(Circular) (Six-base) MAPSORT of: pFN21AE2298.seq Check: 6779 from: 1 to: 5977 pFN21AE2298 5977 bp With 182 enzymes: SgfI * November 10, 2010 12:23 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3787 278 Size: 53 83 186 936 2251 2468 Fragments arranged by size: 2468 2251 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2151 2487 3320 1937 Size: 214 336 833 4594 Fragments arranged by size: 4594 833 336 214 Acc65I G'GTAC_C Cuts at: 3298 3298 Size: 5977 AclI AA'CG_TT Cuts at: 1563 4104 4477 1563 Size: 2541 373 3063 Fragments arranged by size: 3063 2541 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 4040 5424 5692 1632 Size: 2408 1384 268 1917 Fragments arranged by size: 2408 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 2192 820 Size: 197 1175 4605 Fragments arranged by size: 4605 1175 197 AflIII A'CryG_T Cuts at: 1203 2256 4876 5853 1203 Size: 1053 2620 977 1327 Fragments arranged by size: 2620 1327 1053 977 AgeI A'CCGG_T Cuts at: 4808 4808 Size: 5977 AhdI GACnn_n'nnGTC Cuts at: 4706 4706 Size: 5977 AleI CACnn'nnGTG Cuts at: 1166 2453 1166 Size: 1287 4690 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5977 AlwNI CAG_nnn'CTG Cuts at: 3720 5292 3720 Size: 1572 4405 ApaLI G'TGCA_C Cuts at: 4034 5190 4034 Size: 1156 4821 ApoI r'AATT_y Cuts at: 1493 2276 3142 3286 3512 3664 5773 1493 Size: 783 866 144 226 152 2109 1697 Fragments arranged by size: 2109 1697 866 783 226 152 144 AseI AT'TA_AT Cuts at: 160 1032 4531 160 Size: 872 3499 1606 Fragments arranged by size: 3499 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5977 AvaI C'yCGr_G Cuts at: 1870 1943 3302 1870 Size: 73 1359 4545 Fragments arranged by size: 4545 1359 73 BamHI G'GATC_C Cuts at: 3307 3674 3307 Size: 367 5610 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3298 4753 618 Size: 325 217 627 1511 1455 1842 Fragments arranged by size: 1842 1511 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2012 2373 3192 3296 1391 Size: 621 361 819 104 4072 Fragments arranged by size: 4072 819 621 361 104 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5006 BbvCI CC'TCA_GC Cuts at: 2697 2697 Size: 5977 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2447 2481 4168 4202 1903 Size: 34 510 34 1687 34 3678 Fragments arranged by size: 3678 1687 510 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3035 3869 5079 3035 Size: 834 1210 3933 Fragments arranged by size: 3933 1210 834 BclI T'GATC_A Cuts at: 1548 1548 Size: 5977 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4588 136 Size: 107 122 71 631 605 2916 1525 Fragments arranged by size: 2916 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2338 5972 2338 Size: 3634 2343 BlpI GC'TnA_GC Cuts at: 3394 3394 Size: 5977 Bme1580I G_kGCm'C Cuts at: 1790 2740 3177 4038 5194 1790 Size: 950 437 861 1156 2573 Fragments arranged by size: 2573 1156 950 861 437 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5977 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3708 4666 442 Size: 416 53 83 399 24 2291 958 1753 Fragments arranged by size: 2291 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5977 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3211 3211 Size: 5977 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2153 2537 2684 4637 1239 Size: 914 384 147 1953 2579 Fragments arranged by size: 2579 1953 914 384 147 Bpu10I CC'TnA_GC Cuts at: 2697 2697 Size: 5977 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3122 4102 4967 5265 3122 Size: 980 865 298 3834 Fragments arranged by size: 3834 980 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4640 882 Size: 617 3141 2219 Fragments arranged by size: 3141 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3768 493 Size: 711 2564 2702 Fragments arranged by size: 2702 2564 711 BsaBI GATnn'nnATC Cuts at: 5971 5971 Size: 5977 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3219 3784 4166 4801 Size: 53 83 186 936 1686 565 382 635 Cuts at: 4801 275 Size: 1451 Fragments arranged by size: 1686 1451 936 635 565 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2490 4410 4808 5082 5229 5640 2490 Size: 1920 398 274 147 411 2827 Fragments arranged by size: 2827 1920 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2996 3026 1178 Size: 30 165 30 1593 30 4129 Fragments arranged by size: 4129 1593 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2085 2378 1186 Size: 899 293 4785 Fragments arranged by size: 4785 899 293 BseYI C'CCAG_C Cuts at: 2349 5180 5676 2349 Size: 2831 496 2650 Fragments arranged by size: 2831 2650 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5404 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3427 4188 5216 1540 Size: 275 186 1426 761 1028 2301 Fragments arranged by size: 2301 1426 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 4038 4123 5194 4038 Size: 85 1071 4821 Fragments arranged by size: 4821 1071 85 BsmI GAATG_Cn' Cuts at: 3062 3488 3581 3062 Size: 426 93 5458 Fragments arranged by size: 5458 426 93 BsmBI CGTCTCn'nnnn_ Cuts at: 3262 3262 Size: 5977 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2012 2373 2740 3177 3192 3296 4038 Size: 399 222 361 367 437 15 104 742 Cuts at: 4038 4123 5194 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 437 399 367 361 222 104 85 15 BspEI T'CCGG_A Cuts at: 5640 5640 Size: 5977 BspHI T'CATG_A Cuts at: 2076 3866 5596 2076 Size: 1790 1730 2457 Fragments arranged by size: 2457 1790 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3332 844 Size: 2488 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2986 3864 1394 Size: 429 1163 878 3507 Fragments arranged by size: 3507 1163 878 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4472 4646 65 Size: 1183 3224 174 1396 Fragments arranged by size: 3224 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2353 4621 4808 1700 Size: 110 543 2268 187 2869 Fragments arranged by size: 2869 2268 543 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5977 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5977 BssSI C'ACGA_G Cuts at: 4037 5049 4037 Size: 1012 4965 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5977 BstBI TT'CG_AA Cuts at: 4781 4781 Size: 5977 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5977 BstYI r'GATC_y Cuts at: 1972 2338 3307 3674 4059 4076 5528 5614 5626 Size: 366 969 367 385 17 1452 86 12 Cuts at: 5626 5972 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 969 385 367 366 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 3061 513 Size: 552 939 1057 3429 Fragments arranged by size: 3429 1057 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3013 487 Size: 683 56 1787 3451 Fragments arranged by size: 3451 1787 683 56 BtsI GCAGTG_nn' Cuts at: 2162 2716 2794 2934 3493 4287 4307 2162 Size: 554 78 140 559 794 20 3832 Fragments arranged by size: 3832 794 559 554 140 78 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5977 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5942 DraI TTT'AAA Cuts at: 3238 3281 3238 Size: 43 5934 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3218 4984 809 Size: 507 1902 1766 1802 Fragments arranged by size: 1902 1802 1766 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3252 3424 4313 Size: 54 1425 50 275 18 1422 172 889 Cuts at: 4313 5942 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1422 889 275 172 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3424 1537 Size: 275 1612 4090 Fragments arranged by size: 4090 1612 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2396 2412 2886 3907 1353 Size: 1043 16 474 1021 3423 Fragments arranged by size: 3423 1043 1021 474 16 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3024 4564 4938 5084 387 Size: 1064 138 1435 1540 374 146 1280 Fragments arranged by size: 1540 1435 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2153 2537 2684 4040 4637 5424 5692 Size: 393 521 384 147 1356 597 787 268 Cuts at: 5692 1239 Size: 1524 Fragments arranged by size: 1524 1356 787 597 521 393 384 268 147 EcoO109I rG'GnC_Cy Cuts at: 2979 2979 Size: 5977 EcoRI G'AATT_C Cuts at: 3142 3286 3664 3142 Size: 144 378 5455 Fragments arranged by size: 5455 378 144 FspI TGC'GCA Cuts at: 4483 4483 Size: 5977 HaeII r_GCGC'y Cuts at: 1678 5124 1678 Size: 3446 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2117 2149 2279 2311 2904 Size: 32 135 32 545 32 130 32 593 Cuts at: 2904 2936 3016 3048 4618 4650 4692 4724 1373 Size: 32 80 32 1570 32 42 32 2626 Fragments arranged by size: 2626 1570 593 545 135 130 80 42 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2152 3321 3567 669 Size: 988 281 214 1169 246 3079 Fragments arranged by size: 3079 1169 988 281 246 214 HindIII A'AGCT_T Cuts at: 748 3153 748 Size: 2405 3572 HpaI GTT'AAC Cuts at: 3567 3567 Size: 5977 KpnI G_GTAC'C Cuts at: 3302 3302 Size: 5977 MfeI C'AATT_G Cuts at: 3576 3576 Size: 5977 MluI A'CGCG_T Cuts at: 5853 5853 Size: 5977 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2241 5091 5275 1305 Size: 936 2850 184 2007 Fragments arranged by size: 2850 2007 936 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4155 Fragments arranged by size: 4155 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2453 3935 4294 4453 518 Size: 648 1287 1482 359 159 2042 Fragments arranged by size: 2042 1482 1287 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2662 3393 4070 5218 5463 1606 Size: 1056 731 677 1148 245 2120 Fragments arranged by size: 2120 1148 1056 731 677 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4486 Fragments arranged by size: 4486 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5977 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5977 NotI GC'GGCC_GC Cuts at: 3424 3424 Size: 5977 NspI r_CATG'y Cuts at: 1132 1229 2260 2554 3113 3335 4880 1132 Size: 97 1031 294 559 222 1545 2229 Fragments arranged by size: 2229 1545 1031 559 294 222 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5977 PciI A'CATG_T Cuts at: 2256 4876 2256 Size: 2620 3357 PflMI CCAn_nnn'nTGG Cuts at: 1108 2662 3127 1108 Size: 1554 465 3958 Fragments arranged by size: 3958 1554 465 PfoI T'CCnGG_A Cuts at: 3020 3020 Size: 5977 PmeI GTTT'AAAC Cuts at: 3281 3281 Size: 5977 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4046 4078 5559 5591 4046 Size: 32 1481 32 4432 Fragments arranged by size: 4432 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2979 2979 Size: 5977 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5977 PsiI TTA'TAA Cuts at: 3547 3547 Size: 5977 PstI C_TGCA'G Cuts at: 830 3329 830 Size: 2499 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5977 PvuII CAG'CTG Cuts at: 2662 2662 Size: 5977 SalI G'TCGA_C Cuts at: 1936 2150 3319 1936 Size: 214 1169 4594 Fragments arranged by size: 4594 1169 214 SbfI CC_TGCA'GG Cuts at: 3329 3329 Size: 5977 ScaI AGT'ACT Cuts at: 4225 5937 4225 Size: 1712 4265 SfcI C'TryA_G Cuts at: 826 1045 3271 3325 4460 5141 5332 826 Size: 219 2226 54 1135 681 191 1471 Fragments arranged by size: 2226 1471 1135 681 219 191 54 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5977 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5977 SmaI CCC'GGG Cuts at: 1872 3304 1872 Size: 1432 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2192 3137 4081 4982 5244 820 Size: 197 926 249 945 944 901 262 1553 Fragments arranged by size: 1553 945 944 926 901 262 249 197 SnaBI TAC'GTA Cuts at: 493 3768 493 Size: 3275 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5977 SphI G_CATG'C Cuts at: 1132 3113 3335 1132 Size: 1981 222 3774 Fragments arranged by size: 3774 1981 222 SspI AAT'ATT Cuts at: 5 52 3901 5 Size: 47 3849 2081 Fragments arranged by size: 3849 2081 47 StuI AGG'CCT Cuts at: 1517 3125 1517 Size: 1608 4369 StyI C'CwwG_G Cuts at: 513 1065 2004 2235 3126 3416 513 Size: 552 939 231 891 290 3074 Fragments arranged by size: 3074 939 891 552 290 231 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2669 3000 3115 4004 4021 4174 4359 Size: 1255 1089 331 115 889 17 153 185 Cuts at: 4359 4804 325 Size: 445 1498 Fragments arranged by size: 1498 1255 1089 889 445 331 185 153 115 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2253 4223 5935 Size: 274 80 33 51 1443 276 1970 1712 Cuts at: 5935 96 Size: 138 Fragments arranged by size: 1970 1712 1443 276 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2996 3028 5937 5969 2996 Size: 32 2909 32 3004 Fragments arranged by size: 3004 2909 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2147 1316 Size: 831 5146 XbaI T'CTAG_A Cuts at: 3313 3313 Size: 5977 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2011 2659 2011 Size: 648 5329 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5977 XmaI C'CCGG_G Cuts at: 1870 3302 1870 Size: 1432 4545 XmnI GAAnn'nnTTC Cuts at: 4106 4106 Size: 5977 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3785 276 Size: 53 83 186 936 2251 2468 Fragments arranged by size: 2468 2251 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BfrBI BsiWI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI