Restriction map for
pFN21AB5834
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# NO CUT ENZYMES ( 105 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AflII AflIII AgeI Alw44I
ApaLI ApoI AscI AseI AsnI
Asp700I Asp718I AvrII BalI BbrPI
BcgI BciVI BclI BfrI BglII
BlnI BsaBI BsaWI BseAI BsiWI
BsmBI BsmI BspDI BspEI BspHI
BspLU11I BssHII BssSI Bst1107I BstBI
BstEII BstPI BstXI BstZ17I ClaI
CpoI DraI DraIII DrdI EcoNI
EcoO65I EcoRI EcoRV FbaI HincII
HindII HpaI KpnI KspI MamI
MfeI MluI MluNI MroI MscI
MseI MunI NcoI NdeI NheI
NotI NruI NspV PacI PinAI
PmaCI PmeI PmlI PpuMI PshBI
Psp1406I PstI PvuI RcaI RsrII
SacII SalI SbfI ScaI SfcI
SfiI SfuI SgrAI SmaI SnaBI
SpeI Sse8387I SspI StuI SwaI
TfiI Tru9I XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 39 enzymes) #
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AspI 634 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbsI 69 GAAGAC GAAGAC(2/6)
BbvCI 525 GCTGAGG CCTCAGC(-5/-2)
BfaI 1397 CTAG C^TAG
BpuAI 69 GAAGAC GAAGAC(2/6)
BsaAI 520 TACGTG [CT]AC^GT[GA]
BsaI 235 GAGACC GGTCTC(1/5)
BspMI 293 ACCTGC ACCTGC(4/8)
BsrBI 1362 GAGCGG CCGCTC(-3/-3)
BsrDI 912 CATTGC GCAATG(2/0)
BstAPI 1035 GCAGCAGTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
EarI 1359 GAAGAG CTCTTC(1/4)
EcoT22I 1154 ATGCAT ATGCA^T
FauI 1364 GCGGG CCCGC(4/6)
FseI 657 GGCCGGCC GGCCGG^CC
HindIII 400 AAGCTT A^AGCTT
Ksp632I 1359 GAAGAG CTCTTC(1/4)
MaeI 1397 CTAG C^TAG
MslI 1157 CATGGTGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NaeI 658 GCCGGC GCC^GGC
NgoMIV 658 GCCGGC G^CCGGC
NsiI 1154 ATGCAT ATGCA^T
NspI 1026 GCATGC [GA]CATG^[CT]
PaeR7I 118 CTCGAG C^TCGAG
PflFI 634 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 56 CCAAGAGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshAI 1416 GACTGGGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuII 366 CAGCTG CAG^CTG
SapI 1359 GAAGAGC GCTCTTC(1/4)
SexAI 1296 ACCTGGT A^CC[AT]GGT
SmlI 118 CTCGAG C^T[CT][GA]AG
SphI 1026 GCATGC GCATG^C
Tsp45I 226 GTCAC ^GT[GC]AC
Tsp509I 461 AATT ^AATT
Tth111I 634 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 56 CCAAGAGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 1396 TCTAGA T^CTAGA
XcmI 589 CCACTCCTGTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 118 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 40 enzymes) #
###################################
AcyI 253 GGCGCC G[GA]^CG[CT]C
378 GGCGCC
AhdI 307 GACGCGCCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
958 GACAGCACGTC
ApaI 566 GGGCCC GGGCC^C
1278 GGGCCC
AspEI 307 GACGCGCCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
958 GACAGCACGTC
AvaII 53 GGACC G^G[AT]CC
1353 GGTCC
AviII 908 TGCGCA TGC^GCA
1029 TGCGCA
BamHI 454 GGATCC G^GATCC
1074 GGATCC
BbeI 253 GGCGCC GGCGC^C
378 GGCGCC
BglI 506 GCCTGCATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
671 GCCCCTCAGGC
BlpI 280 GCTCAGC GC^T[ACGT]AGC
889 GCTGAGC
Bpu1102I 280 GCTCAGC GC^T[ACGT]AGC
889 GCTGAGC
BsaHI 253 GGCGCC G[GA]^CG[CT]C
378 GGCGCC
BseRI 862 CTCCTC GAGGAG(10/8)
1183 GAGGAG
BsiEI 302 CGGCCG CG[GA][CT]^CG
660 CGGCCG
BsmAI 235 GAGAC GTCTC(1/5)
736 GAGAC
Bsp1407I 434 TGTACA T^GTACA
1343 TGTACA
BsrFI 658 GCCGGC [GA]^CCGG[CT]
749 ACCGGC
BsrGI 434 TGTACA T^GTACA
1343 TGTACA
BstF5I 744 CATCC GGATG(2/0)
1152 GGATG
BstYI 454 GGATCC [GA]^GATC[CT]
1074 GGATCC
CelII 280 GCTCAGC GC^T[ACGT]AGC
889 GCTGAGC
Cfr10I 658 GCCGGC [GA]^CCGG[CT]
749 ACCGGC
EagI 302 CGGCCG C^GGCCG
660 CGGCCG
Eam1105I 307 GACGCGCCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
958 GACAGCACGTC
EclXI 302 CGGCCG C^GGCCG
660 CGGCCG
Eco52I 302 CGGCCG C^GGCCG
660 CGGCCG
EcoT14I 953 CCTTGG C^C[AT][AT]GG
1148 CCAAGG
FokI 744 CATCC GGATG(9/13)
1152 GGATG
FspI 908 TGCGCA TGC^GCA
1029 TGCGCA
Hin1I 253 GGCGCC G[GA]^CG[CT]C
378 GGCGCC
KasI 253 GGCGCC G^GCGCC
378 GGCGCC
MaeII 521 ACGT A^CGT
964 ACGT
MaeIII 226 GTCAC ^GT[ACGT]AC
469 GTAAC
MflI 454 GGATCC [GA]^GATC[CT]
1074 GGATCC
MspA1I 366 CAGCTG C[AC]G^C[GT]G
666 CCGCTG
NarI 253 GGCGCC GG^CGCC
378 GGCGCC
SfoI 253 GGCGCC GGC^GCC
378 GGCGCC
SspBI 434 TGTACA T^GTACA
1343 TGTACA
StyI 953 CCTTGG C^C[AT][AT]GG
1148 CCAAGG
XhoII 454 GGATCC [GA]^GATC[CT]
1074 GGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 74 enzymes) #
#########################################
AccII AciI AfaI AluI AlwI
AlwNI AocI Aor51HI AspHI AvaI
BanI BanII BbvI BcnI BmyI
BpmI BsaJI BsgI BsiHKAI BsiYI
BslI BsmFI BsoBI Bsp1286I BsrI
BssKI BstNI BstUI Bsu36I Cac8I
CfoI Cfr13I DdeI DpnII DraII
DsaI EaeI Eco47III Eco81I EcoO109I
EcoRII Fnu4HI HaeII HaeIII HapII
HgaI HhaI HinP1I HinfI HpaII
HphI ItaI MboI MboII MnlI
MspI MvaI MvnI MwoI NciI
NdeII NlaIII NlaIV PleI RsaI
SacI Sau3AI Sau96I ScrFI SfaNI
TaqI TseI TspRI TthHB8I