(Circular) (Six-base) MAPSORT of: pFN21AE5460.seq Check: 7845 from: 1 to: 5707 pFN21AE5460 5707 bp With 182 enzymes: SgfI * January 30, 2014 17:20 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3517 278 Size: 53 83 186 936 1981 2468 Fragments arranged by size: 2468 1981 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3050 1937 Size: 1113 4594 Acc65I G'GTAC_C Cuts at: 3028 3028 Size: 5707 AclI AA'CG_TT Cuts at: 1563 3834 4207 1563 Size: 2271 373 3063 Fragments arranged by size: 3063 2271 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2443 3770 5154 5422 1632 Size: 811 1327 1384 268 1917 Fragments arranged by size: 1917 1384 1327 811 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5510 AflIII A'CryG_T Cuts at: 1203 4606 5583 1203 Size: 3403 977 1327 Fragments arranged by size: 3403 1327 977 AgeI A'CCGG_T Cuts at: 4538 4538 Size: 5707 AhdI GACnn_n'nnGTC Cuts at: 4436 4436 Size: 5707 AleI CACnn'nnGTG Cuts at: 1166 2681 1166 Size: 1515 4192 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2199 1184 Size: 1015 4692 AlwNI CAG_nnn'CTG Cuts at: 3450 5022 3450 Size: 1572 4135 ApaLI G'TGCA_C Cuts at: 3764 4920 3764 Size: 1156 4551 ApoI r'AATT_y Cuts at: 1493 2669 2990 3016 3242 3394 5503 1493 Size: 1176 321 26 226 152 2109 1697 Fragments arranged by size: 2109 1697 1176 321 226 152 26 AseI AT'TA_AT Cuts at: 160 1032 2919 4261 160 Size: 872 1887 1342 1606 Fragments arranged by size: 1887 1606 1342 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5707 AvaI C'yCGr_G Cuts at: 1870 1943 3032 1870 Size: 73 1089 4545 Fragments arranged by size: 4545 1089 73 BamHI G'GATC_C Cuts at: 3037 3404 3037 Size: 367 5340 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3028 4483 618 Size: 325 217 627 1241 1455 1842 Fragments arranged by size: 1842 1455 1241 627 325 217 BanII G_rGCy'C Cuts at: 1391 2270 3026 1391 Size: 879 756 4072 Fragments arranged by size: 4072 879 756 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2232 928 Size: 971 333 4403 Fragments arranged by size: 4403 971 333 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3898 3932 1903 Size: 34 1961 34 3678 Fragments arranged by size: 3678 1961 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2589 3599 4809 2589 Size: 1010 1210 3487 Fragments arranged by size: 3487 1210 1010 BclI T'GATC_A Cuts at: 1548 1548 Size: 5707 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4318 136 Size: 107 122 71 631 605 2646 1525 Fragments arranged by size: 2646 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5702 5702 Size: 5707 BlpI GC'TnA_GC Cuts at: 3124 3124 Size: 5707 Bme1580I G_kGCm'C Cuts at: 1790 2188 3768 4924 1790 Size: 398 1580 1156 2573 Fragments arranged by size: 2573 1580 1156 398 BmgBI CAC'GTC Cuts at: 1318 2764 1318 Size: 1446 4261 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3438 4396 442 Size: 416 53 83 399 24 2021 958 1753 Fragments arranged by size: 2021 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5707 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2055 4367 1239 Size: 816 2312 2579 Fragments arranged by size: 2579 2312 816 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2645 3832 4697 4995 2645 Size: 1187 865 298 3357 Fragments arranged by size: 3357 1187 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2915 4370 882 Size: 617 1416 1455 2219 Fragments arranged by size: 2219 1455 1416 617 BsaAI yAC'GTr Cuts at: 493 1204 2933 3498 493 Size: 711 1729 565 2702 Fragments arranged by size: 2702 1729 711 565 BsaBI GATnn'nnATC Cuts at: 5701 5701 Size: 5707 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3514 3896 4531 275 Size: 53 83 186 936 1981 382 635 1451 Fragments arranged by size: 1981 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4140 4538 4812 4959 5370 4140 Size: 398 274 147 411 4477 Fragments arranged by size: 4477 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2658 2688 2787 2817 1178 Size: 30 165 30 1255 30 99 30 4068 Fragments arranged by size: 4068 1255 165 99 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5707 BseYI C'CCAG_C Cuts at: 4910 5406 4910 Size: 496 5211 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2364 2705 1163 Size: 573 628 341 4165 Fragments arranged by size: 4165 628 573 341 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3157 3918 4946 1540 Size: 275 186 1156 761 1028 2301 Fragments arranged by size: 2301 1156 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3768 3853 4924 3768 Size: 85 1071 4551 Fragments arranged by size: 4551 1071 85 BsmI GAATG_Cn' Cuts at: 3218 3311 3218 Size: 93 5614 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2188 2270 3026 3768 3853 4924 1391 Size: 399 398 82 756 742 85 1071 2174 Fragments arranged by size: 2174 1071 756 742 399 398 85 82 BspEI T'CCGG_A Cuts at: 5370 5370 Size: 5707 BspHI T'CATG_A Cuts at: 3596 5326 3596 Size: 1730 3977 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3062 844 Size: 2218 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3594 1394 Size: 429 1771 3507 Fragments arranged by size: 3507 1771 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4202 4376 65 Size: 1183 2954 174 1396 Fragments arranged by size: 2954 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4351 4538 1700 Size: 110 2541 187 2869 Fragments arranged by size: 2869 2541 187 110 BsrGI T'GTAC_A Cuts at: 96 2067 96 Size: 1971 3736 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5707 BssSI C'ACGA_G Cuts at: 3767 4779 3767 Size: 1012 4695 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5707 BstBI TT'CG_AA Cuts at: 4511 4511 Size: 5707 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5707 BstXI CCAn_nnnn'nTGG Cuts at: 2263 2263 Size: 5707 BstYI r'GATC_y Cuts at: 1972 2084 2944 3037 3404 3789 3806 5258 5344 Size: 112 860 93 367 385 17 1452 86 Cuts at: 5344 5356 5702 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 860 385 367 346 112 93 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2808 513 Size: 552 1743 3412 Fragments arranged by size: 3412 1743 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2194 487 Size: 683 56 968 4000 Fragments arranged by size: 4000 968 683 56 BtsI GCAGTG_nn' Cuts at: 2060 3223 4017 4037 2060 Size: 1163 794 20 3730 Fragments arranged by size: 3730 1163 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5707 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5672 DraI TTT'AAA Cuts at: 3011 3011 Size: 5707 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4714 809 Size: 507 3398 1802 Fragments arranged by size: 3398 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2253 3154 4043 Size: 54 1425 50 275 18 423 901 889 Cuts at: 4043 5672 8 Size: 1629 43 Fragments arranged by size: 1629 1425 901 889 423 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3154 1537 Size: 275 1342 4090 Fragments arranged by size: 4090 1342 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3637 1353 Size: 2284 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4294 4668 4814 387 Size: 1064 138 2705 374 146 1280 Fragments arranged by size: 2705 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2055 2443 3770 4367 5154 5422 1239 Size: 393 423 388 1327 597 787 268 1524 Fragments arranged by size: 1524 1327 787 597 423 393 388 268 EcoRI G'AATT_C Cuts at: 3016 3394 3016 Size: 378 5329 EcoRV GAT'ATC Cuts at: 2131 2131 Size: 5707 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2473 2473 Size: 5707 FspI TGC'GCA Cuts at: 4213 4213 Size: 5707 HaeII r_GCGC'y Cuts at: 1678 2700 4854 1678 Size: 1022 2154 2531 Fragments arranged by size: 2531 2154 1022 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2563 2595 2658 2690 2789 Size: 32 135 32 991 32 63 32 99 Cuts at: 2789 2816 2821 2848 4348 4380 4422 4454 1373 Size: 27 5 27 1500 32 42 32 2626 Fragments arranged by size: 2626 1500 991 135 99 63 42 32 32 32 32 32 32 27 27 5 HincII GTy'rAC Cuts at: 669 1657 1938 2590 3051 3297 669 Size: 988 281 652 461 246 3079 Fragments arranged by size: 3079 988 652 461 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5707 HpaI GTT'AAC Cuts at: 3297 3297 Size: 5707 KpnI G_GTAC'C Cuts at: 3032 3032 Size: 5707 MfeI C'AATT_G Cuts at: 3306 3306 Size: 5707 MluI A'CGCG_T Cuts at: 5583 5583 Size: 5707 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2106 4821 5005 1305 Size: 801 2715 184 2007 Fragments arranged by size: 2715 2007 801 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2255 10 Size: 54 1425 343 423 3462 Fragments arranged by size: 3462 1425 423 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2138 2261 2681 3665 4024 4183 518 Size: 648 972 123 420 984 359 159 2042 Fragments arranged by size: 2042 984 972 648 420 359 159 123 MspA1I CmG'CkG Cuts at: 1606 2042 2655 3123 3800 4948 5193 1606 Size: 436 613 468 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 613 468 436 245 NcoI C'CATG_G Cuts at: 513 1065 2808 513 Size: 552 1743 3412 Fragments arranged by size: 3412 1743 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5707 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5707 NotI GC'GGCC_GC Cuts at: 3154 3154 Size: 5707 NspI r_CATG'y Cuts at: 1132 1229 3065 4610 1132 Size: 97 1836 1545 2229 Fragments arranged by size: 2229 1836 1545 97 PasI CC'CwG_GG Cuts at: 1348 2338 1348 Size: 990 4717 PciI A'CATG_T Cuts at: 4606 4606 Size: 5707 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5707 PfoI T'CCnGG_A Cuts at: 2151 2151 Size: 5707 PmeI GTTT'AAAC Cuts at: 3011 3011 Size: 5707 PmlI CAC'GTG Cuts at: 2933 2933 Size: 5707 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3776 3808 5289 5321 3776 Size: 32 1481 32 4162 Fragments arranged by size: 4162 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5707 PsiI TTA'TAA Cuts at: 3277 3277 Size: 5707 PstI C_TGCA'G Cuts at: 830 3059 830 Size: 2229 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5707 PvuII CAG'CTG Cuts at: 2042 2655 2042 Size: 613 5094 SalI G'TCGA_C Cuts at: 1936 3049 1936 Size: 1113 4594 SbfI CC_TGCA'GG Cuts at: 3059 3059 Size: 5707 ScaI AGT'ACT Cuts at: 3955 5667 3955 Size: 1712 3995 SfcI C'TryA_G Cuts at: 826 1045 3055 4190 4871 5062 826 Size: 219 2010 1135 681 191 1471 Fragments arranged by size: 2010 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5707 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5707 SmaI CCC'GGG Cuts at: 1872 3034 1872 Size: 1162 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2660 3811 4712 4974 820 Size: 197 926 717 1151 901 262 1553 Fragments arranged by size: 1553 1151 926 901 717 262 197 SnaBI TAC'GTA Cuts at: 493 3498 493 Size: 3005 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5707 SphI G_CATG'C Cuts at: 1132 3065 1132 Size: 1933 3774 SspI AAT'ATT Cuts at: 5 52 3631 5 Size: 47 3579 2081 Fragments arranged by size: 3579 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5707 StyI C'CwwG_G Cuts at: 513 1065 2448 2598 2808 3146 513 Size: 552 1383 150 210 338 3074 Fragments arranged by size: 3074 1383 552 338 210 150 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2135 2801 3734 3751 3904 4089 4534 Size: 1255 555 666 933 17 153 185 445 Cuts at: 4534 325 Size: 1498 Fragments arranged by size: 1498 1255 933 666 555 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2067 3953 5665 Size: 274 80 33 51 1443 90 1886 1712 Cuts at: 5665 96 Size: 138 Fragments arranged by size: 1886 1712 1443 274 138 90 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2787 2819 5667 5699 2787 Size: 32 2848 32 2795 Fragments arranged by size: 2848 2795 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2159 2762 1316 Size: 843 603 4261 Fragments arranged by size: 4261 843 603 XbaI T'CTAG_A Cuts at: 3043 3043 Size: 5707 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5707 XmaI C'CCGG_G Cuts at: 1870 3032 1870 Size: 1162 4545 XmnI GAAnn'nnTTC Cuts at: 2327 2563 3836 2327 Size: 236 1273 4198 Fragments arranged by size: 4198 1273 236 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3515 276 Size: 53 83 186 936 1981 2468 Fragments arranged by size: 2468 1981 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoO109I FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PpuMI PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI