(Linear) (Six-base) MAPSORT of: KIBB7891.seq Check: 238 from: 1 to: 1764 KIBB7891.orf With 182 enzymes: SgfI * July 15, 2010 18:31 .. Acc65I G'GTAC_C Cuts at: 0 362 1764 Size: 362 1402 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1129 1764 Size: 1129 635 AhdI GACnn_n'nnGTC Cuts at: 0 501 1764 Size: 501 1263 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 460 1764 Size: 460 1304 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 319 351 1764 Size: 319 32 1413 Fragments arranged by size: 1413 319 32 AlwNI CAG_nnn'CTG Cuts at: 0 1374 1764 Size: 1374 390 ApaI G_GGCC'C Cuts at: 0 1539 1764 Size: 1539 225 ApaLI G'TGCA_C Cuts at: 0 1101 1764 Size: 1101 663 AvaI C'yCGr_G Cuts at: 0 94 1163 1244 1381 1532 1764 Size: 94 1069 81 137 151 232 Fragments arranged by size: 1069 232 151 137 94 81 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 0 337 370 1764 Size: 337 33 1394 Fragments arranged by size: 1394 337 33 BamHI G'GATC_C Cuts at: 0 911 1764 Size: 911 853 BanI G'GyrC_C Cuts at: 0 257 362 803 957 1333 1764 Size: 257 105 441 154 376 431 Fragments arranged by size: 441 431 376 257 154 105 BanII G_rGCy'C Cuts at: 0 976 1539 1764 Size: 976 563 225 Fragments arranged by size: 976 563 225 BbeI G_GCGC'C Cuts at: 0 261 1764 Size: 261 1503 BfrBI ATG'CAT Cuts at: 0 564 1764 Size: 564 1200 BglI GCCn_nnn'nGGC Cuts at: 0 73 1543 1708 1764 Size: 73 1470 165 56 Fragments arranged by size: 1470 165 73 56 Bme1580I G_kGCm'C Cuts at: 0 1105 1338 1539 1764 Size: 1105 233 201 225 Fragments arranged by size: 1105 233 225 201 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 338 1764 Size: 338 1426 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 45 462 497 837 1764 Size: 45 417 35 340 927 Fragments arranged by size: 927 417 340 45 35 Bpu10I CC'TnA_GC Cuts at: 0 388 970 1574 1764 Size: 388 582 604 190 Fragments arranged by size: 604 582 388 190 BsaI GGTCTCn'nnnn_ Cuts at: 0 855 1764 Size: 855 909 BsaAI yAC'GTr Cuts at: 0 1035 1764 Size: 1035 729 BsaHI Gr'CG_yC Cuts at: 0 258 1764 Size: 258 1506 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 589 619 1764 Size: 589 30 1145 Fragments arranged by size: 1145 589 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 154 443 454 857 1030 1398 1578 1581 Size: 154 289 11 403 173 368 180 3 Cuts at: 1581 1764 Size: 183 Fragments arranged by size: 403 368 289 183 180 173 154 11 3 BseYI C'CCAG_C Cuts at: 0 5 964 1499 1538 1730 1764 Size: 5 959 535 39 192 34 Fragments arranged by size: 959 535 192 39 34 5 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1139 1764 Size: 1139 625 BsiEI CG_ry'CG Cuts at: 0 77 1764 Size: 77 1687 BsiHKAI G_wGCw'C Cuts at: 0 1105 1764 Size: 1105 659 BsmI GAATG_Cn' Cuts at: 0 1056 1764 Size: 1056 708 BsmBI CGTCTCn'nnnn_ Cuts at: 0 936 1764 Size: 936 828 Bsp1286I G_dGCh'C Cuts at: 0 976 1105 1338 1539 1764 Size: 976 129 233 201 225 Fragments arranged by size: 976 233 225 201 129 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 452 1382 1764 Size: 452 930 382 Fragments arranged by size: 930 452 382 BsrBI CCG'CTC Cuts at: 0 1206 1764 Size: 1206 558 BsrDI GCAATG_nn' Cuts at: 0 600 1764 Size: 600 1164 BsrFI r'CCGG_y Cuts at: 0 76 954 1764 Size: 76 878 810 Fragments arranged by size: 878 810 76 BssHII G'CGCG_C Cuts at: 0 433 1764 Size: 433 1331 BssSI C'ACGA_G Cuts at: 0 498 1764 Size: 498 1266 BstAPI GCAn_nnn'nTGC Cuts at: 0 561 1333 1764 Size: 561 772 431 Fragments arranged by size: 772 561 431 BstEII G'GTnAC_C Cuts at: 0 503 1746 1764 Size: 503 1243 18 Fragments arranged by size: 1243 503 18 BstXI CCAn_nnnn'nTGG Cuts at: 0 1092 1138 1764 Size: 1092 46 626 Fragments arranged by size: 1092 626 46 BstYI r'GATC_y Cuts at: 0 911 1133 1764 Size: 911 222 631 Fragments arranged by size: 911 631 222 Bsu36I CC'TnA_GG Cuts at: 0 323 906 1764 Size: 323 583 858 Fragments arranged by size: 858 583 323 BtgI C'CryG_G Cuts at: 0 292 1413 1764 Size: 292 1121 351 Fragments arranged by size: 1121 351 292 BtsI GCAGTG_nn' Cuts at: 0 986 1764 Size: 986 778 ClaI AT'CG_AT Cuts at: 0 644 1764 Size: 644 1120 DraI TTT'AAA Cuts at: 0 342 1764 Size: 342 1422 DraIII CAC_nnn'GTG Cuts at: 0 876 1764 Size: 876 888 EaeI y'GGCC_r Cuts at: 0 74 1436 1764 Size: 74 1362 328 Fragments arranged by size: 1362 328 74 EagI C'GGCC_G Cuts at: 0 74 1764 Size: 74 1690 EarI CTCTTCn'nnn_ Cuts at: 0 1264 1270 1764 Size: 1264 6 494 Fragments arranged by size: 1264 494 6 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 95 265 1764 Size: 95 170 1499 Fragments arranged by size: 1499 170 95 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 45 462 497 837 1129 1764 Size: 45 417 35 340 292 635 Fragments arranged by size: 635 417 340 292 45 35 EcoNI CCTnn'n_nnAGG Cuts at: 0 187 1764 Size: 187 1577 EcoO109I rG'GnC_Cy Cuts at: 0 180 346 1710 1764 Size: 180 166 1364 54 Fragments arranged by size: 1364 180 166 54 HaeII r_GCGC'y Cuts at: 0 68 167 261 485 1764 Size: 68 99 94 224 1279 Fragments arranged by size: 1279 224 99 94 68 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 151 181 183 213 460 487 492 519 Size: 151 30 2 30 247 27 5 27 Cuts at: 519 589 621 1764 Size: 70 32 1143 Fragments arranged by size: 1143 247 151 70 32 30 30 27 27 5 2 HincII GTy'rAC Cuts at: 0 210 1764 Size: 210 1554 KasI G'GCGC_C Cuts at: 0 257 1764 Size: 257 1507 KpnI G_GTAC'C Cuts at: 0 366 1764 Size: 366 1398 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 197 431 702 1764 Size: 197 234 271 1062 Fragments arranged by size: 1062 271 234 197 MspA1I CmG'CkG Cuts at: 0 44 177 294 570 1290 1468 1542 1764 Size: 44 133 117 276 720 178 74 222 Fragments arranged by size: 720 276 222 178 133 117 74 44 NaeI GCC'GGC Cuts at: 0 78 1764 Size: 78 1686 NarI GG'CG_CC Cuts at: 0 258 1764 Size: 258 1506 NgoMIV G'CCGG_C Cuts at: 0 76 1764 Size: 76 1688 NsiI A_TGCA'T Cuts at: 0 566 1764 Size: 566 1198 NspI r_CATG'y Cuts at: 0 564 1425 1764 Size: 564 861 339 Fragments arranged by size: 861 564 339 PasI CC'CwG_GG Cuts at: 0 358 1764 Size: 358 1406 PfoI T'CCnGG_A Cuts at: 0 122 272 380 423 711 1319 1764 Size: 122 150 108 43 288 608 445 Fragments arranged by size: 608 445 288 150 122 108 43 PmlI CAC'GTG Cuts at: 0 1035 1764 Size: 1035 729 PpuMI rG'GwC_Cy Cuts at: 0 180 346 1764 Size: 180 166 1418 Fragments arranged by size: 1418 180 166 PshAI GACnn'nnGTC Cuts at: 0 417 1764 Size: 417 1347 PspOMI G'GGCC_C Cuts at: 0 1535 1764 Size: 1535 229 PstI C_TGCA'G Cuts at: 0 449 981 1452 1584 1764 Size: 449 532 471 132 180 Fragments arranged by size: 532 471 449 180 132 PvuII CAG'CTG Cuts at: 0 1290 1468 1542 1764 Size: 1290 178 74 222 Fragments arranged by size: 1290 222 178 74 SacII CC_GC'GG Cuts at: 0 295 1764 Size: 295 1469 ScaI AGT'ACT Cuts at: 0 1183 1764 Size: 1183 581 SexAI A'CCwGG_T Cuts at: 0 1234 1764 Size: 1234 530 SfcI C'TryA_G Cuts at: 0 445 588 977 1448 1580 1723 1764 Size: 445 143 389 471 132 143 41 Fragments arranged by size: 471 445 389 143 143 132 41 SfoI GGC'GCC Cuts at: 0 259 1764 Size: 259 1505 SmaI CCC'GGG Cuts at: 0 1246 1534 1764 Size: 1246 288 230 Fragments arranged by size: 1246 288 230 SmlI C'TyrA_G Cuts at: 0 1163 1764 Size: 1163 601 SphI G_CATG'C Cuts at: 0 1425 1764 Size: 1425 339 StuI AGG'CCT Cuts at: 0 321 1764 Size: 321 1443 StyI C'CwwG_G Cuts at: 0 1085 1137 1641 1764 Size: 1085 52 504 123 Fragments arranged by size: 1085 504 123 52 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 640 692 1498 1764 Size: 640 52 806 266 Fragments arranged by size: 806 640 266 52 TatI w'GTAC_w Cuts at: 0 776 1181 1764 Size: 776 405 583 Fragments arranged by size: 776 583 405 Tth111I GACn'n_nGTC Cuts at: 0 190 1302 1764 Size: 190 1112 462 Fragments arranged by size: 1112 462 190 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 1662 1764 Size: 1662 102 XhoI C'TCGA_G Cuts at: 0 1163 1764 Size: 1163 601 XmaI C'CCGG_G Cuts at: 0 1244 1532 1764 Size: 1244 288 232 Fragments arranged by size: 1244 288 232 XmnI GAAnn'nnTTC Cuts at: 0 1054 1764 Size: 1054 710 Enzymes that do cut: Acc65I AcuI AhdI AlfI AloI AlwNI ApaI ApaLI AvaI BaeI BamHI BanI BanII BbeI BfrBI BglI Bme1580I BplI BpmI Bpu10I BsaI BsaAI BsaHI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspMI BsrBI BsrDI BsrFI BssHII BssSI BstAPI BstEII BstXI BstYI Bsu36I BtgI BtsI ClaI DraI DraIII EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I HaeII Hin4I HincII KasI KpnI MmeI MspA1I NaeI NarI NgoMIV NsiI NspI PasI PfoI PmlI PpuMI PshAI PspOMI PstI PvuII SacII ScaI SexAI SfcI SfoI SmaI SmlI SphI StuI StyI TaqII TatI Tth111I XcmI XhoI XmaI XmnI Enzymes that do not cut: AarI AatII AccI AclI AfeI AflII AflIII AgeI AleI ApoI AscI AseI AsiSI AvrII BbsI BbvCI BcgI BciVI BclI BglII BlpI BmgBI BmrI BmtI BpuEI BsaBI BsaWI BsiWI BspEI BspHI BsrGI BstBI BstZ17I BtgZI CspCI DrdI EcoICRI EcoRI EcoRV FalI FseI FspI FspAI HindIII HpaI MfeI MluI MscI MslI NcoI NdeI NheI NotI NruI PacI PciI PflMI PmeI PpiI PsiI PspXI PsrI PvuI RsrII SacI SalI SanDI SapI SbfI SfiI SgfI SgrAI SnaBI SpeI SrfI SspI SwaI TstI XbaI ZraI