################################## # NO CUT ENZYMES ( 120 enzymes) # ################################## Acc65I AccI AclI AcsI AflII AflIII AgeI AhdI AlwI AocI Aor51HI ApaI ApoI AscI AseI AsnI Asp718I AspEI AspI AviII AvrII BamHI BanI BbeI BbrPI BbvCI BcgI BclI BfrI BglI BlnI BlpI Bpu1102I BsaAI BsaBI BsaI BsiWI BsmI BspDI BspHI BspLU11I BsrBI BsrFI BssHII Bst1107I BstAPI BstBI BstZ17I Bsu36I CelII Cfr10I ClaI CpoI DraI DraIII DrdI DsaI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoRI EcoT14I EcoT22I FbaI FseI FspI HindIII HinfI HpaI KasI KpnI KspI MamI MluI NaeI NarI NcoI NdeI NgoMIV NotI NruI NsiI NspI NspV PacI PflFI PinAI PleI PmaCI PmeI PmlI PshAI PshBI Psp1406I RcaI RsrII SacI SacII SalI SapI ScaI SexAI SfiI SfoI SfuI SgrAI SnaBI SpeI SphI SspI StuI StyI SwaI TfiI Tth111I XbaI ################################### # ONE CUT ENZYMES ( 58 enzymes) # ################################### AccII 764 CGCG CG^CG AccIII 288 TCCGGA T^CCGGA Alw44I 257 GTGCAC G^TGCAC ApaLI 257 GTGCAC G^TGCAC Asp700I 303 GAAGATCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspHI 257 GTGCAC G[AT]GC[AT]^C BalI 914 TGGCCA TGG^CCA BanII 714 GGGCTC G[GA]GC[CT]^C BglII 305 AGATCT A^GATCT BsaWI 288 TCCGGA [AT]^CCGG[AT] BseAI 288 TCCGGA T^CCGGA BsgI 190 GTGCAG GTGCAG(16/14) BsiEI 803 CGATCG CG[GA][CT]^CG BsiHKAI 257 GTGCAC G[AT]GC[AT]^C Bsp1407I 1004 TGTACA T^GTACA BspEI 288 TCCGGA T^CCGGA BspMI 474 ACCTGC ACCTGC(4/8) BsrGI 1004 TGTACA T^GTACA BstEII 234 GGTCACC G^GT[ACGT]ACC BstPI 234 GGTCACC G^GT[ACGT]ACC BstUI 764 CGCG CG^CG BstXI 527 CCATTGCTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG BstYI 305 AGATCT [GA]^GATC[CT] DraII 217 GGGACCC [GA]G^G[ACGT]CC[CT] EarI 925 CTCTTC CTCTTC(1/4) EcoO109I 217 GGGACCC [GA]G^G[ACGT]CC[CT] EcoO65I 234 GGTCACC G^GT[ACGT]ACC EcoRV 136 GATATC GAT^ATC HincII 439 GTCAAC GT[CT]^[GA]AC HindII 439 GTCAAC GT[CT]^[GA]AC Ksp632I 925 CTCTTC CTCTTC(1/4) MfeI 353 CAATTG C^AATTG MflI 305 AGATCT [GA]^GATC[CT] MluNI 914 TGGCCA TGG^CCA MroI 288 TCCGGA T^CCGGA MscI 914 TGGCCA TGG^CCA MseI 542 TTAA T^TAA MunI 353 CAATTG C^AATTG MvnI 764 CGCG CG^CG NheI 46 GCTAGC G^CTAGC PaeR7I 54 CTCGAG C^TCGAG PflMI 381 CCAGACACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PpuMI 217 GGGACCC [GA]G^G[AT]CC[CT] PstI 838 CTGCAG CTGCA^G PvuI 803 CGATCG CGAT^CG PvuII 193 CAGCTG CAG^CTG SbfI 837 CCTGCAGG CCTGCA^GG SmaI 228 CCCGGG CCC^GGG SmlI 54 CTCGAG C^T[CT][GA]AG Sse8387I 837 CCTGCAGG CCTGCA^GG SspBI 1004 TGTACA T^GTACA Tru9I 542 TTAA T^TAA Van91I 381 CCAGACACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XhoI 54 CTCGAG C^TCGAG XhoII 305 AGATCT [GA]^GATC[CT] XmaCI 228 CCCGGG C^CCGGG XmaI 228 CCCGGG C^CCGGG XmnI 303 GAAGATCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 23 enzymes) # ################################### AatII 102 GACGTC GACGT^C 173 GACGTC AfaI 660 GTAC GT^AC 1005 GTAC AvaI 54 CTCGAG C^[CT]CG[GA]G 228 CCCGGG BciVI 6 GGATAC GTATCC(6/5) 496 GGATAC BfaI 38 CTAG C^TAG 47 CTAG BmyI 257 GTGCAC G[AGT]GC[ACT]^C 714 GGGCTC BseRI 717 CTCCTC GAGGAG(10/8) 859 CTCCTC BsmFI 217 GGGAC GGGAC(10/14) 346 GGGAC BsoBI 54 CTCGAG C^[CT]CG[GA]G 228 CCCGGG Bsp1286I 257 GTGCAC G[AGT]GC[ACT]^C 714 GGGCTC DdeI 167 CTGAG C^T[ACGT]AG 178 CTCAG EaeI 114 TGGCCG [CT]^GGCC[GA] 914 TGGCCA HgaI 348 GACGC GACGC(5/10) 762 GACGC MaeI 38 CTAG C^TAG 47 CTAG MaeII 103 ACGT A^CGT 174 ACGT MaeIII 163 GTCAC ^GT[ACGT]AC 235 GTCAC MspA1I 193 CAGCTG C[AC]G^C[GT]G 910 CCGCTG RsaI 660 GTAC GT^AC 1005 GTAC TaqI 55 TCGA T^CGA 143 TCGA Tsp45I 163 GTCAC ^GT[GC]AC 235 GTCAC Tsp509I 354 AATT ^AATT 959 AATT TthHB8I 55 TCGA T^CGA 143 TCGA XcmI 377 CCAACCAGACACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 779 CCATGCCCATCCTGG ######################################### # 3 OR MORE CUT ENZYMES ( 57 enzymes) # ######################################### AciI AcyI AluI AlwNI AvaII BbsI BbvI BcnI BpmI BpuAI BsaHI BsaJI BsiYI BslI BsmAI BsmBI BsrDI BsrI BssKI BssSI BstF5I BstNI Cac8I CfoI Cfr13I DpnII EcoRII FauI Fnu4HI FokI HaeII HaeIII HapII HhaI Hin1I HinP1I HpaII HphI ItaI MboI MboII MnlI MslI MspI MvaI MwoI NciI NdeII NlaIII NlaIV Sau3AI Sau96I ScrFI SfaNI SfcI TseI TspRI