################################## # NO CUT ENZYMES ( 101 enzymes) # ################################## AatII Acc65I AccIII AclI AflIII AgeI AhdI Alw44I AlwNI Aor51HI ApaLI AscI AseI AsnI Asp718I AspEI AspI AviII AvrII BbrPI BcgI BlnI BsaI BseAI BsiEI BsiWI BsmBI BsmI BspDI BspEI BspHI BspLU11I BsrFI Bst1107I BstAPI BstBI BstEII BstPI BstZ17I Cfr10I ClaI CpoI DraIII DrdI EagI Eam1105I EclXI Eco47III Eco52I EcoNI EcoO65I EcoRV FseI FspI HgaI HpaI KpnI KspI MfeI MluI MroI MslI MspA1I MunI NaeI NdeI NgoMIV NheI NotI NruI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI PvuII RcaI RsrII SacII SalI SbfI ScaI SfuI SgrAI SpeI Sse8387I SwaI Tth111I Van91I XcmI XhoI ################################### # ONE CUT ENZYMES ( 53 enzymes) # ################################### AccI 2114 GTCTAC GT^[AC][GT]AC AflII 1534 CTTAAG C^TTAAG AocI 867 CCTGAGG CC^T[ACGT]AGG AspHI 216 GAGCTC G[AT]GC[AT]^C AvaI 111 CCCGGG C^[CT]CG[GA]G AvaII 108 GGTCC G^G[AT]CC BalI 446 TGGCCA TGG^CCA BamHI 1710 GGATCC G^GATCC BbvCI 607 GCTGAGG CCTCAGC(-5/-2) BciVI 1878 GTATCC GTATCC(6/5) BclI 333 TGATCA T^GATCA BfrI 1534 CTTAAG C^TTAAG BglI 188 GCCCTCGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BglII 729 AGATCT A^GATCT BlpI 79 GCTGAGC GC^T[ACGT]AGC BpmI 140 CTGGAG CTGGAG(16/14) Bpu1102I 79 GCTGAGC GC^T[ACGT]AGC BsaAI 886 TACGTA [CT]AC^GT[GA] BsaBI 1522 GATTGGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsgI 2198 CTGCAC GTGCAG(16/14) BsiHKAI 216 GAGCTC G[AT]GC[AT]^C BsoBI 111 CCCGGG C^[CT]CG[GA]G Bsp1407I 480 TGTACA T^GTACA BspMI 156 GCAGGT ACCTGC(4/8) BsrBI 858 CCGCTC CCGCTC(-3/-3) BsrGI 480 TGTACA T^GTACA BsrI 793 CCAGT ACTGG(1/-1) BssSI 191 CTCGTG CACGAG(-5/-1) BstXI 2119 CCAGAAAAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG Bsu36I 867 CCTGAGG CC^T[ACGT]AGG CelII 79 GCTGAGC GC^T[ACGT]AGC DsaI 1567 CCATGG C^C[GA][CT]GG Eco81I 867 CCTGAGG CC^T[ACGT]AGG FbaI 333 TGATCA T^GATCA HincII 1719 GTCAAC GT[CT]^[GA]AC HindII 1719 GTCAAC GT[CT]^[GA]AC MamI 1522 GATTGGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MluNI 446 TGGCCA TGG^CCA MscI 446 TGGCCA TGG^CCA NcoI 1567 CCATGG C^CATGG SacI 216 GAGCTC GAGCT^C SexAI 2099 ACCAGGT A^CC[AT]GGT SfiI 187 GGCCCTCGTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SmaI 111 CCCGGG CCC^GGG SmlI 1534 CTTAAG C^T[CT][GA]AG SnaBI 886 TACGTA TAC^GTA SphI 1053 GCATGC GCATG^C SspBI 480 TGTACA T^GTACA StuI 1947 AGGCCT AGG^CCT Tsp45I 1340 GTGAC ^GT[GC]AC XbaI 2212 TCTAGA T^CTAGA XmaCI 111 CCCGGG C^CCGGG XmaI 111 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 30 enzymes) # ################################### AcyI 61 GGCGCC G[GA]^CG[CT]C 90 GGCGCC ApaI 186 GGGCCC GGGCC^C 452 GGGCCC Asp700I 1435 GAAAAGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 2242 GAAATAATTC BanI 61 GGCGCC G^G[CT][GA]CC 90 GGCGCC BbeI 61 GGCGCC GGCGC^C 90 GGCGCC BbsI 1235 GAAGAC GAAGAC(2/6) 345 GTCTTC BpuAI 1235 GAAGAC GAAGAC(2/6) 345 GTCTTC BsaHI 61 GGCGCC G[GA]^CG[CT]C 90 GGCGCC BsaWI 105 TCCGGT [AT]^CCGG[AT] 176 ACCGGA BsmAI 722 GAGAC GTCTC(1/5) 1153 GAGAC BsmFI 165 GGGAC GGGAC(10/14) 109 GTCCC BsrDI 1750 CATTGC GCAATG(2/0) 1425 GCAATG BssHII 33 GCGCGC G^CGCGC 152 GCGCGC EaeI 195 TGGCCG [CT]^GGCC[GA] 446 TGGCCA EcoRI 1699 GAATTC G^AATTC 2221 GAATTC EcoT14I 1335 CCAAGG C^C[AT][AT]GG 1567 CCATGG EcoT22I 1051 ATGCAT ATGCA^T 1476 ATGCAT HaeII 61 GGCGCC [GA]GCGC^[CT] 90 GGCGCC Hin1I 61 GGCGCC G[GA]^CG[CT]C 90 GGCGCC KasI 61 GGCGCC G^GCGCC 90 GGCGCC NarI 61 GGCGCC GG^CGCC 90 GGCGCC NsiI 1051 ATGCAT ATGCA^T 1476 ATGCAT NspI 1053 GCATGC [GA]CATG^[CT] 2110 GCATGT SapI 127 GAAGAGC GCTCTTC(1/4) 1956 GCTCTTC SfoI 61 GGCGCC GGC^GCC 90 GGCGCC SspI 1405 AATATT AAT^ATT 1966 AATATT StyI 1335 CCAAGG C^C[AT][AT]GG 1567 CCATGG TaqI 1221 TCGA T^CGA 2237 TCGA TthHB8I 1221 TCGA T^CGA 2237 TCGA XmnI 1435 GAAAAGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 2242 GAAATAATTC ######################################### # 3 OR MORE CUT ENZYMES ( 74 enzymes) # ######################################### AccII AciI AcsI AfaI AluI AlwI ApoI BanII BbvI BcnI BfaI BmyI BsaJI BseRI BsiYI BslI Bsp1286I BssKI BstF5I BstNI BstUI BstYI Cac8I CfoI Cfr13I DdeI DpnII DraI DraII EarI EcoO109I EcoRII FauI Fnu4HI FokI HaeIII HapII HhaI HinP1I HindIII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV PleI RsaI Sau3AI Sau96I ScrFI SfaNI SfcI TfiI Tru9I TseI Tsp509I TspRI XhoII