Restriction map for
pFN21AE6394
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# NO CUT ENZYMES ( 151 enzymes) #
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AatII Acc65I AccIII AclI AcsI
AcyI AfaI AflII AflIII AgeI
AhdI Alw44I AlwNI AocI Aor51HI
ApaLI ApoI AscI AseI AsnI
Asp700I Asp718I AspEI AspI AvaI
AviII BamHI BanI BbeI BbrPI
BbsI BcgI BclI BfrI BglI
BglII BlpI Bpu1102I BpuAI BsaAI
BsaHI BsaWI BseAI BsiWI BsmBI
BsmI BsoBI Bsp1407I BspDI BspEI
BspHI BspLU11I BsrDI BsrGI BsrI
BssHII BssSI Bst1107I BstBI BstEII
BstPI BstZ17I Bsu36I CelII ClaI
CpoI DraI DraIII DrdI DsaI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoO65I EcoRI EcoRV EcoT22I
FauI FbaI FseI FspI HaeII
HgaI Hin1I HincII HindII HindIII
HpaI KasI KpnI KspI MaeII
MfeI MluI MroI MseI MunI
NarI NcoI NdeI NheI NotI
NruI NsiI NspV PacI PaeR7I
PflFI PflMI PinAI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PvuI
PvuII RcaI RsaI RsrII SacII
SalI SapI SbfI ScaI SexAI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI Sse8387I SspBI SspI
SwaI TfiI Tru9I Tsp509I Tth111I
Van91I XbaI XhoI XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 38 enzymes) #
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AccI 445 GTCTAC GT^[AC][GT]AC
AccII 99 CGCG CG^CG
AlwI 756 GATCC GGATC(4/5)
ApaI 641 GGGCCC GGGCC^C
BbvCI 226 GCTGAGG CCTCAGC(-5/-2)
BciVI 771 GGATAC GTATCC(6/5)
BsaBI 688 GATGGCGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 648 GGTCTC GGTCTC(1/5)
BsgI 349 CTGCAC GTGCAG(16/14)
BsiEI 95 CGACCG CG[GA][CT]^CG
BspMI 542 ACCTGC ACCTGC(4/8)
BsrBI 261 GAGCGG CCGCTC(-3/-3)
BsrFI 287 GCCGGC [GA]^CCGG[CT]
BstAPI 61 GCACTGCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI 99 CGCG CG^CG
BstYI 755 AGATCC [GA]^GATC[CT]
Cfr10I 287 GCCGGC [GA]^CCGG[CT]
EarI 205 CTCTTC CTCTTC(1/4)
HphI 395 TCACC GGTGA(8/7)
Ksp632I 205 CTCTTC CTCTTC(1/4)
MaeIII 682 GTCAC ^GT[ACGT]AC
MamI 688 GATGGCGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI 755 AGATCC [GA]^GATC[CT]
MslI 855 CACTGATGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 776 CAGCGG C[AC]G^C[GT]G
MvnI 99 CGCG CG^CG
NaeI 287 GCCGGC GCC^GGC
NgoMIV 287 GCCGGC G^CCGGC
SacI 976 GAGCTC GAGCT^C
SfaNI 26 GCATC GCATC(5/9)
SmlI 592 CTCAAG C^T[CT][GA]AG
SphI 294 GCATGC GCATG^C
StuI 509 AGGCCT AGG^CCT
TaqI 153 TCGA T^CGA
Tsp45I 682 GTCAC ^GT[GC]AC
TthHB8I 153 TCGA T^CGA
XcmI 471 CCAGGGAGACTATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 755 AGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 18 enzymes) #
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AvrII 21 CCTAGG C^CTAGG
786 CCTAGG
BalI 734 TGGCCA TGG^CCA
821 TGGCCA
BlnI 21 CCTAGG C^CTAGG
786 CCTAGG
BpmI 865 CTCCAG CTGGAG(16/14)
973 CTGGAG
BsmAI 476 GAGAC GTCTC(1/5)
649 GTCTC
BstF5I 138 CATCC GGATG(2/0)
251 GGATG
CfoI 100 GCGC GCG^C
181 GCGC
EcoNI 501 CCTCCTGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
940 CCTGTGGGAGG
FokI 138 CATCC GGATG(9/13)
251 GGATG
HhaI 100 GCGC GCG^C
181 GCGC
HinP1I 100 GCGC G^CGC
181 GCGC
HinfI 418 GAGTC G^A[ACGT]TC
634 GACTC
MluNI 734 TGGCCA TGG^CCA
821 TGGCCA
MscI 734 TGGCCA TGG^CCA
821 TGGCCA
NspI 294 GCATGC [GA]CATG^[CT]
904 ACATGC
PleI 634 GACTC GAGTC(4/5)
418 GAGTC
PstI 40 CTGCAG CTGCA^G
706 CTGCAG
SfcI 40 CTGCAG C^T[GA][CT]AG
706 CTGCAG
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# 3 OR MORE CUT ENZYMES ( 51 enzymes) #
#########################################
AciI AluI AspHI AvaII BanII
BbvI BcnI BfaI BmyI BsaJI
BseRI BsiHKAI BsiYI BslI BsmFI
Bsp1286I BssKI BstNI BstXI Cac8I
Cfr13I DdeI DpnII DraII EaeI
EcoO109I EcoRII EcoT14I Fnu4HI HaeIII
HapII HpaII ItaI MaeI MboI
MboII MnlI MspI MvaI MwoI
NciI NdeII NlaIII NlaIV PpuMI
Sau3AI Sau96I ScrFI StyI TseI
TspRI