################################## # NO CUT ENZYMES ( 180 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AflII AflIII AgeI AhdI Alw44I AlwI AlwNI AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AspI AvaI AviII AvrII BalI BamHI BanI BbeI BbrPI BbvCI BcgI BciVI BclI BcnI BfaI BfrI BglI BglII BlnI BsaAI BsaBI BsaI BsaWI BseAI BsgI BsiEI BsiWI BsoBI BspDI BspEI BspHI BspLU11I BsrBI BsrDI BsrFI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstYI BstZ17I Bsu36I CfoI Cfr10I ClaI CpoI DpnII DraI DraII DraIII DrdI EaeI EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRI EcoRV EcoT22I FauI FbaI FseI FspI HaeII HaeIII HapII HhaI HinP1I HincII HindII HindIII HinfI HpaI HpaII KasI KpnI Ksp632I KspI MaeI MaeII MamI MboI MfeI MflI MluI MluNI MroI MscI MseI MspI MunI NaeI NarI NciI NdeI NdeII NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PflMI PinAI PleI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI RcaI RsrII SacII SalI SapI Sau3AI SbfI ScaI SexAI SfcI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspI StuI SwaI TfiI Tru9I Tth111I Van91I XbaI XhoI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 37 enzymes) # ################################### AccII 56 CGCG CG^CG AciI 348 CCGC CCGC(-3/-1) AcyI 3 GGCGTC G[GA]^CG[CT]C AfaI 453 GTAC GT^AC BbsI 112 GAAGAC GAAGAC(2/6) BpuAI 112 GAAGAC GAAGAC(2/6) BsaHI 3 GGCGTC G[GA]^CG[CT]C BsiYI 42 CCCTGAGCAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BslI 42 CCCTGAGCAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BsmBI 52 GAGACG CGTCTC(1/5) BsmI 305 GCATTC GAATGC(1/-1) Bsp1407I 452 TGTACA T^GTACA BspMI 146 ACCTGC ACCTGC(4/8) BsrGI 452 TGTACA T^GTACA BsrI 429 ACTGG ACTGG(1/-1) BstF5I 101 GGATG GGATG(2/0) BstUI 56 CGCG CG^CG BstXI 164 CCAAGACTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG DsaI 441 CCATGG C^C[GA][CT]GG EcoT14I 441 CCATGG C^C[AT][AT]GG FokI 101 GGATG GGATG(9/13) Hin1I 3 GGCGTC G[GA]^CG[CT]C HphI 285 TCACC GGTGA(8/7) MaeIII 87 GTCAC ^GT[ACGT]AC MboII 112 GAAGA GAAGA(8/7) MslI 267 CATTGAGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MvnI 56 CGCG CG^CG NcoI 441 CCATGG C^CATGG NspI 65 ACATGC [GA]CATG^[CT] RsaI 453 GTAC GT^AC SfaNI 273 GATGC GCATC(5/9) SmlI 220 CTCAAG C^T[CT][GA]AG SspBI 452 TGTACA T^GTACA StyI 441 CCATGG C^C[AT][AT]GG Tsp45I 87 GTCAC ^GT[GC]AC TspRI 363 CACTG CA[GC]TG[ACGT][ACGT]^ XcmI 62 CCAACATGCTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 26 enzymes) # ################################### AcsI 244 GAATTT [GA]^AATT[CT] 330 AAATTC ApoI 244 GAATTT [GA]^AATT[CT] 330 AAATTC AvaII 39 GGACC G^G[AT]CC 138 GGACC BanII 76 GAGCTC G[GA]GC[CT]^C 217 GAGCTC BlpI 214 GCTGAGC GC^T[ACGT]AGC 318 GCTAAGC BpmI 73 CTGGAG CTGGAG(16/14) 430 CTGGAG Bpu1102I 214 GCTGAGC GC^T[ACGT]AGC 318 GCTAAGC BsmAI 52 GAGAC GTCTC(1/5) 259 GAGAC BsmFI 137 GGGAC GGGAC(10/14) 338 GTCCC BssKI 188 CCAGG ^CC[ACGT]GG 314 CCTGG BstNI 188 CCAGG CC^[AT]GG 314 CCTGG Cac8I 29 GCGAGC GC[ACGT]^[ACGT]GC 78 GCTCGC CelII 214 GCTGAGC GC^T[ACGT]AGC 318 GCTAAGC Cfr13I 39 GGACC G^G[ACGT]CC 138 GGACC EcoRII 188 CCAGG ^CC[AT]GG 314 CCTGG HgaI 54 GACGC GACGC(5/10) 4 GCGTC MspA1I 158 CAGCTG C[AC]G^C[GT]G 300 CAGCTG MvaI 188 CCAGG CC^[AT]GG 314 CCTGG MwoI 48 GCAGGAGACGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC 69 GCTGCTGGAGC PvuII 158 CAGCTG CAG^CTG 300 CAGCTG SacI 76 GAGCTC GAGCT^C 217 GAGCTC Sau96I 39 GGACC G^G[ACGT]CC 138 GGACC ScrFI 188 CCAGG CC^[ACGT]GG 314 CCTGG TaqI 14 TCGA T^CGA 405 TCGA Tsp509I 245 AATT ^AATT 331 AATT TthHB8I 14 TCGA T^CGA 405 TCGA ######################################### # 3 OR MORE CUT ENZYMES ( 15 enzymes) # ######################################### AluI AspHI BbvI BmyI BsaJI BseRI BsiHKAI Bsp1286I DdeI Fnu4HI ItaI MnlI NlaIII NlaIV TseI