################################## # NO CUT ENZYMES ( 132 enzymes) # ################################## AatII Acc65I AccIII AclI AflII AflIII AgeI AhdI Alw44I AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp718I AspEI AviII AvrII BamHI BbeI BbrPI BbvCI BcgI BciVI BclI BfrI BglI BlnI BlpI Bpu1102I BsaAI BsaBI BsaWI BseAI BsiEI BsiWI BsmBI Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrFI BsrGI BssHII BssSI Bst1107I BstBI BstEII BstPI BstXI BstZ17I Bsu36I CelII Cfr10I ClaI CpoI DraI DraIII DrdI EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I EcoRV FbaI FseI FspI HincII HindII HindIII HpaI KasI KpnI Ksp632I KspI MamI MluI MroI NaeI NarI NcoI NgoMIV NheI NotI NruI NspV PacI PflMI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RcaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI SwaI TfiI Van91I XbaI XcmI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 37 enzymes) # ################################### AccI 1132 GTAGAC GT^[AC][GT]AC AccII 1289 CGCG CG^CG AcyI 1135 GACGCC G[GA]^CG[CT]C AlwNI 616 CAGGCTCTG CAG[ACGT][ACGT][ACGT]^CTG Asp700I 540 GAAAGATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspHI 64 GTGCTC G[AT]GC[AT]^C BanI 1653 GGCACC G^G[CT][GA]CC BbsI 289 GAAGAC GAAGAC(2/6) BpuAI 289 GAAGAC GAAGAC(2/6) BsaHI 1135 GACGCC G[GA]^CG[CT]C BsaI 500 GGTCTC GGTCTC(1/5) BsiHKAI 64 GTGCTC G[AT]GC[AT]^C BsmI 1242 GAATGC GAATGC(1/-1) BsrBI 13 GAGCGG CCGCTC(-3/-3) BsrDI 435 CATTGC GCAATG(2/0) BstAPI 1237 GCAAAGAATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstUI 1289 CGCG CG^CG DsaI 5 CCACGG C^C[GA][CT]GG EcoRI 1276 GAATTC G^AATTC EcoT14I 1678 CCAAGG C^C[AT][AT]GG EcoT22I 358 ATGCAT ATGCA^T Hin1I 1135 GACGCC G[GA]^CG[CT]C MaeII 752 ACGT A^CGT MaeIII 793 GTCAC ^GT[ACGT]AC MfeI 518 CAATTG C^AATTG MunI 518 CAATTG C^AATTG MvnI 1289 CGCG CG^CG NdeI 690 CATATG CA^TATG NsiI 358 ATGCAT ATGCA^T PaeR7I 503 CTCGAG C^TCGAG PstI 768 CTGCAG CTGCA^G SfcI 768 CTGCAG C^T[GA][CT]AG SmlI 503 CTCGAG C^T[CT][GA]AG StyI 1678 CCAAGG C^C[AT][AT]GG Tsp45I 793 GTCAC ^GT[GC]AC XhoI 503 CTCGAG C^TCGAG XmnI 540 GAAAGATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 16 enzymes) # ################################### AlwI 984 GATCC GGATC(4/5) 585 GGATC AspI 757 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC 787 GACTCGGTC AvaI 260 CCCGAG C^[CT]CG[GA]G 503 CTCGAG BanII 20 GAGCCC G[GA]GC[CT]^C 1203 GGGCTC BglII 465 AGATCT A^GATCT 507 AGATCT BsoBI 260 CCCGAG C^[CT]CG[GA]G 503 CTCGAG BstYI 465 AGATCT [GA]^GATC[CT] 507 AGATCT HphI 935 TCACC GGTGA(8/7) 1451 TCACC MflI 465 AGATCT [GA]^GATC[CT] 507 AGATCT MspA1I 559 CAGCTG C[AC]G^C[GT]G 674 CAGCTG NspI 356 ACATGC [GA]CATG^[CT] 741 ACATGC PflFI 757 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC 787 GACTCGGTC PvuII 559 CAGCTG CAG^CTG 674 CAGCTG StuI 312 AGGCCT AGG^CCT 1681 AGGCCT Tth111I 757 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC 787 GACTCGGTC XhoII 465 AGATCT [GA]^GATC[CT] 507 AGATCT ######################################### # 3 OR MORE CUT ENZYMES ( 73 enzymes) # ######################################### AciI AcsI AfaI AluI ApoI AvaII BalI BbvI BcnI BfaI BmyI BpmI BsaJI BseRI BsgI BsiYI BslI BsmAI BsmFI Bsp1286I BsrI BssKI BstF5I BstNI Cac8I CfoI Cfr13I DdeI DpnII DraII EaeI EcoO109I EcoRII FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI HinP1I HinfI HpaII ItaI MaeI MboI MboII MluNI MnlI MscI MseI MslI MspI MvaI MwoI NciI NdeII NlaIII NlaIV PleI PpuMI RsaI Sau3AI Sau96I ScrFI SfaNI TaqI Tru9I TseI Tsp509I TspRI TthHB8I