Restriction map for
pFN21AB5834
(Circular) (Six-base) MAPSORT of: pFN21AB5834.seq Check: 9965 from: 1 to: 6148
pFN21AB5834 6148 bp
With 182 enzymes: SgfI *
January 31, 2011 17:47 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2307 2307
Size: 6148
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3958 278
Size: 53 83 186 936 2422 2468
Fragments arranged by size:
2468 2422 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3491 1937
Size: 1554 4594
Acc65I G'GTAC_C
Cuts at: 3469 3469
Size: 6148
AclI AA'CG_TT
Cuts at: 1563 4275 4648 1563
Size: 2712 373 3063
Fragments arranged by size:
3063 2712 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3071 4211 5595 5863 1632
Size: 1439 1140 1384 268 1917
Fragments arranged by size:
1917 1439 1384 1140 268
AfeI AGC'GCT
Cuts at: 2051 2291 2504 2051
Size: 240 213 5695
Fragments arranged by size:
5695 240 213
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5951
AflIII A'CryG_T
Cuts at: 1203 5047 6024 1203
Size: 3844 977 1327
Fragments arranged by size:
3844 1327 977
AgeI A'CCGG_T
Cuts at: 4979 4979
Size: 6148
AhdI GACnn_n'nnGTC
Cuts at: 2317 2968 4877 2317
Size: 651 1909 3588
Fragments arranged by size:
3588 1909 651
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6148
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3184 1184
Size: 2000 4148
AlwNI CAG_nnn'CTG
Cuts at: 2344 2587 2687 3049 3891 5463 2344
Size: 243 100 362 842 1572 3029
Fragments arranged by size:
3029 1572 842 362 243 100
ApaI G_GGCC'C
Cuts at: 2575 3287 2575
Size: 712 5436
ApaLI G'TGCA_C
Cuts at: 4205 5361 4205
Size: 1156 4992
ApoI r'AATT_y
Cuts at: 1493 3457 3683 3835 5944 1493
Size: 1964 226 152 2109 1697
Fragments arranged by size:
2109 1964 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4702 160
Size: 872 3670 1606
Fragments arranged by size:
3670 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6148
AvaI C'yCGr_G
Cuts at: 1870 1943 2123 3144 3177 3473 1870
Size: 73 180 1021 33 296 4545
Fragments arranged by size:
4545 1021 296 180 73 33
BamHI G'GATC_C
Cuts at: 2459 3079 3478 3845 2459
Size: 620 399 367 4762
Fragments arranged by size:
4762 620 399 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2258 2383 3168 3469 4924
Size: 325 217 627 471 125 785 301 1455
Cuts at: 4924 618
Size: 1842
Fragments arranged by size:
1842 1455 785 627 471 325 301 217
125
BanII G_rGCy'C
Cuts at: 1391 2153 2174 2256 2575 2868 2901 3153 3204
Size: 762 21 82 319 293 33 252 51
Cuts at: 3204 3287 3399 3443 3467 1391
Size: 83 112 44 24 4072
Fragments arranged by size:
4072 762 319 293 252 112 83 82
51 44 33 24 21
BbeI G_GCGC'C
Cuts at: 2262 2387 2262
Size: 125 6023
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2081 928
Size: 971 182 4995
Fragments arranged by size:
4995 971 182
BbvCI CC'TCA_GC
Cuts at: 2531 2531
Size: 6148
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4339 4373 1903
Size: 34 2402 34 3678
Fragments arranged by size:
3678 2402 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4040 5250 4040
Size: 1210 4938
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6148
BfrBI ATG'CAT
Cuts at: 3161 3161
Size: 6148
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2517 2682 4759
Size: 107 122 71 631 605 845 165 2077
Cuts at: 4759 136
Size: 1525
Fragments arranged by size:
2077 1525 845 631 605 165 122 107
71
BglII A'GATC_T
Cuts at: 6143 6143
Size: 6148
BlpI GC'TnA_GC
Cuts at: 2286 2895 3565 2286
Size: 609 670 4869
Fragments arranged by size:
4869 670 609
Bme1580I G_kGCm'C
Cuts at: 1790 2575 2790 3287 4209 5365 1790
Size: 785 215 497 922 1156 2573
Fragments arranged by size:
2573 1156 922 785 497 215
BmgBI CAC'GTC
Cuts at: 1318 2970 1318
Size: 1652 4496
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3107 3432 3879
Size: 416 53 83 399 24 1690 325 447
Cuts at: 3879 4837 442
Size: 958 1753
Fragments arranged by size:
1753 1690 958 447 416 399 325 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6148
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2438 2447 2609 4808 1239
Size: 1199 9 162 2199 2579
Fragments arranged by size:
2579 2199 1199 162 9
Bpu10I CC'TnA_GC
Cuts at: 2250 2403 2531 2976 2250
Size: 153 128 445 5422
Fragments arranged by size:
5422 445 153 128
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4273 5138 5436 4273
Size: 865 298 4985
Fragments arranged by size:
4985 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2234 4811 882
Size: 617 735 2577 2219
Fragments arranged by size:
2577 2219 735 617
BsaAI yAC'GTr
Cuts at: 493 1204 2527 3939 493
Size: 711 1323 1412 2702
Fragments arranged by size:
2702 1412 1323 711
BsaBI GATnn'nnATC
Cuts at: 6142 6142
Size: 6148
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2259 2384 3955 4337
Size: 53 83 186 936 726 125 1571 382
Cuts at: 4337 4972 275
Size: 635 1451
Fragments arranged by size:
1571 1451 936 726 635 382 186 125
83 53
BsaWI w'CCGG_w
Cuts at: 4581 4979 5253 5400 5811 4581
Size: 398 274 147 411 4918
Fragments arranged by size:
4918 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2976 3006 1178
Size: 30 165 30 1573 30 4320
Fragments arranged by size:
4320 1573 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2858 3203 1186
Size: 1672 345 4131
Fragments arranged by size:
4131 1672 345
BseYI C'CCAG_C
Cuts at: 2881 3286 3384 5351 5847 2881
Size: 405 98 1967 496 3182
Fragments arranged by size:
3182 1967 496 405 98
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2346 2357 2501 1163
Size: 573 610 11 144 4810
Fragments arranged by size:
4810 610 573 144 11
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2310 2668 3598 4359 5387 1540
Size: 275 186 309 358 930 761 1028 2301
Fragments arranged by size:
2301 1028 930 761 358 309 275 186
BsiHKAI G_wGCw'C
Cuts at: 2091 2153 2174 2868 3204 3254 4209 4294 5365
Size: 62 21 694 336 50 955 85 1071
Cuts at: 5365 2091
Size: 2874
Fragments arranged by size:
2874 1071 955 694 336 85 62 50
21
BsmI GAATG_Cn'
Cuts at: 3659 3752 3659
Size: 93 6055
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2091 2153 2174 2256 2575 2790 2868
Size: 399 301 62 21 82 319 215 78
Cuts at: 2868 2901 3153 3204 3254 3287 3399 3443 3467
Size: 33 252 51 50 33 112 44 24
Cuts at: 3467 4209 4294 5365 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 319 301 252 215
112 85 82 78 62 51 50 44
33 33 24 21
BspEI T'CCGG_A
Cuts at: 5811 5811
Size: 6148
BspHI T'CATG_A
Cuts at: 4037 5767 4037
Size: 1730 4418
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2307 3503 844
Size: 1463 1196 3489
Fragments arranged by size:
3489 1463 1196
BsrBI CCG'CTC
Cuts at: 1394 1823 3369 4035 1394
Size: 429 1546 666 3507
Fragments arranged by size:
3507 1546 666 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2916 4643 4817 65
Size: 1183 1668 1727 174 1396
Fragments arranged by size:
1727 1668 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2663 2754 4792 4979 1700
Size: 110 853 91 2038 187 2869
Fragments arranged by size:
2869 2038 853 187 110 91
BsrGI T'GTAC_A
Cuts at: 96 2439 3348 96
Size: 2343 909 2896
Fragments arranged by size:
2896 2343 909
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6148
BssSI C'ACGA_G
Cuts at: 4208 5220 4208
Size: 1012 5136
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3046 794
Size: 2252 3896
BstBI TT'CG_AA
Cuts at: 4952 4952
Size: 6148
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6148
BstYI r'GATC_y
Cuts at: 1972 2459 3079 3478 3845 4230 4247 5699 5785
Size: 487 620 399 367 385 17 1452 86
Cuts at: 5785 5797 6143 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 620 487 399 385 367 346
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2680 3099 3376 2680
Size: 419 277 5452
Fragments arranged by size:
5452 419 277
BtgI C'CryG_G
Cuts at: 513 1065 2004 2107 2235 2565 513
Size: 552 939 103 128 330 4096
Fragments arranged by size:
4096 939 552 330 128 103
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5409
Fragments arranged by size:
5409 683 56
BtsI GCAGTG_nn'
Cuts at: 2022 2262 2280 3325 3664 4458 4478 2022
Size: 240 18 1045 339 794 20 3692
Fragments arranged by size:
3692 1045 794 339 240 20 18
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6148
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6113
DraI TTT'AAA
Cuts at: 3452 3452
Size: 6148
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5155 809
Size: 507 3839 1802
Fragments arranged by size:
3839 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2097 2161 2307
Size: 54 1425 50 275 18 267 64 146
Cuts at: 2307 2632 2665 3595 4484 6113 8
Size: 325 33 930 889 1629 43
Fragments arranged by size:
1629 1425 930 889 325 275 267 146
64 54 50 43 33 18
EagI C'GGCC_G
Cuts at: 1537 1812 2307 2665 3595 1537
Size: 275 495 358 930 4090
Fragments arranged by size:
4090 930 495 358 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3359 4078 1353
Size: 2006 719 3423
Fragments arranged by size:
3423 2006 719
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2261 4735 5109 5255 387
Size: 1064 138 672 2474 374 146 1280
Fragments arranged by size:
2474 1280 1064 672 374 146 138
EcoICRI GAG'CTC
Cuts at: 2151 2172 2866 3202 2151
Size: 21 694 336 5097
Fragments arranged by size:
5097 694 336 21
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2438 2447 2609 3071 4211 4808 5595
Size: 393 806 9 162 462 1140 597 787
Cuts at: 5595 5863 1239
Size: 268 1524
Fragments arranged by size:
1524 1140 806 787 597 462 393 268
162 9
EcoO109I rG'GnC_Cy
Cuts at: 2571 2684 3103 3127 3283 3372 2571
Size: 113 419 24 156 89 5347
Fragments arranged by size:
5347 419 156 113 89 24
EcoRI G'AATT_C
Cuts at: 3457 3835 3457
Size: 378 5770
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2048 2048
Size: 6148
FseI GG_CCGG'CC
Cuts at: 2667 2667
Size: 6148
FspI TGC'GCA
Cuts at: 2915 3036 4654 2915
Size: 121 1618 4409
Fragments arranged by size:
4409 1618 121
HaeII r_GCGC'y
Cuts at: 1678 2053 2262 2293 2387 2506 5295 1678
Size: 375 209 31 94 119 2789 2531
Fragments arranged by size:
2789 2531 375 209 119 94 31
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2303 2335 2954 2986 4789
Size: 32 135 32 731 32 619 32 1803
Cuts at: 4789 4821 4863 4895 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1803 731 619 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3492 3738 669
Size: 988 281 1554 246 3079
Fragments arranged by size:
3079 1554 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2405 748
Size: 1657 4491
HpaI GTT'AAC
Cuts at: 3738 3738
Size: 6148
KasI G'GCGC_C
Cuts at: 2258 2383 2258
Size: 125 6023
KpnI G_GTAC'C
Cuts at: 3473 3473
Size: 6148
MfeI C'AATT_G
Cuts at: 3747 3747
Size: 6148
MluI A'CGCG_T
Cuts at: 6024 6024
Size: 6148
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5262 5446 1305
Size: 3957 184 2007
Fragments arranged by size:
3957 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4326
Fragments arranged by size:
4326 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3166 4106 4465 4624 518
Size: 648 2000 940 359 159 2042
Fragments arranged by size:
2042 2000 940 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2373 2673 3564 4241 5389 5634 1606
Size: 767 300 891 677 1148 245 2120
Fragments arranged by size:
2120 1148 891 767 677 300 245
NaeI GCC'GGC
Cuts at: 2665 2665
Size: 6148
NarI GG'CG_CC
Cuts at: 2259 2384 2259
Size: 125 6023
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4657
Fragments arranged by size:
4657 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6148
NgoMIV G'CCGG_C
Cuts at: 2663 2663
Size: 6148
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6148
NotI GC'GGCC_GC
Cuts at: 3595 3595
Size: 6148
NsiI A_TGCA'T
Cuts at: 3163 3163
Size: 6148
NspI r_CATG'y
Cuts at: 1132 1229 3035 3506 5051 1132
Size: 97 1806 471 1545 2229
Fragments arranged by size:
2229 1806 1545 471 97
PasI CC'CwG_GG
Cuts at: 1348 2767 3124 3280 1348
Size: 1419 357 156 4216
Fragments arranged by size:
4216 1419 357 156
PciI A'CATG_T
Cuts at: 5047 5047
Size: 6148
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2067 1108
Size: 959 5189
PmeI GTTT'AAAC
Cuts at: 3452 3452
Size: 6148
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4217 4249 5730 5762 4217
Size: 32 1481 32 4603
Fragments arranged by size:
4603 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 3425 1613
Size: 1812 4336
PsiI TTA'TAA
Cuts at: 3718 3718
Size: 6148
PspOMI G'GGCC_C
Cuts at: 2571 3283 2571
Size: 712 5436
PspXI vC'TCGA_Gb
Cuts at: 2123 2123
Size: 6148
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2423 2455 2423
Size: 32 6116
PstI C_TGCA'G
Cuts at: 830 3500 830
Size: 2670 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6148
PvuII CAG'CTG
Cuts at: 2373 2373
Size: 6148
SacI G_AGCT'C
Cuts at: 2153 2174 2868 3204 2153
Size: 21 694 336 5097
Fragments arranged by size:
5097 694 336 21
SalI G'TCGA_C
Cuts at: 1936 3490 1936
Size: 1554 4594
SapI GCTCTTCn'nnn_
Cuts at: 3359 3359
Size: 6148
SbfI CC_TGCA'GG
Cuts at: 3500 3500
Size: 6148
ScaI AGT'ACT
Cuts at: 4396 6108 4396
Size: 1712 4436
SexAI A'CCwGG_T
Cuts at: 3301 3301
Size: 6148
SfcI C'TryA_G
Cuts at: 826 1045 3496 4631 5312 5503 826
Size: 219 2451 1135 681 191 1471
Fragments arranged by size:
2451 1471 1135 681 219 191
SfoI GGC'GCC
Cuts at: 2260 2385 2260
Size: 125 6023
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6148
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6148
SmaI CCC'GGG
Cuts at: 1872 3475 1872
Size: 1603 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2123 4252 5153 5415 820
Size: 197 926 180 2129 901 262 1553
Fragments arranged by size:
2129 1553 926 901 262 197 180
SnaBI TAC'GTA
Cuts at: 493 3939 493
Size: 3446 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6148
SphI G_CATG'C
Cuts at: 1132 3035 3506 1132
Size: 1903 471 3774
Fragments arranged by size:
3774 1903 471
SspI AAT'ATT
Cuts at: 5 52 4072 5
Size: 47 4020 2081
Fragments arranged by size:
4020 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6148
StyI C'CwwG_G
Cuts at: 513 1065 2004 2958 3153 3587 513
Size: 552 939 954 195 434 3074
Fragments arranged by size:
3074 954 939 552 434 195
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2323 4175 4192 4345 4530 4975 325
Size: 1255 743 1852 17 153 185 445 1498
Fragments arranged by size:
1852 1498 1255 743 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2439 2992 3348
Size: 274 80 33 51 1443 462 553 356
Cuts at: 3348 4394 6106 96
Size: 1046 1712 138
Fragments arranged by size:
1712 1443 1046 553 462 356 274 138
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3149 3181 6108 6140 3149
Size: 32 2927 32 3157
Fragments arranged by size:
3157 2927 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2642 1316
Size: 1326 4822
XbaI T'CTAG_A
Cuts at: 3401 3484 3401
Size: 83 6065
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2601 2601
Size: 6148
XhoI C'TCGA_G
Cuts at: 1943 2123 1943
Size: 180 5968
XmaI C'CCGG_G
Cuts at: 1870 3473 1870
Size: 1603 4545
XmnI GAAnn'nnTTC
Cuts at: 4277 4277
Size: 6148
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3956 276
Size: 53 83 186 936 2422 2468
Fragments arranged by size:
2468 2422 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII
BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI FalI FseI FspI HaeII Hin4I HincII
HindIII HpaI KasI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI
NotI NsiI NspI PasI PciI PflMI PmeI PpiI
PshAI PsiI PspOMI PspXI PsrI PstI PvuI PvuII
SacI SalI SapI SbfI ScaI SexAI SfcI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI AscI AvrII BaeI BplI BsiWI BsmBI BstXI
BstZ17I DraIII EcoNI EcoRV FspAI NruI PacI PfoI
PmlI PpuMI RsrII SacII SanDI SfiI SrfI SwaI