Restriction map for
pFN21AE6394
(Circular) (Six-base) MAPSORT of: pFN21AE6394.seq Check: 8958 from: 1 to: 5734
pFN21AE6394 5734 bp
With 182 enzymes: SgfI *
March 18, 2015 14:51 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2556 2556
Size: 5734
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3544 278
Size: 53 83 186 936 2008 2468
Fragments arranged by size:
2468 2008 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2451 3077 1937
Size: 514 626 4594
Fragments arranged by size:
4594 626 514
Acc65I G'GTAC_C
Cuts at: 3055 3055
Size: 5734
AclI AA'CG_TT
Cuts at: 1563 3861 4234 1563
Size: 2298 373 3063
Fragments arranged by size:
3063 2298 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2531 2822 2828 3797 5181 5449 1632
Size: 899 291 6 969 1384 268 1917
Fragments arranged by size:
1917 1384 969 899 291 268 6
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5537
AflIII A'CryG_T
Cuts at: 1203 4633 5610 1203
Size: 3430 977 1327
Fragments arranged by size:
3430 1327 977
AgeI A'CCGG_T
Cuts at: 4565 4565
Size: 5734
AhdI GACnn_n'nnGTC
Cuts at: 4463 4463
Size: 5734
AleI CACnn'nnGTG
Cuts at: 1166 2864 1166
Size: 1698 4036
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2882 1184
Size: 1698 4036
AlwNI CAG_nnn'CTG
Cuts at: 3477 5049 3477
Size: 1572 4162
ApaI G_GGCC'C
Cuts at: 2650 2650
Size: 5734
ApaLI G'TGCA_C
Cuts at: 3791 4947 3791
Size: 1156 4578
ApoI r'AATT_y
Cuts at: 1493 3043 3269 3421 5530 1493
Size: 1550 226 152 2109 1697
Fragments arranged by size:
2109 1697 1550 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4288 160
Size: 872 3256 1606
Fragments arranged by size:
3256 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5734
AvaI C'yCGr_G
Cuts at: 1870 1943 3059 1870
Size: 73 1116 4545
Fragments arranged by size:
4545 1116 73
AvrII C'CTAG_G
Cuts at: 2026 2791 2026
Size: 765 4969
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2769 2802 2769
Size: 33 5701
BamHI G'GATC_C
Cuts at: 3064 3431 3064
Size: 367 5367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3055 4510 618
Size: 325 217 627 1268 1455 1842
Fragments arranged by size:
1842 1455 1268 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2015 2650 2985 3053 1391
Size: 624 635 335 68 4072
Fragments arranged by size:
4072 635 624 335 68
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4763
BbvCI CC'TCA_GC
Cuts at: 2232 2232
Size: 5734
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3925 3959 1903
Size: 34 1988 34 3678
Fragments arranged by size:
3678 1988 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2770 3626 4836 2770
Size: 856 1210 3668
Fragments arranged by size:
3668 1210 856
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5734
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4345 136
Size: 107 122 71 631 605 2673 1525
Fragments arranged by size:
2673 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5729 5729
Size: 5734
BlpI GC'TnA_GC
Cuts at: 3151 3151
Size: 5734
Bme1580I G_kGCm'C
Cuts at: 1790 2622 2650 3795 4951 1790
Size: 832 28 1145 1156 2573
Fragments arranged by size:
2573 1156 1145 832 28
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5734
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3465 4423 442
Size: 416 53 83 399 24 2048 958 1753
Fragments arranged by size:
2048 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5734
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3016 3016
Size: 5734
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2855 2999 4394 1239
Size: 1616 144 1395 2579
Fragments arranged by size:
2579 1616 1395 144
Bpu10I CC'TnA_GC
Cuts at: 2232 2408 2232
Size: 176 5558
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2582 3859 4724 5022 2582
Size: 1277 865 298 3294
Fragments arranged by size:
3294 1277 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2659 4397 882
Size: 617 1160 1738 2219
Fragments arranged by size:
2219 1738 1160 617
BsaAI yAC'GTr
Cuts at: 493 1204 3525 493
Size: 711 2321 2702
Fragments arranged by size:
2702 2321 711
BsaBI GATnn'nnATC
Cuts at: 2697 5728 2697
Size: 3031 2703
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3541 3923 4558 275
Size: 53 83 186 936 2008 382 635 1451
Fragments arranged by size:
2008 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4167 4565 4839 4986 5397 4167
Size: 398 274 147 411 4504
Fragments arranged by size:
4504 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5509
Fragments arranged by size:
5509 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2258 2448 2696 2992 3005 1186
Size: 1072 190 248 296 13 3915
Fragments arranged by size:
3915 1072 296 248 190 13
BseYI C'CCAG_C
Cuts at: 2083 2540 4937 5433 2083
Size: 457 2397 496 2384
Fragments arranged by size:
2397 2384 496 457
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2339 1163
Size: 573 603 4558
Fragments arranged by size:
4558 603 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2103 3184 3945 4973 1540
Size: 275 186 102 1081 761 1028 2301
Fragments arranged by size:
2301 1081 1028 761 275 186 102
BsiHKAI G_wGCw'C
Cuts at: 2871 2904 2985 3795 3880 4951 2871
Size: 33 81 810 85 1071 3654
Fragments arranged by size:
3654 1071 810 85 81 33
BsmI GAATG_Cn'
Cuts at: 3245 3338 3245
Size: 93 5641
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2015 2622 2650 2871 2904 2985 3053
Size: 399 225 607 28 221 33 81 68
Cuts at: 3053 3795 3880 4951 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 607 399 225 221 85
81 68 33 28
BspEI T'CCGG_A
Cuts at: 5397 5397
Size: 5734
BspHI T'CATG_A
Cuts at: 3623 5353 3623
Size: 1730 4004
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2556 3089 844
Size: 1712 533 3489
Fragments arranged by size:
3489 1712 533
BsrBI CCG'CTC
Cuts at: 1394 1823 2268 3621 1394
Size: 429 445 1353 3507
Fragments arranged by size:
3507 1353 445 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4229 4403 65
Size: 1183 2981 174 1396
Fragments arranged by size:
2981 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2292 4378 4565 1700
Size: 110 482 2086 187 2869
Fragments arranged by size:
2869 2086 482 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5734
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5734
BssSI C'ACGA_G
Cuts at: 3794 4806 3794
Size: 1012 4722
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2072 794
Size: 1278 4456
BstBI TT'CG_AA
Cuts at: 4538 4538
Size: 5734
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5734
BstXI CCAn_nnnn'nTGG
Cuts at: 2349 2578 2676 2349
Size: 229 98 5407
Fragments arranged by size:
5407 229 98
BstYI r'GATC_y
Cuts at: 1972 2760 3064 3431 3816 3833 5285 5371 5383
Size: 788 304 367 385 17 1452 86 12
Cuts at: 5383 5729 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 788 385 367 346 304 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4243
Fragments arranged by size:
4243 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4995
Fragments arranged by size:
4995 683 56
BtsI GCAGTG_nn'
Cuts at: 2066 2192 3250 4044 4064 2066
Size: 126 1058 794 20 3736
Fragments arranged by size:
3736 1058 794 126 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5734
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5699
DraI TTT'AAA
Cuts at: 3038 3038
Size: 5734
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4741 809
Size: 507 3425 1802
Fragments arranged by size:
3425 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2259 2739 2826
Size: 54 1425 50 275 18 429 480 87
Cuts at: 2826 3181 4070 5699 8
Size: 355 889 1629 43
Fragments arranged by size:
1629 1425 889 480 429 355 275 87
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3181 1537
Size: 275 1369 4090
Fragments arranged by size:
4090 1369 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2216 3664 1353
Size: 863 1448 3423
Fragments arranged by size:
3423 1448 863
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4321 4695 4841 387
Size: 1064 138 2732 374 146 1280
Fragments arranged by size:
2732 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2983 2983
Size: 5734
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2531 2822 2828 2855 2999 3797 4394
Size: 393 899 291 6 27 144 798 597
Cuts at: 4394 5181 5449 1239
Size: 787 268 1524
Fragments arranged by size:
1524 899 798 787 597 393 291 268
144 27 6
EcoNI CCTnn'n_nnAGG
Cuts at: 2510 2949 2510
Size: 439 5295
EcoO109I rG'GnC_Cy
Cuts at: 2164 2440 2503 2524 2646 2955 2995 2164
Size: 276 63 21 122 309 40 4903
Fragments arranged by size:
4903 309 276 122 63 40 21
EcoRI G'AATT_C
Cuts at: 3043 3421 3043
Size: 378 5356
FspI TGC'GCA
Cuts at: 4240 4240
Size: 5734
HaeII r_GCGC'y
Cuts at: 1678 4881 1678
Size: 3203 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2987 3019 4375 4407 4449
Size: 32 135 32 1415 32 1356 32 42
Cuts at: 4449 4481 1373
Size: 32 2626
Fragments arranged by size:
2626 1415 1356 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3078 3324 669
Size: 988 281 1140 246 3079
Fragments arranged by size:
3079 1140 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5734
HpaI GTT'AAC
Cuts at: 3324 3324
Size: 5734
KpnI G_GTAC'C
Cuts at: 3059 3059
Size: 5734
MfeI C'AATT_G
Cuts at: 3333 3333
Size: 5734
MluI A'CGCG_T
Cuts at: 5610 5610
Size: 5734
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4848 5032 1305
Size: 3543 184 2007
Fragments arranged by size:
3543 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2741 2828 10
Size: 54 1425 343 909 87 2916
Fragments arranged by size:
2916 1425 909 343 87 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2864 3692 4051 4210 518
Size: 648 1698 828 359 159 2042
Fragments arranged by size:
2042 1698 828 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2783 3150 3827 4975 5220 1606
Size: 1177 367 677 1148 245 2120
Fragments arranged by size:
2120 1177 1148 677 367 245
NaeI GCC'GGC
Cuts at: 2294 2294
Size: 5734
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4243
Fragments arranged by size:
4243 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5734
NgoMIV G'CCGG_C
Cuts at: 2292 2292
Size: 5734
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5734
NotI GC'GGCC_GC
Cuts at: 3181 3181
Size: 5734
NspI r_CATG'y
Cuts at: 1132 1229 2303 2913 3092 4637 1132
Size: 97 1074 610 179 1545 2229
Fragments arranged by size:
2229 1545 1074 610 179 97
PasI CC'CwG_GG
Cuts at: 1348 2015 2443 2528 1348
Size: 667 428 85 4554
Fragments arranged by size:
4554 667 428 85
PciI A'CATG_T
Cuts at: 4633 4633
Size: 5734
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5734
PfoI T'CCnGG_A
Cuts at: 2310 2871 2889 2310
Size: 561 18 5155
Fragments arranged by size:
5155 561 18
PmeI GTTT'AAAC
Cuts at: 3038 3038
Size: 5734
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3803 3835 5316 5348 3803
Size: 32 1481 32 4189
Fragments arranged by size:
4189 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2524 2955 2995 2524
Size: 431 40 5263
Fragments arranged by size:
5263 431 40
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5734
PsiI TTA'TAA
Cuts at: 3304 3304
Size: 5734
PspOMI G'GGCC_C
Cuts at: 2646 2646
Size: 5734
PstI C_TGCA'G
Cuts at: 830 2049 2715 3086 830
Size: 1219 666 371 3478
Fragments arranged by size:
3478 1219 666 371
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5734
SacI G_AGCT'C
Cuts at: 2985 2985
Size: 5734
SalI G'TCGA_C
Cuts at: 1936 3076 1936
Size: 1140 4594
SanDI GG'GwC_CC
Cuts at: 2524 2955 2524
Size: 431 5303
SbfI CC_TGCA'GG
Cuts at: 3086 3086
Size: 5734
ScaI AGT'ACT
Cuts at: 3982 5694 3982
Size: 1712 4022
SfcI C'TryA_G
Cuts at: 826 1045 2045 2711 3082 4217 4898 5089 826
Size: 219 1000 666 371 1135 681 191 1471
Fragments arranged by size:
1471 1135 1000 681 666 371 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5734
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5734
SmaI CCC'GGG
Cuts at: 1872 3061 1872
Size: 1189 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2597 3838 4739 5001 820
Size: 197 926 654 1241 901 262 1553
Fragments arranged by size:
1553 1241 926 901 654 262 197
SnaBI TAC'GTA
Cuts at: 493 3525 493
Size: 3032 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5734
SphI G_CATG'C
Cuts at: 1132 2303 3092 1132
Size: 1171 789 3774
Fragments arranged by size:
3774 1171 789
SspI AAT'ATT
Cuts at: 5 52 3658 5
Size: 47 3606 2081
Fragments arranged by size:
3606 2081 47
StuI AGG'CCT
Cuts at: 1517 2516 1517
Size: 999 4735
StyI C'CwwG_G
Cuts at: 513 1065 2004 2026 2127 2791 3173 513
Size: 552 939 22 101 664 382 3074
Fragments arranged by size:
3074 939 664 552 382 101 22
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2435 2682 2756 3761 3778 3931 4116
Size: 1255 855 247 74 1005 17 153 185
Cuts at: 4116 4561 325
Size: 445 1498
Fragments arranged by size:
1498 1255 1005 855 445 247 185 153
74 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3980 5692 96
Size: 274 80 33 51 1443 2003 1712 138
Fragments arranged by size:
2003 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5694 5726 5694
Size: 32 5702
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5734
XbaI T'CTAG_A
Cuts at: 3070 3070
Size: 5734
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2483 2483
Size: 5734
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5734
XmaI C'CCGG_G
Cuts at: 1870 3059 1870
Size: 1189 4545
XmnI GAAnn'nnTTC
Cuts at: 3863 3863
Size: 5734
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3542 276
Size: 53 83 186 936 2008 2468
Fragments arranged by size:
2468 2008 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI AvrII BaeI BamHI BanI BanII
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI
PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI
PspOMI PstI PvuI SacI SalI SanDI SbfI ScaI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI BbeI BfrBI BsiWI BsmBI BstZ17I
Bsu36I DraIII EcoRV FalI FseI FspAI KasI NarI
NruI NsiI PacI PmlI PspXI PsrI PvuII RsrII
SacII SapI SexAI SfiI SfoI SrfI SwaI