(Circular) (Six-base) MAPSORT of: pFN21AB7578.seq Check: 2592 from: 1 to: 5557 pFN21AB7578 5557 bp With 182 enzymes: SgfI * December 28, 2011 15:26 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3367 278 Size: 53 83 186 936 1831 2468 Fragments arranged by size: 2468 1831 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2900 1937 Size: 963 4594 Acc65I G'GTAC_C Cuts at: 2878 2878 Size: 5557 AclI AA'CG_TT Cuts at: 1563 3684 4057 1563 Size: 2121 373 3063 Fragments arranged by size: 3063 2121 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2814 3620 5004 5272 1632 Size: 1182 806 1384 268 1917 Fragments arranged by size: 1917 1384 1182 806 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5360 AflIII A'CryG_T Cuts at: 1203 2715 4456 5433 1203 Size: 1512 1741 977 1327 Fragments arranged by size: 1741 1512 1327 977 AgeI A'CCGG_T Cuts at: 4388 4388 Size: 5557 AhdI GACnn_n'nnGTC Cuts at: 4286 4286 Size: 5557 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5557 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5557 AlwNI CAG_nnn'CTG Cuts at: 2077 2309 2512 2797 3300 4872 2077 Size: 232 203 285 503 1572 2762 Fragments arranged by size: 2762 1572 503 285 232 203 ApaI G_GGCC'C Cuts at: 2234 2316 2234 Size: 82 5475 ApaLI G'TGCA_C Cuts at: 3614 4770 3614 Size: 1156 4401 ApoI r'AATT_y Cuts at: 1493 2866 3092 3244 5353 1493 Size: 1373 226 152 2109 1697 Fragments arranged by size: 2109 1697 1373 226 152 AseI AT'TA_AT Cuts at: 160 1032 4111 160 Size: 872 3079 1606 Fragments arranged by size: 3079 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5557 AvaI C'yCGr_G Cuts at: 1870 1943 2155 2176 2317 2882 1870 Size: 73 212 21 141 565 4545 Fragments arranged by size: 4545 565 212 141 73 21 BamHI G'GATC_C Cuts at: 2887 3254 2887 Size: 367 5190 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2296 2330 2643 2676 2878 Size: 325 217 627 509 34 313 33 202 Cuts at: 2878 4333 618 Size: 1455 1842 Fragments arranged by size: 1842 1455 627 509 325 313 217 202 34 33 BanII G_rGCy'C Cuts at: 1391 2234 2316 2844 2876 1391 Size: 843 82 528 32 4072 Fragments arranged by size: 4072 843 528 82 32 BbeI G_GCGC'C Cuts at: 2300 2334 2647 2300 Size: 34 313 5210 Fragments arranged by size: 5210 313 34 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4586 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3748 3782 1903 Size: 34 1811 34 3678 Fragments arranged by size: 3678 1811 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2156 3449 4659 2156 Size: 1293 1210 3054 Fragments arranged by size: 3054 1293 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 5557 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2238 2439 2734 Size: 107 122 71 631 605 566 201 295 Cuts at: 2734 4168 136 Size: 1434 1525 Fragments arranged by size: 1525 1434 631 605 566 295 201 122 107 71 BglII A'GATC_T Cuts at: 5552 5552 Size: 5557 BlpI GC'TnA_GC Cuts at: 2974 2974 Size: 5557 Bme1580I G_kGCm'C Cuts at: 1790 2234 2316 3618 4774 1790 Size: 444 82 1302 1156 2573 Fragments arranged by size: 2573 1302 1156 444 82 BmgBI CAC'GTC Cuts at: 1318 2490 1318 Size: 1172 4385 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3288 4246 442 Size: 416 53 83 399 24 1871 958 1753 Fragments arranged by size: 1871 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5557 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2534 4217 1239 Size: 1295 1683 2579 Fragments arranged by size: 2579 1683 1295 Bpu10I CC'TnA_GC Cuts at: 2078 2078 Size: 5557 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3682 4547 4845 3682 Size: 865 298 4394 Fragments arranged by size: 4394 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4220 882 Size: 617 2721 2219 Fragments arranged by size: 2721 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3348 493 Size: 711 2144 2702 Fragments arranged by size: 2702 2144 711 BsaBI GATnn'nnATC Cuts at: 5551 5551 Size: 5557 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2297 2331 2644 3364 Size: 53 83 186 936 764 34 313 720 Cuts at: 3364 3746 4381 275 Size: 382 635 1451 Fragments arranged by size: 1451 936 764 720 635 382 313 186 83 53 34 BsaWI w'CCGG_w Cuts at: 2222 2457 3990 4388 4662 4809 5220 2222 Size: 235 1533 398 274 147 411 2559 Fragments arranged by size: 2559 1533 411 398 274 235 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2019 2049 1178 Size: 30 165 30 616 30 4686 Fragments arranged by size: 4686 616 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2672 1186 Size: 1486 4071 BseYI C'CCAG_C Cuts at: 2020 2288 4760 5256 2020 Size: 268 2472 496 2321 Fragments arranged by size: 2472 2321 496 268 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2750 1163 Size: 573 1014 3970 Fragments arranged by size: 3970 1014 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2377 2729 2738 3007 3768 4796 Size: 275 186 376 352 9 269 761 1028 Cuts at: 4796 1540 Size: 2301 Fragments arranged by size: 2301 1028 761 376 352 275 269 186 9 BsiHKAI G_wGCw'C Cuts at: 3618 3703 4774 3618 Size: 85 1071 4401 Fragments arranged by size: 4401 1071 85 BsmI GAATG_Cn' Cuts at: 3068 3161 3068 Size: 93 5464 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2234 2316 2844 2876 3618 3703 4774 Size: 399 444 82 528 32 742 85 1071 Cuts at: 4774 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 528 444 399 85 82 32 BspEI T'CCGG_A Cuts at: 2222 5220 2222 Size: 2998 2559 BspHI T'CATG_A Cuts at: 3446 5176 3446 Size: 1730 3827 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2805 2912 844 Size: 1961 107 3489 Fragments arranged by size: 3489 1961 107 BsrBI CCG'CTC Cuts at: 1394 1823 2082 2502 2524 2674 3444 1394 Size: 429 259 420 22 150 770 3507 Fragments arranged by size: 3507 770 429 420 259 150 22 BsrDI GCAATG_nn' Cuts at: 65 1248 4052 4226 65 Size: 1183 2804 174 1396 Fragments arranged by size: 2804 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2192 2394 2688 4201 4388 1700 Size: 110 382 202 294 1513 187 2869 Fragments arranged by size: 2869 1513 382 294 202 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5557 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5557 BssSI C'ACGA_G Cuts at: 2045 3617 4629 2045 Size: 1572 1012 2973 Fragments arranged by size: 2973 1572 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2797 794 Size: 2003 3554 BstBI TT'CG_AA Cuts at: 4361 4361 Size: 5557 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5557 BstXI CCAn_nnnn'nTGG Cuts at: 2135 2135 Size: 5557 BstYI r'GATC_y Cuts at: 1972 2073 2887 3254 3639 3656 5108 5194 5206 Size: 101 814 367 385 17 1452 86 12 Cuts at: 5206 5552 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 814 385 367 346 101 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2167 2723 513 Size: 552 939 163 556 3347 Fragments arranged by size: 3347 939 556 552 163 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4818 Fragments arranged by size: 4818 683 56 BtsI GCAGTG_nn' Cuts at: 2310 3073 3867 3887 2310 Size: 763 794 20 3980 Fragments arranged by size: 3980 794 763 20 ClaI AT'CG_AT Cuts at: 1554 2067 1554 Size: 513 5044 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5522 DraI TTT'AAA Cuts at: 2861 2861 Size: 5557 DraIII CAC_nnn'GTG Cuts at: 2637 2637 Size: 5557 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2603 4564 809 Size: 507 1287 1961 1802 Fragments arranged by size: 1961 1802 1287 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2374 2574 2726 Size: 54 1425 50 275 18 544 200 152 Cuts at: 2726 2735 3004 3893 5522 8 Size: 9 269 889 1629 43 Fragments arranged by size: 1629 1425 889 544 275 269 200 152 54 50 43 18 9 EagI C'GGCC_G Cuts at: 1537 1812 2374 2726 2735 3004 1537 Size: 275 562 352 9 269 4090 Fragments arranged by size: 4090 562 352 275 269 9 EarI CTCTTCn'nnn_ Cuts at: 1353 2786 3487 1353 Size: 1433 701 3423 Fragments arranged by size: 3423 1433 701 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2669 4144 4518 4664 387 Size: 1064 138 1080 1475 374 146 1280 Fragments arranged by size: 1475 1280 1080 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2534 2814 3620 4217 5004 5272 1239 Size: 393 902 280 806 597 787 268 1524 Fragments arranged by size: 1524 902 806 787 597 393 280 268 EcoO109I rG'GnC_Cy Cuts at: 2313 2313 Size: 5557 EcoRI G'AATT_C Cuts at: 2866 3244 2866 Size: 378 5179 FspI TGC'GCA Cuts at: 2259 2588 4063 2259 Size: 329 1475 3753 Fragments arranged by size: 3753 1475 329 HaeII r_GCGC'y Cuts at: 1678 2300 2334 2530 2573 2647 4704 1678 Size: 622 34 196 43 74 2057 2531 Fragments arranged by size: 2531 2057 622 196 74 43 34 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2008 2040 4198 4230 4272 Size: 32 135 32 436 32 2158 32 42 Cuts at: 4272 4304 1373 Size: 32 2626 Fragments arranged by size: 2626 2158 436 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2901 3147 669 Size: 988 281 963 246 3079 Fragments arranged by size: 3079 988 963 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5557 HpaI GTT'AAC Cuts at: 3147 3147 Size: 5557 KasI G'GCGC_C Cuts at: 2296 2330 2643 2296 Size: 34 313 5210 Fragments arranged by size: 5210 313 34 KpnI G_GTAC'C Cuts at: 2882 2882 Size: 5557 MfeI C'AATT_G Cuts at: 3156 3156 Size: 5557 MluI A'CGCG_T Cuts at: 2715 5433 2715 Size: 2718 2839 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2499 4671 4855 1305 Size: 1194 2172 184 2007 Fragments arranged by size: 2172 2007 1194 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2576 10 Size: 54 1425 343 744 2991 Fragments arranged by size: 2991 1425 744 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3515 3874 4033 518 Size: 648 2349 359 159 2042 Fragments arranged by size: 2349 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2209 2269 2288 2373 2731 2743 2973 3650 Size: 603 60 19 85 358 12 230 677 Cuts at: 3650 4798 5043 1606 Size: 1148 245 2120 Fragments arranged by size: 2120 1148 677 603 358 245 230 85 60 19 12 NaeI GCC'GGC Cuts at: 2194 2690 2194 Size: 496 5061 NarI GG'CG_CC Cuts at: 2297 2331 2644 2297 Size: 34 313 5210 Fragments arranged by size: 5210 313 34 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4066 Fragments arranged by size: 4066 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5557 NgoMIV G'CCGG_C Cuts at: 2192 2688 2192 Size: 496 5061 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5557 NotI GC'GGCC_GC Cuts at: 2374 2735 3004 2374 Size: 361 269 4927 Fragments arranged by size: 4927 361 269 NspI r_CATG'y Cuts at: 1132 1229 2915 4460 1132 Size: 97 1686 1545 2229 Fragments arranged by size: 2229 1686 1545 97 PasI CC'CwG_GG Cuts at: 1348 2096 2805 1348 Size: 748 709 4100 Fragments arranged by size: 4100 748 709 PciI A'CATG_T Cuts at: 4456 4456 Size: 5557 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5557 PmeI GTTT'AAAC Cuts at: 2861 2861 Size: 5557 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3626 3658 5139 5171 3626 Size: 32 1481 32 4012 Fragments arranged by size: 4012 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5557 PsiI TTA'TAA Cuts at: 3127 3127 Size: 5557 PspOMI G'GGCC_C Cuts at: 2230 2312 2230 Size: 82 5475 PstI C_TGCA'G Cuts at: 830 2305 2709 2793 2802 2909 830 Size: 1475 404 84 9 107 3478 Fragments arranged by size: 3478 1475 404 107 84 9 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5557 SalI G'TCGA_C Cuts at: 1936 2899 1936 Size: 963 4594 SapI GCTCTTCn'nnn_ Cuts at: 2786 2786 Size: 5557 SbfI CC_TGCA'GG Cuts at: 2909 2909 Size: 5557 ScaI AGT'ACT Cuts at: 3805 5517 3805 Size: 1712 3845 SfcI C'TryA_G Cuts at: 826 1045 2301 2705 2789 2798 2905 4040 4721 Size: 219 1256 404 84 9 107 1135 681 Cuts at: 4721 4912 826 Size: 191 1471 Fragments arranged by size: 1471 1256 1135 681 404 219 191 107 84 9 SfiI GGCCn_nnn'nGGCC Cuts at: 2734 2734 Size: 5557 SfoI GGC'GCC Cuts at: 2298 2332 2645 2298 Size: 34 313 5210 Fragments arranged by size: 5210 313 34 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5557 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5557 SmaI CCC'GGG Cuts at: 1872 2884 1872 Size: 1012 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3661 4562 4824 820 Size: 197 926 1718 901 262 1553 Fragments arranged by size: 1718 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3348 493 Size: 2855 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5557 SphI G_CATG'C Cuts at: 1132 2915 1132 Size: 1783 3774 SspI AAT'ATT Cuts at: 5 52 3481 5 Size: 47 3429 2081 Fragments arranged by size: 3429 2081 47 StuI AGG'CCT Cuts at: 1517 2112 2340 2582 1517 Size: 595 228 242 4492 Fragments arranged by size: 4492 595 242 228 StyI C'CwwG_G Cuts at: 513 1065 2004 2388 2577 2996 513 Size: 552 939 384 189 419 3074 Fragments arranged by size: 3074 939 552 419 384 189 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2609 3584 3601 3754 3939 4384 325 Size: 1255 1029 975 17 153 185 445 1498 Fragments arranged by size: 1498 1255 1029 975 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3803 5515 96 Size: 274 80 33 51 1443 1826 1712 138 Fragments arranged by size: 1826 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2019 2051 5517 5549 2019 Size: 32 3466 32 2027 Fragments arranged by size: 3466 2027 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5557 XbaI T'CTAG_A Cuts at: 2893 2893 Size: 5557 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5557 XmaI C'CCGG_G Cuts at: 1870 2882 1870 Size: 1012 4545 XmnI GAAnn'nnTTC Cuts at: 3686 3686 Size: 5557 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3365 276 Size: 53 83 186 936 1831 2468 Fragments arranged by size: 2468 1831 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PspOMI PstI PvuI SalI SapI SbfI ScaI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbvCI BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I EcoICRI EcoNI EcoRV FalI FseI FspAI NruI NsiI PacI PfoI PmlI PpuMI PspXI PsrI PvuII RsrII SacI SacII SanDI SexAI SrfI SwaI XcmI