################################## # NO CUT ENZYMES ( 184 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AciI AclI AcyI AflII AgeI AhdI AlwI AlwNI AocI Aor51HI ApaI AscI AseI AsnI Asp700I Asp718I AspEI AspI AvaI AviII AvrII BamHI BanI BanII BbeI BbrPI BcgI BciVI BclI BcnI BfrI BglI BglII BlnI BlpI BpmI Bpu1102I BsaAI BsaBI BsaHI BsaI BsaWI BseAI BsgI BsiEI BsiWI BsmBI BsmI BsoBI Bsp1407I BspDI BspEI BspHI BspMI BsrBI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstXI BstYI BstZ17I Bsu36I CelII CfoI Cfr10I ClaI CpoI DpnII DraI DrdI DsaI EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I EcoRV FauI FbaI FseI FspI HaeII HapII HgaI HhaI Hin1I HinP1I HpaI HpaII HphI KasI KpnI Ksp632I KspI MaeIII MamI MboI MflI MluI MroI MseI MslI MspA1I MspI MvnI MwoI NaeI NarI NciI NcoI NdeI NdeII NgoMIV NheI NotI NruI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI PvuII RcaI RsrII SacI SacII SalI SapI Sau3AI SbfI ScaI SexAI SfcI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI Tru9I Tsp45I Tth111I Van91I XbaI XcmI XhoI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 37 enzymes) # ################################### AcsI 222 GAATTC [GA]^AATT[CT] AflIII 461 ACATGT A^C[GA][CT]GT Alw44I 289 GTGCAC G^TGCAC ApaLI 289 GTGCAC G^TGCAC ApoI 222 GAATTC [GA]^AATT[CT] AspHI 289 GTGCAC G[AT]GC[AT]^C BalI 185 TGGCCA TGG^CCA BbvCI 490 GCTGAGG CCTCAGC(-5/-2) BmyI 289 GTGCAC G[AGT]GC[ACT]^C BsaJI 389 CCAAGG C^C[ACGT][ACGT]GG BsiHKAI 289 GTGCAC G[AT]GC[AT]^C BsiYI 385 CCTCCCAAGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BslI 385 CCTCCCAAGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BsmAI 581 GAGAC GTCTC(1/5) Bsp1286I 289 GTGCAC G[AGT]GC[ACT]^C BspLU11I 461 ACATGT A^CATGT BsrDI 453 CATTGC GCAATG(2/0) BstF5I 208 GGATG GGATG(2/0) DraII 392 AGGGCCT [GA]G^G[ACGT]CC[CT] DraIII 178 CACAATGTG CAC[ACGT][ACGT][ACGT]^GTG EaeI 185 TGGCCA [CT]^GGCC[GA] EcoO109I 392 AGGGCCT [GA]G^G[ACGT]CC[CT] EcoRI 222 GAATTC G^AATTC EcoT14I 389 CCAAGG C^C[AT][AT]GG EcoT22I 282 ATGCAT ATGCA^T FokI 208 GGATG GGATG(9/13) HindIII 355 AAGCTT A^AGCTT MfeI 53 CAATTG C^AATTG MluNI 185 TGGCCA TGG^CCA MscI 185 TGGCCA TGG^CCA MunI 53 CAATTG C^AATTG NlaIV 369 GGGACC GG[ACGT]^[ACGT]CC NsiI 282 ATGCAT ATGCA^T StyI 389 CCAAGG C^C[AT][AT]GG TaqI 425 TCGA T^CGA TfiI 474 GATTC G^A[AT]TC TthHB8I 425 TCGA T^CGA ################################### # TWO CUT ENZYMES ( 26 enzymes) # ################################### AfaI 402 GTAC GT^AC 550 GTAC AvaII 370 GGACC G^G[AT]CC 379 GGACC BbsI 34 GAAGAC GAAGAC(2/6) 276 GAAGAC BbvI 150 GCAGC GCAGC(8/12) 253 GCTGC BfaI 85 CTAG C^TAG 97 CTAG BpuAI 34 GAAGAC GAAGAC(2/6) 276 GAAGAC BseRI 12 CTCCTC GAGGAG(10/8) 493 GAGGAG BsmFI 212 GGGAC GGGAC(10/14) 369 GGGAC BsrI 339 ACTGG ACTGG(1/-1) 545 CCAGT BssKI 218 CCTGG ^CC[ACGT]GG 373 CCAGG BstNI 218 CCTGG CC^[AT]GG 373 CCAGG Cac8I 3 GCTTGC GC[ACGT]^[ACGT]GC 508 GCAAGC EcoRII 218 CCTGG ^CC[AT]GG 373 CCAGG Fnu4HI 150 GCAGC GC^[ACGT]GC 253 GCTGC HaeIII 186 GGCC GG^CC 394 GGCC ItaI 150 GCAGC GC^[ACGT]GC 253 GCTGC MaeI 85 CTAG C^TAG 97 CTAG MaeII 116 ACGT A^CGT 532 ACGT MboII 34 GAAGA GAAGA(8/7) 276 GAAGA MvaI 218 CCTGG CC^[AT]GG 373 CCAGG NspI 280 ACATGC [GA]CATG^[CT] 461 ACATGT RsaI 402 GTAC GT^AC 550 GTAC ScrFI 218 CCTGG CC^[ACGT]GG 373 CCAGG SfaNI 334 GATGC GCATC(5/9) 498 GATGC TseI 150 GCAGC G^C[AT]GC 253 GCTGC Tsp509I 54 AATT ^AATT 223 AATT ######################################### # 3 OR MORE CUT ENZYMES ( 11 enzymes) # ######################################### AluI Cfr13I DdeI HincII HindII HinfI MnlI NlaIII PleI Sau96I TspRI