(Circular) (Six-base) MAPSORT of: pFN21AB6282.seq Check: 9641 from: 1 to: 7048 pFN21AB6282 7048 bp With 182 enzymes: SgfI * March 28, 2012 12:38 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2152 2152 Size: 7048 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4858 278 Size: 53 83 186 936 3322 2468 Fragments arranged by size: 3322 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4120 4391 1937 Size: 2183 271 4594 Fragments arranged by size: 4594 2183 271 Acc65I G'GTAC_C Cuts at: 4369 4369 Size: 7048 AclI AA'CG_TT Cuts at: 1563 5175 5548 1563 Size: 3612 373 3063 Fragments arranged by size: 3612 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3294 5111 6495 6763 1632 Size: 1662 1817 1384 268 1917 Fragments arranged by size: 1917 1817 1662 1384 268 AflII C'TTAA_G Cuts at: 820 1017 3539 820 Size: 197 2522 4329 Fragments arranged by size: 4329 2522 197 AflIII A'CryG_T Cuts at: 1203 5947 6924 1203 Size: 4744 977 1327 Fragments arranged by size: 4744 1327 977 AgeI A'CCGG_T Cuts at: 5879 5879 Size: 7048 AhdI GACnn_n'nnGTC Cuts at: 5777 5777 Size: 7048 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 7048 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 7048 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2097 2129 2097 Size: 32 7016 AlwNI CAG_nnn'CTG Cuts at: 4791 6363 4791 Size: 1572 5476 ApaI G_GGCC'C Cuts at: 2195 2461 2195 Size: 266 6782 ApaLI G'TGCA_C Cuts at: 5105 6261 5105 Size: 1156 5892 ApoI r'AATT_y Cuts at: 1493 2771 2803 2966 2985 2998 3422 3593 3704 Size: 1278 32 163 19 13 424 171 111 Cuts at: 3704 4141 4226 4357 4583 4735 6844 1493 Size: 437 85 131 226 152 2109 1697 Fragments arranged by size: 2109 1697 1278 437 424 226 171 163 152 131 111 85 32 19 13 AseI AT'TA_AT Cuts at: 160 1032 5602 160 Size: 872 4570 1606 Fragments arranged by size: 4570 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7048 AvaI C'yCGr_G Cuts at: 1870 1943 2116 4373 1870 Size: 73 173 2257 4545 Fragments arranged by size: 4545 2257 173 73 BamHI G'GATC_C Cuts at: 3715 4378 4745 3715 Size: 663 367 6018 Fragments arranged by size: 6018 663 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2066 2095 4369 5824 618 Size: 325 217 627 279 29 2274 1455 1842 Fragments arranged by size: 2274 1842 1455 627 325 279 217 29 BanII G_rGCy'C Cuts at: 1391 2195 2225 2461 4367 1391 Size: 804 30 236 1906 4072 Fragments arranged by size: 4072 1906 804 236 30 BbeI G_GCGC'C Cuts at: 2070 2099 2070 Size: 29 7019 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2343 3247 928 Size: 971 444 904 4729 Fragments arranged by size: 4729 971 904 444 BbvCI CC'TCA_GC Cuts at: 2613 2613 Size: 7048 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5239 5273 1903 Size: 34 3302 34 3678 Fragments arranged by size: 3678 3302 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3894 4940 6150 3894 Size: 1046 1210 4792 Fragments arranged by size: 4792 1210 1046 BclI T'GATC_A Cuts at: 1548 2338 1548 Size: 790 6258 BfrBI ATG'CAT Cuts at: 3058 3483 3058 Size: 425 6623 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2199 5659 136 Size: 107 122 71 631 605 527 3460 1525 Fragments arranged by size: 3460 1525 631 605 527 122 107 71 BglII A'GATC_T Cuts at: 2734 7043 2734 Size: 4309 2739 BlpI GC'TnA_GC Cuts at: 2085 4465 2085 Size: 2380 4668 Bme1580I G_kGCm'C Cuts at: 1790 2195 2461 5109 6265 1790 Size: 405 266 2648 1156 2573 Fragments arranged by size: 2648 2573 1156 405 266 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 7048 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4779 5737 442 Size: 416 53 83 399 24 3362 958 1753 Fragments arranged by size: 3362 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7048 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2158 2158 Size: 7048 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2166 5708 1239 Size: 927 3542 2579 Fragments arranged by size: 3542 2579 927 Bpu10I CC'TnA_GC Cuts at: 2613 2613 Size: 7048 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 5173 6038 6336 5173 Size: 865 298 5885 Fragments arranged by size: 5885 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5711 882 Size: 617 4212 2219 Fragments arranged by size: 4212 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2893 4839 493 Size: 711 1689 1946 2702 Fragments arranged by size: 2702 1946 1689 711 BsaBI GATnn'nnATC Cuts at: 3531 7042 3531 Size: 3511 3537 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2067 2096 4855 5237 Size: 53 83 186 936 534 29 2759 382 Cuts at: 5237 5872 275 Size: 635 1451 Fragments arranged by size: 2759 1451 936 635 534 382 186 83 53 29 BsaWI w'CCGG_w Cuts at: 2110 2181 5481 5879 6153 6300 6711 2110 Size: 71 3300 398 274 147 411 2447 Fragments arranged by size: 3300 2447 411 398 274 147 71 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6823 Fragments arranged by size: 6823 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2087 2163 2311 2488 1186 Size: 901 76 148 177 5746 Fragments arranged by size: 5746 901 177 148 76 BseYI C'CCAG_C Cuts at: 6251 6747 6251 Size: 496 6552 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 4188 1163 Size: 573 2452 4023 Fragments arranged by size: 4023 2452 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4498 5259 6287 1540 Size: 275 186 2497 761 1028 2301 Fragments arranged by size: 2497 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2225 5109 5194 6265 2225 Size: 2884 85 1071 3008 Fragments arranged by size: 3008 2884 1071 85 BsmI GAATG_Cn' Cuts at: 4559 4652 4559 Size: 93 6955 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2195 2225 2461 4367 5109 5194 6265 Size: 399 405 30 236 1906 742 85 1071 Cuts at: 6265 1391 Size: 2174 Fragments arranged by size: 2174 1906 1071 742 405 399 236 85 30 BspEI T'CCGG_A Cuts at: 6711 6711 Size: 7048 BspHI T'CATG_A Cuts at: 4937 6667 4937 Size: 1730 5318 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2152 4403 844 Size: 1308 2251 3489 Fragments arranged by size: 3489 2251 1308 BsrBI CCG'CTC Cuts at: 1394 1823 2865 4935 1394 Size: 429 1042 2070 3507 Fragments arranged by size: 3507 2070 1042 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3437 3754 5543 5717 65 Size: 1183 2189 317 1789 174 1396 Fragments arranged by size: 2189 1789 1396 1183 317 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5692 5879 1700 Size: 110 3882 187 2869 Fragments arranged by size: 3882 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 2485 96 Size: 2389 4659 BssHII G'CGCG_C Cuts at: 1925 2038 2157 1925 Size: 113 119 6816 Fragments arranged by size: 6816 119 113 BssSI C'ACGA_G Cuts at: 2196 5108 6120 2196 Size: 2912 1012 3124 Fragments arranged by size: 3124 2912 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 7048 BstBI TT'CG_AA Cuts at: 5852 5852 Size: 7048 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7048 BstXI CCAn_nnnn'nTGG Cuts at: 4131 4131 Size: 7048 BstYI r'GATC_y Cuts at: 1972 2403 2734 2753 3715 4378 4745 5130 5147 Size: 431 331 19 962 663 367 385 17 Cuts at: 5147 6599 6685 6697 7043 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 962 663 431 385 367 346 331 86 19 17 12 Bsu36I CC'TnA_GG Cuts at: 2873 2873 Size: 7048 BtgI C'CryG_G Cuts at: 513 1065 2004 3572 513 Size: 552 939 1568 3989 Fragments arranged by size: 3989 1568 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2076 487 Size: 683 56 850 5459 Fragments arranged by size: 5459 850 683 56 BtsI GCAGTG_nn' Cuts at: 2755 3613 4564 5358 5378 2755 Size: 858 951 794 20 4425 Fragments arranged by size: 4425 951 858 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7048 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7013 DraI TTT'AAA Cuts at: 3072 3498 3561 4352 3072 Size: 426 63 791 5768 Fragments arranged by size: 5768 791 426 63 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6055 809 Size: 507 4739 1802 Fragments arranged by size: 4739 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2200 2451 4495 Size: 54 1425 50 275 18 370 251 2044 Cuts at: 4495 5384 7013 8 Size: 889 1629 43 Fragments arranged by size: 2044 1629 1425 889 370 275 251 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4495 1537 Size: 275 2683 4090 Fragments arranged by size: 4090 2683 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2127 2295 2510 3207 3968 4978 1353 Size: 774 168 215 697 761 1010 3423 Fragments arranged by size: 3423 1010 774 761 697 215 168 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2784 5635 6009 6155 387 Size: 1064 138 1195 2851 374 146 1280 Fragments arranged by size: 2851 1280 1195 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2223 2223 Size: 7048 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2166 3294 5111 5708 6495 6763 1239 Size: 393 534 1128 1817 597 787 268 1524 Fragments arranged by size: 1817 1524 1128 787 597 534 393 268 EcoO109I rG'GnC_Cy Cuts at: 2191 2192 2457 2191 Size: 1 265 6782 Fragments arranged by size: 6782 265 1 EcoRI G'AATT_C Cuts at: 3704 4226 4357 4735 3704 Size: 522 131 378 6017 Fragments arranged by size: 6017 522 378 131 FspI TGC'GCA Cuts at: 5554 5554 Size: 7048 HaeII r_GCGC'y Cuts at: 1678 2070 2099 6195 1678 Size: 392 29 4096 2531 Fragments arranged by size: 4096 2531 392 29 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2249 2281 2927 2959 3146 Size: 32 135 32 677 32 646 32 187 Cuts at: 3146 3178 3206 3208 3238 3240 3707 3739 5689 Size: 32 28 2 30 2 467 32 1950 Cuts at: 5689 5721 5763 5795 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1950 677 646 467 187 135 42 32 32 32 32 32 32 32 32 30 28 2 2 HincII GTy'rAC Cuts at: 669 1657 1938 3726 4392 4638 669 Size: 988 281 1788 666 246 3079 Fragments arranged by size: 3079 1788 988 666 281 246 HindIII A'AGCT_T Cuts at: 748 3465 3491 4275 748 Size: 2717 26 784 3521 Fragments arranged by size: 3521 2717 784 26 HpaI GTT'AAC Cuts at: 4638 4638 Size: 7048 KasI G'GCGC_C Cuts at: 2066 2095 2066 Size: 29 7019 KpnI G_GTAC'C Cuts at: 4373 4373 Size: 7048 MfeI C'AATT_G Cuts at: 4647 4647 Size: 7048 MluI A'CGCG_T Cuts at: 6924 6924 Size: 7048 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3525 6162 6346 1305 Size: 2220 2637 184 2007 Fragments arranged by size: 2637 2220 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2453 10 Size: 54 1425 343 621 4605 Fragments arranged by size: 4605 1425 621 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 5006 5365 5524 518 Size: 648 3840 359 159 2042 Fragments arranged by size: 3840 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 4464 5141 6289 6534 1606 Size: 2858 677 1148 245 2120 Fragments arranged by size: 2858 2120 1148 677 245 NarI GG'CG_CC Cuts at: 2067 2096 2067 Size: 29 7019 NcoI C'CATG_G Cuts at: 513 1065 2004 3572 513 Size: 552 939 1568 3989 Fragments arranged by size: 3989 1568 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 7048 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7048 NotI GC'GGCC_GC Cuts at: 4495 4495 Size: 7048 NsiI A_TGCA'T Cuts at: 3060 3485 3060 Size: 425 6623 NspI r_CATG'y Cuts at: 1132 1229 3062 4119 4406 5951 1132 Size: 97 1833 1057 287 1545 2229 Fragments arranged by size: 2229 1833 1545 1057 287 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 7048 PciI A'CATG_T Cuts at: 5947 5947 Size: 7048 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 7048 PmeI GTTT'AAAC Cuts at: 4352 4352 Size: 7048 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5117 5149 6630 6662 5117 Size: 32 1481 32 5503 Fragments arranged by size: 5503 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7048 PsiI TTA'TAA Cuts at: 2816 4618 2816 Size: 1802 5246 PspOMI G'GGCC_C Cuts at: 2191 2457 2191 Size: 266 6782 PstI C_TGCA'G Cuts at: 830 4400 830 Size: 3570 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7048 SacI G_AGCT'C Cuts at: 2225 2225 Size: 7048 SalI G'TCGA_C Cuts at: 1936 4390 1936 Size: 2454 4594 SapI GCTCTTCn'nnn_ Cuts at: 2127 3968 2127 Size: 1841 5207 SbfI CC_TGCA'GG Cuts at: 4400 4400 Size: 7048 ScaI AGT'ACT Cuts at: 5296 7008 5296 Size: 1712 5336 SexAI A'CCwGG_T Cuts at: 4104 4104 Size: 7048 SfcI C'TryA_G Cuts at: 826 1045 2849 3585 3897 4396 5531 6212 6403 Size: 219 1804 736 312 499 1135 681 191 Cuts at: 6403 826 Size: 1471 Fragments arranged by size: 1804 1471 1135 736 681 499 312 219 191 SfiI GGCCn_nnn'nGGCC Cuts at: 2199 2199 Size: 7048 SfoI GGC'GCC Cuts at: 2068 2097 2068 Size: 29 7019 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7048 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7048 SmaI CCC'GGG Cuts at: 1872 2118 4375 1872 Size: 246 2257 4545 Fragments arranged by size: 4545 2257 246 SmlI C'TyrA_G Cuts at: 820 1017 1943 3539 5152 6053 6315 820 Size: 197 926 1596 1613 901 262 1553 Fragments arranged by size: 1613 1596 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 2893 4839 493 Size: 2400 1946 2702 Fragments arranged by size: 2702 2400 1946 SpeI A'CTAG_T Cuts at: 152 152 Size: 7048 SphI G_CATG'C Cuts at: 1132 3062 4406 1132 Size: 1930 1344 3774 Fragments arranged by size: 3774 1930 1344 SspI AAT'ATT Cuts at: 5 52 3412 3973 4972 5 Size: 47 3360 561 999 2081 Fragments arranged by size: 3360 2081 999 561 47 StuI AGG'CCT Cuts at: 1517 3954 1517 Size: 2437 4611 StyI C'CwwG_G Cuts at: 513 1065 2004 3340 3572 4487 513 Size: 552 939 1336 232 915 3074 Fragments arranged by size: 3074 1336 939 915 552 232 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 5075 5092 5245 5430 5875 325 Size: 1255 3495 17 153 185 445 1498 Fragments arranged by size: 3495 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2485 5294 7006 Size: 274 80 33 51 1443 508 2809 1712 Cuts at: 7006 96 Size: 138 Fragments arranged by size: 2809 1712 1443 508 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 7008 7040 7008 Size: 32 7016 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7048 XbaI T'CTAG_A Cuts at: 4217 4384 4217 Size: 167 6881 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7048 XmaI C'CCGG_G Cuts at: 1870 2116 4373 1870 Size: 246 2257 4545 Fragments arranged by size: 4545 2257 246 XmnI GAAnn'nnTTC Cuts at: 3444 4251 5177 3444 Size: 807 926 5315 Fragments arranged by size: 5315 926 807 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4856 276 Size: 53 83 186 936 3322 2468 Fragments arranged by size: 3322 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PspOMI PstI PvuI SacI SalI SapI SbfI ScaI SexAI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AscI AvrII BaeI BsiWI BsmBI BstZ17I DraIII EcoNI EcoRV FalI FseI FspAI NaeI NgoMIV NruI PacI PfoI PmlI PpuMI PspXI PsrI PvuII RsrII SacII SanDI SrfI SwaI XcmI