461 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 774 1143 Size: 774 369 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 148 307 538 949 1102 1143 Size: 148 159 231 411 153 41 Fragments arranged by size: 411 231 159 153 148 41 BsaWI w'CCGG_w Cuts at: 0 824 1143 Size: 824 319 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 280 310 1143 Size: 280 30 833 Fragments arranged by size: 833 280 30 BseYI C'CCAG_C Cuts at: 0 783 800 1031 1058 1143 Size: 783 17 231 27 85 Fragments arranged by size: 783 231 85 27 17 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 165 1143 Size: 165 978 BsiHKAI G_wGCw'C Cuts at: 0 832 1143 Size: 832 311 Bsp1286I G_dGCh'C Cuts at: 0 99 106 153 552 639 832 1052 1143 Size: 99 7 47 399 87 193 220 91 Fragments arranged by size: 399 220 193 99 91 87 47 7 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 23 1084 1143 Size: 23 1061 59 Fragments arranged by size: 1061 59 23 BsrDI GCAATG_nn' Cuts at: 0 136 159 965 1143 Size: 136 23 806 178 Fragments arranged by size: 806 178 136 23 BstAPI GCAn_nnn'nTGC Cuts at: 0 27 138 1143 Size: 27 111 1005 Fragments arranged by size: 1005 111 27 BstYI r'GATC_y Cuts at: 0 582 650 1143 Size: 582 68 493 Fragments arranged by size: 582 493 68 BtgI C'CryG_G Cuts at: 0 833 1143 Size: 833 310 BtsI GCAGTG_nn' Cuts at: 0 28 62 148 695 1143 Size: 28 34 86 547 448 Fragments arranged by size: 547 448 86 34 28 DrdI GACnn_nn'nnGTC Cuts at: 0 526 1143 Size: 526 617 EaeI y'GGCC_r Cuts at: 0 245 1143 Size: 245 898 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 148 307 538 949 1102 1143 Size: 148 159 231 411 153 41 Fragments arranged by size: 411 231 159 153 148 41 EcoNI CCTnn'n_nnAGG Cuts at: 0 289 1143 Size: 289 854 EcoO109I rG'GnC_Cy Cuts at: 0 102 103 495 548 549 1083 1143 Size: 102 1 392 53 1 534 60 Fragments arranged by size: 534 392 102 60 53 1 1 HaeII r_GCGC'y Cuts at: 0 756 848 1143 Size: 756 92 295 Fragments arranged by size: 756 295 92 KpnI G_GTAC'C Cuts at: 0 715 1143 Size: 715 428 MscI TGG'CCA Cuts at: 0 247 1143 Size: 247 896 MslI CAynn'nnrTG Cuts at: 0 141 409 910 1143 Size: 141 268 501 233 Fragments arranged by size: 501 268 233 141 MspA1I CmG'CkG Cuts at: 0 804 1035 1143 Size: 804 231 108 Fragments arranged by size: 804 231 108 NcoI C'CATG_G Cuts at: 0 833 1143 Size: 833 310 NheI G'CTAG_C Cuts at: 0 678 1143 Size: 678 465 NspI r_CATG'y Cuts at: 0 187 795 909 1143 Size: 187 608 114 234 Fragments arranged by size: 608 234 187 114 PasI CC'CwG_GG Cuts at: 0 99 1052 1143 Size: 99 953 91 Fragments arranged by size: 953 99 91 PciI A'CATG_T Cuts at: 0 183 1143 Size: 183 960 PflMI CCAn_nnn'nTGG Cuts at: 0 126 744 1058 1143 Size: 126 618 314 85 Fragments arranged by size: 618 314 126 85 PfoI T'CCnGG_A Cuts at: 0 263 284 1143 Size: 263 21 859 Fragments arranged by size: 859 263 21 PpuMI rG'GwC_Cy Cuts at: 0 495 1143 Size: 495 648 PspOMI G'GGCC_C Cuts at: 0 102 548 1143 Size: 102 446 595 Fragments arranged by size: 595 446 102 PstI C_TGCA'G Cuts at: 0 23 634 1143 Size: 23 611 509 Fragments arranged by size: 611 509 23 PvuII CAG'CTG Cuts at: 0 804 1035 1143 Size: 804 231 108 Fragments arranged by size: 804 231 108 SanDI GG'GwC_CC Cuts at: 0 495 1143 Size: 495 648 SfcI C'TryA_G Cuts at: 0 19 166 630 1143 Size: 19 147 464 513 Fragments arranged by size: 513 464 147 19 SfiI GGCCn_nnn'nGGCC Cuts at: 0 253 1143 Size: 253 890 SmaI CCC'GGG Cuts at: 0 546 1143 Size: 546 597 SphI G_CATG'C Cuts at: 0 795 1143 Size: 795 348 StuI AGG'CCT Cuts at: 0 564 970 1143 Size: 564 406 173 Fragments arranged by size: 564 406 173 StyI C'CwwG_G Cuts at: 0 86 153 248 668 833 853 1143 Size: 86 67 95 420 165 20 290 Fragments arranged by size: 420 290 165 95 86 67 20 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 934 1143 Size: 934 209 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 280 312 1143 Size: 280 32 831 Fragments arranged by size: 831 280 32 Tth111I GACn'n_nGTC Cuts at: 0 223 1104 1143 Size: 223 881 39 Fragments arranged by size: 881 223 39 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 84 562 973 1143 Size: 84 478 411 170 Fragments arranged by size: 478 411 170 84 XmaI C'CCGG_G Cuts at: 0 544 1143 Size: 544 599 Enzymes that do cut: AarI Acc65I AfeI AflIII AlfI AlwNI ApaI AvaI AvrII BamHI BanI BanII BciVI BclI BglI BlpI Bme1580I BmrI BmtI BplI BpmI BsaWI BsaXI BseYI BsgI BsiHKAI Bsp1286I BspMI BsrDI BstAPI BstYI BtgI BtsI DrdI EaeI Eco57MI EcoNI EcoO109I HaeII KpnI MscI MslI MspA1I NcoI NheI NspI PasI PciI PflMI PfoI PpuMI PspOMI PstI PvuII SanDI SfcI SfiI SmaI SphI StuI StyI TaqII TstI Tth111I XcmI XmaI Enzymes that do not cut: AatII AccI AclI AcuI AflII AgeI AhdI AleI AloI ApaLI ApoI AscI AseI AsiSI BaeI BbeI BbsI BbvCI BcgI BfrBI BglII BmgBI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BseRI BsiEI BsiWI BsmI BsmBI BspEI BspHI BsrBI BsrFI BsrGI BssHII BssSI BstBI BstEII BstXI BstZ17I Bsu36I BtgZI ClaI CspCI DraI DraIII EagI EarI EciI EcoICRI EcoRI EcoRV FalI FseI FspI FspAI Hin4I HincII HindIII HpaI KasI MfeI MluI MmeI NaeI NarI NdeI NgoMIV NotI NruI NsiI PacI PmeI PmlI PpiI PshAI PsiI PspXI PsrI PvuI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfoI SgfI SgrAI SmlI SnaBI SpeI SrfI SspI SwaI TatI XbaI XhoI XmnI ZraI