################################## # NO CUT ENZYMES ( 90 enzymes) # ################################## AatII AccI AccIII AclI AcsI AflII AflIII AgeI Aor51HI ApoI AscI AseI AsnI AviII AvrII BalI BbsI BbvCI BcgI BciVI BclI BfrI BglII BlnI BlpI Bpu1102I BpuAI BsaBI BsaWI BseAI BsiWI Bsp1407I BspEI BspHI BspLU11I BsrGI Bst1107I BstBI BstZ17I CelII CpoI DrdI Eco47III EcoRI EcoRV FbaI FseI FspI HgaI HindIII HpaI MamI MfeI MluI MluNI MroI MscI MslI MunI NcoI NdeI NheI NotI NruI NspV PacI PflMI PinAI PmeI PshBI Psp1406I PvuI RcaI RsrII SacI SalI SapI SbfI SfiI SfuI SgrAI SnaBI SpeI Sse8387I SspBI SspI SwaI Tsp509I Van91I XbaI ################################### # ONE CUT ENZYMES ( 65 enzymes) # ################################### Acc65I 362 GGTACC G^GTACC AcyI 257 GGCGCC G[GA]^CG[CT]C AhdI 496 GACACGAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Alw44I 1101 GTGCAC G^TGCAC AlwNI 1369 CAGTACCTG CAG[ACGT][ACGT][ACGT]^CTG ApaI 1535 GGGCCC GGGCC^C ApaLI 1101 GTGCAC G^TGCAC Asp700I 1050 GAATGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC Asp718I 362 GGTACC G^GTACC AspEI 496 GACACGAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AspHI 1101 GTGCAC G[AT]GC[AT]^C BamHI 911 GGATCC G^GATCC BbeI 257 GGCGCC GGCGC^C BbrPI 1033 CACGTG CAC^GTG BfaI 184 CTAG C^TAG BsaAI 1033 CACGTG [CT]AC^GT[GA] BsaHI 257 GGCGCC G[GA]^CG[CT]C BsaI 849 GGTCTC GGTCTC(1/5) BsgI 1118 GTGCAG GTGCAG(16/14) BsiEI 74 CGGCCG CG[GA][CT]^CG BsiHKAI 1101 GTGCAC G[AT]GC[AT]^C BsmBI 930 CGTCTC CGTCTC(1/5) BsmI 1050 GAATGC GAATGC(1/-1) BspDI 643 ATCGAT AT^CGAT BsrBI 1204 GAGCGG CCGCTC(-3/-3) BsrDI 593 GCAATG GCAATG(2/0) BssHII 433 GCGCGC G^CGCGC BssSI 498 CACGAG CACGAG(-5/-1) ClaI 643 ATCGAT AT^CGAT DraI 340 TTTAAA TTT^AAA DraIII 871 CACATGGTG CAC[ACGT][ACGT][ACGT]^GTG EagI 74 CGGCCG C^GGCCG Eam1105I 496 GACACGAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EclXI 74 CGGCCG C^GGCCG Eco52I 74 CGGCCG C^GGCCG EcoNI 183 CCTAGACGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoT22I 562 ATGCAT ATGCA^T Hin1I 257 GGCGCC G[GA]^CG[CT]C HincII 208 GTCAAC GT[CT]^[GA]AC HindII 208 GTCAAC GT[CT]^[GA]AC KasI 257 GGCGCC G^GCGCC KpnI 362 GGTACC GGTAC^C KspI 292 CCGCGG CCGC^GG MaeI 184 CTAG C^TAG MaeII 1034 ACGT A^CGT NaeI 76 GCCGGC GCC^GGC NarI 257 GGCGCC GG^CGCC NgoMIV 76 GCCGGC G^CCGGC NsiI 562 ATGCAT ATGCA^T PaeR7I 1163 CTCGAG C^TCGAG PmaCI 1033 CACGTG CAC^GTG PmlI 1033 CACGTG CAC^GTG PshAI 413 GACCCCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC SacII 292 CCGCGG CCGC^GG ScaI 1181 AGTACT AGT^ACT SexAI 1234 ACCTGGT A^CC[AT]GGT SfoI 257 GGCGCC GGC^GCC SmlI 1163 CTCGAG C^T[CT][GA]AG SphI 1421 GCATGC GCATG^C StuI 319 AGGCCT AGG^CCT TfiI 659 GAATC G^A[AT]TC TspRI 980 CAGTG CA[GC]TG[ACGT][ACGT]^ XcmI 1655 CCACAGCTCTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoI 1163 CTCGAG C^TCGAG XmnI 1050 GAATGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 33 enzymes) # ################################### AocI 322 CCTCAGG CC^T[ACGT]AGG 905 CCTCAGG AspI 187 GACGAGGTC GAC[ACGT]^[ACGT][ACGT]GTC 1299 GACACAGTC BanII 972 GAGCCC G[GA]GC[CT]^C 1535 GGGCCC BspMI 443 ACCTGC ACCTGC(4/8) 1373 ACCTGC BsrFI 76 GCCGGC [GA]^CCGG[CT] 954 ACCGGC BsrI 854 CCAGT ACTGG(1/-1) 997 CCAGT BstAPI 555 GCATCACATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 1327 GCAGAGGGTGC BstEII 503 GGTCACC G^GT[ACGT]ACC 1746 GGTCACC BstF5I 565 CATCC GGATG(2/0) 680 CATCC BstPI 503 GGTCACC G^GT[ACGT]ACC 1746 GGTCACC BstXI 1085 CCAAGGTGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1131 CCAGATCCTTGG BstYI 911 GGATCC [GA]^GATC[CT] 1133 AGATCC Bsu36I 322 CCTCAGG CC^T[ACGT]AGG 905 CCTCAGG Cfr10I 76 GCCGGC [GA]^CCGG[CT] 954 ACCGGC DsaI 292 CCGCGG C^C[GA][CT]GG 1413 CCACGG EaeI 74 CGGCCG [CT]^GGCC[GA] 1436 CGGCCA EarI 1269 GAAGAG CTCTTC(1/4) 1275 GAAGAG Eco81I 322 CCTCAGG CC^T[ACGT]AGG 905 CCTCAGG EcoO65I 503 GGTCACC G^GT[ACGT]ACC 1746 GGTCACC FokI 565 CATCC GGATG(9/13) 680 CATCC Ksp632I 1269 GAAGAG CTCTTC(1/4) 1275 GAAGAG MflI 911 GGATCC [GA]^GATC[CT] 1133 AGATCC MseI 341 TTAA T^TAA 1127 TTAA NspI 560 ACATGC [GA]CATG^[CT] 1421 GCATGC PflFI 187 GACGAGGTC GAC[ACGT]^[ACGT][ACGT]GTC 1299 GACACAGTC PpuMI 179 GGGACCT [GA]G^G[AT]CC[CT] 345 AGGACCC SfaNI 555 GCATC GCATC(5/9) 564 GCATC SmaI 1244 CCCGGG CCC^GGG 1532 CCCGGG Tru9I 341 TTAA T^TAA 1127 TTAA Tth111I 187 GACGAGGTC GAC[ACGT]^[ACGT][ACGT]GTC 1299 GACACAGTC XhoII 911 GGATCC [GA]^GATC[CT] 1133 AGATCC XmaCI 1244 CCCGGG C^CCGGG 1532 CCCGGG XmaI 1244 CCCGGG C^CCGGG 1532 CCCGGG ######################################### # 3 OR MORE CUT ENZYMES ( 70 enzymes) # ######################################### AccII AciI AfaI AluI AlwI AvaI AvaII BanI BbvI BcnI BglI BmyI BpmI BsaJI BseRI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BssKI BstNI BstUI Cac8I CfoI Cfr13I DdeI DpnII DraII EcoO109I EcoRII EcoT14I FauI Fnu4HI HaeII HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI MaeIII MboI MboII MnlI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV PleI PstI PvuII RsaI Sau3AI Sau96I ScrFI SfcI StyI TaqI TseI Tsp45I TthHB8I