Restriction map for
pFN21AB5834
(Linear) (Six-base) MAPSORT of: KIBB5834.seq Check: 8269 from: 1 to: 1443
KIBB5834.orf
With 182 enzymes: SgfI *
July 15, 2010 18:27 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 302 1443
Size: 302 1141
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1066 1443
Size: 1066 377
AfeI AGC'GCT
Cuts at: 0 46 286 499 1443
Size: 46 240 213 944
Fragments arranged by size:
944 240 213 46
AhdI GACnn_n'nnGTC
Cuts at: 0 312 963 1443
Size: 312 651 480
Fragments arranged by size:
651 480 312
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1179 1443
Size: 1179 264
AlwNI CAG_nnn'CTG
Cuts at: 0 339 582 682 1044 1443
Size: 339 243 100 362 399
Fragments arranged by size:
399 362 339 243 100
ApaI G_GGCC'C
Cuts at: 0 570 1282 1443
Size: 570 712 161
Fragments arranged by size:
712 570 161
AvaI C'yCGr_G
Cuts at: 0 118 1139 1172 1443
Size: 118 1021 33 271
Fragments arranged by size:
1021 271 118 33
BamHI G'GATC_C
Cuts at: 0 454 1074 1443
Size: 454 620 369
Fragments arranged by size:
620 454 369
BanI G'GyrC_C
Cuts at: 0 253 378 1163 1443
Size: 253 125 785 280
Fragments arranged by size:
785 280 253 125
BanII G_rGCy'C
Cuts at: 0 148 169 251 570 863 896 1148 1199
Size: 148 21 82 319 293 33 252 51
Cuts at: 1199 1282 1394 1438 1443
Size: 83 112 44 5
Fragments arranged by size:
319 293 252 148 112 83 82 51
44 33 21 5
BbeI G_GCGC'C
Cuts at: 0 257 382 1443
Size: 257 125 1061
Fragments arranged by size:
1061 257 125
BbsI GAAGACnn'nnnn_
Cuts at: 0 76 1443
Size: 76 1367
BbvCI CC'TCA_GC
Cuts at: 0 526 1443
Size: 526 917
BfrBI ATG'CAT
Cuts at: 0 1156 1443
Size: 1156 287
BglI GCCn_nnn'nGGC
Cuts at: 0 512 677 1443
Size: 512 165 766
Fragments arranged by size:
766 512 165
BlpI GC'TnA_GC
Cuts at: 0 281 890 1443
Size: 281 609 553
Fragments arranged by size:
609 553 281
Bme1580I G_kGCm'C
Cuts at: 0 570 785 1282 1443
Size: 570 215 497 161
Fragments arranged by size:
570 497 215 161
BmgBI CAC'GTC
Cuts at: 0 965 1443
Size: 965 478
BmrI ACTGGGnnnn_n'
Cuts at: 0 1102 1427 1443
Size: 1102 325 16
Fragments arranged by size:
1102 325 16
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 433 442 604 1443
Size: 433 9 162 839
Fragments arranged by size:
839 433 162 9
Bpu10I CC'TnA_GC
Cuts at: 0 245 398 526 971 1443
Size: 245 153 128 445 472
Fragments arranged by size:
472 445 245 153 128
BsaI GGTCTCn'nnnn_
Cuts at: 0 229 1443
Size: 229 1214
BsaAI yAC'GTr
Cuts at: 0 522 1443
Size: 522 921
BsaHI Gr'CG_yC
Cuts at: 0 254 379 1443
Size: 254 125 1064
Fragments arranged by size:
1064 254 125
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 971 1001 1443
Size: 971 30 442
Fragments arranged by size:
971 442 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 853 1198 1443
Size: 853 345 245
Fragments arranged by size:
853 345 245
BseYI C'CCAG_C
Cuts at: 0 876 1281 1379 1443
Size: 876 405 98 64
Fragments arranged by size:
876 405 98 64
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 341 352 496 1443
Size: 341 11 144 947
Fragments arranged by size:
947 341 144 11
BsiEI CG_ry'CG
Cuts at: 0 305 663 1443
Size: 305 358 780
Fragments arranged by size:
780 358 305
BsiHKAI G_wGCw'C
Cuts at: 0 86 148 169 863 1199 1249 1443
Size: 86 62 21 694 336 50 194
Fragments arranged by size:
694 336 194 86 62 50 21
Bsp1286I G_dGCh'C
Cuts at: 0 86 148 169 251 570 785 863 896
Size: 86 62 21 82 319 215 78 33
Cuts at: 896 1148 1199 1249 1282 1394 1438 1443
Size: 252 51 50 33 112 44 5
Fragments arranged by size:
319 252 215 112 86 82 78 62
51 50 44 33 33 21 5
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 302 1443
Size: 302 1141
BsrBI CCG'CTC
Cuts at: 0 1364 1443
Size: 1364 79
BsrDI GCAATG_nn'
Cuts at: 0 911 1443
Size: 911 532
BsrFI r'CCGG_y
Cuts at: 0 658 749 1443
Size: 658 91 694
Fragments arranged by size:
694 658 91
BsrGI T'GTAC_A
Cuts at: 0 434 1343 1443
Size: 434 909 100
Fragments arranged by size:
909 434 100
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1041 1443
Size: 1041 402
BstYI r'GATC_y
Cuts at: 0 454 1074 1443
Size: 454 620 369
Fragments arranged by size:
620 454 369
Bsu36I CC'TnA_GG
Cuts at: 0 675 1094 1371 1443
Size: 675 419 277 72
Fragments arranged by size:
675 419 277 72
BtgI C'CryG_G
Cuts at: 0 102 230 560 1443
Size: 102 128 330 883
Fragments arranged by size:
883 330 128 102
BtsI GCAGTG_nn'
Cuts at: 0 17 257 275 1320 1443
Size: 17 240 18 1045 123
Fragments arranged by size:
1045 240 123 18 17
EaeI y'GGCC_r
Cuts at: 0 92 156 302 627 660 1443
Size: 92 64 146 325 33 783
Fragments arranged by size:
783 325 146 92 64 33
EagI C'GGCC_G
Cuts at: 0 302 660 1443
Size: 302 358 783
Fragments arranged by size:
783 358 302
EarI CTCTTCn'nnn_
Cuts at: 0 1354 1443
Size: 1354 89
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 256 1443
Size: 256 1187
EcoICRI GAG'CTC
Cuts at: 0 146 167 861 1197 1443
Size: 146 21 694 336 246
Fragments arranged by size:
694 336 246 146 21
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 433 442 604 1066 1443
Size: 433 9 162 462 377
Fragments arranged by size:
462 433 377 162 9
EcoO109I rG'GnC_Cy
Cuts at: 0 566 679 1098 1122 1278 1367 1443
Size: 566 113 419 24 156 89 76
Fragments arranged by size:
566 419 156 113 89 76 24
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 43 1443
Size: 43 1400
FseI GG_CCGG'CC
Cuts at: 0 662 1443
Size: 662 781
FspI TGC'GCA
Cuts at: 0 910 1031 1443
Size: 910 121 412
Fragments arranged by size:
910 412 121
HaeII r_GCGC'y
Cuts at: 0 48 257 288 382 501 1443
Size: 48 209 31 94 119 942
Fragments arranged by size:
942 209 119 94 48 31
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 298 330 949 981 1443
Size: 298 32 619 32 462
Fragments arranged by size:
619 462 298 32 32
HindIII A'AGCT_T
Cuts at: 0 400 1443
Size: 400 1043
KasI G'GCGC_C
Cuts at: 0 253 378 1443
Size: 253 125 1065
Fragments arranged by size:
1065 253 125
MslI CAynn'nnrTG
Cuts at: 0 1161 1443
Size: 1161 282
MspA1I CmG'CkG
Cuts at: 0 368 668 1443
Size: 368 300 775
Fragments arranged by size:
775 368 300
NaeI GCC'GGC
Cuts at: 0 660 1443
Size: 660 783
NarI GG'CG_CC
Cuts at: 0 254 379 1443
Size: 254 125 1064
Fragments arranged by size:
1064 254 125
NgoMIV G'CCGG_C
Cuts at: 0 658 1443
Size: 658 785
NsiI A_TGCA'T
Cuts at: 0 1158 1443
Size: 1158 285
NspI r_CATG'y
Cuts at: 0 1030 1443
Size: 1030 413
PasI CC'CwG_GG
Cuts at: 0 762 1119 1275 1443
Size: 762 357 156 168
Fragments arranged by size:
762 357 168 156
PflMI CCAn_nnn'nTGG
Cuts at: 0 62 1443
Size: 62 1381
PshAI GACnn'nnGTC
Cuts at: 0 1420 1443
Size: 1420 23
PspOMI G'GGCC_C
Cuts at: 0 566 1278 1443
Size: 566 712 165
Fragments arranged by size:
712 566 165
PspXI vC'TCGA_Gb
Cuts at: 0 118 1443
Size: 118 1325
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 418 450 1443
Size: 418 32 993
Fragments arranged by size:
993 418 32
PvuII CAG'CTG
Cuts at: 0 368 1443
Size: 368 1075
SacI G_AGCT'C
Cuts at: 0 148 169 863 1199 1443
Size: 148 21 694 336 244
Fragments arranged by size:
694 336 244 148 21
SapI GCTCTTCn'nnn_
Cuts at: 0 1354 1443
Size: 1354 89
SexAI A'CCwGG_T
Cuts at: 0 1296 1443
Size: 1296 147
SfoI GGC'GCC
Cuts at: 0 255 380 1443
Size: 255 125 1063
Fragments arranged by size:
1063 255 125
SmlI C'TyrA_G
Cuts at: 0 118 1443
Size: 118 1325
SphI G_CATG'C
Cuts at: 0 1030 1443
Size: 1030 413
StyI C'CwwG_G
Cuts at: 0 953 1148 1443
Size: 953 195 295
Fragments arranged by size:
953 295 195
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 318 1443
Size: 318 1125
TatI w'GTAC_w
Cuts at: 0 434 987 1343 1443
Size: 434 553 356 100
Fragments arranged by size:
553 434 356 100
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1144 1176 1443
Size: 1144 32 267
Fragments arranged by size:
1144 267 32
Tth111I GACn'n_nGTC
Cuts at: 0 637 1443
Size: 637 806
XbaI T'CTAG_A
Cuts at: 0 1396 1443
Size: 1396 47
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 596 1443
Size: 596 847
XhoI C'TCGA_G
Cuts at: 0 118 1443
Size: 118 1325
Enzymes that do cut:
AarI AcuI AfeI AhdI AlfI AlwNI ApaI AvaI
BamHI BanI BanII BbeI BbsI BbvCI BfrBI BglI
BlpI Bme1580I BmgBI BmrI BpmI Bpu10I BsaI BsaAI
BsaHI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I
BspMI BsrBI BsrDI BsrFI BsrGI BstAPI BstYI Bsu36I
BtgI BtsI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I FalI FseI FspI HaeII Hin4I HindIII KasI
MslI MspA1I NaeI NarI NgoMIV NsiI NspI PasI
PflMI PshAI PspOMI PspXI PsrI PvuII SacI SapI
SexAI SfoI SmlI SphI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI
Enzymes that do not cut:
AatII AccI Acc65I AclI AflII AflIII AgeI AleI
AloI ApaLI ApoI AscI AseI AsiSI AvrII BaeI
BcgI BciVI BclI BglII BmtI BplI BpuEI BsaBI
BsaWI BsiWI BsmI BsmBI BspEI BspHI BssHII BssSI
BstBI BstEII BstXI BstZ17I BtgZI ClaI CspCI DraI
DraIII DrdI EcoNI EcoRI EcoRV FspAI HincII HpaI
KpnI MfeI MluI MmeI MscI NcoI NdeI NheI
NotI NruI PacI PciI PfoI PmeI PmlI PpiI
PpuMI PsiI PstI PvuI RsrII SacII SalI SanDI
SbfI ScaI SfcI SfiI SgfI SgrAI SmaI SnaBI
SpeI SrfI SspI StuI SwaI XmaI XmnI ZraI