################################## # NO CUT ENZYMES ( 132 enzymes) # ################################## AatII Acc65I AccI AclI AcsI AflII AgeI AhdI Alw44I AocI Aor51HI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AspI AvrII BamHI BbrPI BbsI BbvCI BcgI BclI BfrI BglII BlnI BlpI Bpu1102I BpuAI BsaAI BsaBI BsaI BsiHKAI BsiWI BsmAI BsmBI BsmI Bsp1407I BspHI BspLU11I BsrDI BsrGI BsrI BssHII Bst1107I BstBI BstEII BstPI BstZ17I Bsu36I CelII CpoI DraI Eam1105I Eco47III Eco81I EcoNI EcoO65I EcoRI EcoRV EcoT22I FbaI FseI HincII HindII HindIII HpaI KpnI KspI MaeIII MamI MfeI MseI MslI MunI NcoI NdeI NheI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI PvuII RcaI RsrII SacI SacII SalI SbfI ScaI SexAI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI SwaI TfiI Tru9I Tsp45I Tsp509I Tth111I Van91I XbaI XcmI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 38 enzymes) # ################################### AccIII 217 TCCGGA T^CCGGA AfaI 540 GTAC GT^AC AflIII 710 ACGCGT A^C[GA][CT]GT BalI 569 TGGCCA TGG^CCA BciVI 157 GGATAC GTATCC(6/5) BpmI 508 CTGGAG CTGGAG(16/14) BseAI 217 TCCGGA T^CCGGA BseRI 652 GAGGAG GAGGAG(10/8) BsgI 760 CTGCAC GTGCAG(16/14) BspDI 61 ATCGAT AT^CGAT BspEI 217 TCCGGA T^CCGGA BspMI 791 ACCTGC ACCTGC(4/8) BssSI 40 CACGAG CACGAG(-5/-1) BstAPI 786 GCAGAACCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstXI 123 CCAGGGTCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG BstYI 68 AGATCC [GA]^GATC[CT] ClaI 61 ATCGAT AT^CGAT DdeI 73 CTGAG C^T[ACGT]AG DraII 307 GGGCCCT [GA]G^G[ACGT]CC[CT] DraIII 627 CACCAAGTG CAC[ACGT][ACGT][ACGT]^GTG DrdI 592 GACGCACAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EarI 775 CTCTTC CTCTTC(1/4) EcoO109I 307 GGGCCCT [GA]G^G[ACGT]CC[CT] HinfI 344 GACTC G^A[ACGT]TC HphI 347 TCACC GGTGA(8/7) Ksp632I 775 CTCTTC CTCTTC(1/4) MflI 68 AGATCC [GA]^GATC[CT] MluI 710 ACGCGT A^CGCGT MluNI 569 TGGCCA TGG^CCA MroI 217 TCCGGA T^CCGGA MscI 569 TGGCCA TGG^CCA PleI 344 GACTC GAGTC(4/5) RsaI 540 GTAC GT^AC SapI 774 GCTCTTC GCTCTTC(1/4) SfiI 722 GGCCGCTGCGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC TaqI 62 TCGA T^CGA TthHB8I 62 TCGA T^CGA XhoII 68 AGATCC [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 20 enzymes) # ################################### AluI 52 AGCT AG^CT 214 AGCT AlwI 69 GATCC GGATC(4/5) 240 GATCC ApaI 225 GGGCCC GGGCC^C 307 GGGCCC AviII 252 TGCGCA TGC^GCA 581 TGCGCA BfaI 103 CTAG C^TAG 337 CTAG BsaWI 217 TCCGGA [AT]^CCGG[AT] 452 ACCGGA BstF5I 11 GGATG GGATG(2/0) 359 GGATG DsaI 162 CCACGG C^C[GA][CT]GG 718 CCACGG EcoT14I 383 CCAAGG C^C[AT][AT]GG 572 CCAAGG FokI 11 GGATG GGATG(9/13) 359 GGATG FspI 252 TGCGCA TGC^GCA 581 TGCGCA MaeI 103 CTAG C^TAG 337 CTAG MaeII 484 ACGT A^CGT 607 ACGT MboII 471 GAAGA GAAGA(8/7) 776 TCTTC NaeI 187 GCCGGC GCC^GGC 683 GCCGGC NgoMIV 187 GCCGGC G^CCGGC 683 GCCGGC NlaIII 130 CATG CATG^ 585 CATG NotI 368 GCGGCCGC GC^GGCCGC 729 GCGGCCGC StyI 383 CCAAGG C^C[AT][AT]GG 572 CCAAGG TspRI 299 CAGTG CA[GC]TG[ACGT][ACGT]^ 827 CAGTG ######################################### # 3 OR MORE CUT ENZYMES ( 68 enzymes) # ######################################### AccII AciI AcyI AlwNI AvaI AvaII BanI BanII BbeI BbvI BcnI BglI BmyI BsaHI BsaJI BsiEI BsiYI BslI BsmFI BsoBI Bsp1286I BsrBI BsrFI BssKI BstNI BstUI Cac8I CfoI Cfr10I Cfr13I DpnII EaeI EagI EclXI Eco52I EcoRII FauI Fnu4HI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HpaII ItaI KasI MboI MnlI MspA1I MspI MvaI MvnI MwoI NarI NciI NdeII NlaIV PstI Sau3AI Sau96I ScrFI SfaNI SfcI SfoI StuI TseI