################################## # NO CUT ENZYMES ( 144 enzymes) # ################################## AatII Acc65I AccI AccII AclI AcyI AflII AflIII AgeI AhdI Alw44I AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AviII AvrII BalI BamHI BanII BbeI BbrPI BcgI BciVI BfrI BglI BglII BlnI BlpI BmyI Bpu1102I BsaAI BsaBI BsaHI BsaI BsiEI BsiHKAI BsiWI BsmBI BsmI Bsp1286I BspDI BspHI BspLU11I BspMI BsrDI BssHII Bst1107I BstBI BstUI BstZ17I Bsu36I CelII ClaI CpoI DraI DrdI DsaI EaeI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoRV EcoT14I EcoT22I FseI FspI HaeII Hin1I HincII HindII HindIII HpaI KasI KpnI KspI MaeII MamI MfeI MluI MluNI MscI MunI MvnI NarI NcoI NdeI NheI NotI NruI NsiI NspV PacI PaeR7I PflMI PinAI PleI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI PvuII RcaI RsrII SacI SacII SalI SbfI SexAI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspI StuI StyI SwaI Van91I XbaI XcmI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 62 enzymes) # ################################### AccIII 572 TCCGGA T^CCGGA AcsI 337 GAATTC [GA]^AATT[CT] AlwI 228 GATCC GGATC(4/5) AlwNI 241 CAGCCACTG CAG[ACGT][ACGT][ACGT]^CTG ApoI 337 GAATTC [GA]^AATT[CT] AvaI 350 CCCGAG C^[CT]CG[GA]G BanI 297 GGTGCC G^G[CT][GA]CC BbsI 152 GTCTTC GAAGAC(2/6) BclI 648 TGATCA T^GATCA BfaI 197 CTAG C^TAG BpuAI 152 GTCTTC GAAGAC(2/6) BsaWI 572 TCCGGA [AT]^CCGG[AT] BseAI 572 TCCGGA T^CCGGA BsgI 616 GTGCAG GTGCAG(16/14) BsmAI 235 GAGAC GTCTC(1/5) BsmFI 348 GTCCC GGGAC(10/14) BsoBI 350 CCCGAG C^[CT]CG[GA]G Bsp1407I 29 TGTACA T^GTACA BspEI 572 TCCGGA T^CCGGA BsrBI 484 GAGCGG CCGCTC(-3/-3) BsrFI 115 GCCGGC [GA]^CCGG[CT] BsrGI 29 TGTACA T^GTACA BssSI 232 CACGAG CACGAG(-5/-1) BstAPI 200 GCAAGTACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstEII 366 GGTCACC G^GT[ACGT]ACC BstPI 366 GGTCACC G^GT[ACGT]ACC BstXI 372 CCAGCCCACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG BstYI 227 AGATCC [GA]^GATC[CT] CfoI 113 GCGC GCG^C Cfr10I 115 GCCGGC [GA]^CCGG[CT] DraII 578 GGGTCCT [GA]G^G[ACGT]CC[CT] DraIII 144 CACAGAGTG CAC[ACGT][ACGT][ACGT]^GTG EcoO109I 578 GGGTCCT [GA]G^G[ACGT]CC[CT] EcoO65I 366 GGTCACC G^GT[ACGT]ACC EcoRI 337 GAATTC G^AATTC FbaI 648 TGATCA T^GATCA HaeIII 409 GGCC GG^CC HgaI 518 GCGTC GACGC(5/10) HhaI 113 GCGC GCG^C HinP1I 113 GCGC G^CGC HinfI 330 GATTC G^A[ACGT]TC MaeI 197 CTAG C^TAG MflI 227 AGATCC [GA]^GATC[CT] MroI 572 TCCGGA T^CCGGA MseI 224 TTAA T^TAA MslI 654 CATGACAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MspA1I 681 CCGCTG C[AC]G^C[GT]G NaeI 115 GCCGGC GCC^GGC NgoMIV 115 GCCGGC G^CCGGC NspI 612 GCATGT [GA]CATG^[CT] PpuMI 578 GGGTCCT [GA]G^G[AT]CC[CT] PstI 523 CTGCAG CTGCA^G SapI 385 GAAGAGC GCTCTTC(1/4) ScaI 203 AGTACT AGT^ACT SfcI 523 CTGCAG C^T[GA][CT]AG SspBI 29 TGTACA T^GTACA TaqI 66 TCGA T^CGA TfiI 330 GATTC G^A[AT]TC Tru9I 224 TTAA T^TAA Tsp509I 338 AATT ^AATT TthHB8I 66 TCGA T^CGA XhoII 227 AGATCC [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 18 enzymes) # ################################### AspI 265 GACCGGGTC GAC[ACGT]^[ACGT][ACGT]GTC 361 GACCGGGTC BbvCI 607 CCTCAGC CCTCAGC(-5/-2) 556 GCTGAGG BcnI 267 CCGGG CC^[GC]GG 363 CCGGG BpmI 19 CTCCAG CTGGAG(16/14) 481 CTGGAG BsaJI 351 CCGAGG C^C[ACGT][ACGT]GG 405 CCTCGG BstF5I 698 CATCC GGATG(2/0) 129 GGATG BstNI 301 CCTGG CC^[AT]GG 480 CCTGG EcoRII 301 CCTGG ^CC[AT]GG 480 CCTGG FauI 42 CCCGC CCCGC(4/6) 680 CCCGC FokI 698 CATCC GGATG(9/13) 129 GGATG HphI 368 TCACC GGTGA(8/7) 588 TCACC MaeIII 367 GTCAC ^GT[ACGT]AC 587 GTCAC MvaI 301 CCTGG CC^[AT]GG 480 CCTGG NciI 267 CCGGG CC^[GC]GG 363 CCGGG PflFI 265 GACCGGGTC GAC[ACGT]^[ACGT][ACGT]GTC 361 GACCGGGTC SfaNI 130 GATGC GCATC(5/9) 597 GATGC Tsp45I 367 GTCAC ^GT[GC]AC 587 GTCAC Tth111I 265 GACCGGGTC GAC[ACGT]^[ACGT][ACGT]GTC 361 GACCGGGTC ######################################### # 3 OR MORE CUT ENZYMES ( 34 enzymes) # ######################################### AciI AfaI AluI AvaII BbvI BseRI BsiYI BslI BsrI BssKI Cac8I Cfr13I DdeI DpnII EarI Fnu4HI HapII HpaII ItaI Ksp632I MboI MboII MnlI MspI MwoI NdeII NlaIII NlaIV RsaI Sau3AI Sau96I ScrFI TseI TspRI