################################## # NO CUT ENZYMES ( 137 enzymes) # ################################## AatII Acc65I AccII AccIII AclI AgeI AhdI Alw44I AlwNI AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AvaI AviII AvrII BalI BamHI BanI BbeI BbrPI BbsI BclI BcnI BglI BlnI BlpI Bpu1102I BpuAI BsaAI BsaBI BsaI BseAI BsgI BsiEI BsiHKAI BsiWI BsoBI Bsp1407I BspDI BspEI BspMI BsrDI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstXI BstZ17I Bsu36I CelII ClaI CpoI DraIII EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I EcoRV EcoT22I FbaI FseI FspI HaeII HpaI KasI KpnI KspI MamI MfeI MluI MluNI MroI MscI MunI MvnI NaeI NarI NciI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PstI PvuI RsrII SacI SacII SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI Sse8387I SspBI SspI SwaI XbaI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 52 enzymes) # ################################### AcyI 1213 GGCGTC G[GA]^CG[CT]C AfaI 249 GTAC GT^AC AflII 187 CTTAAG C^TTAAG AflIII 251 ACATGT A^C[GA][CT]GT AspI 139 GACGGTGTC GAC[ACGT]^[ACGT][ACGT]GTC BbvCI 691 GCTGAGG CCTCAGC(-5/-2) BcgI 453 GCAAAGGGATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BciVI 1036 GGATAC GTATCC(6/5) BfrI 187 CTTAAG C^TTAAG BglII 333 AGATCT A^GATCT BsaHI 1213 GGCGTC G[GA]^CG[CT]C BsaWI 485 ACCGGA [AT]^CCGG[AT] BsmBI 1251 CGTCTC CGTCTC(1/5) BsmI 1051 GAATGC GAATGC(1/-1) BspHI 71 TCATGA T^CATGA BspLU11I 251 ACATGT A^CATGT BsrBI 979 CCGCTC CCGCTC(-3/-3) BsrFI 348 GCCGGT [GA]^CCGG[CT] BstYI 333 AGATCT [GA]^GATC[CT] CfoI 26 GCGC GCG^C Cfr10I 348 GCCGGT [GA]^CCGG[CT] DraI 1231 TTTAAA TTT^AAA DraII 973 AGGACCC [GA]G^G[ACGT]CC[CT] DrdI 1207 GACAAAGGCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC DsaI 1056 CCGTGG C^C[GA][CT]GG EaeI 1247 TGGCCG [CT]^GGCC[GA] EcoO109I 973 AGGACCC [GA]G^G[ACGT]CC[CT] EcoRI 1137 GAATTC G^AATTC FauI 978 CCCGC CCCGC(4/6) HgaI 1214 GCGTC GACGC(5/10) HhaI 26 GCGC GCG^C Hin1I 1213 GGCGTC G[GA]^CG[CT]C HinP1I 26 GCGC G^CGC HincII 145 GTCGAC GT[CT]^[GA]AC HindII 145 GTCGAC GT[CT]^[GA]AC HindIII 1148 AAGCTT A^AGCTT MaeII 624 ACGT A^CGT MflI 333 AGATCT [GA]^GATC[CT] MslI 444 CACACTGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MspA1I 655 CAGCTG C[AC]G^C[GT]G PflFI 139 GACGGTGTC GAC[ACGT]^[ACGT][ACGT]GTC PpuMI 973 AGGACCC [GA]G^G[AT]CC[CT] PvuII 655 CAGCTG CAG^CTG RcaI 71 TCATGA T^CATGA RsaI 249 GTAC GT^AC SalI 145 GTCGAC G^TCGAC SfcI 1266 CTGTAG C^T[GA][CT]AG SphI 1104 GCATGC GCATG^C StuI 1118 AGGCCT AGG^CCT Tth111I 139 GACGGTGTC GAC[ACGT]^[ACGT][ACGT]GTC XcmI 647 CCACCCACCAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoII 333 AGATCT [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 23 enzymes) # ################################### AccI 145 GTCGAC GT^[AC][GT]AC 481 GTAGAC AciI 979 CCGC CCGC(-3/-1) 1030 CCGC AcsI 271 AAATTT [GA]^AATT[CT] 1137 GAATTC AlwI 459 GGATC GGATC(4/5) 1019 GGATC ApoI 271 AAATTT [GA]^AATT[CT] 1137 GAATTC BbvI 43 GCAGC GCAGC(8/12) 62 GCAGC BseRI 89 CTCCTC GAGGAG(10/8) 382 CTCCTC BsmFI 1010 GGGAC GGGAC(10/14) 108 GTCCC EcoT14I 230 CCAAGG C^C[AT][AT]GG 1121 CCTTGG Fnu4HI 43 GCAGC GC^[ACGT]GC 62 GCAGC HapII 349 CCGG C^CGG 486 CCGG HpaII 349 CCGG C^CGG 486 CCGG ItaI 43 GCAGC GC^[ACGT]GC 62 GCAGC MspI 349 CCGG C^CGG 486 CCGG PflMI 651 CCACCAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1116 CCAGGCCTTGG PleI 925 GACTC GAGTC(4/5) 1012 GACTC SmlI 187 CTTAAG C^T[CT][GA]AG 1132 CTCAAG StyI 230 CCAAGG C^C[AT][AT]GG 1121 CCTTGG TaqI 146 TCGA T^CGA 989 TCGA TfiI 223 GATTC G^A[AT]TC 938 GAATC TseI 43 GCAGC G^C[AT]GC 62 GCAGC TthHB8I 146 TCGA T^CGA 989 TCGA Van91I 651 CCACCAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1116 CCAGGCCTTGG ######################################### # 3 OR MORE CUT ENZYMES ( 46 enzymes) # ######################################### AluI AvaII BanII BfaI BmyI BpmI BsaJI BsiYI BslI BsmAI Bsp1286I BsrI BssKI BstF5I BstNI Cac8I Cfr13I DdeI DpnII EarI EcoRII FokI HaeIII HinfI HphI Ksp632I MaeI MaeIII MboI MboII MnlI MseI MvaI MwoI NdeII NlaIII NlaIV NspI Sau3AI Sau96I ScrFI SfaNI Tru9I Tsp45I Tsp509I TspRI