(Linear) (Six-base) MAPSORT of: KIEE5460.seq Check: 2147 from: 1 to: 1002 KIEE5460.orf With 182 enzymes: SgfI * January 29, 2014 12:01 .. AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 438 1002 Size: 438 564 AleI CACnn'nnGTG Cuts at: 0 676 1002 Size: 676 326 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 194 1002 Size: 194 808 ApoI r'AATT_y Cuts at: 0 664 985 1002 Size: 664 321 17 Fragments arranged by size: 664 321 17 AseI AT'TA_AT Cuts at: 0 914 1002 Size: 914 88 BanII G_rGCy'C Cuts at: 0 265 1002 Size: 265 737 BbsI GAAGACnn'nnnn_ Cuts at: 0 227 1002 Size: 227 775 BciVI GTATCCnnnnn_n' Cuts at: 0 584 1002 Size: 584 418 Bme1580I G_kGCm'C Cuts at: 0 183 1002 Size: 183 819 BmgBI CAC'GTC Cuts at: 0 759 1002 Size: 759 243 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 50 1002 Size: 50 952 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 640 1002 Size: 640 362 BsaI GGTCTCn'nnnn_ Cuts at: 0 910 1002 Size: 910 92 BsaAI yAC'GTr Cuts at: 0 928 1002 Size: 928 74 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 653 683 782 812 1002 Size: 653 30 99 30 190 Fragments arranged by size: 653 190 99 30 30 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 359 700 1002 Size: 359 341 302 Fragments arranged by size: 359 341 302 Bsp1286I G_dGCh'C Cuts at: 0 183 265 1002 Size: 183 82 737 Fragments arranged by size: 737 183 82 BsrGI T'GTAC_A Cuts at: 0 62 1002 Size: 62 940 BstXI CCAn_nnnn'nTGG Cuts at: 0 258 1002 Size: 258 744 BstYI r'GATC_y Cuts at: 0 79 939 1002 Size: 79 860 63 Fragments arranged by size: 860 79 63 BtgI C'CryG_G Cuts at: 0 803 1002 Size: 803 199 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 189 1002 Size: 189 813 BtsI GCAGTG_nn' Cuts at: 0 55 1002 Size: 55 947 EaeI y'GGCC_r Cuts at: 0 248 1002 Size: 248 754 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 50 438 1002 Size: 50 388 564 Fragments arranged by size: 564 388 50 EcoRV GAT'ATC Cuts at: 0 126 1002 Size: 126 876 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 468 1002 Size: 468 534 HaeII r_GCGC'y Cuts at: 0 695 1002 Size: 695 307 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 558 590 653 685 784 811 816 843 Size: 558 32 63 32 99 27 5 27 Cuts at: 843 1002 Size: 159 Fragments arranged by size: 558 159 99 63 32 32 27 27 5 HincII GTy'rAC Cuts at: 0 585 1002 Size: 585 417 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 101 1002 Size: 101 901 MscI TGG'CCA Cuts at: 0 250 1002 Size: 250 752 MslI CAynn'nnrTG Cuts at: 0 133 256 676 1002 Size: 133 123 420 326 Fragments arranged by size: 420 326 133 123 MspA1I CmG'CkG Cuts at: 0 37 650 1002 Size: 37 613 352 Fragments arranged by size: 613 352 37 NcoI C'CATG_G Cuts at: 0 803 1002 Size: 803 199 PasI CC'CwG_GG Cuts at: 0 333 1002 Size: 333 669 PfoI T'CCnGG_A Cuts at: 0 146 1002 Size: 146 856 PmlI CAC'GTG Cuts at: 0 928 1002 Size: 928 74 PvuII CAG'CTG Cuts at: 0 37 650 1002 Size: 37 613 352 Fragments arranged by size: 613 352 37 SmlI C'TyrA_G Cuts at: 0 655 1002 Size: 655 347 StyI C'CwwG_G Cuts at: 0 443 593 803 1002 Size: 443 150 210 199 Fragments arranged by size: 443 210 199 150 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 130 796 1002 Size: 130 666 206 Fragments arranged by size: 666 206 130 TatI w'GTAC_w Cuts at: 0 62 1002 Size: 62 940 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 782 814 1002 Size: 782 32 188 Fragments arranged by size: 782 188 32 Tth111I GACn'n_nGTC Cuts at: 0 154 757 1002 Size: 154 603 245 Fragments arranged by size: 603 245 154 XmnI GAAnn'nnTTC Cuts at: 0 322 558 1002 Size: 322 236 444 Fragments arranged by size: 444 322 236 Enzymes that do cut: AcuI AleI AlfI ApoI AseI BanII BbsI BciVI Bme1580I BmgBI BpmI BpuEI BsaI BsaAI BsaXI BsgI Bsp1286I BsrGI BstXI BstYI BtgI BtgZI BtsI EaeI Eco57MI EcoRV FalI HaeII Hin4I HincII MmeI MscI MslI MspA1I NcoI PasI PfoI PmlI PvuII SmlI StyI TaqII TatI TstI Tth111I XmnI Enzymes that do not cut: AarI AatII AccI Acc65I AclI AfeI AflII AflIII AgeI AhdI AloI AlwNI ApaI ApaLI AscI AsiSI AvaI AvrII BaeI BamHI BanI BbeI BbvCI BcgI BclI BfrBI BglI BglII BlpI BmrI BmtI BplI Bpu10I BsaBI BsaHI BsaWI BseRI BseYI BsiEI BsiHKAI BsiWI BsmI BsmBI BspEI BspHI BspMI BsrBI BsrDI BsrFI BssHII BssSI BstAPI BstBI BstEII BstZ17I Bsu36I ClaI CspCI DraI DraIII DrdI EagI EarI EciI EcoICRI EcoNI EcoO109I EcoRI FseI FspI FspAI HindIII HpaI KasI KpnI MfeI MluI NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI NspI PacI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PspXI PsrI PstI PvuI RsrII SacI SacII SalI SanDI SapI SbfI ScaI SexAI SfcI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SspI StuI SwaI XbaI XcmI XhoI XmaI ZraI