(Circular) (Six-base) MAPSORT of: pFN21AB7891.seq Check: 2660 from: 1 to: 6469 pFN21AB7891 6469 bp With 182 enzymes: SgfI * August 16, 2010 14:45 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4279 278 Size: 53 83 186 936 2743 2468 Fragments arranged by size: 2743 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3812 1937 Size: 1875 4594 Acc65I G'GTAC_C Cuts at: 2367 3790 2367 Size: 1423 5046 AclI AA'CG_TT Cuts at: 1563 4596 4969 1563 Size: 3033 373 3063 Fragments arranged by size: 3063 3033 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3134 4532 5916 6184 1632 Size: 1502 1398 1384 268 1917 Fragments arranged by size: 1917 1502 1398 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6272 AflIII A'CryG_T Cuts at: 1203 5368 6345 1203 Size: 4165 977 1327 Fragments arranged by size: 4165 1327 977 AgeI A'CCGG_T Cuts at: 5300 5300 Size: 6469 AhdI GACnn_n'nnGTC Cuts at: 2506 5198 2506 Size: 2692 3777 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6469 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2465 1184 Size: 1281 5188 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2324 2356 2324 Size: 32 6437 AlwNI CAG_nnn'CTG Cuts at: 3379 4212 5784 3379 Size: 833 1572 4064 Fragments arranged by size: 4064 1572 833 ApaI G_GGCC'C Cuts at: 3544 3544 Size: 6469 ApaLI G'TGCA_C Cuts at: 3106 4526 5682 3106 Size: 1420 1156 3893 Fragments arranged by size: 3893 1420 1156 ApoI r'AATT_y Cuts at: 1493 3778 4004 4156 6265 1493 Size: 2285 226 152 2109 1697 Fragments arranged by size: 2285 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 5023 160 Size: 872 3991 1606 Fragments arranged by size: 3991 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6469 AvaI C'yCGr_G Cuts at: 1870 1943 2099 3168 3249 3386 3537 3794 1870 Size: 73 156 1069 81 137 151 257 4545 Fragments arranged by size: 4545 1069 257 156 151 137 81 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2342 2375 2342 Size: 33 6436 BamHI G'GATC_C Cuts at: 2916 3799 4166 2916 Size: 883 367 5219 Fragments arranged by size: 5219 883 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2262 2367 2808 2962 3338 Size: 325 217 627 475 105 441 154 376 Cuts at: 3338 3790 5245 618 Size: 452 1455 1842 Fragments arranged by size: 1842 1455 627 475 452 441 376 325 217 154 105 BanII G_rGCy'C Cuts at: 1391 2981 3544 3788 1391 Size: 1590 563 244 4072 Fragments arranged by size: 4072 1590 563 244 BbeI G_GCGC'C Cuts at: 2266 2266 Size: 6469 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5498 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 4660 4694 1903 Size: 34 50 34 2639 34 3678 Fragments arranged by size: 3678 2639 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4361 5571 4361 Size: 1210 5259 BclI T'GATC_A Cuts at: 1548 1548 Size: 6469 BfrBI ATG'CAT Cuts at: 2569 2569 Size: 6469 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2078 3548 3713 Size: 107 122 71 631 605 406 1470 165 Cuts at: 3713 5080 136 Size: 1367 1525 Fragments arranged by size: 1525 1470 1367 631 605 406 165 122 107 71 BglII A'GATC_T Cuts at: 6464 6464 Size: 6469 BlpI GC'TnA_GC Cuts at: 3886 3886 Size: 6469 Bme1580I G_kGCm'C Cuts at: 1790 3110 3343 3544 4530 5686 1790 Size: 1320 233 201 986 1156 2573 Fragments arranged by size: 2573 1320 1156 986 233 201 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6469 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4200 5158 442 Size: 416 53 83 399 24 2783 958 1753 Fragments arranged by size: 2783 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6469 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2343 2343 Size: 6469 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2050 2467 2502 2842 5129 1239 Size: 811 417 35 340 2287 2579 Fragments arranged by size: 2579 2287 811 417 340 35 Bpu10I CC'TnA_GC Cuts at: 2393 2975 3579 2393 Size: 582 604 5283 Fragments arranged by size: 5283 604 582 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4594 5459 5757 4594 Size: 865 298 5306 Fragments arranged by size: 5306 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2860 5132 882 Size: 617 1361 2272 2219 Fragments arranged by size: 2272 2219 1361 617 BsaAI yAC'GTr Cuts at: 493 1204 3040 4260 493 Size: 711 1836 1220 2702 Fragments arranged by size: 2702 1836 1220 711 BsaBI GATnn'nnATC Cuts at: 6463 6463 Size: 6469 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2263 4276 4658 5293 Size: 53 83 186 936 730 2013 382 635 Cuts at: 5293 275 Size: 1451 Fragments arranged by size: 2013 1451 936 730 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4902 5300 5574 5721 6132 4902 Size: 398 274 147 411 5239 Fragments arranged by size: 5239 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2594 2624 1178 Size: 30 165 30 1191 30 5023 Fragments arranged by size: 5023 1191 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2159 2448 2459 2862 3035 3403 3583 3586 Size: 973 289 11 403 173 368 180 3 Cuts at: 3586 1186 Size: 4069 Fragments arranged by size: 4069 973 403 368 289 180 173 11 3 BseYI C'CCAG_C Cuts at: 2010 2969 3504 3543 3735 5672 6168 2010 Size: 959 535 39 192 1937 496 2311 Fragments arranged by size: 2311 1937 959 535 496 192 39 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3144 1163 Size: 573 1408 4488 Fragments arranged by size: 4488 1408 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2082 3919 4680 5708 1540 Size: 275 186 81 1837 761 1028 2301 Fragments arranged by size: 2301 1837 1028 761 275 186 81 BsiHKAI G_wGCw'C Cuts at: 3110 4530 4615 5686 3110 Size: 1420 85 1071 3893 Fragments arranged by size: 3893 1420 1071 85 BsmI GAATG_Cn' Cuts at: 3061 3980 4073 3061 Size: 919 93 5457 Fragments arranged by size: 5457 919 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2941 2941 Size: 6469 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2981 3110 3343 3544 3788 4530 4615 Size: 399 1191 129 233 201 244 742 85 Cuts at: 4615 5686 1391 Size: 1071 2174 Fragments arranged by size: 2174 1191 1071 742 399 244 233 201 129 85 BspEI T'CCGG_A Cuts at: 6132 6132 Size: 6469 BspHI T'CATG_A Cuts at: 4358 6088 4358 Size: 1730 4739 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2457 3387 3824 844 Size: 1613 930 437 3489 Fragments arranged by size: 3489 1613 930 437 BsrBI CCG'CTC Cuts at: 1394 1823 3211 4356 1394 Size: 429 1388 1145 3507 Fragments arranged by size: 3507 1388 1145 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2605 4964 5138 65 Size: 1183 1357 2359 174 1396 Fragments arranged by size: 2359 1396 1357 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2081 2959 5113 5300 1700 Size: 110 271 878 2154 187 2869 Fragments arranged by size: 2869 2154 878 271 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6469 BssHII G'CGCG_C Cuts at: 1925 2438 1925 Size: 513 5956 BssSI C'ACGA_G Cuts at: 2503 4529 5541 2503 Size: 2026 1012 3431 Fragments arranged by size: 3431 2026 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2566 3338 794 Size: 1772 772 3925 Fragments arranged by size: 3925 1772 772 BstBI TT'CG_AA Cuts at: 5273 5273 Size: 6469 BstEII G'GTnAC_C Cuts at: 1184 2508 3751 1184 Size: 1324 1243 3902 Fragments arranged by size: 3902 1324 1243 BstXI CCAn_nnnn'nTGG Cuts at: 3097 3143 3097 Size: 46 6423 BstYI r'GATC_y Cuts at: 1972 2916 3138 3799 4166 4551 4568 6020 6106 Size: 944 222 661 367 385 17 1452 86 Cuts at: 6106 6118 6464 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 944 661 385 367 346 222 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2328 2911 2328 Size: 583 5886 BtgI C'CryG_G Cuts at: 513 1065 2297 3418 513 Size: 552 1232 1121 3564 Fragments arranged by size: 3564 1232 1121 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5730 Fragments arranged by size: 5730 683 56 BtsI GCAGTG_nn' Cuts at: 2991 3985 4779 4799 2991 Size: 994 794 20 4661 Fragments arranged by size: 4661 994 794 20 ClaI AT'CG_AT Cuts at: 1554 2649 1554 Size: 1095 5374 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6434 DraI TTT'AAA Cuts at: 2347 3773 2347 Size: 1426 5043 DraIII CAC_nnn'GTG Cuts at: 2881 2881 Size: 6469 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5476 809 Size: 507 4160 1802 Fragments arranged by size: 4160 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2079 3441 3916 Size: 54 1425 50 275 18 249 1362 475 Cuts at: 3916 4805 6434 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 1362 889 475 275 249 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2079 3916 1537 Size: 275 267 1837 4090 Fragments arranged by size: 4090 1837 275 267 EarI CTCTTCn'nnn_ Cuts at: 1353 3269 3275 4399 1353 Size: 1916 6 1124 3423 Fragments arranged by size: 3423 1916 1124 6 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2100 2270 5056 5430 5576 387 Size: 1064 138 511 170 2786 374 146 1280 Fragments arranged by size: 2786 1280 1064 511 374 170 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2050 2467 2502 2842 3134 4532 5129 Size: 393 418 417 35 340 292 1398 597 Cuts at: 5129 5916 6184 1239 Size: 787 268 1524 Fragments arranged by size: 1524 1398 787 597 418 417 393 340 292 268 35 EcoNI CCTnn'n_nnAGG Cuts at: 2192 2192 Size: 6469 EcoO109I rG'GnC_Cy Cuts at: 2185 2351 3715 2185 Size: 166 1364 4939 Fragments arranged by size: 4939 1364 166 EcoRI G'AATT_C Cuts at: 3778 4156 3778 Size: 378 6091 FspI TGC'GCA Cuts at: 4975 4975 Size: 6469 HaeII r_GCGC'y Cuts at: 1678 2073 2172 2266 2490 5616 1678 Size: 395 99 94 224 3126 2531 Fragments arranged by size: 3126 2531 395 224 99 94 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2156 2186 2188 2218 2465 Size: 32 135 32 584 30 2 30 247 Cuts at: 2465 2492 2497 2524 2594 2626 5110 5142 5184 Size: 27 5 27 70 32 2484 32 42 Cuts at: 5184 5216 1373 Size: 32 2626 Fragments arranged by size: 2626 2484 584 247 135 70 42 32 32 32 32 32 30 30 27 27 5 2 HincII GTy'rAC Cuts at: 669 1657 1938 2215 3813 4059 669 Size: 988 281 277 1598 246 3079 Fragments arranged by size: 3079 1598 988 281 277 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6469 HpaI GTT'AAC Cuts at: 4059 4059 Size: 6469 KasI G'GCGC_C Cuts at: 2262 2262 Size: 6469 KpnI G_GTAC'C Cuts at: 2371 3794 2371 Size: 1423 5046 MfeI C'AATT_G Cuts at: 4068 4068 Size: 6469 MluI A'CGCG_T Cuts at: 6345 6345 Size: 6469 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2202 2436 2707 5583 5767 1305 Size: 897 234 271 2876 184 2007 Fragments arranged by size: 2876 2007 897 271 234 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4647 Fragments arranged by size: 4647 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 4427 4786 4945 518 Size: 648 3261 359 159 2042 Fragments arranged by size: 3261 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2049 2182 2299 2575 3295 3473 3547 3885 Size: 443 133 117 276 720 178 74 338 Cuts at: 3885 4562 5710 5955 1606 Size: 677 1148 245 2120 Fragments arranged by size: 2120 1148 720 677 443 338 276 245 178 133 117 74 NaeI GCC'GGC Cuts at: 2083 2083 Size: 6469 NarI GG'CG_CC Cuts at: 2263 2263 Size: 6469 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5917 NdeI CA'TA_TG Cuts at: 387 387 Size: 6469 NgoMIV G'CCGG_C Cuts at: 2081 2081 Size: 6469 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6469 NotI GC'GGCC_GC Cuts at: 3916 3916 Size: 6469 NsiI A_TGCA'T Cuts at: 2571 2571 Size: 6469 NspI r_CATG'y Cuts at: 1132 1229 2569 3430 3827 5372 1132 Size: 97 1340 861 397 1545 2229 Fragments arranged by size: 2229 1545 1340 861 397 97 PasI CC'CwG_GG Cuts at: 1348 2363 1348 Size: 1015 5454 PciI A'CATG_T Cuts at: 5368 5368 Size: 6469 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6469 PfoI T'CCnGG_A Cuts at: 2127 2277 2385 2428 2716 3324 2127 Size: 150 108 43 288 608 5272 Fragments arranged by size: 5272 608 288 150 108 43 PmeI GTTT'AAAC Cuts at: 3773 3773 Size: 6469 PmlI CAC'GTG Cuts at: 3040 3040 Size: 6469 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4538 4570 6051 6083 4538 Size: 32 1481 32 4924 Fragments arranged by size: 4924 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2185 2351 2185 Size: 166 6303 PshAI GACnn'nnGTC Cuts at: 1613 2422 1613 Size: 809 5660 PsiI TTA'TAA Cuts at: 4039 4039 Size: 6469 PspOMI G'GGCC_C Cuts at: 3540 3540 Size: 6469 PstI C_TGCA'G Cuts at: 830 2454 2986 3457 3589 3821 830 Size: 1624 532 471 132 232 3478 Fragments arranged by size: 3478 1624 532 471 232 132 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6469 PvuII CAG'CTG Cuts at: 3295 3473 3547 3295 Size: 178 74 6217 Fragments arranged by size: 6217 178 74 SacII CC_GC'GG Cuts at: 2300 2300 Size: 6469 SalI G'TCGA_C Cuts at: 1936 3811 1936 Size: 1875 4594 SbfI CC_TGCA'GG Cuts at: 3821 3821 Size: 6469 ScaI AGT'ACT Cuts at: 3188 4717 6429 3188 Size: 1529 1712 3228 Fragments arranged by size: 3228 1712 1529 SexAI A'CCwGG_T Cuts at: 3239 3239 Size: 6469 SfcI C'TryA_G Cuts at: 826 1045 2450 2593 2982 3453 3585 3728 3817 Size: 219 1405 143 389 471 132 143 89 Cuts at: 3817 4952 5633 5824 826 Size: 1135 681 191 1471 Fragments arranged by size: 1471 1405 1135 681 471 389 219 191 143 143 132 89 SfoI GGC'GCC Cuts at: 2264 2264 Size: 6469 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6469 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6469 SmaI CCC'GGG Cuts at: 1872 3251 3539 3796 1872 Size: 1379 288 257 4545 Fragments arranged by size: 4545 1379 288 257 SmlI C'TyrA_G Cuts at: 820 1017 1943 3168 4573 5474 5736 820 Size: 197 926 1225 1405 901 262 1553 Fragments arranged by size: 1553 1405 1225 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4260 493 Size: 3767 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6469 SphI G_CATG'C Cuts at: 1132 3430 3827 1132 Size: 2298 397 3774 Fragments arranged by size: 3774 2298 397 SspI AAT'ATT Cuts at: 5 52 4393 5 Size: 47 4341 2081 Fragments arranged by size: 4341 2081 47 StuI AGG'CCT Cuts at: 1517 2326 1517 Size: 809 5660 StyI C'CwwG_G Cuts at: 513 1065 3090 3142 3646 3908 513 Size: 552 2025 52 504 262 3074 Fragments arranged by size: 3074 2025 552 504 262 52 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2645 2697 3503 4496 4513 4666 4851 Size: 1255 1065 52 806 993 17 153 185 Cuts at: 4851 5296 325 Size: 445 1498 Fragments arranged by size: 1498 1255 1065 993 806 445 185 153 52 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2781 3186 4715 Size: 274 80 33 51 1443 804 405 1529 Cuts at: 4715 6427 96 Size: 1712 138 Fragments arranged by size: 1712 1529 1443 804 405 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6429 6461 6429 Size: 32 6437 Tth111I GACn'n_nGTC Cuts at: 1316 2195 3307 1316 Size: 879 1112 4478 Fragments arranged by size: 4478 1112 879 XbaI T'CTAG_A Cuts at: 3805 3805 Size: 6469 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3667 3667 Size: 6469 XhoI C'TCGA_G Cuts at: 1943 3168 1943 Size: 1225 5244 XmaI C'CCGG_G Cuts at: 1870 3249 3537 3794 1870 Size: 1379 288 257 4545 Fragments arranged by size: 4545 1379 288 257 XmnI GAAnn'nnTTC Cuts at: 3059 4598 3059 Size: 1539 4930 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4277 276 Size: 53 83 186 936 2743 2468 Fragments arranged by size: 2743 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacII SalI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AscI AvrII BbvCI BsiWI BstZ17I EcoICRI EcoRV FalI FseI FspAI NruI PacI PspXI PsrI RsrII SacI SanDI SapI SfiI SrfI SwaI