################################## # NO CUT ENZYMES ( 164 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AciI AclI AcyI AflII AflIII AgeI AhdI Alw44I AlwNI AocI Aor51HI ApaI ApaLI AscI Asp718I AspEI AspHI AvaI AvaII AviII AvrII BamHI BanI BbeI BbvCI BcgI BclI BcnI BfaI BfrI BglI BglII BlnI BlpI Bpu1102I BsaBI BsaHI BsaWI BseAI BseRI BsiEI BsiHKAI BsiWI BsmBI BsmI BsoBI BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrDI BsrFI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstZ17I Bsu36I CelII Cfr10I Cfr13I ClaI CpoI DraI DraII DraIII DrdI EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRI EcoT22I FauI FbaI FseI FspI HapII HgaI Hin1I HindIII HpaI HpaII KasI KpnI Ksp632I KspI MaeI MamI MfeI MluI MroI MspI MunI MvnI NaeI NarI NciI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflMI PinAI PmeI PpuMI PshAI Psp1406I PstI PvuI RcaI RsrII SacI SacII SalI SapI Sau96I SbfI ScaI SexAI SfaNI SfcI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspI StuI SwaI TfiI Van91I XbaI XcmI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 37 enzymes) # ################################### AfaI 63 GTAC GT^AC AseI 913 ATTAAT AT^TAAT AsnI 913 ATTAAT AT^TAAT BalI 248 TGGCCA TGG^CCA BanII 261 GGGCTC G[GA]GC[CT]^C BbrPI 926 CACGTG CAC^GTG BbsI 220 GAAGAC GAAGAC(2/6) BciVI 573 GTATCC GTATCC(6/5) BpmI 29 CTGGAG CTGGAG(16/14) BpuAI 220 GAAGAC GAAGAC(2/6) BsaAI 926 CACGTG [CT]AC^GT[GA] BsaI 904 GGTCTC GGTCTC(1/5) BsmAI 905 GTCTC GTCTC(1/5) BsmFI 764 GGGAC GGGAC(10/14) Bsp1407I 62 TGTACA T^GTACA BsrGI 62 TGTACA T^GTACA BstXI 251 CCATTCTTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG Cac8I 650 GCTGGC GC[ACGT]^[ACGT]GC CfoI 692 GCGC GCG^C DsaI 803 CCATGG C^C[GA][CT]GG EaeI 248 TGGCCA [CT]^GGCC[GA] EcoRV 124 GATATC GAT^ATC HaeII 691 GGCGCT [GA]GCGC^[CT] HaeIII 249 GGCC GG^CC HhaI 692 GCGC GCG^C HinP1I 692 GCGC G^CGC HincII 583 GTCAAC GT[CT]^[GA]AC HindII 583 GTCAAC GT[CT]^[GA]AC MluNI 248 TGGCCA TGG^CCA MscI 248 TGGCCA TGG^CCA NcoI 803 CCATGG C^CATGG PmaCI 926 CACGTG CAC^GTG PmlI 926 CACGTG CAC^GTG PshBI 913 ATTAAT AT^TAAT RsaI 63 GTAC GT^AC SmlI 655 CTCAAG C^T[CT][GA]AG SspBI 62 TGTACA T^GTACA ################################### # TWO CUT ENZYMES ( 22 enzymes) # ################################### AcsI 664 GAATTT [GA]^AATT[CT] 985 GAATTT ApoI 664 GAATTT [GA]^AATT[CT] 985 GAATTT Asp700I 318 GAAAATCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 554 GAAACCATTC AspI 151 GACGGTGTC GAC[ACGT]^[ACGT][ACGT]GTC 754 GACCACGTC BmyI 179 GTGCCC G[AGT]GC[ACT]^C 261 GGGCTC BsgI 338 GTGCAG GTGCAG(16/14) 679 GTGCAG Bsp1286I 179 GTGCCC G[AGT]GC[ACT]^C 261 GGGCTC BsrI 28 ACTGG ACTGG(1/-1) 722 CCAGT BstYI 79 GGATCT [GA]^GATC[CT] 939 GGATCT DdeI 345 CTTAG C^T[ACGT]AG 569 CTCAG HphI 597 GGTGA GGTGA(8/7) 128 TCACC MflI 79 GGATCT [GA]^GATC[CT] 939 GGATCT MspA1I 35 CAGCTG C[AC]G^C[GT]G 648 CAGCTG NlaIV 262 GGCTCC GG[ACGT]^[ACGT]CC 778 GGAACC PflFI 151 GACGGTGTC GAC[ACGT]^[ACGT][ACGT]GTC 754 GACCACGTC PvuII 35 CAGCTG CAG^CTG 648 CAGCTG TaqI 565 TCGA T^CGA 711 TCGA TspRI 56 CACTG CA[GC]TG[ACGT][ACGT]^ 723 CAGTG Tth111I 151 GACGGTGTC GAC[ACGT]^[ACGT][ACGT]GTC 754 GACCACGTC TthHB8I 565 TCGA T^CGA 711 TCGA XhoII 79 GGATCT [GA]^GATC[CT] 939 GGATCT XmnI 318 GAAAATCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 554 GAAACCATTC ######################################### # 3 OR MORE CUT ENZYMES ( 35 enzymes) # ######################################### AluI AlwI BbvI BsaJI BsiYI BslI BssKI BstF5I BstNI DpnII EcoRII EcoT14I Fnu4HI FokI HinfI ItaI MaeII MaeIII MboI MboII MnlI MseI MslI MvaI MwoI NdeII NlaIII PleI Sau3AI ScrFI StyI Tru9I TseI Tsp45I Tsp509I