(Linear) (Six-base) MAPSORT of: KIBB6282.seq Check: 8610 from: 1 to: 2343 KIBB6282.orf With 182 enzymes: SgfI * March 22, 2012 15:34 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 147 2343 Size: 147 2196 AccI GT'mk_AC Cuts at: 0 2115 2343 Size: 2115 228 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1289 2343 Size: 1289 1054 AflII C'TTAA_G Cuts at: 0 1534 2343 Size: 1534 809 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 92 124 2343 Size: 92 32 2219 Fragments arranged by size: 2219 92 32 ApaI G_GGCC'C Cuts at: 0 190 456 2343 Size: 190 266 1887 Fragments arranged by size: 1887 266 190 ApoI r'AATT_y Cuts at: 0 766 798 961 980 993 1417 1588 1699 Size: 766 32 163 19 13 424 171 111 Cuts at: 1699 2136 2221 2343 Size: 437 85 122 Fragments arranged by size: 766 437 424 171 163 122 111 85 32 19 13 AvaI C'yCGr_G Cuts at: 0 111 2343 Size: 111 2232 BamHI G'GATC_C Cuts at: 0 1710 2343 Size: 1710 633 BanI G'GyrC_C Cuts at: 0 61 90 2343 Size: 61 29 2253 Fragments arranged by size: 2253 61 29 BanII G_rGCy'C Cuts at: 0 190 220 456 2343 Size: 190 30 236 1887 Fragments arranged by size: 1887 236 190 30 BbeI G_GCGC'C Cuts at: 0 65 94 2343 Size: 65 29 2249 Fragments arranged by size: 2249 65 29 BbsI GAAGACnn'nnnn_ Cuts at: 0 338 1242 2343 Size: 338 904 1101 Fragments arranged by size: 1101 904 338 BbvCI CC'TCA_GC Cuts at: 0 608 2343 Size: 608 1735 BciVI GTATCCnnnnn_n' Cuts at: 0 1889 2343 Size: 1889 454 BclI T'GATC_A Cuts at: 0 333 2343 Size: 333 2010 BfrBI ATG'CAT Cuts at: 0 1053 1478 2343 Size: 1053 425 865 Fragments arranged by size: 1053 865 425 BglI GCCn_nnn'nGGC Cuts at: 0 194 2343 Size: 194 2149 BglII A'GATC_T Cuts at: 0 729 2343 Size: 729 1614 BlpI GC'TnA_GC Cuts at: 0 80 2343 Size: 80 2263 Bme1580I G_kGCm'C Cuts at: 0 190 456 2343 Size: 190 266 1887 Fragments arranged by size: 1887 266 190 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 153 2343 Size: 153 2190 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 161 2343 Size: 161 2182 Bpu10I CC'TnA_GC Cuts at: 0 608 2343 Size: 608 1735 BsaAI yAC'GTr Cuts at: 0 888 2343 Size: 888 1455 BsaBI GATnn'nnATC Cuts at: 0 1526 2343 Size: 1526 817 BsaHI Gr'CG_yC Cuts at: 0 62 91 2343 Size: 62 29 2252 Fragments arranged by size: 2252 62 29 BsaWI w'CCGG_w Cuts at: 0 105 176 2343 Size: 105 71 2167 Fragments arranged by size: 2167 105 71 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 82 158 306 483 2343 Size: 82 76 148 177 1860 Fragments arranged by size: 1860 177 148 82 76 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 2183 2343 Size: 2183 160 BsiHKAI G_wGCw'C Cuts at: 0 220 2343 Size: 220 2123 Bsp1286I G_dGCh'C Cuts at: 0 190 220 456 2343 Size: 190 30 236 1887 Fragments arranged by size: 1887 236 190 30 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 147 2343 Size: 147 2196 BsrBI CCG'CTC Cuts at: 0 860 2343 Size: 860 1483 BsrDI GCAATG_nn' Cuts at: 0 1432 1749 2343 Size: 1432 317 594 Fragments arranged by size: 1432 594 317 BsrGI T'GTAC_A Cuts at: 0 480 2343 Size: 480 1863 BssHII G'CGCG_C Cuts at: 0 33 152 2343 Size: 33 119 2191 Fragments arranged by size: 2191 119 33 BssSI C'ACGA_G Cuts at: 0 191 2343 Size: 191 2152 BstXI CCAn_nnnn'nTGG Cuts at: 0 2126 2343 Size: 2126 217 BstYI r'GATC_y Cuts at: 0 398 729 748 1710 2343 Size: 398 331 19 962 633 Fragments arranged by size: 962 633 398 331 19 Bsu36I CC'TnA_GG Cuts at: 0 868 2343 Size: 868 1475 BtgI C'CryG_G Cuts at: 0 1567 2343 Size: 1567 776 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 71 2343 Size: 71 2272 BtsI GCAGTG_nn' Cuts at: 0 750 1608 2343 Size: 750 858 735 Fragments arranged by size: 858 750 735 DraI TTT'AAA Cuts at: 0 1067 1493 1556 2343 Size: 1067 426 63 787 Fragments arranged by size: 1067 787 426 63 EaeI y'GGCC_r Cuts at: 0 195 446 2343 Size: 195 251 1897 Fragments arranged by size: 1897 251 195 EarI CTCTTCn'nnn_ Cuts at: 0 122 290 505 1202 1963 2343 Size: 122 168 215 697 761 380 Fragments arranged by size: 761 697 380 215 168 122 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 779 2343 Size: 779 1564 EcoICRI GAG'CTC Cuts at: 0 218 2343 Size: 218 2125 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 161 1289 2343 Size: 161 1128 1054 Fragments arranged by size: 1128 1054 161 EcoO109I rG'GnC_Cy Cuts at: 0 186 187 452 2343 Size: 186 1 265 1891 Fragments arranged by size: 1891 265 186 1 EcoRI G'AATT_C Cuts at: 0 1699 2221 2343 Size: 1699 522 122 Fragments arranged by size: 1699 522 122 HaeII r_GCGC'y Cuts at: 0 65 94 2343 Size: 65 29 2249 Fragments arranged by size: 2249 65 29 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 244 276 922 954 1141 1173 1201 1203 Size: 244 32 646 32 187 32 28 2 Cuts at: 1203 1233 1235 1702 1734 2343 Size: 30 2 467 32 609 Fragments arranged by size: 646 609 467 244 187 32 32 32 32 30 28 2 2 HincII GTy'rAC Cuts at: 0 1721 2343 Size: 1721 622 HindIII A'AGCT_T Cuts at: 0 1460 1486 2270 2343 Size: 1460 26 784 73 Fragments arranged by size: 1460 784 73 26 KasI G'GCGC_C Cuts at: 0 61 90 2343 Size: 61 29 2253 Fragments arranged by size: 2253 61 29 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 1520 2343 Size: 1520 823 MscI TGG'CCA Cuts at: 0 448 2343 Size: 448 1895 NarI GG'CG_CC Cuts at: 0 62 91 2343 Size: 62 29 2252 Fragments arranged by size: 2252 62 29 NcoI C'CATG_G Cuts at: 0 1567 2343 Size: 1567 776 NsiI A_TGCA'T Cuts at: 0 1055 1480 2343 Size: 1055 425 863 Fragments arranged by size: 1055 863 425 NspI r_CATG'y Cuts at: 0 1057 2114 2343 Size: 1057 1057 229 Fragments arranged by size: 1057 1057 229 PsiI TTA'TAA Cuts at: 0 811 2343 Size: 811 1532 PspOMI G'GGCC_C Cuts at: 0 186 452 2343 Size: 186 266 1891 Fragments arranged by size: 1891 266 186 SacI G_AGCT'C Cuts at: 0 220 2343 Size: 220 2123 SapI GCTCTTCn'nnn_ Cuts at: 0 122 1963 2343 Size: 122 1841 380 Fragments arranged by size: 1841 380 122 SexAI A'CCwGG_T Cuts at: 0 2099 2343 Size: 2099 244 SfcI C'TryA_G Cuts at: 0 844 1580 1892 2343 Size: 844 736 312 451 Fragments arranged by size: 844 736 451 312 SfiI GGCCn_nnn'nGGCC Cuts at: 0 194 2343 Size: 194 2149 SfoI GGC'GCC Cuts at: 0 63 92 2343 Size: 63 29 2251 Fragments arranged by size: 2251 63 29 SmaI CCC'GGG Cuts at: 0 113 2343 Size: 113 2230 SmlI C'TyrA_G Cuts at: 0 1534 2343 Size: 1534 809 SnaBI TAC'GTA Cuts at: 0 888 2343 Size: 888 1455 SphI G_CATG'C Cuts at: 0 1057 2343 Size: 1057 1286 SspI AAT'ATT Cuts at: 0 1407 1968 2343 Size: 1407 561 375 Fragments arranged by size: 1407 561 375 StuI AGG'CCT Cuts at: 0 1949 2343 Size: 1949 394 StyI C'CwwG_G Cuts at: 0 1335 1567 2343 Size: 1335 232 776 Fragments arranged by size: 1335 776 232 TatI w'GTAC_w Cuts at: 0 480 2343 Size: 480 1863 XbaI T'CTAG_A Cuts at: 0 2212 2343 Size: 2212 131 XmaI C'CCGG_G Cuts at: 0 111 2343 Size: 111 2232 XmnI GAAnn'nnTTC Cuts at: 0 1439 2246 2343 Size: 1439 807 97 Fragments arranged by size: 1439 807 97 Enzymes that do cut: AarI AccI AcuI AflII AloI ApaI ApoI AvaI BamHI BanI BanII BbeI BbsI BbvCI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BplI BpmI Bpu10I BsaAI BsaBI BsaHI BsaWI BseRI BsgI BsiHKAI Bsp1286I BspMI BsrBI BsrDI BsrGI BssHII BssSI BstXI BstYI Bsu36I BtgI BtgZI BtsI DraI EaeI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI HaeII Hin4I HincII HindIII KasI MmeI MscI NarI NcoI NsiI NspI PsiI PspOMI SacI SapI SexAI SfcI SfiI SfoI SmaI SmlI SnaBI SphI SspI StuI StyI TatI XbaI XmaI XmnI Enzymes that do not cut: AatII Acc65I AclI AfeI AflIII AgeI AhdI AleI AlfI AlwNI ApaLI AscI AseI AsiSI AvrII BaeI BcgI BmgBI BmrI BmtI BpuEI BsaI BsaXI BseYI BsiEI BsiWI BsmI BsmBI BspEI BspHI BsrFI BstAPI BstBI BstEII BstZ17I ClaI CspCI DraIII DrdI EagI EcoNI EcoRV FalI FseI FspI FspAI HpaI KpnI MfeI MluI MslI MspA1I NaeI NdeI NgoMIV NheI NotI NruI PacI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PspXI PsrI PstI PvuI PvuII RsrII SacII SalI SanDI SbfI ScaI SgfI SgrAI SpeI SrfI SwaI TaqII TstI Tth111I XcmI XhoI ZraI