(Circular) (Six-base) MAPSORT of: pFN21AE6385.seq Check: 1342 from: 1 to: 5725 pFN21AE6385 5725 bp With 182 enzymes: SgfI * March 18, 2015 14:51 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2488 2488 Size: 5725 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2111 2182 3535 278 Size: 53 83 186 936 575 71 1353 2468 Fragments arranged by size: 2468 1353 936 575 186 83 71 53 AccI GT'mk_AC Cuts at: 1937 3068 1937 Size: 1131 4594 Acc65I G'GTAC_C Cuts at: 3046 3046 Size: 5725 AclI AA'CG_TT Cuts at: 1563 3852 4225 1563 Size: 2289 373 3063 Fragments arranged by size: 3063 2289 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2110 3788 5172 5440 1632 Size: 478 1678 1384 268 1917 Fragments arranged by size: 1917 1678 1384 478 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5528 AflIII A'CryG_T Cuts at: 1203 4624 5601 1203 Size: 3421 977 1327 Fragments arranged by size: 3421 1327 977 AgeI A'CCGG_T Cuts at: 4556 4556 Size: 5725 AhdI GACnn_n'nnGTC Cuts at: 4454 4454 Size: 5725 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5725 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5725 AlwNI CAG_nnn'CTG Cuts at: 2203 2392 2419 3468 5040 2203 Size: 189 27 1049 1572 2888 Fragments arranged by size: 2888 1572 1049 189 27 ApaLI G'TGCA_C Cuts at: 2262 3782 4938 2262 Size: 1520 1156 3049 Fragments arranged by size: 3049 1520 1156 ApoI r'AATT_y Cuts at: 1493 3034 3260 3412 5521 1493 Size: 1541 226 152 2109 1697 Fragments arranged by size: 2109 1697 1541 226 152 AseI AT'TA_AT Cuts at: 160 1032 4279 160 Size: 872 3247 1606 Fragments arranged by size: 3247 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5725 AvaI C'yCGr_G Cuts at: 1870 1943 2059 2233 3050 1870 Size: 73 116 174 817 4545 Fragments arranged by size: 4545 817 174 116 73 BamHI G'GATC_C Cuts at: 3055 3422 3055 Size: 367 5358 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3046 4501 618 Size: 325 217 627 1259 1455 1842 Fragments arranged by size: 1842 1455 1259 627 325 217 BanII G_rGCy'C Cuts at: 1391 2723 3044 1391 Size: 1332 321 4072 Fragments arranged by size: 4072 1332 321 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2363 2398 2837 928 Size: 971 464 35 439 3816 Fragments arranged by size: 3816 971 464 439 35 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3916 3950 1903 Size: 34 1979 34 3678 Fragments arranged by size: 3678 1979 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2005 2495 3617 4827 2005 Size: 490 1122 1210 2903 Fragments arranged by size: 2903 1210 1122 490 BclI T'GATC_A Cuts at: 1548 1548 Size: 5725 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4336 136 Size: 107 122 71 631 605 2664 1525 Fragments arranged by size: 2664 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2310 5720 2310 Size: 3410 2315 BlpI GC'TnA_GC Cuts at: 3142 3142 Size: 5725 Bme1580I G_kGCm'C Cuts at: 1790 2266 3786 4942 1790 Size: 476 1520 1156 2573 Fragments arranged by size: 2573 1520 1156 476 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5725 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2093 3456 4414 Size: 416 53 83 399 24 676 1363 958 Cuts at: 4414 442 Size: 1753 Fragments arranged by size: 1753 1363 958 676 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 2055 1056 Size: 999 4726 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2225 2278 2696 2774 2846 4385 1239 Size: 986 53 418 78 72 1539 2579 Fragments arranged by size: 2579 1539 986 418 78 72 53 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3850 4715 5013 3850 Size: 865 298 4562 Fragments arranged by size: 4562 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4388 882 Size: 617 2889 2219 Fragments arranged by size: 2889 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3516 493 Size: 711 2312 2702 Fragments arranged by size: 2702 2312 711 BsaBI GATnn'nnATC Cuts at: 5719 5719 Size: 5725 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2108 2179 2354 3532 Size: 53 83 186 936 575 71 175 1178 Cuts at: 3532 3914 4549 275 Size: 382 635 1451 Fragments arranged by size: 1451 1178 936 635 575 382 186 175 83 71 53 BsaWI w'CCGG_w Cuts at: 2293 4158 4556 4830 4977 5388 2293 Size: 1865 398 274 147 411 2630 Fragments arranged by size: 2630 1865 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5500 Fragments arranged by size: 5500 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2713 2855 1186 Size: 1527 142 4056 Fragments arranged by size: 4056 1527 142 BseYI C'CCAG_C Cuts at: 2581 4928 5424 2581 Size: 2347 496 2882 Fragments arranged by size: 2882 2347 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2216 1163 Size: 573 480 4672 Fragments arranged by size: 4672 573 480 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2811 3175 3936 4964 1540 Size: 275 186 810 364 761 1028 2301 Fragments arranged by size: 2301 1028 810 761 364 275 186 BsiHKAI G_wGCw'C Cuts at: 2266 3786 3871 4942 2266 Size: 1520 85 1071 3049 Fragments arranged by size: 3049 1520 1071 85 BsmI GAATG_Cn' Cuts at: 3236 3329 3236 Size: 93 5632 BsmBI CGTCTCn'nnnn_ Cuts at: 2170 2186 2533 2750 2170 Size: 16 347 217 5145 Fragments arranged by size: 5145 347 217 16 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2266 2723 3044 3786 3871 4942 1391 Size: 399 476 457 321 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 476 457 399 321 85 BspEI T'CCGG_A Cuts at: 2293 5388 2293 Size: 3095 2630 BspHI T'CATG_A Cuts at: 3614 5344 3614 Size: 1730 3995 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2488 3080 844 Size: 1644 592 3489 Fragments arranged by size: 3489 1644 592 BsrBI CCG'CTC Cuts at: 1394 1823 3612 1394 Size: 429 1789 3507 Fragments arranged by size: 3507 1789 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2467 2532 2653 4220 4394 65 Size: 1183 1219 65 121 1567 174 1396 Fragments arranged by size: 1567 1396 1219 1183 174 121 65 BsrFI r'CCGG_y Cuts at: 1700 1810 4369 4556 1700 Size: 110 2559 187 2869 Fragments arranged by size: 2869 2559 187 110 BsrGI T'GTAC_A Cuts at: 96 3009 96 Size: 2913 2812 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5725 BssSI C'ACGA_G Cuts at: 2714 2882 2989 3785 4797 2714 Size: 168 107 796 1012 3642 Fragments arranged by size: 3642 1012 796 168 107 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5725 BstBI TT'CG_AA Cuts at: 4529 4529 Size: 5725 BstEII G'GTnAC_C Cuts at: 1184 2239 1184 Size: 1055 4670 BstXI CCAn_nnnn'nTGG Cuts at: 2539 2539 Size: 5725 BstYI r'GATC_y Cuts at: 1972 2310 3055 3422 3807 3824 5276 5362 5374 Size: 338 745 367 385 17 1452 86 12 Cuts at: 5374 5720 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 745 385 367 346 338 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 4234 Fragments arranged by size: 4234 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2079 2718 487 Size: 683 56 853 639 3494 Fragments arranged by size: 3494 853 683 639 56 BtsI GCAGTG_nn' Cuts at: 2420 3241 4035 4055 2420 Size: 821 794 20 4090 Fragments arranged by size: 4090 821 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5725 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5690 DraI TTT'AAA Cuts at: 3029 3029 Size: 5725 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4732 809 Size: 507 3416 1802 Fragments arranged by size: 3416 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2119 2919 3172 Size: 54 1425 50 275 18 289 800 253 Cuts at: 3172 4061 5690 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 800 289 275 253 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3172 1537 Size: 275 1360 4090 Fragments arranged by size: 4090 1360 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2936 3655 1353 Size: 1583 719 3423 Fragments arranged by size: 3423 1583 719 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2024 4312 4686 4832 387 Size: 1064 138 435 2288 374 146 1280 Fragments arranged by size: 2288 1280 1064 435 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2110 2225 2278 2696 2774 2846 3788 Size: 393 478 115 53 418 78 72 942 Cuts at: 3788 4385 5172 5440 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 942 787 597 478 418 393 268 115 78 72 53 EcoO109I rG'GnC_Cy Cuts at: 2223 2223 Size: 5725 EcoRI G'AATT_C Cuts at: 3034 3412 3034 Size: 378 5347 EcoRV GAT'ATC Cuts at: 2143 2143 Size: 5725 FspI TGC'GCA Cuts at: 4231 4231 Size: 5725 HaeII r_GCGC'y Cuts at: 1678 2058 2526 2765 4872 1678 Size: 380 468 239 2107 2531 Fragments arranged by size: 2531 2107 468 380 239 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2502 2534 4366 4398 4440 Size: 32 135 32 930 32 1832 32 42 Cuts at: 4440 4472 1373 Size: 32 2626 Fragments arranged by size: 2626 1832 930 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2446 3069 3315 669 Size: 988 281 508 623 246 3079 Fragments arranged by size: 3079 988 623 508 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5725 HpaI GTT'AAC Cuts at: 3315 3315 Size: 5725 KpnI G_GTAC'C Cuts at: 3050 3050 Size: 5725 MfeI C'AATT_G Cuts at: 2358 3324 2358 Size: 966 4759 MluI A'CGCG_T Cuts at: 5601 5601 Size: 5725 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2104 4839 5023 1305 Size: 799 2735 184 2007 Fragments arranged by size: 2735 2007 799 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2921 10 Size: 54 1425 343 1089 2814 Fragments arranged by size: 2814 1425 1089 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2537 2795 2819 3683 4042 4201 518 Size: 648 1371 258 24 864 359 159 2042 Fragments arranged by size: 2042 1371 864 648 359 258 159 24 MspA1I CmG'CkG Cuts at: 1606 2200 2917 3141 3818 4966 5211 1606 Size: 594 717 224 677 1148 245 2120 Fragments arranged by size: 2120 1148 717 677 594 245 224 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4234 Fragments arranged by size: 4234 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5725 NheI G'CTAG_C Cuts at: 1052 2051 1052 Size: 999 4726 NotI GC'GGCC_GC Cuts at: 3172 3172 Size: 5725 NspI r_CATG'y Cuts at: 1132 1229 3083 4628 1132 Size: 97 1854 1545 2229 Fragments arranged by size: 2229 1854 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5725 PciI A'CATG_T Cuts at: 4624 4624 Size: 5725 PflMI CCAn_nnn'nTGG Cuts at: 1108 2392 1108 Size: 1284 4441 PfoI T'CCnGG_A Cuts at: 2694 2694 Size: 5725 PmeI GTTT'AAAC Cuts at: 3029 3029 Size: 5725 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3794 3826 5307 5339 3794 Size: 32 1481 32 4180 Fragments arranged by size: 4180 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2223 2223 Size: 5725 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5725 PsiI TTA'TAA Cuts at: 3295 3295 Size: 5725 PspXI vC'TCGA_Gb Cuts at: 2059 2059 Size: 5725 PstI C_TGCA'G Cuts at: 830 2847 3077 830 Size: 2017 230 3478 Fragments arranged by size: 3478 2017 230 PvuI CG_AT'CG Cuts at: 2001 2811 2001 Size: 810 4915 PvuII CAG'CTG Cuts at: 2200 2200 Size: 5725 SalI G'TCGA_C Cuts at: 1936 3067 1936 Size: 1131 4594 SanDI GG'GwC_CC Cuts at: 2223 2223 Size: 5725 SbfI CC_TGCA'GG Cuts at: 2847 3077 2847 Size: 230 5495 ScaI AGT'ACT Cuts at: 3973 5685 3973 Size: 1712 4013 SfcI C'TryA_G Cuts at: 826 1045 2022 2625 2843 3073 4208 4889 5080 Size: 219 977 603 218 230 1135 681 191 Cuts at: 5080 826 Size: 1471 Fragments arranged by size: 1471 1135 977 681 603 230 219 218 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5725 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5725 SmaI CCC'GGG Cuts at: 1872 2235 3052 1872 Size: 363 817 4545 Fragments arranged by size: 4545 817 363 SmlI C'TyrA_G Cuts at: 820 1017 1943 2059 3829 4730 4992 820 Size: 197 926 116 1770 901 262 1553 Fragments arranged by size: 1770 1553 926 901 262 197 116 SnaBI TAC'GTA Cuts at: 493 3516 493 Size: 3023 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5725 SphI G_CATG'C Cuts at: 1132 3083 1132 Size: 1951 3774 SspI AAT'ATT Cuts at: 5 52 3649 5 Size: 47 3597 2081 Fragments arranged by size: 3597 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5725 StyI C'CwwG_G Cuts at: 513 1065 2004 3164 513 Size: 552 939 1160 3074 Fragments arranged by size: 3074 1160 939 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2357 3752 3769 3922 4107 4552 325 Size: 1255 777 1395 17 153 185 445 1498 Fragments arranged by size: 1498 1395 1255 777 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3009 3971 5683 Size: 274 80 33 51 1443 1032 962 1712 Cuts at: 5683 96 Size: 138 Fragments arranged by size: 1712 1443 1032 962 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2061 2093 5685 5717 2061 Size: 32 3592 32 2069 Fragments arranged by size: 3592 2069 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5725 XbaI T'CTAG_A Cuts at: 3061 3061 Size: 5725 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2389 2791 2389 Size: 402 5323 XhoI C'TCGA_G Cuts at: 1943 2059 1943 Size: 116 5609 XmaI C'CCGG_G Cuts at: 1870 2233 3050 1870 Size: 363 817 4545 Fragments arranged by size: 4545 817 363 XmnI GAAnn'nnTTC Cuts at: 2312 3854 2312 Size: 1542 4183 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2109 2180 3533 276 Size: 53 83 186 936 575 71 1353 2468 Fragments arranged by size: 2468 1353 936 575 186 83 71 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspXI PstI PvuI PvuII SalI SanDI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BstZ17I Bsu36I DraIII EcoICRI EcoNI FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspOMI PsrI RsrII SacI SacII SapI SexAI SfiI SfoI SrfI SwaI